FastQCFastQC Report
Fri 27 May 2016
SRR2139393_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139393_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5303693
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63250.047.86481
GTATCAA113050.037.15831
ATCAACG132900.031.4108473
TCAACGC132600.031.3850444
CAACGCA136250.030.6385445
TATCAAC141300.029.7595712
AACGCAG141800.029.4997486
ACGCAGA163200.025.6052887
CGCAGAG165300.025.2540958
GCAGAGT198150.021.1105989
GTGGTAT32350.019.8552021
GAGTACT108150.019.61630212-13
TGGTATC30450.019.4065862
CAGAGTA193350.018.56773410-11
AGAGTAC179750.017.93602210-11
TATACCG3452.3101165E-1017.3743655
GTACTTT122100.017.34014314-15
GTACATG148850.016.7141651
TACATGG147900.016.474092
AGTACTT121800.016.1172912-13
Produced by FastQC (version 0.11.5)