##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139392_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5446407
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.68365878642562	30.0	18.0	33.0	18.0	34.0
2	31.576603437826076	33.0	32.0	33.0	27.0	34.0
3	32.35596182951439	33.0	33.0	33.0	30.0	34.0
4	32.774713861817524	33.0	33.0	34.0	31.0	34.0
5	33.07275824226871	33.0	33.0	34.0	32.0	34.0
6	36.25830405256163	38.0	36.0	38.0	33.0	38.0
7	36.97167343534921	38.0	38.0	38.0	35.0	38.0
8	36.48139608369334	38.0	37.0	38.0	34.0	38.0
9	37.07164760180427	38.0	38.0	38.0	36.0	38.0
10-11	37.25397284852197	38.0	38.0	38.0	36.5	38.0
12-13	37.32961023662021	38.0	38.0	38.0	36.0	38.0
14-15	37.30542273098577	38.0	38.0	38.0	36.5	38.0
16-17	37.38111253896376	38.0	38.0	38.0	37.0	38.0
18-19	37.402151087863984	38.0	38.0	38.0	37.0	38.0
20-21	37.43015172661114	38.0	38.0	38.0	37.0	38.0
22-23	37.44480412450063	38.0	38.0	38.0	37.0	38.0
24-25	37.46818318465597	38.0	38.0	38.0	37.0	38.0
26-27	37.43411080317475	38.0	38.0	38.0	37.0	38.0
28-29	37.41540896887406	38.0	38.0	38.0	37.0	38.0
30-31	37.42392902255508	38.0	38.0	38.0	37.0	38.0
32-33	37.40614389684209	38.0	38.0	38.0	37.0	38.0
34-35	37.38248666030336	38.0	38.0	38.0	37.0	38.0
36-37	37.353950098046695	38.0	38.0	38.0	37.0	38.0
38-39	37.35457463421916	38.0	38.0	38.0	37.0	38.0
40-41	37.34331903562479	38.0	38.0	38.0	37.0	38.0
42-43	37.306308650415446	38.0	38.0	38.0	37.0	38.0
44-45	37.27597523696164	38.0	38.0	38.0	37.0	38.0
46-47	37.24612929163302	38.0	38.0	38.0	37.0	38.0
48-49	37.19022164767997	38.0	38.0	38.0	37.0	38.0
50-51	37.11620893697056	38.0	38.0	38.0	36.5	38.0
52-53	37.10619413606274	38.0	38.0	38.0	36.0	38.0
54-55	37.047386339775244	38.0	38.0	38.0	36.0	38.0
56-57	37.017446507166284	38.0	38.0	38.0	36.0	38.0
58-59	37.02117039660662	38.0	38.0	38.0	36.0	38.0
60-61	37.0361762583713	38.0	38.0	38.0	36.0	38.0
62-63	37.044933117143145	38.0	38.0	38.0	36.0	38.0
64-65	37.05124630774607	38.0	38.0	38.0	36.0	38.0
66-67	37.052176646037985	38.0	38.0	38.0	36.0	38.0
68-69	37.04102396926844	38.0	38.0	38.0	36.0	38.0
70-71	37.033238509138556	38.0	38.0	38.0	36.0	38.0
72-73	37.036844817543844	38.0	38.0	38.0	36.0	38.0
74-75	37.02439076699318	38.0	38.0	38.0	36.0	38.0
76-77	37.013001816911284	38.0	38.0	38.0	36.0	38.0
78-79	36.986065194504974	38.0	38.0	38.0	36.0	38.0
80-81	36.97950723611541	38.0	38.0	38.0	36.0	38.0
82-83	36.92037139634006	38.0	38.0	38.0	35.5	38.0
84-85	36.90157357806458	38.0	38.0	38.0	35.0	38.0
86-87	36.89300793234219	38.0	38.0	38.0	35.0	38.0
88-89	36.869996653402225	38.0	38.0	38.0	35.0	38.0
90-91	36.86642153722749	38.0	38.0	38.0	35.0	38.0
92-93	36.864738962261676	38.0	38.0	38.0	35.0	38.0
94-95	36.86740447303573	38.0	38.0	38.0	35.0	38.0
96-97	36.87857364151364	38.0	38.0	38.0	35.0	38.0
98-99	36.86703396985374	38.0	38.0	38.0	35.0	38.0
100	35.253035307016525	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	16.0
21	77.0
22	313.0
23	850.0
24	2097.0
25	4322.0
26	8259.0
27	13465.0
28	21509.0
29	31964.0
30	43226.0
31	56076.0
32	72639.0
33	98613.0
34	148401.0
35	275763.0
36	848946.0
37	3819869.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.53168722058414	18.273386472953636	13.502644954738049	22.692281351724173
2	14.744008653590676	20.347340822168235	42.602408381981625	22.306242142259464
3	18.4038210879209	26.179883361636396	30.194401556842887	25.22189399359982
4	12.693854866153043	18.242724056428393	38.83964235504251	30.223778722376053
5	13.819808178125506	37.44110566837917	35.231557244987386	13.50752890850794
6	30.080767008414906	37.61169519648458	18.823437176105273	13.484100618995237
7	26.845221078777254	32.78256656177182	22.872087965515615	17.500124393935305
8	24.597298732907767	36.49147043179109	20.96376565320954	17.94746518209161
9	25.80567335492922	17.34213032555224	21.229702444198534	35.622493875320004
10-11	24.46661624810632	26.731779317998082	28.614883169766784	20.18672126412881
12-13	25.384028406250213	24.308319227703695	28.76438540123792	21.543266964808176
14-15	22.45183843219943	25.587244948825894	26.718522872051242	25.24239374692343
16-17	21.896545741072966	28.67248995530448	27.337701717848116	22.093262585774436
18-19	21.847283906619538	27.72941867914021	29.257150631599877	21.16614678264037
20-21	22.76775816595584	26.911444213106893	29.13829481837044	21.182502802566823
22-23	22.48169396501506	26.72078381421228	29.00889473569691	21.78862748507575
24-25	22.211887701857513	26.830001261566427	29.109690354305513	21.848420682270547
26-27	22.057048906763576	27.03938119528113	29.05005390904143	21.853515988913866
28-29	22.04008330147839	27.136056478468497	29.071109877641195	21.75275034241192
30-31	22.35322095490489	26.91769683904518	28.99440149396491	21.734680712085016
32-33	22.103762937100573	27.119071086874946	28.90247237861297	21.87469359741151
34-35	22.253798895933524	27.150158788448802	28.906261911905883	21.68978040371179
36-37	22.265875689954424	27.003868133311848	28.88331460548538	21.84694157124835
38-39	22.043180607618183	27.231985983713685	28.96920992164465	21.755623487023485
40-41	22.341875744318195	27.03933908067303	28.81385659432687	21.80492858068191
42-43	22.25580979027645	27.124062199951975	28.64455296491538	21.97557504485619
44-45	22.288903279112684	27.25615122505119	28.494253678679645	21.960691817156484
46-47	22.332330930148693	27.245141658231052	28.274557395842475	22.14797001577778
48-49	22.271965857272296	27.3589087640378	28.253392292536024	22.115733086153888
50-51	22.269964788119662	27.554728576799892	28.065505164076725	22.109801471003724
52-53	22.450465945134205	27.415873779802304	27.956028674092043	22.177631600971452
54-55	22.469773759047943	27.300132210368172	28.024205582660635	22.205888447923254
56-57	22.42594573074347	27.401503639782	27.985969306433812	22.18658132304072
58-59	22.36589642304182	27.428922261535703	28.054210368935596	22.15097094648688
60-61	22.437416311107796	27.374892241433983	28.10624499896237	22.081446448495846
62-63	22.373655508111902	27.433210744915538	28.037201565759773	22.155932181212783
64-65	22.319222828596192	27.44300504737828	28.0383418623773	22.199430261648228
66-67	22.445617248424043	27.513110950670256	27.85160515470096	22.189666646204746
68-69	22.3749244146675	27.450319048425847	27.989629760487617	22.185126776419033
70-71	22.38935708499696	27.23929410635345	28.00781962798091	22.363529180668674
72-73	22.498666052636715	27.219202613566303	27.946322576681844	22.335808757115142
74-75	22.30028204559897	27.373317413086845	27.951625421248284	22.37477512006591
76-77	22.45036565878406	27.326210245041338	27.833599300194923	22.389824795979678
78-79	22.51592420471456	27.37554681739716	27.73337013530745	22.375158842580834
80-81	22.454710503168513	27.372684430788834	27.746385888099685	22.42621917794296
82-83	22.48180341305639	27.356933755187473	27.814910381279457	22.346352450476683
84-85	22.657524949345202	27.310542949238986	27.72066454776133	22.31126755365448
86-87	22.385869283930035	27.424083108826547	27.80045546048635	22.389592146757067
88-89	22.51607858413366	27.430307995039083	27.711785615059192	22.34182780576807
90-91	22.5656340901355	27.4345278850735	27.67930713484598	22.32053088994502
92-93	22.62927327456461	27.44098978246847	27.666922961868885	22.262813981098034
94-95	22.498860981408406	27.589125917309907	27.611794128720728	22.300218972560955
96-97	22.551949231721355	27.613640667162986	27.80050306156152	22.033907039554137
98-99	23.64777373163719	29.5274973375601	27.987392196588324	18.83733673421439
100	22.595378835943773	30.40098994040697	21.886960262457976	25.11667096119128
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	914.0
1	832.5
2	1058.0
3	1516.5
4	1969.5
5	2861.5
6	3731.0
7	4791.5
8	6265.0
9	7127.0
10	7069.0
11	6908.0
12	7598.5
13	9436.5
14	12753.0
15	16648.5
16	19956.0
17	22335.0
18	22779.0
19	21373.0
20	19221.0
21	17358.0
22	17195.5
23	19516.5
24	24119.0
25	31195.0
26	41317.5
27	52344.5
28	62446.5
29	73959.0
30	85692.5
31	96595.5
32	108715.5
33	121494.0
34	134740.5
35	147486.5
36	161403.0
37	174209.0
38	181959.0
39	188860.5
40	198621.0
41	206799.0
42	211509.0
43	216643.5
44	221299.5
45	225926.5
46	228789.0
47	229381.5
48	229010.5
49	225569.5
50	220475.0
51	215129.5
52	207897.0
53	197838.0
54	187196.0
55	175135.5
56	161515.5
57	146865.0
58	131642.0
59	116122.5
60	98044.5
61	79345.0
62	62582.5
63	48118.0
64	36562.5
65	27497.5
66	20152.0
67	15302.5
68	11791.0
69	8620.0
70	6147.0
71	4434.0
72	3240.5
73	2227.0
74	1480.5
75	985.5
76	633.5
77	395.0
78	242.0
79	150.0
80	90.5
81	59.0
82	43.5
83	29.0
84	21.0
85	16.0
86	9.5
87	5.5
88	2.5
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.006316090589630926
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	8.262412760396823E-5
22-23	0.0
24-25	4.5908319983193886E-4
26-27	0.003140501347063874
28-29	0.007348833799785248
30-31	0.007705754920763599
32-33	0.0025340925290252654
34-35	8.501418859085563E-4
36-37	1.2989325279704243E-4
38-39	6.439894042276691E-4
40-41	8.45481810961916E-5
42-43	1.418525029023022E-4
44-45	2.096584501837704E-4
46-47	7.69131299846318E-5
48-49	0.0010182665379577763
50-51	5.083592840971724E-4
52-53	0.0
54-55	0.008377711242997547
56-57	0.06762741806466947
58-59	0.08089916315305269
60-61	0.04884866357420275
62-63	0.009536433263569843
64-65	4.120475280341687E-5
66-67	0.0
68-69	5.238948987201143E-4
70-71	0.0032138479634247186
72-73	0.012370078188491626
74-75	0.011365703832769098
76-77	0.015943536886023343
78-79	0.0066865095129003665
80-81	0.007942472198861829
82-83	0.044308953151365585
84-85	0.02768257479216416
86-87	0.008795624749821221
88-89	0.0022455378813647964
90-91	1.1569656447150561E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	26.0
20-21	194.0
22-23	464.0
24-25	510.0
26-27	1455.0
28-29	3678.0
30-31	10943.0
32-33	14034.0
34-35	19171.0
36-37	30090.0
38-39	34830.0
40-41	34653.0
42-43	38775.0
44-45	45324.0
46-47	45652.0
48-49	42266.0
50-51	37644.0
52-53	34441.0
54-55	36672.0
56-57	37130.0
58-59	39097.0
60-61	38318.0
62-63	37509.0
64-65	39443.0
66-67	41561.0
68-69	42339.0
70-71	41673.0
72-73	39014.0
74-75	39394.0
76-77	40658.0
78-79	42467.0
80-81	42425.0
82-83	39799.0
84-85	39105.0
86-87	41019.0
88-89	42082.0
90-91	43087.0
92-93	42818.0
94-95	51020.0
96-97	168041.0
98-99	375649.0
100-101	3651937.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.89511063824476
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.45366182616405	80.3832972448331
2	6.249956957056766	10.986813164495441
3	1.3503119326034898	3.5605745013697754
4	0.4509255791258492	1.5853661466752444
5	0.1972973900640888	0.8670737964160008
6	0.09927711398626995	0.52355837506013
7	0.0600404520866517	0.369408352524854
8	0.03667123226906273	0.25785776140240324
9	0.022712729871061293	0.17967041144221493
>10	0.07714184211159603	1.031510397353383
>50	0.0013866464225168668	0.08126027440239078
>100	4.957009050537135E-4	0.08086031067528116
>500	9.044800141024602E-5	0.056683050833113535
>1k	3.0149332059645225E-5	0.03606621251667359
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.672145691645887E-5
2	0.0	1.8360728458229434E-5	0.0	0.0	3.672145691645887E-5
3	0.0	1.8360728458229434E-5	0.0	0.0	3.672145691645887E-5
4	0.0	7.344291383291774E-5	0.0	1.8360728458229434E-5	3.672145691645887E-5
5	0.0	7.344291383291774E-5	0.0	1.8360728458229434E-5	3.672145691645887E-5
6	0.0	7.344291383291774E-5	0.0	1.8360728458229434E-5	3.672145691645887E-5
7	0.0	7.344291383291774E-5	0.0	1.8360728458229434E-5	3.672145691645887E-5
8	0.0	7.344291383291774E-5	0.0	1.8360728458229434E-5	5.50821853746883E-5
9	0.0	7.344291383291774E-5	0.0	3.672145691645887E-5	5.50821853746883E-5
10-11	9.180364229114717E-6	7.344291383291774E-5	0.0	4.590182114557358E-5	9.180364229114718E-5
12-13	1.8360728458229434E-5	7.344291383291774E-5	0.0	5.50821853746883E-5	1.2852509920760605E-4
14-15	1.8360728458229434E-5	1.5606619189495019E-4	0.0	6.426254960380302E-5	1.652465561240649E-4
16-17	1.8360728458229434E-5	1.652465561240649E-4	0.0	1.1934473497849133E-4	1.652465561240649E-4
18-19	1.8360728458229434E-5	1.652465561240649E-4	0.0	1.4688582766583547E-4	1.744269203531796E-4
20-21	1.8360728458229434E-5	1.652465561240649E-4	0.0	1.4688582766583547E-4	2.0196801304052378E-4
22-23	1.8360728458229434E-5	2.0196801304052378E-4	0.0	1.744269203531796E-4	2.478698341860974E-4
24-25	1.8360728458229434E-5	2.111483772696385E-4	0.0	2.570501984152121E-4	2.9377165533167094E-4
26-27	1.8360728458229434E-5	2.203287414987532E-4	0.0	3.488538407063592E-4	3.213127480190151E-4
28-29	3.672145691645887E-5	2.203287414987532E-4	0.0	7.344291383291773E-4	3.304931122481298E-4
30-31	5.50821853746883E-5	2.3868946995698264E-4	0.0	0.002047221223092582	3.3967347647724457E-4
32-33	5.50821853746883E-5	2.754109268734415E-4	0.0	0.004287230094996573	3.8557529762281814E-4
34-35	5.50821853746883E-5	3.1213238378990037E-4	0.0	0.008390852905410851	4.314771187683917E-4
36-37	5.50821853746883E-5	3.58034204935474E-4	0.0	0.014716123859270892	4.5901821145573584E-4
38-39	5.50821853746883E-5	3.763949333937034E-4	0.0	0.033379804337061114	4.957396683721948E-4
40-41	5.50821853746883E-5	4.7737893991396527E-4	0.0	0.05313594815811598	5.324611252886536E-4
42-43	5.50821853746883E-5	4.8655930414308E-4	0.0	0.06273860914176997	5.50821853746883E-4
44-45	5.50821853746883E-5	5.600022179759977E-4	0.0	0.07473734518922291	5.600022179759977E-4
46-47	5.50821853746883E-5	6.242647675798007E-4	0.0	0.08654329358786444	5.783629464342272E-4
48-49	5.50821853746883E-5	6.242647675798007E-4	0.0	0.09819317579461101	5.875433106633419E-4
50-51	5.50821853746883E-5	6.977076814127186E-4	0.0	0.11045814240470828	5.875433106633419E-4
52-53	5.50821853746883E-5	6.977076814127186E-4	0.0	0.14598615197138223	6.059040391215713E-4
54-55	5.50821853746883E-5	6.977076814127186E-4	0.0	0.19006108063536198	6.334451318089154E-4
56-57	5.50821853746883E-5	6.977076814127186E-4	0.0	0.25260690212832054	6.426254960380302E-4
58-59	5.50821853746883E-5	7.068880456418333E-4	0.0	0.29257820798188605	6.518058602671449E-4
60-61	5.50821853746883E-5	7.344291383291774E-4	0.0	0.30692711727199234	6.79346952954489E-4
62-63	6.426254960380302E-5	7.711505952456363E-4	0.0	0.3196143806366289	6.79346952954489E-4
64-65	7.344291383291774E-5	8.262327806203246E-4	0.0	0.3262976857954244	7.252487741000626E-4
66-67	7.344291383291774E-5	8.262327806203246E-4	0.0	0.3320170527101629	7.344291383291774E-4
68-69	7.344291383291774E-5	8.262327806203246E-4	0.0	0.3378190429029634	7.436095025582922E-4
70-71	7.344291383291774E-5	8.44593509078554E-4	0.0	0.34263873412324863	7.711505952456363E-4
72-73	7.344291383291774E-5	8.44593509078554E-4	0.0	0.34627415835797803	8.44593509078554E-4
74-75	7.344291383291774E-5	8.721346017658981E-4	0.0	0.3491935141828365	8.44593509078554E-4
76-77	7.344291383291774E-5	8.813149659950128E-4	0.0	0.35240664166302665	8.629542375367834E-4
78-79	7.344291383291774E-5	8.813149659950128E-4	0.0	0.35448140397880656	8.996756944532423E-4
80-81	9.180364229114718E-5	8.813149659950128E-4	0.0	0.35508730801792815	9.272167871405865E-4
82-83	9.180364229114718E-5	9.363971513697011E-4	0.0	0.35546370295132185	0.0010006597009735042
84-85	9.180364229114718E-5	9.363971513697011E-4	0.0	0.35560140841475857	0.0010465615221190777
86-87	9.180364229114718E-5	9.547578798279305E-4	0.0	0.35569321205704973	0.0010465615221190777
88	9.180364229114718E-5	9.914793367443895E-4	0.0	0.3557023924212788	0.0010649222505773072
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7990	0.0	51.060093	1
GTATCAA	14395	0.0	37.42097	1
ATCAACG	16780	0.0	31.410345	3
TCAACGC	16790	0.0	31.391636	4
CAACGCA	17135	0.0	30.884628	5
TATCAAC	17390	0.0	30.532557	2
AACGCAG	17810	0.0	29.8344	6
ACGCAGA	19950	0.0	26.655598	7
CGCAGAG	20215	0.0	26.306166	8
GTGGTAT	3775	0.0	22.363548	1
GCAGAGT	23980	0.0	22.211683	9
GTACATG	17595	0.0	21.311312	1
TACATGG	17350	0.0	20.97006	2
GAGTACT	13825	0.0	20.952751	12-13
TGGTATC	3825	0.0	20.278627	2
ACATGGG	17545	0.0	20.17373	3
CAGAGTA	23120	0.0	20.081707	10-11
ACCGAAC	705	0.0	19.450035	8
AGAGTAC	21625	0.0	18.527424	10-11
GTACTTT	15525	0.0	18.520658	14-15
>>END_MODULE