##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139390_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5860601
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.535146480710768	30.0	18.0	33.0	18.0	34.0
2	31.550585511622444	32.0	32.0	33.0	27.0	34.0
3	32.36754865243343	33.0	33.0	33.0	31.0	34.0
4	32.767222167146336	33.0	33.0	34.0	31.0	34.0
5	33.088534264659884	33.0	33.0	34.0	32.0	34.0
6	36.33709614423503	38.0	37.0	38.0	33.0	38.0
7	37.030030708454646	38.0	38.0	38.0	35.0	38.0
8	36.581218718012025	38.0	37.0	38.0	34.0	38.0
9	37.15427854583515	38.0	38.0	38.0	36.0	38.0
10-11	37.30751769315127	38.0	38.0	38.0	36.5	38.0
12-13	37.387296968348465	38.0	38.0	38.0	37.0	38.0
14-15	37.35265837070294	38.0	38.0	38.0	36.5	38.0
16-17	37.41861619653001	38.0	38.0	38.0	37.0	38.0
18-19	37.43869451273001	38.0	38.0	38.0	37.0	38.0
20-21	37.466873847427024	38.0	38.0	38.0	37.0	38.0
22-23	37.48054156319648	38.0	38.0	38.0	37.0	38.0
24-25	37.50069612697135	38.0	38.0	38.0	37.0	38.0
26-27	37.46954104048781	38.0	38.0	38.0	37.0	38.0
28-29	37.45408844951127	38.0	38.0	38.0	37.0	38.0
30-31	37.46256129763083	38.0	38.0	38.0	37.0	38.0
32-33	37.44396685057599	38.0	38.0	38.0	37.0	38.0
34-35	37.42604002367389	38.0	38.0	38.0	37.0	38.0
36-37	37.40201238407459	38.0	38.0	38.0	37.0	38.0
38-39	37.40163638946182	38.0	38.0	38.0	37.0	38.0
40-41	37.388268796811886	38.0	38.0	38.0	37.0	38.0
42-43	37.356875771448316	38.0	38.0	38.0	37.0	38.0
44-45	37.32766050860763	38.0	38.0	38.0	37.0	38.0
46-47	37.29758958177764	38.0	38.0	38.0	37.0	38.0
48-49	37.240547967737434	38.0	38.0	38.0	37.0	38.0
50-51	37.16813865898562	38.0	38.0	38.0	37.0	38.0
52-53	37.15628270236217	38.0	38.0	38.0	36.0	38.0
54-55	37.10017796309106	38.0	38.0	38.0	36.0	38.0
56-57	37.070832050404135	38.0	38.0	38.0	36.0	38.0
58-59	37.076839877248545	38.0	38.0	38.0	36.0	38.0
60-61	37.08965219157647	38.0	38.0	38.0	36.0	38.0
62-63	37.099509432903176	38.0	38.0	38.0	36.0	38.0
64-65	37.10572707442493	38.0	38.0	38.0	36.0	38.0
66-67	37.102992914732994	38.0	38.0	38.0	36.0	38.0
68-69	37.09434197335502	38.0	38.0	38.0	36.0	38.0
70-71	37.08509391959024	38.0	38.0	38.0	36.0	38.0
72-73	37.08813921864581	38.0	38.0	38.0	36.0	38.0
74-75	37.071087805822245	38.0	38.0	38.0	36.0	38.0
76-77	37.064163426579704	38.0	38.0	38.0	36.0	38.0
78-79	37.036216365940064	38.0	38.0	38.0	36.0	38.0
80-81	37.031058667886185	38.0	38.0	38.0	36.0	38.0
82-83	36.97596047529255	38.0	38.0	38.0	36.0	38.0
84-85	36.9545781627568	38.0	38.0	38.0	36.0	38.0
86-87	36.94796769075897	38.0	38.0	38.0	35.0	38.0
88-89	36.923564002547536	38.0	38.0	38.0	35.0	38.0
90-91	36.91938061199097	38.0	38.0	38.0	35.0	38.0
92-93	36.91596882432354	38.0	38.0	38.0	35.0	38.0
94-95	36.918025727538236	38.0	38.0	38.0	35.0	38.0
96-97	36.93262332092878	38.0	38.0	38.0	35.0	38.0
98-99	36.91966016108992	38.0	38.0	38.0	35.0	38.0
100	35.324831068596296	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	16.0
21	78.0
22	285.0
23	855.0
24	2062.0
25	4201.0
26	7635.0
27	12636.0
28	20462.0
29	30663.0
30	41824.0
31	55082.0
32	71365.0
33	97570.0
34	148893.0
35	281909.0
36	919571.0
37	4165490.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.25704769869166	17.770549470950165	13.29483102500921	22.677571805348972
2	15.054942335930214	20.117708590184524	42.32200585812685	22.505343215758415
3	18.731440683301933	25.997623793191178	29.787832340062053	25.48310318344484
4	13.019210828377501	17.850319446759812	37.999037982623285	31.131431742239407
5	14.193100673463352	37.389117600737535	34.68765404776746	13.730127678031657
6	30.908553576672425	36.89428780427127	18.48552733755463	13.711631281501676
7	27.665234333475354	32.561660484991215	22.33185640858335	17.441248772950075
8	25.253160895955894	36.288855016746574	20.534822281878597	17.923161805418932
9	26.3852120285957	17.18511463244128	20.797082074005722	35.6325912649573
10-11	25.09518221765993	26.496582858993474	28.04071630196289	20.367518621383713
12-13	25.884666094825427	24.03383202507729	28.33972147225174	21.741780407845543
14-15	23.02142732460374	25.209206359552542	26.17110429459368	25.59826202125004
16-17	22.38877036672519	28.376637481377763	26.795280893546586	22.439311258350468
18-19	22.44580376654203	27.419721970494155	28.65215529943089	21.48231896353292
20-21	23.424462744400117	26.573084413515218	28.437577131219033	21.564875710865632
22-23	23.231140748208347	26.339424128805454	28.3463697617882	22.083065361198
24-25	22.80482756196312	26.539167627130155	28.478769177842118	22.17723563306461
26-27	22.654528694702652	26.71010344594827	28.457880270720953	22.17748758862812
28-29	22.617876775224865	26.78025339694723	28.45454908682231	22.14732074100559
30-31	22.925910222392428	26.579297398583034	28.298889405585797	22.195902973438738
32-33	22.700910725645347	26.839561508624644	28.240319271165816	22.219208494564192
34-35	22.827675823823913	26.795846073099934	28.26400020289059	22.112477900185567
36-37	22.791309005739908	26.711300976884395	28.275131328272916	22.22225868910278
38-39	22.622310705419476	26.88907606636959	28.34006291064886	22.148550317562073
40-41	22.901641795895998	26.71292397261708	28.187744655395804	22.197689576091122
42-43	22.803672760162083	26.803980953443396	28.019111513719718	22.3732347726748
44-45	22.84655472968065	26.89765093263415	27.93329889154897	22.32249544613623
46-47	22.94455855662348	26.93515257754473	27.624379154603258	22.495909711228535
48-49	22.80686283075483	26.99883207363679	27.686668392290798	22.50763670331758
50-51	22.780461974790974	27.199771534330946	27.56128001186264	22.458486479015438
52-53	23.056199522095362	27.06012050697796	27.376702568730515	22.506977402196167
54-55	22.973809733156738	26.98029670547964	27.440339533267366	22.605554028096257
56-57	22.97477324582459	27.127109304039543	27.441396638937032	22.456720811198835
58-59	22.94933945333154	27.150078932057788	27.413037339695904	22.487544274914768
60-61	23.019528521956698	27.045659579238784	27.5137847912399	22.42102710756462
62-63	22.97952468609796	27.108853838985247	27.463476103782398	22.448145371134398
64-65	22.975052186984744	27.099974043771475	27.431069012762478	22.493904756481307
66-67	23.14954172133688	27.08779752960085	27.268004547045276	22.494656202016987
68-69	22.95648694620265	27.092151922742453	27.4618310843899	22.489530046665003
70-71	23.03737464868246	26.872225868365803	27.479323767087593	22.61107571586415
72-73	23.12777850995677	26.92562458512193	27.35048798440452	22.596108920516777
74-75	22.814383697661274	27.00134936274435	27.471039620345334	22.71322731924904
76-77	22.98047086482998	27.013134364817333	27.353486249907217	22.65290852044548
78-79	22.922040898701713	27.028179109138506	27.308919577957024	22.740860414202757
80-81	22.92985221143824	27.050186281185333	27.274382270783747	22.74557923659268
82-83	22.929000948349394	27.04007968254136	27.383911967745522	22.647007401363716
84-85	23.095417122774272	26.95603170818356	27.28426872432038	22.66428244472179
86-87	22.844300245107434	27.022228135335762	27.3806182512977	22.7528533682591
88-89	22.915665810129823	27.06928043487462	27.310936836625743	22.704116918369813
90-91	23.01727876867688	27.053158844148427	27.31601274381678	22.613549643357906
92-93	23.029059284238492	27.06684660629551	27.239579734726494	22.664514374739507
94-95	22.87685025492683	27.130637815714582	27.280714752964098	22.71179717639449
96-97	23.02800017720979	27.16817266369554	27.321010384287675	22.482816774806995
98-99	24.086307129126894	29.169072399979356	27.4481453853796	19.29647508551415
100	23.10359396286785	29.91198426189911	21.398213685710797	25.586208089522245
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	780.0
1	750.5
2	1071.0
3	1546.5
4	2073.5
5	2969.0
6	3704.0
7	4627.5
8	5891.0
9	6735.5
10	6884.5
11	6975.5
12	7646.0
13	9293.0
14	12724.5
15	16681.5
16	19862.0
17	22142.5
18	22387.5
19	20707.0
20	18582.5
21	16988.0
22	17077.5
23	19286.0
24	23634.0
25	30564.0
26	40499.0
27	51528.5
28	61146.0
29	72199.0
30	83278.5
31	93863.0
32	106453.5
33	119999.0
34	133821.5
35	146721.5
36	161954.5
37	175931.5
38	184545.0
39	191915.0
40	202225.0
41	211410.0
42	217247.5
43	224942.5
44	232521.0
45	239766.0
46	244733.5
47	247279.5
48	250236.0
49	250058.5
50	248703.5
51	246188.0
52	240591.0
53	233110.0
54	224497.0
55	213905.0
56	199757.5
57	183604.5
58	166092.0
59	147089.0
60	125110.0
61	102471.5
62	81447.0
63	63214.5
64	48276.5
65	35786.5
66	26364.5
67	19858.5
68	15122.0
69	11191.5
70	8100.5
71	5850.5
72	4171.0
73	2785.0
74	1859.0
75	1233.0
76	777.5
77	469.5
78	294.0
79	199.0
80	135.5
81	88.5
82	63.5
83	43.5
84	30.5
85	21.0
86	13.5
87	9.5
88	7.5
89	5.5
90	4.0
91	2.5
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.006245093293332886
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	1.1091099733728289E-4
22-23	0.0
24-25	4.948765769284786E-4
26-27	0.0030634415709533173
28-29	0.006880673247413132
30-31	0.007145863485287214
32-33	0.00258802520736552
34-35	8.425046700979533E-4
36-37	6.045821279477157E-5
38-39	6.174151956314831E-4
40-41	9.637051132441117E-5
42-43	1.5003659569082543E-4
44-45	1.1571571639288476E-4
46-47	5.3923030805059165E-5
48-49	9.61695051035614E-4
50-51	4.210487390459823E-4
52-53	0.0
54-55	0.007898744239219749
56-57	0.06463375503722377
58-59	0.07643834612529166
60-61	0.04740268716490566
62-63	0.009278811818758887
64-65	7.774229381941962E-5
66-67	9.805582749705049E-6
68-69	4.1575740357521665E-4
70-71	0.00301855592284651
72-73	0.012309855613465691
74-75	0.011231546736726538
76-77	0.016405754184649436
78-79	0.007038260524016143
80-81	0.0077386143539132585
82-83	0.04284279131641758
84-85	0.026222986286939573
86-87	0.009113619516103517
88-89	0.00226892028628538
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	21.0
20-21	151.0
22-23	316.0
24-25	452.0
26-27	1696.0
28-29	4968.0
30-31	14437.0
32-33	17424.0
34-35	23394.0
36-37	36981.0
38-39	43342.0
40-41	41341.0
42-43	45845.0
44-45	53125.0
46-47	53363.0
48-49	49826.0
50-51	45317.0
52-53	42956.0
54-55	46110.0
56-57	47123.0
58-59	46853.0
60-61	45457.0
62-63	43935.0
64-65	45172.0
66-67	47878.0
68-69	48631.0
70-71	47806.0
72-73	45135.0
74-75	45626.0
76-77	47219.0
78-79	48179.0
80-81	48260.0
82-83	45809.0
84-85	44684.0
86-87	46094.0
88-89	48186.0
90-91	48903.0
92-93	48501.0
94-95	57000.0
96-97	179813.0
98-99	397818.0
100-101	3815454.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.42609568953837
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.50049048376145	78.21604050499724
2	6.7428666617581845	11.655192786618223
3	1.5763359142825888	4.08709675699929
4	0.5580679359021847	1.9292653131818154
5	0.24116682993010435	1.0421553760340916
6	0.13519489735230636	0.7010620281184649
7	0.07448008053659165	0.45059157971939956
8	0.04825549765605026	0.33364274063744753
9	0.02802236756727721	0.2179677438735225
>10	0.09292502091706802	1.2179609060042584
>50	0.001851815253910731	0.09833019775407474
>100	3.4249508230274736E-4	0.05069406606215978
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.7063096429871272E-5	0.0	0.0	0.0
3	0.0	1.7063096429871272E-5	0.0	0.0	0.0
4	0.0	8.531548214935635E-5	0.0	0.0	1.7063096429871272E-5
5	0.0	1.3650477143897018E-4	0.0	0.0	1.7063096429871272E-5
6	0.0	1.3650477143897018E-4	0.0	0.0	3.4126192859742545E-5
7	0.0	1.3650477143897018E-4	0.0	0.0	3.4126192859742545E-5
8	0.0	1.5356786786884145E-4	0.0	0.0	5.118928928961381E-5
9	0.0	2.7300954287794036E-4	0.0	0.0	5.118928928961381E-5
10-11	0.0	2.900726393078116E-4	0.0	1.7063096429871272E-5	5.118928928961381E-5
12-13	0.0	2.900726393078116E-4	0.0	1.7063096429871272E-5	5.972083750454945E-5
14-15	0.0	4.265774107467818E-4	0.0	2.559464464480691E-5	1.2797322322403453E-4
16-17	0.0	4.43640507176653E-4	0.0	3.4126192859742545E-5	1.3650477143897018E-4
18-19	0.0	4.43640507176653E-4	0.0	3.4126192859742545E-5	1.3650477143897018E-4
20-21	0.0	4.43640507176653E-4	0.0	4.265774107467818E-5	1.450363196539058E-4
22-23	0.0	4.7776670003639557E-4	0.0	7.678393393442071E-5	1.6209941608377707E-4
24-25	0.0	4.862982482513312E-4	0.0	1.1944167500909889E-4	1.706309642987127E-4
26-27	0.0	4.948297964662669E-4	0.0	1.7063096429871272E-4	1.8769406072858397E-4
28-29	0.0	4.948297964662669E-4	0.0	5.289559893260094E-4	1.8769406072858397E-4
30-31	0.0	5.801452786156232E-4	0.0	0.0018428144144260973	1.8769406072858397E-4
32-33	0.0	5.801452786156232E-4	0.0	0.003975701468160006	2.0475715715845524E-4
34-35	0.0	7.251815982695291E-4	0.0	0.007029995729106963	2.218202535883265E-4
36-37	0.0	8.702179179234348E-4	0.0	0.011253112095500103	2.218202535883265E-4
38-39	0.0	8.87281014353306E-4	0.0	0.01757498932276741	2.3035180180326214E-4
40-41	0.0	0.0010408488822221476	0.0	0.024263723123276945	2.3888335001819779E-4
42-43	0.0	0.0010664435268669544	0.0	0.030705042025553353	2.4741489823313343E-4
44-45	0.0	0.001194416750090989	0.0	0.037777695495734995	2.5594644644806906E-4
46-47	0.0	0.0012967953286702166	0.0	0.04453468168196402	2.5594644644806906E-4
48-49	0.0	0.0013053268768851523	0.0	0.052025381014677505	2.5594644644806906E-4
50-51	0.0	0.0015015524858286717	0.0	0.05870558326697211	2.5594644644806906E-4
52-53	0.0	0.0015015524858286717	0.0	0.06568438970678946	2.81541091092876E-4
54-55	0.0	0.0015015524858286717	0.0	0.07224515028407497	3.156672839526185E-4
56-57	0.0	0.0015356786786884145	0.0	0.07882297395779034	3.3273038038248977E-4
58-59	0.0	0.0015356786786884145	0.0	0.08571646491545834	3.412619285974254E-4
60-61	0.0	0.0015783364197630926	0.0	0.0924137302641828	3.583250250272967E-4
62-63	0.0	0.0016039310644078994	0.0	0.09869294975037543	3.583250250272967E-4
64-65	0.0	0.001825751317996226	0.0	0.10463090730797063	3.583250250272967E-4
66-67	0.0	0.001825751317996226	0.0	0.11115754169239639	3.583250250272967E-4
68-69	0.0	0.0018684090590709041	0.0	0.1170954992499916	3.583250250272967E-4
70-71	0.0	0.0018769406072858398	0.0	0.12276897881292378	3.7538812145716794E-4
72-73	0.0	0.0018940037037157111	0.0	0.12729923091505463	4.265774107467818E-4
74-75	0.0	0.0019537245412202604	0.0	0.13067772400816913	4.265774107467818E-4
76-77	0.0	0.00201344537872481	0.0	0.13369789207625632	4.265774107467818E-4
78-79	0.0	0.0020475715715845525	0.0	0.1357198690031961	4.351089589617174E-4
80-81	0.0	0.0020475715715845525	0.0	0.13629148273359676	4.6923515182145995E-4
82-83	0.0	0.0020475715715845525	0.0	0.13678631253006304	5.118928928961381E-4
84-85	0.0	0.0020731662162293595	0.0	0.1369910696872215	5.118928928961381E-4
86-87	0.0	0.0020987608608741665	0.0	0.13706785362115592	5.289559893260094E-4
88	0.0	0.0021158239573040374	0.0	0.13706785362115592	5.460190857558807E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8195	0.0	51.46312	1
GTATCAA	15275	0.0	38.748215	1
TCAACGC	18210	0.0	31.685585	4
ATCAACG	18360	0.0	31.58847	3
TATCAAC	18600	0.0	31.319609	2
CAACGCA	18495	0.0	31.266142	5
AACGCAG	19045	0.0	30.47459	6
ACGCAGA	22005	0.0	26.298183	7
CGCAGAG	22490	0.0	25.73106	8
GTGGTAT	3620	0.0	24.847689	1
TGGTATC	3745	0.0	23.225267	2
GTACATG	22105	0.0	22.207596	1
GCAGAGT	26335	0.0	22.02257	9
TACATGG	21730	0.0	21.966026	2
GAGTACT	13240	0.0	20.956682	12-13
ACATGGG	22365	0.0	20.809923	3
CAGAGTA	25100	0.0	19.80165	10-11
GTACTTT	14790	0.0	18.545584	14-15
AGAGTAC	23555	0.0	18.47979	10-11
CATGGGG	16710	0.0	18.128193	4
>>END_MODULE