FastQCFastQC Report
Fri 27 May 2016
SRR2139391_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139391_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4634450
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57650.048.1991961
GTATCAA102450.037.9133951
TCAACGC121350.030.8894824
ATCAACG124450.030.2561763
CAACGCA125900.029.840435
TATCAAC127750.029.7749252
AACGCAG131000.028.646376
ACGCAGA149700.025.0962587
CGCAGAG151450.024.7783078
GTGGTAT30600.021.3174171
GTACATG160550.020.7898431
GCAGAGT182250.020.7070069
TACATGG160850.020.5930632
TGGTATC29500.019.6713332
ACATGGG164750.019.255893
GAGTACT84100.018.6091112-13
CAGAGTA176900.018.38825610-11
AGAGTAC163000.017.89054310-11
GTACTTT92650.016.8004714-15
CATGGGG123300.016.6947674
Produced by FastQC (version 0.11.5)