FastQCFastQC Report
Fri 27 May 2016
SRR2139389_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139389_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5600203
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69450.051.0090371
GTATCAA130450.038.9973831
ATCAACG159100.031.1298583
CAACGCA161600.030.5184045
TCAACGC162000.030.494874
TATCAAC165600.030.3143182
AACGCAG176200.028.1325636
ACGCAGA186250.026.4793247
CGCAGAG200050.024.6317278
CTCGATA7150.021.35133694
GCAGAGT231800.021.275999
GTGGTAT37050.020.6186471
TACATGG183500.020.6094152
GAGTACT122400.020.5233412-13
GTACATG191400.020.350871
ACATGGG182550.020.1642953
CAGAGTA224450.018.81237610-11
AGAGTAC208950.018.76159710-11
CATGGGG134300.018.6266844
TGGTATC38000.018.5567822
Produced by FastQC (version 0.11.5)