FastQCFastQC Report
Fri 27 May 2016
SRR2139390_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139390_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5860601
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81950.051.463121
GTATCAA152750.038.7482151
TCAACGC182100.031.6855854
ATCAACG183600.031.588473
TATCAAC186000.031.3196092
CAACGCA184950.031.2661425
AACGCAG190450.030.474596
ACGCAGA220050.026.2981837
CGCAGAG224900.025.731068
GTGGTAT36200.024.8476891
TGGTATC37450.023.2252672
GTACATG221050.022.2075961
GCAGAGT263350.022.022579
TACATGG217300.021.9660262
GAGTACT132400.020.95668212-13
ACATGGG223650.020.8099233
CAGAGTA251000.019.8016510-11
GTACTTT147900.018.54558414-15
AGAGTAC235550.018.4797910-11
CATGGGG167100.018.1281934
Produced by FastQC (version 0.11.5)