##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139395_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5831459
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.46406088081902	25.0	18.0	32.0	18.0	33.0
2	27.691865620593404	30.0	25.0	32.0	18.0	33.0
3	29.93402165735882	31.0	29.0	33.0	25.0	33.0
4	30.348883529833614	31.0	29.0	33.0	28.0	33.0
5	31.911361290544956	33.0	32.0	33.0	31.0	33.0
6	36.581049956794686	38.0	37.0	38.0	34.0	38.0
7	36.67251437419006	38.0	37.0	38.0	34.0	38.0
8	37.17721705665769	38.0	38.0	38.0	36.0	38.0
9	37.32536317240677	38.0	38.0	38.0	36.0	38.0
10-11	37.34517982549478	38.0	38.0	38.0	37.0	38.0
12-13	37.42463352653256	38.0	38.0	38.0	37.0	38.0
14-15	37.441226543820335	38.0	38.0	38.0	37.0	38.0
16-17	37.473684887435546	38.0	38.0	38.0	37.0	38.0
18-19	37.48373554542697	38.0	38.0	38.0	37.0	38.0
20-21	37.49434085841139	38.0	38.0	38.0	37.0	38.0
22-23	37.50369861902118	38.0	38.0	38.0	37.0	38.0
24-25	37.51889792449248	38.0	38.0	38.0	37.0	38.0
26-27	37.48643429531536	38.0	38.0	38.0	37.0	38.0
28-29	37.46734372610378	38.0	38.0	38.0	37.0	38.0
30-31	37.469483337990674	38.0	38.0	38.0	37.0	38.0
32-33	37.44580214723554	38.0	38.0	38.0	37.0	38.0
34-35	37.41975825775014	38.0	38.0	38.0	37.0	38.0
36-37	37.39425850900261	38.0	38.0	38.0	37.0	38.0
38-39	37.37336083841525	38.0	38.0	38.0	37.0	38.0
40-41	37.3869509689587	38.0	38.0	38.0	37.0	38.0
42-43	37.36055624149996	38.0	38.0	38.0	37.0	38.0
44-45	37.32794682582529	38.0	38.0	38.0	37.0	38.0
46-47	37.297878988788455	38.0	38.0	38.0	37.0	38.0
48-49	37.24769740592181	38.0	38.0	38.0	37.0	38.0
50-51	37.1789000182128	38.0	38.0	38.0	37.0	38.0
52-53	37.170580061498875	38.0	38.0	38.0	36.5	38.0
54-55	37.11130821354226	38.0	38.0	38.0	36.5	38.0
56-57	37.08645639540041	38.0	38.0	38.0	36.0	38.0
58-59	37.08843028772346	38.0	38.0	38.0	36.0	38.0
60-61	37.09483207330261	38.0	38.0	38.0	36.0	38.0
62-63	37.102882640680676	38.0	38.0	38.0	36.0	38.0
64-65	37.106958312275694	38.0	38.0	38.0	36.0	38.0
66-67	37.10088666696335	38.0	38.0	38.0	36.0	38.0
68-69	37.08996260809923	38.0	38.0	38.0	36.0	38.0
70-71	37.08494909282291	38.0	38.0	38.0	36.0	38.0
72-73	37.08386109147149	38.0	38.0	38.0	36.0	38.0
74-75	37.07645803375486	38.0	38.0	38.0	36.0	38.0
76-77	37.07103449876344	38.0	38.0	38.0	36.0	38.0
78-79	37.04989205399124	38.0	38.0	38.0	36.0	38.0
80-81	37.04772130437725	38.0	38.0	38.0	36.0	38.0
82-83	36.99586793038602	38.0	38.0	38.0	36.0	38.0
84-85	36.97644348925079	38.0	38.0	38.0	36.0	38.0
86-87	36.971795850458776	38.0	38.0	38.0	36.0	38.0
88-89	36.94769950257846	38.0	38.0	38.0	35.5	38.0
90-91	36.94590040315974	38.0	38.0	38.0	35.0	38.0
92-93	36.94729471846982	38.0	38.0	38.0	35.0	38.0
94-95	36.95037696776521	38.0	38.0	38.0	35.0	38.0
96-97	36.958570265884646	38.0	38.0	38.0	35.0	38.0
98-99	36.94959343658202	38.0	38.0	38.0	35.0	38.0
100	35.33753378884239	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	5.0
20	14.0
21	70.0
22	252.0
23	837.0
24	1915.0
25	4150.0
26	7596.0
27	13286.0
28	22361.0
29	33067.0
30	43777.0
31	55763.0
32	72063.0
33	101116.0
34	160028.0
35	326125.0
36	1165970.0
37	3823061.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.11151566014611	22.634009773540377	14.311272702080217	23.94320186423329
2	14.186416589678084	20.514183706911616	41.8076742541654	23.4917254492449
3	17.713114333822805	26.073903632007017	31.617319096301628	24.59566293786855
4	12.483496840156127	18.508335564050093	39.29670087708754	29.711466718706248
5	13.162109173707643	37.55134006772576	35.84602069567839	13.440530062888206
6	29.22095482451304	38.7527032257279	18.725296705335662	13.301045244423392
7	25.833432079347553	33.0678651774796	23.193526697178186	17.905176045994665
8	23.95186178964818	37.11630314128934	21.279700328854236	17.65213474020824
9	25.186407038101443	17.107656934568176	21.335998418234613	36.36993760909577
10-11	24.008391381985195	27.056299289766077	29.03624118766847	19.899068140580255
12-13	24.98407345400182	24.257059854146277	29.039868067322434	21.71899862452947
14-15	22.140171096118486	25.740873767611163	26.62114232475955	25.497812811510805
16-17	21.71709515577491	28.924168034106046	27.29964319392454	22.059093616194506
18-19	21.603778745593512	27.912457242690035	29.149403262545448	21.334360749171005
20-21	22.665378007241163	27.12359143619961	28.84386945601069	21.367161100548532
22-23	22.1825995997856	27.2926259763447	28.71788280287962	21.806891620990086
24-25	22.028913672744764	27.40815854883668	29.01235843624169	21.550569342176857
26-27	22.087687671410052	27.52445400723751	28.8089385336867	21.57891978766574
28-29	21.736943001731714	27.74649453408825	28.871824764111608	21.644737700068433
30-31	22.54916037779435	27.347770760148745	28.782748323220243	21.32032053883666
32-33	21.709247829673167	27.850492200004144	28.78782182150782	21.652438148814866
34-35	21.962097740559994	27.729108358743794	28.945506973921546	21.363286926774663
36-37	21.90525837315231	27.54938919610172	28.971748875158404	21.573603555587564
38-39	21.786108534155257	27.22065090060073	29.225787092560356	21.767453472683655
40-41	22.069400848666106	27.14435969735622	29.16731963611476	21.618919817862913
42-43	22.272488074153816	27.08708369299611	29.025458526513866	21.614969706336204
44-45	21.99810241572019	27.58951810757496	28.754966368556488	21.657413108148358
46-47	22.34495763210724	27.045914872136535	28.34064119079878	22.268486304957438
48-49	22.26059636753813	27.07386683045848	28.532059247037267	22.13347755496612
50-51	21.884890304662044	27.373771068071584	28.565817157215907	22.175521470050466
52-53	22.091655105271574	27.415285128144685	28.649816311967285	21.843243454616452
54-55	22.163032973740307	27.38022685341459	28.272815969444608	22.18392420340049
56-57	22.40733032955663	27.75792651723154	27.86609531353746	21.968647839674365
58-59	21.98549114313999	28.03863446695008	28.466206694906187	21.509667695003746
60-61	22.232571992825143	28.55006037541843	27.700517056411417	21.51685057534501
62-63	22.050431349707644	28.661995034972183	27.67085508769929	21.616718527620886
64-65	21.87311698539268	28.635404352559608	27.745274809842673	21.74620385220504
66-67	22.095824138259697	28.665177244403615	27.53035532779013	21.708643289546554
68-69	22.130828589755055	28.194800710485463	27.887038180992956	21.787332518766526
70-71	22.152044690861057	27.64475823521679	28.0633776946541	22.13981937926805
72-73	22.3590828193929	27.53832828204462	28.1986791160238	21.903909782538676
74-75	22.10082756168581	27.734873570684705	28.060302177426422	22.10399669020307
76-77	22.16812022004562	27.696322595960325	27.920135412688747	22.21542177130531
78-79	22.25192593140066	27.704859775848593	27.88025007268403	22.162964220066712
80-81	22.16805142928526	27.610209598731668	27.973658438141126	22.248080533841947
82-83	22.35803992415879	27.483091689723853	28.122365811412593	22.036502574704762
84-85	22.65787509469754	27.48043060830862	27.84601040662543	22.01568389036841
86-87	22.103824371431593	27.65533829141068	27.96897934337063	22.271857993787098
88-89	22.403470031614937	27.597275758891193	27.927123545242395	22.072130664251475
90-91	22.40126440812866	27.59610214748242	27.978079098024782	22.024554346364134
92-93	22.489138805455266	27.63970667291209	27.810319724014498	22.060834797618146
94-95	22.2775141057421	27.732520524503883	27.822458024064517	22.167507345689497
96-97	22.304385470071004	27.8277201817479	27.956469215784697	21.911425132396406
98-99	23.33788218939447	29.7908251542	28.112452695523615	18.75883996088191
100	22.52697196806708	30.66692410546585	21.865564408297935	24.94053951816914
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1107.0
1	1150.0
2	1480.5
3	1884.5
4	2460.5
5	3436.0
6	4122.5
7	4878.0
8	5826.0
9	6415.0
10	6570.0
11	6794.5
12	7847.5
13	10186.5
14	14639.5
15	19617.0
16	23283.0
17	25388.0
18	25260.5
19	23414.0
20	21128.0
21	19291.0
22	19505.5
23	22609.5
24	28167.0
25	36795.5
26	49986.0
27	64700.0
28	74695.5
29	85497.5
30	96431.5
31	105533.0
32	119048.5
33	134374.0
34	150206.5
35	164670.0
36	181036.5
37	197435.5
38	203988.5
39	207439.0
40	216494.0
41	223449.5
42	228053.0
43	243345.5
44	262321.5
45	269743.5
46	264483.5
47	256615.0
48	249584.5
49	241909.0
50	233276.0
51	224227.5
52	215140.0
53	203970.5
54	191291.0
55	178051.5
56	164223.0
57	149216.0
58	133129.0
59	115880.5
60	95218.0
61	72480.0
62	53998.0
63	40044.0
64	29042.0
65	20635.0
66	14598.0
67	10625.5
68	7843.0
69	5662.5
70	4167.0
71	2988.5
72	2133.5
73	1457.0
74	936.0
75	602.5
76	399.5
77	250.5
78	154.5
79	98.5
80	63.5
81	41.5
82	27.5
83	16.0
84	7.5
85	4.5
86	4.5
87	4.0
88	3.0
89	1.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.008711370516366487
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	5.1445745488293865E-5
22-23	8.574674698278634E-6
24-25	3.8589146502408393E-4
26-27	0.002693000899239313
28-29	0.0061941103790469545
30-31	0.006852467575291273
32-33	0.0024777802048934956
34-35	9.315093115999561E-4
36-37	4.328226372471926E-5
38-39	5.571242569028784E-4
40-41	1.138771580816429E-4
42-43	9.697771593146391E-5
44-45	1.9542539355787297E-4
46-47	3.5838921965227285E-5
48-49	8.224469822205941E-4
50-51	5.922473545677396E-4
52-53	0.0
54-55	0.007536551117004724
56-57	0.062114609518367066
58-59	0.07401066550751406
60-61	0.04442945974687154
62-63	0.009336778757756892
64-65	9.653381811329672E-5
66-67	3.902244483226251E-5
68-69	4.935547670085883E-4
70-71	0.0029887644447785283
72-73	0.012405549463052374
74-75	0.010620563946834622
76-77	0.016428474426272682
78-79	0.0065252399094096565
80-81	0.007614719220657185
82-83	0.04018266939346942
84-85	0.025113799253592528
86-87	0.008932772475338547
88-89	0.0021495852506866063
90-91	1.1088185671225537E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	42.0
20-21	202.0
22-23	456.0
24-25	612.0
26-27	1325.0
28-29	3596.0
30-31	10623.0
32-33	13501.0
34-35	18249.0
36-37	29876.0
38-39	36387.0
40-41	35632.0
42-43	40575.0
44-45	47440.0
46-47	48858.0
48-49	45897.0
50-51	41383.0
52-53	39434.0
54-55	42733.0
56-57	44691.0
58-59	44472.0
60-61	46778.0
62-63	47226.0
64-65	52763.0
66-67	58586.0
68-69	58567.0
70-71	72050.0
72-73	46891.0
74-75	45573.0
76-77	48064.0
78-79	49088.0
80-81	49618.0
82-83	46172.0
84-85	45723.0
86-87	47170.0
88-89	49247.0
90-91	49941.0
92-93	49433.0
94-95	55877.0
96-97	173144.0
98-99	386834.0
100-101	3806730.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.45379188164169
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.77731383549738	74.08803472638789
2	7.607853817542411	12.698084983102751
3	2.008929182924502	5.029582738102136
4	0.7503550452733745	2.5047989514233606
5	0.3513832762122998	1.4662133401855342
6	0.16627378261901687	0.8325706590036459
7	0.10353818536492916	0.6048457921273346
8	0.06429887518927212	0.42927879586153356
9	0.04324920823934693	0.3248379381107018
>10	0.12313340724174836	1.5224324333010864
>50	0.002290841606830252	0.1334470779799329
>100	0.0011426515653982269	0.17556850489565407
>500	1.699219484371108E-4	0.10082428418744438
>1k	6.796877498404062E-5	0.08947977533104928
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	1.7148367158201746E-5	0.0
7	0.0	0.0	0.0	1.7148367158201746E-5	0.0
8	0.0	0.0	0.0	1.7148367158201746E-5	0.0
9	0.0	0.0	0.0	8.574183579100874E-5	0.0
10-11	8.574183579100873E-6	0.0	0.0	2.6579969095212705E-4	0.0
12-13	1.7148367158201746E-5	0.0	0.0	3.515415267431358E-4	0.0
14-15	1.7148367158201746E-5	0.0	0.0	4.544317296923463E-4	0.0
16-17	1.7148367158201746E-5	0.0	0.0	5.401735654833549E-4	0.0
18-19	3.429673431640349E-5	0.0	0.0	5.487477490624559E-4	0.0
20-21	3.429673431640349E-5	0.0	0.0	5.830444833788594E-4	0.0
22-23	4.287091789550437E-5	0.0	0.0	5.830444833788594E-4	0.0
24-25	1.286127536865131E-4	0.0	0.0	6.08767034116162E-4	0.0
26-27	1.8863203874021922E-4	0.0	0.0	6.859346863280699E-4	0.0
28-29	2.6579969095212705E-4	0.0	0.0	0.0010717729473876093	0.0
30-31	3.086706088476314E-4	0.0	0.0	0.002152120078354319	0.0
32-33	3.258189760058332E-4	0.0	0.0	0.004501446379027958	0.0
34-35	3.258189760058332E-4	0.0	0.0	0.007571004100346071	0.0
36-37	3.258189760058332E-4	0.0	0.0	0.012166766498744139	0.0
38-39	3.258189760058332E-4	0.0	0.0	0.01907755846349944	0.0
40-41	3.258189760058332E-4	0.0	0.0	0.02609124063120396	0.0
42-43	3.258189760058332E-4	0.0	0.0	0.03355078034502172	0.0
44-45	3.4296734316403496E-4	0.0	0.0	0.04159336454221833	0.0
46-47	3.4296734316403496E-4	0.0	0.0	0.051247895252285916	0.0
48-49	3.6868989390133756E-4	0.0	0.0	0.061039612899619115	0.0
50-51	4.0298662821774105E-4	0.0	0.0	0.06973383504882741	0.0
52-53	4.1156081179684193E-4	0.0	0.0	0.07798219965192245	0.0
54-55	4.287091789550437E-4	0.0	0.0	0.08606765476701457	0.0
56-57	4.458575461132454E-4	0.0	0.0	0.09194954470227776	0.0
58-59	4.458575461132454E-4	0.0	0.0	0.09798576994196478	0.0
60-61	4.458575461132454E-4	0.0	0.0	0.10347324743258934	0.0
62-63	4.544317296923463E-4	0.0	0.0	0.10926082134848243	0.0
64-65	4.630059132714472E-4	0.0	0.0	0.11514271128374563	0.0
66-67	4.7158009685054807E-4	0.0	0.0	0.12063018877437018	0.0
68-69	4.801542804296489E-4	0.0	0.0	0.1256718087188815	0.0
70-71	4.801542804296489E-4	0.0	0.0	0.1305848159097063	0.0
72-73	4.801542804296489E-4	0.0	0.0	0.13470042402767474	0.0
74-75	4.801542804296489E-4	0.0	0.0	0.13794146542057484	0.0
76-77	4.801542804296489E-4	0.0	0.0	0.1413196937507406	0.0
78-79	4.801542804296489E-4	0.0	0.0	0.14374618770362613	0.0
80-81	4.801542804296489E-4	0.0	0.0	0.14448356749142882	0.0
82-83	5.315993819042541E-4	0.0	0.0	0.14498944432259575	0.0
84-85	5.315993819042541E-4	0.0	0.0	0.14521237309565238	0.0
86-87	5.315993819042541E-4	0.0	0.0	0.1452981149314434	0.0
88	5.315993819042541E-4	0.0	0.0	0.1452981149314434	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7615	0.0	50.52685	1
CTTATAC	6405	0.0	43.573914	1
GTATCAA	13870	0.0	36.987453	1
TATACAC	9140	0.0	34.02858	3
ATCAACG	16980	0.0	30.084908	3
TCAACGC	17145	0.0	29.795378	4
CAACGCA	17500	0.0	29.28834	5
TATCAAC	17825	0.0	29.019741	2
TTATACA	10295	0.0	28.764898	2
AACGCAG	18260	0.0	28.139328	6
ACACATC	11260	0.0	25.010954	6
ACATCTC	11345	0.0	24.786009	8
ACGCAGA	20700	0.0	24.760674	7
CGCAGAG	20940	0.0	24.517576	8
TACACAT	12440	0.0	24.145483	5
GTGGTAT	3625	0.0	21.627893	1
CACATCT	12890	0.0	21.583775	7
ATACACA	15135	0.0	21.506914	4
CATCTCC	13070	0.0	21.449514	9
GCAGAGT	24360	0.0	21.057962	9
>>END_MODULE