FastQCFastQC Report
Fri 27 May 2016
SRR2139392_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139392_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5446407
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79900.051.0600931
GTATCAA143950.037.420971
ATCAACG167800.031.4103453
TCAACGC167900.031.3916364
CAACGCA171350.030.8846285
TATCAAC173900.030.5325572
AACGCAG178100.029.83446
ACGCAGA199500.026.6555987
CGCAGAG202150.026.3061668
GTGGTAT37750.022.3635481
GCAGAGT239800.022.2116839
GTACATG175950.021.3113121
TACATGG173500.020.970062
GAGTACT138250.020.95275112-13
TGGTATC38250.020.2786272
ACATGGG175450.020.173733
CAGAGTA231200.020.08170710-11
ACCGAAC7050.019.4500358
AGAGTAC216250.018.52742410-11
GTACTTT155250.018.52065814-15
Produced by FastQC (version 0.11.5)