##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139391_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4634450
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.647612985359643	30.0	18.0	33.0	18.0	34.0
2	31.659027716341743	33.0	32.0	33.0	27.0	34.0
3	32.4432234677254	33.0	33.0	33.0	31.0	34.0
4	32.81520633516383	33.0	33.0	34.0	31.0	34.0
5	33.13929765128548	33.0	33.0	34.0	33.0	34.0
6	36.420161184175036	38.0	37.0	38.0	34.0	38.0
7	37.099893622759986	38.0	38.0	38.0	36.0	38.0
8	36.62312140599208	38.0	37.0	38.0	34.0	38.0
9	37.211646473691594	38.0	38.0	38.0	36.0	38.0
10-11	37.36087755828631	38.0	38.0	38.0	36.5	38.0
12-13	37.429397447377795	38.0	38.0	38.0	37.0	38.0
14-15	37.387100087389015	38.0	38.0	38.0	36.5	38.0
16-17	37.457914207726915	38.0	38.0	38.0	37.0	38.0
18-19	37.47863360269288	38.0	38.0	38.0	37.0	38.0
20-21	37.50637634740688	38.0	38.0	38.0	37.0	38.0
22-23	37.51718316110501	38.0	38.0	38.0	37.0	38.0
24-25	37.537401911068386	38.0	38.0	38.0	37.0	38.0
26-27	37.51133044911978	38.0	38.0	38.0	37.0	38.0
28-29	37.498451136153164	38.0	38.0	38.0	37.0	38.0
30-31	37.50651665269419	38.0	38.0	38.0	37.0	38.0
32-33	37.49332583797913	38.0	38.0	38.0	37.0	38.0
34-35	37.477559604165606	38.0	38.0	38.0	37.0	38.0
36-37	37.457833237569	38.0	38.0	38.0	37.0	38.0
38-39	37.45790795923831	38.0	38.0	38.0	37.0	38.0
40-41	37.44700189410767	38.0	38.0	38.0	37.0	38.0
42-43	37.41744575497647	38.0	38.0	38.0	37.0	38.0
44-45	37.393225347051406	38.0	38.0	38.0	37.0	38.0
46-47	37.37446805805809	38.0	38.0	38.0	37.0	38.0
48-49	37.32780699947952	38.0	38.0	38.0	37.0	38.0
50-51	37.255033155822176	38.0	38.0	38.0	37.0	38.0
52-53	37.2430113047491	38.0	38.0	38.0	37.0	38.0
54-55	37.18000220714305	38.0	38.0	38.0	36.5	38.0
56-57	37.15092795386906	38.0	38.0	38.0	36.0	38.0
58-59	37.15943903319949	38.0	38.0	38.0	36.0	38.0
60-61	37.16858556748333	38.0	38.0	38.0	36.0	38.0
62-63	37.17312534323053	38.0	38.0	38.0	36.0	38.0
64-65	37.17512220215665	38.0	38.0	38.0	36.0	38.0
66-67	37.168672335966434	38.0	38.0	38.0	36.0	38.0
68-69	37.15723730519949	38.0	38.0	38.0	36.0	38.0
70-71	37.14545805355894	38.0	38.0	38.0	36.0	38.0
72-73	37.147118439593996	38.0	38.0	38.0	36.0	38.0
74-75	37.13266926486966	38.0	38.0	38.0	36.0	38.0
76-77	37.12452774356191	38.0	38.0	38.0	36.0	38.0
78-79	37.09642270151788	38.0	38.0	38.0	36.0	38.0
80-81	37.0879850695906	38.0	38.0	38.0	36.0	38.0
82-83	37.03097307247299	38.0	38.0	38.0	36.0	38.0
84-85	37.00859904654628	38.0	38.0	38.0	36.0	38.0
86-87	37.00061907216513	38.0	38.0	38.0	36.0	38.0
88-89	36.980846263236	38.0	38.0	38.0	36.0	38.0
90-91	36.97448278150973	38.0	38.0	38.0	35.0	38.0
92-93	36.97011265440685	38.0	38.0	38.0	35.0	38.0
94-95	36.9700936392822	38.0	38.0	38.0	35.0	38.0
96-97	36.97535974113576	38.0	38.0	38.0	35.0	38.0
98-99	36.95584569599856	38.0	38.0	38.0	35.0	38.0
100	35.399863075712	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	10.0
21	59.0
22	173.0
23	680.0
24	1470.0
25	3096.0
26	5426.0
27	9022.0
28	14095.0
29	20746.0
30	28065.0
31	36968.0
32	48851.0
33	68161.0
34	105925.0
35	206909.0
36	710094.0
37	3374697.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.14005976976772	18.05180765786663	13.258941190432521	22.54919138193313
2	15.057632114731229	20.462114288858654	42.42416330124176	22.056090295168353
3	18.89678386863598	26.28767167625069	29.93431798811078	24.881226467002556
4	13.162079642676044	18.169340482689424	38.338465190044126	30.330114684590402
5	14.293497610288167	37.728425163719535	34.8470260764492	13.131051149543097
6	31.079221914142995	37.28543840153632	18.560498009472536	13.074841674848148
7	27.885034901660394	32.72649397447378	22.47082178036229	16.917649343503545
8	25.296550831274477	36.66191241679164	20.70582269740746	17.335714054526427
9	26.67037080991272	17.520331430914133	20.996989934080634	34.81230782509252
10-11	25.263796135463757	26.78426782034546	28.254949346740176	19.696986697450612
12-13	26.026173548101717	24.35280346103637	28.513858170872485	21.10716481998943
14-15	23.11523481750801	25.659139703740465	26.176946563238356	25.048678915513168
16-17	22.575677804270196	28.76977850661891	26.852927531853833	21.801616157257065
18-19	22.603987528185655	27.938622706038473	28.531368339285134	20.92602142649074
20-21	23.527179739030313	27.099653289050078	28.273578847611176	21.099588124308433
22-23	23.313873133595656	26.901739118238243	28.163088054256953	21.621299693909148
24-25	22.983893069906898	27.121352914798013	28.170716022293075	21.724037993002014
26-27	22.814489822082308	27.275144574043452	28.225899397962472	21.68446620591177
28-29	22.814194331720667	27.31452616013898	28.231413370974025	21.639866137166326
30-31	23.113958388352103	27.076358358689262	28.134448341956347	21.675234911002285
32-33	22.83815823426627	27.31315039599912	28.10857330316011	21.740118066574496
34-35	23.02354817459816	27.280356581580584	28.088899248873346	21.607195994947915
36-37	23.007924945735898	27.22855851101771	28.074041894913066	21.68947464833333
38-39	22.824912027632855	27.390158424508428	28.16483003851964	21.62009950933908
40-41	23.045859427754863	27.241550937637044	28.052430849898393	21.660158784709704
42-43	23.00098140182682	27.350861593533487	27.811290799667738	21.83686620497195
44-45	23.02073352333159	27.399711679914468	27.760703114449374	21.81885168230456
46-47	23.119538756216137	27.435130343172826	27.577748005587345	21.867582895023688
48-49	23.012915354283482	27.513838207560543	27.53160768169316	21.941638756462815
50-51	22.91210207724866	27.736327310217895	27.447065818802507	21.90450479373094
52-53	23.170665928178614	27.57611922036583	27.34886368730946	21.904351164146092
54-55	23.184094450732314	27.441782407094113	27.383008438666117	21.991114703507456
56-57	23.116188029555467	27.57776076639575	27.42007428057174	21.885976923477045
58-59	23.16409971799989	27.595584809951863	27.3583361610027	21.881979311045548
60-61	23.21757354188073	27.544191485013027	27.431614955877713	21.806620017228536
62-63	23.19621444476252	27.706502474496702	27.329487379634188	21.767795701106586
64-65	23.274134459708712	27.630701497863402	27.274456028373763	21.820708014054123
66-67	23.563164771861636	27.679843539011777	27.030734897182285	21.726256791944305
68-69	23.216775454604793	27.680432190104714	27.258948617540646	21.843843737749847
70-71	23.310349069239813	27.567613878536985	27.21821813613083	21.903818916092376
72-73	23.375954267502035	27.548229260143202	27.132658216781763	21.943158255572996
74-75	23.04356050439946	27.483042685671112	27.370024030818907	22.10337277911053
76-77	23.014215746576898	27.451046060436727	27.372075482319975	22.162662710666396
78-79	22.96493138485897	27.463119188896663	27.295335213135285	22.276614213109085
80-81	22.963380598053647	27.45707306129173	27.351137954096473	22.22840838655815
82-83	22.87198546522266	27.378396094209123	27.45840786927311	22.291210571295107
84-85	22.977793821478016	27.39234992759948	27.41110583469087	22.218750416231632
86-87	22.790369429095332	27.422824790804672	27.527008834636664	22.259796945463332
88-89	22.781896120073764	27.388801982429474	27.499270022748647	22.330031874748116
90-91	22.86216776848567	27.368036082648565	27.506872715030127	22.26292343383564
92-93	22.88592471056445	27.39341235180488	27.46050377469091	22.26015916293976
94-95	22.76619558283879	27.45011314455707	27.525360952533855	22.258330320070286
96-97	22.88017444457094	27.570444839494062	27.560680497295465	21.988700218639533
98-99	23.96693706137412	29.533838640447012	27.67507429896017	18.824149999218694
100	30.639284782448716	40.388249487648935	28.972465729902346	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	676.0
1	633.0
2	668.0
3	822.0
4	1103.5
5	1601.0
6	1979.5
7	2332.5
8	2812.0
9	3242.0
10	3467.0
11	3582.5
12	4058.0
13	5227.0
14	7472.0
15	10107.0
16	12407.5
17	14135.0
18	14698.5
19	14115.0
20	13141.5
21	12479.5
22	13024.0
23	15323.5
24	19235.0
25	24997.5
26	32890.0
27	41921.0
28	50943.0
29	60972.0
30	71292.5
31	81757.0
32	93145.5
33	104669.5
34	115452.5
35	126282.5
36	138596.5
37	149741.0
38	158112.0
39	165371.5
40	173800.0
41	181064.0
42	186012.5
43	191239.0
44	196097.0
45	199894.5
46	202027.0
47	203693.0
48	203855.5
49	201214.0
50	196922.0
51	191603.5
52	184141.5
53	175459.0
54	166666.5
55	155928.0
56	143131.0
57	129945.0
58	116457.0
59	100497.0
60	83798.5
61	68244.5
62	54420.0
63	42376.0
64	32320.0
65	24450.5
66	18219.5
67	13997.5
68	10603.0
69	7651.0
70	5612.0
71	4036.5
72	2824.0
73	1907.5
74	1308.5
75	889.5
76	575.0
77	371.0
78	241.5
79	152.0
80	97.5
81	67.0
82	44.0
83	28.5
84	17.0
85	12.5
86	9.5
87	5.5
88	3.5
89	3.0
90	3.0
91	2.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.006796923043726871
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	5.394429345436706E-5
22-23	0.0
24-25	2.3738016887225215E-4
26-27	0.0021690492215541333
28-29	0.0062723350726738005
30-31	0.006507240710508535
32-33	0.0017773286771656939
34-35	4.783738593909192E-4
36-37	0.0
38-39	5.499789523054952E-4
40-41	5.541292269254494E-5
42-43	6.699746291774175E-5
44-45	1.464237684071572E-4
46-47	5.686052999017678E-5
48-49	7.576074984776106E-4
50-51	3.011201205221701E-4
52-53	0.0
54-55	0.007366798919928427
56-57	0.06119400469660426
58-59	0.07403966342124545
60-61	0.04573972405632824
62-63	0.008993150947845209
64-65	8.604658709733885E-5
66-67	0.0
68-69	4.134262815838887E-4
70-71	0.0027081264801366517
72-73	0.012434654167091592
74-75	0.01047625682827454
76-77	0.016846824321498383
78-79	0.006450788187579968
80-81	0.007371400986650047
82-83	0.04206684863358054
84-85	0.025993278162724988
86-87	0.009390510559587938
88-89	0.002386940879915225
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	20.0
20-21	119.0
22-23	326.0
24-25	431.0
26-27	1386.0
28-29	3764.0
30-31	11506.0
32-33	13861.0
34-35	18685.0
36-37	30242.0
38-39	34304.0
40-41	33159.0
42-43	37199.0
44-45	42073.0
46-47	41574.0
48-49	39258.0
50-51	35685.0
52-53	33211.0
54-55	35953.0
56-57	36261.0
58-59	37564.0
60-61	36137.0
62-63	35062.0
64-65	37053.0
66-67	38641.0
68-69	39936.0
70-71	39249.0
72-73	37109.0
74-75	37606.0
76-77	39096.0
78-79	39942.0
80-81	40364.0
82-83	38535.0
84-85	37592.0
86-87	38692.0
88-89	39857.0
90-91	40137.0
92-93	40413.0
94-95	46273.0
96-97	142854.0
98-99	1061206.0
100-101	2242115.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.11223762268364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.41132165659852	80.54456095209774
2	6.274098595898971	11.056497326999915
3	1.3732194937335673	3.629923270198602
4	0.47770885781666156	1.6836798557760986
5	0.1954793368234889	0.8612060888257931
6	0.0946635892468402	0.5004612401960221
7	0.05080572359314864	0.3133624192882328
8	0.03134175444307748	0.22092736920001943
9	0.024180555249823918	0.19175425470188237
>10	0.06541453387528225	0.8557513316997967
>50	0.0011950299226975735	0.07275230487912604
>100	5.708727979247405E-4	0.06912358613686913
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.1577533472148797E-5
2	0.0	2.1577533472148797E-5	0.0	0.0	2.1577533472148797E-5
3	0.0	2.1577533472148797E-5	0.0	0.0	2.1577533472148797E-5
4	0.0	6.47326004164464E-5	0.0	2.1577533472148797E-5	2.1577533472148797E-5
5	0.0	6.47326004164464E-5	0.0	2.1577533472148797E-5	2.1577533472148797E-5
6	0.0	6.47326004164464E-5	0.0	2.1577533472148797E-5	2.1577533472148797E-5
7	0.0	6.47326004164464E-5	0.0	2.1577533472148797E-5	4.3155066944297595E-5
8	0.0	6.47326004164464E-5	0.0	2.1577533472148797E-5	4.3155066944297595E-5
9	0.0	8.631013388859519E-5	0.0	2.1577533472148797E-5	4.3155066944297595E-5
10-11	0.0	8.631013388859519E-5	0.0	2.1577533472148797E-5	4.3155066944297595E-5
12-13	0.0	8.631013388859519E-5	0.0	2.1577533472148797E-5	4.3155066944297595E-5
14-15	0.0	1.834090345132648E-4	0.0	2.1577533472148797E-5	4.3155066944297595E-5
16-17	0.0	1.941978012493392E-4	0.0	2.1577533472148797E-5	6.47326004164464E-5
18-19	0.0	2.1577533472148797E-4	0.0	3.23663002082232E-5	6.47326004164464E-5
20-21	0.0	2.3735286819363677E-4	0.0	5.394383368037199E-5	6.47326004164464E-5
22-23	0.0	2.6971916840185997E-4	0.0	6.47326004164464E-5	9.70989006246696E-5
24-25	0.0	3.0208546861008314E-4	0.0	1.4025396756896718E-4	1.294652008328928E-4
26-27	0.0	3.4524053555438076E-4	0.0	1.941978012493392E-4	1.294652008328928E-4
28-29	0.0	3.4524053555438076E-4	0.0	5.718046370119432E-4	1.294652008328928E-4
30-31	0.0	4.962832698594224E-4	0.0	0.0019635555459655406	1.294652008328928E-4
32-33	0.0	4.962832698594224E-4	0.0	0.005070720365954968	1.510427343050416E-4
34-35	0.0	5.502271035397944E-4	0.0	0.009138085425455016	1.7262026777719038E-4
36-37	0.0	5.933821704840921E-4	0.0	0.015460302732794615	1.7262026777719038E-4
38-39	0.0	6.041709372201664E-4	0.0	0.03157872023648977	1.941978012493392E-4
40-41	0.0	6.689035376366127E-4	0.0	0.04955280561878972	2.15775334721488E-4
42-43	0.0	6.796923043726872E-4	0.0	0.060287628521183745	2.2656410145756238E-4
44-45	0.0	7.228473713169847E-4	0.0	0.0718963415291998	2.3735286819363677E-4
46-47	0.0	7.55213671525208E-4	0.0	0.083817928772562	2.3735286819363677E-4
48-49	0.0	7.55213671525208E-4	0.0	0.09622501051904757	2.589304016657856E-4
50-51	0.0	9.062564058302495E-4	0.0	0.10742375039109278	2.589304016657856E-4
52-53	0.0	9.386227060384728E-4	0.0	0.13263709825329867	3.0208546861008314E-4
54-55	0.0	9.602002395106215E-4	0.0	0.1630937867492367	3.4524053555438076E-4
56-57	0.0	9.70989006246696E-4	0.0	0.20197650206604884	3.4524053555438076E-4
58-59	0.0	9.70989006246696E-4	0.0	0.23462331020940996	3.4524053555438076E-4
60-61	0.0	9.70989006246696E-4	0.0	0.2450128925762496	3.4524053555438076E-4
62-63	0.0	9.925665397188448E-4	0.0	0.2571934102212776	3.668180690265296E-4
64-65	0.0	0.0010572991401352912	0.0	0.2637421916300748	3.668180690265296E-4
66-67	0.0	0.0010788766736074399	0.0	0.27016150783803905	3.668180690265296E-4
68-69	0.0	0.0011004542070795888	0.0	0.27631110487760147	3.668180690265296E-4
70-71	0.0	0.0011112429738156632	0.0	0.2816515444119583	3.668180690265296E-4
72-73	0.0	0.001132820507287812	0.0	0.2864957006764557	4.0997313597082715E-4
74-75	0.0	0.0012191306411764072	0.0	0.28975390823075015	4.0997313597082715E-4
76-77	0.0	0.0012299194079124815	0.0	0.2929905382515725	4.207619027069016E-4
78-79	0.0	0.0012299194079124815	0.0	0.2953317006333006	4.31550669442976E-4
80-81	0.0	0.001240708174648556	0.0	0.2960437592378815	4.423394361790504E-4
82-83	0.0	0.0012514969413846304	0.0	0.2966047751081574	4.962832698594224E-4
84-85	0.0	0.0012514969413846304	0.0	0.2967234515422542	4.962832698594224E-4
86-87	0.0	0.0012514969413846304	0.0	0.2967450290757263	5.394383368037199E-4
88	0.0	0.0012514969413846304	0.0	0.2967558178424624	5.394383368037199E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5765	0.0	48.199196	1
GTATCAA	10245	0.0	37.913395	1
TCAACGC	12135	0.0	30.889482	4
ATCAACG	12445	0.0	30.256176	3
CAACGCA	12590	0.0	29.84043	5
TATCAAC	12775	0.0	29.774925	2
AACGCAG	13100	0.0	28.64637	6
ACGCAGA	14970	0.0	25.096258	7
CGCAGAG	15145	0.0	24.778307	8
GTGGTAT	3060	0.0	21.317417	1
GTACATG	16055	0.0	20.789843	1
GCAGAGT	18225	0.0	20.707006	9
TACATGG	16085	0.0	20.593063	2
TGGTATC	2950	0.0	19.671333	2
ACATGGG	16475	0.0	19.25589	3
GAGTACT	8410	0.0	18.60911	12-13
CAGAGTA	17690	0.0	18.388256	10-11
AGAGTAC	16300	0.0	17.890543	10-11
GTACTTT	9265	0.0	16.80047	14-15
CATGGGG	12330	0.0	16.694767	4
>>END_MODULE