FastQCFastQC Report
Fri 27 May 2016
SRR2139394_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139394_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6022640
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74900.050.74861
GTATCAA135450.037.1220931
ATCAACG160150.030.9395313
CAACGCA162150.030.5842825
TCAACGC162500.030.465794
AACGCAG169000.029.3952186
TATCAAC171750.029.0521472
ACGCAGA189300.026.1752037
CGCAGAG191850.025.8272918
GCAGAGT231350.021.4545769
GAGTACT127400.020.78878612-13
CAGAGTA229050.019.14091110-11
TGGTATC35350.019.1104872
GTGGTAT38400.019.040091
AGAGTAC212350.018.10948610-11
GTACTTT147800.017.94842714-15
TCGACGG3205.3217198E-516.10449694
TACATGG175550.016.0992162
GTACATG179050.016.0233141
ACATGGG174200.015.8295593
Produced by FastQC (version 0.11.5)