##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139389_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5600203
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.36602476731647	30.0	18.0	33.0	18.0	34.0
2	31.52327192424989	32.0	32.0	33.0	27.0	34.0
3	32.3710199433842	33.0	33.0	33.0	31.0	34.0
4	32.76261271243203	33.0	33.0	34.0	31.0	34.0
5	33.099504785808655	33.0	33.0	34.0	33.0	34.0
6	36.362183299426825	38.0	37.0	38.0	34.0	38.0
7	37.06203417983241	38.0	38.0	38.0	35.0	38.0
8	36.572745309411104	38.0	37.0	38.0	34.0	38.0
9	37.17574595063786	38.0	38.0	38.0	36.0	38.0
10-11	37.33118585165573	38.0	38.0	38.0	36.5	38.0
12-13	37.40255478238914	38.0	38.0	38.0	37.0	38.0
14-15	37.36106646491207	38.0	38.0	38.0	36.5	38.0
16-17	37.43491432364148	38.0	38.0	38.0	37.0	38.0
18-19	37.45729601230527	38.0	38.0	38.0	37.0	38.0
20-21	37.4861249291185	38.0	38.0	38.0	37.0	38.0
22-23	37.498550588941725	38.0	38.0	38.0	37.0	38.0
24-25	37.518550739192506	38.0	38.0	38.0	37.0	38.0
26-27	37.48995427733348	38.0	38.0	38.0	37.0	38.0
28-29	37.47607023146676	38.0	38.0	38.0	37.0	38.0
30-31	37.48281511649034	38.0	38.0	38.0	37.0	38.0
32-33	37.46536651530543	38.0	38.0	38.0	37.0	38.0
34-35	37.44700905437409	38.0	38.0	38.0	37.0	38.0
36-37	37.42250585048534	38.0	38.0	38.0	37.0	38.0
38-39	37.421345293957316	38.0	38.0	38.0	37.0	38.0
40-41	37.40918421207613	38.0	38.0	38.0	37.0	38.0
42-43	37.37735819068066	38.0	38.0	38.0	37.0	38.0
44-45	37.34553482205774	38.0	38.0	38.0	37.0	38.0
46-47	37.317332995576436	38.0	38.0	38.0	37.0	38.0
48-49	37.26308832898678	38.0	38.0	38.0	37.0	38.0
50-51	37.186359784051206	38.0	38.0	38.0	37.0	38.0
52-53	37.17546310491939	38.0	38.0	38.0	36.5	38.0
54-55	37.11644955456714	38.0	38.0	38.0	36.5	38.0
56-57	37.0818497168337	38.0	38.0	38.0	36.0	38.0
58-59	37.08615572294498	38.0	38.0	38.0	36.0	38.0
60-61	37.09895231811183	38.0	38.0	38.0	36.0	38.0
62-63	37.10759513890746	38.0	38.0	38.0	36.0	38.0
64-65	37.110588950813494	38.0	38.0	38.0	36.0	38.0
66-67	37.10727257231091	38.0	38.0	38.0	36.0	38.0
68-69	37.09913654772693	38.0	38.0	38.0	36.0	38.0
70-71	37.09039135047148	38.0	38.0	38.0	36.0	38.0
72-73	37.09311610157574	38.0	38.0	38.0	36.0	38.0
74-75	37.082441472166565	38.0	38.0	38.0	36.0	38.0
76-77	37.07446602106117	38.0	38.0	38.0	36.0	38.0
78-79	37.047693101456574	38.0	38.0	38.0	36.0	38.0
80-81	37.039575387287925	38.0	38.0	38.0	36.0	38.0
82-83	36.984764028595336	38.0	38.0	38.0	36.0	38.0
84-85	36.96312818136148	38.0	38.0	38.0	35.5	38.0
86-87	36.955273859045036	38.0	38.0	38.0	35.0	38.0
88-89	36.93341011020006	38.0	38.0	38.0	35.0	38.0
90-91	36.927631395540544	38.0	38.0	38.0	35.0	38.0
92-93	36.92423469424776	38.0	38.0	38.0	35.0	38.0
94-95	36.92787246878415	38.0	38.0	38.0	35.0	38.0
96-97	36.94003284512991	38.0	38.0	38.0	35.0	38.0
98-99	36.92516134690711	38.0	38.0	38.0	35.0	38.0
100	35.31478110556819	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	9.0
21	57.0
22	248.0
23	664.0
24	1696.0
25	3559.0
26	6521.0
27	11739.0
28	19700.0
29	28968.0
30	38392.0
31	48715.0
32	64369.0
33	88360.0
34	136330.0
35	264593.0
36	905099.0
37	3981183.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.23164553142091	17.894226334295382	13.05327681871532	22.820851315568383
2	15.200039286415828	20.04091144484723	42.30613046661548	22.452918802121467
3	18.487561968735776	26.284457902686743	29.966020874600442	25.261959253977047
4	12.868051390279959	18.00034748740358	38.514425280655004	30.617175841661453
5	14.02683081309731	37.4254826119696	35.06437177366607	13.483314801267026
6	30.79145523831904	37.354592324599665	18.517918011186378	13.336034425894919
7	27.54032309185935	32.40950372691847	22.796262921183395	17.253910260038786
8	25.267744758538218	35.89923436703991	20.763425897239795	18.069594977182078
9	26.514895977877945	16.8844057974327	21.038165937913323	35.56253228677603
10-11	25.024664284491116	26.485780961868706	28.356847064293923	20.13270768934626
12-13	25.899070087280762	23.9051066541695	28.371248327962395	21.82457493058734
14-15	23.050289427008273	25.054966757455045	26.35497320364994	25.53977061188675
16-17	22.420937241025012	28.13437298612211	27.016288516684128	22.42840125616875
18-19	22.342324019325726	27.275636258185642	28.860328813080528	21.521710909408107
20-21	23.39413610925625	26.606046921375665	28.523207971635788	21.476608997732292
22-23	23.242974854781018	26.32807802726826	28.321795517171367	22.10715160077936
24-25	22.78553033318636	26.512248335121836	28.51444168296677	22.18777964872504
26-27	22.69928629711719	26.77859761165522	28.464223811165713	22.05789228006187
28-29	22.63282505697895	26.829808277337165	28.51316881582956	22.024197849854325
30-31	22.9819670710037	26.60711603480786	28.290817424254012	22.12009946993443
32-33	22.792973871115635	26.777462113821837	28.26647384164828	22.16309017341424
34-35	22.79479304614973	26.90264589820926	28.258011776609322	22.04454927903169
36-37	22.854872694504984	26.694751745284446	28.232414774634023	22.217960785576548
38-39	22.727686632948917	26.90392447132085	28.356925639230912	22.01146325649932
40-41	22.92145890217433	26.646143811827994	28.41986150565766	22.01253578034001
42-43	22.93793110863006	26.707366738299136	28.052046586007997	22.302655567062803
44-45	22.920687573557743	27.07381475011119	27.835703791001087	22.169793885329984
46-47	23.000691174679172	26.890024158957317	27.572611172439572	22.536673493923942
48-49	22.99089037145283	26.80773059240063	27.690374893494806	22.51100414265173
50-51	22.760411197113932	27.045213768138325	27.67572128600639	22.51865374874136
52-53	23.050980079729392	27.099743354772215	27.34523686968595	22.504039695812438
54-55	23.126513206541414	26.905102220922355	27.359462201628787	22.60892237090744
56-57	23.16488027601513	27.000361024738336	27.44622061907998	22.388538080166555
58-59	23.00872753447828	26.909468438788075	27.687235954501823	22.394568072231827
60-61	23.15293120642526	27.00515153147018	27.423533789438693	22.418383472665866
62-63	23.064087342767166	27.101091907156434	27.37552928448745	22.45929146558895
64-65	22.964284039077203	27.045504333120107	27.446709039992122	22.543502587810565
66-67	23.23452376712955	27.093005360627743	27.15579349098465	22.516677381258067
68-69	23.170703991323293	26.999623108677905	27.452345133125128	22.377327766873673
70-71	23.22890169119556	26.632945488739594	27.443389567555776	22.694763252509066
72-73	23.21265478266533	26.78169203755001	27.395846610961712	22.60980656882295
74-75	22.86311745051179	26.995123209471412	27.48227031198128	22.65948902803552
76-77	22.997388662686117	26.97771141700021	27.344885126283025	22.680014794030644
78-79	23.11336016985296	26.9978227079284	27.235795218520636	22.653021903698004
80-81	22.92633356010332	26.96908448947135	27.263399323998065	22.84118262642726
82-83	23.25087030144761	26.847927115665904	27.400509372219567	22.50069321066692
84-85	23.285096350841226	26.83431839987446	27.166041335628176	22.714543913656136
86-87	22.88799096285887	27.03247250025832	27.36197189844326	22.717564638439555
88-89	23.116854051988387	26.801572837943105	27.33925511065006	22.74231799941845
90-91	23.112871430981073	26.846467300826554	27.302364929629476	22.738296338562893
92-93	23.199490603496695	26.98506350936947	27.197484254061504	22.61796163307233
94-95	22.926456606163498	27.141309489333242	27.207609944606098	22.724623959897166
96-97	22.993008882434395	27.11790556702577	27.397557692400476	22.491527858139364
98-99	24.192846763230673	29.03698495288989	27.450321768420487	19.319846515458945
100	31.312243232338055	40.305989105714914	28.381767661947034	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	581.0
1	604.5
2	906.0
3	1373.0
4	2000.5
5	3055.0
6	3860.0
7	4540.0
8	5296.0
9	5674.0
10	5779.0
11	6018.0
12	6867.5
13	8620.5
14	12027.5
15	16267.0
16	19897.5
17	22438.5
18	22751.0
19	20926.0
20	18604.0
21	16701.5
22	16602.0
23	19101.0
24	23502.5
25	30108.5
26	40323.0
27	51505.5
28	60022.5
29	69809.5
30	79156.5
31	87646.5
32	100377.0
33	115134.0
34	130008.5
35	145568.5
36	163574.0
37	178074.5
38	184773.5
39	188404.0
40	196005.5
41	203560.0
42	208295.5
43	216234.5
44	225910.0
45	233845.5
46	235691.5
47	240862.5
48	249326.0
49	253510.0
50	254465.0
51	253400.0
52	246932.5
53	234356.0
54	221012.5
55	205903.0
56	190607.5
57	176106.0
58	159630.5
59	137534.5
60	112368.0
61	87838.5
62	66814.0
63	50524.5
64	37432.0
65	27456.5
66	20166.5
67	15141.5
68	11574.5
69	8657.5
70	6315.5
71	4592.0
72	3253.5
73	2326.0
74	1619.5
75	1044.0
76	669.0
77	409.0
78	272.5
79	187.0
80	126.0
81	80.5
82	54.5
83	37.0
84	22.5
85	12.0
86	8.5
87	7.0
88	5.0
89	3.0
90	1.5
91	1.5
92	2.0
93	2.0
94	2.5
95	2.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.005410518154431188
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	9.821146213474934E-5
22-23	8.928584980887918E-6
24-25	2.946599316335384E-4
26-27	0.0025450787018705527
28-29	0.006539531737365973
30-31	0.006762796781445462
32-33	0.00212590983558913
34-35	8.190760102537516E-4
36-37	9.045494131826137E-5
38-39	4.1929007809277704E-4
40-41	1.0110676055869391E-4
42-43	1.019524354814866E-4
44-45	1.1229283726100925E-4
46-47	1.891555961777707E-5
48-49	8.123717527828511E-4
50-51	3.2817208726520494E-4
52-53	0.0
54-55	0.007615819166845106
56-57	0.06407159348146702
58-59	0.07625546201040061
60-61	0.04723952320154663
62-63	0.00930400050574545
64-65	5.1544850720957824E-5
66-67	1.0412624182257577E-5
68-69	5.366095556273139E-4
70-71	0.003264811289653632
72-73	0.012927559138210008
74-75	0.011593002255425101
76-77	0.017211390539944027
78-79	0.007176152016409985
80-81	0.00818907657953265
82-83	0.04403055296066612
84-85	0.02774103247329252
86-87	0.009473674201619731
88-89	0.0024631434566418134
90-91	1.1870168841281598E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	20.0
20-21	119.0
22-23	320.0
24-25	474.0
26-27	1631.0
28-29	4660.0
30-31	14904.0
32-33	17776.0
34-35	23840.0
36-37	38953.0
38-39	46948.0
40-41	44263.0
42-43	49423.0
44-45	55765.0
46-47	55932.0
48-49	52891.0
50-51	47416.0
52-53	44958.0
54-55	48377.0
56-57	49740.0
58-59	47949.0
60-61	46785.0
62-63	45242.0
64-65	47518.0
66-67	49869.0
68-69	50204.0
70-71	49670.0
72-73	46631.0
74-75	47644.0
76-77	49570.0
78-79	50797.0
80-81	50802.0
82-83	47437.0
84-85	47667.0
86-87	48789.0
88-89	50205.0
90-91	50954.0
92-93	49861.0
94-95	58119.0
96-97	170265.0
98-99	1278663.0
100-101	2617152.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.22799712363297
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.98284612578931	74.05864061409868
2	7.401525858877597	12.320283457863194
3	1.8980938894450141	4.739236583133449
4	0.7725380732250208	2.5718718614507594
5	0.359208395692646	1.494809766174618
6	0.18401554853281146	0.9189147326395546
7	0.12157995514192267	0.7083199309790357
8	0.06963624838034493	0.4636548383919952
9	0.05198810732150295	0.3894179441955453
>10	0.15518443401818074	1.9100824957854856
>50	0.0019457976921649028	0.10582037576451872
>100	0.00129174626274388	0.18280873509890574
>500	7.290981056862645E-5	0.03810790351870019
>1k	7.290981021438261E-5	0.09803076090551133
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.7856495559178836E-5	0.0
5	0.0	0.0	0.0	1.7856495559178836E-5	0.0
6	0.0	0.0	0.0	1.7856495559178836E-5	0.0
7	0.0	0.0	0.0	3.571299111835767E-5	0.0
8	0.0	0.0	0.0	5.356948667753651E-5	0.0
9	0.0	0.0	0.0	1.6070846003260952E-4	0.0
10-11	0.0	0.0	0.0	2.2320619448973545E-4	0.0
12-13	0.0	0.0	0.0	3.124886722856296E-4	0.0
14-15	0.0	3.571299111835767E-5	0.0	3.4820166340398737E-4	0.0
16-17	0.0	3.571299111835767E-5	0.0	3.5712991118357675E-4	0.0
18-19	0.0	3.571299111835767E-5	0.0	3.928429023019344E-4	0.0
20-21	0.0	3.571299111835767E-5	0.0	4.2855589342029207E-4	0.0
22-23	0.0	3.571299111835767E-5	0.0	4.999818756570074E-4	0.0
24-25	0.0	3.571299111835767E-5	0.0	5.624796101141333E-4	0.0
26-27	0.0	3.571299111835767E-5	0.0	6.249773445712592E-4	0.0
28-29	0.0	3.571299111835767E-5	0.0	8.928247779589418E-4	0.0
30-31	0.0	8.928247779589419E-5	0.0	0.001982071007068851	0.0
32-33	0.0	8.928247779589419E-5	0.0	0.0041605634652886695	0.0
34-35	0.0	1.517802122530201E-4	0.0	0.006874750790283852	0.0
36-37	0.0	2.1427794671014604E-4	0.0	0.011115668485588825	0.0
38-39	0.0	2.1427794671014604E-4	0.0	0.018017204019211447	0.0
40-41	0.0	2.499909378285037E-4	0.0	0.025436577924050255	0.0
42-43	0.0	2.499909378285037E-4	0.0	0.032025624785387244	0.0
44-45	0.0	2.499909378285037E-4	0.0	0.039168223009058784	0.0
46-47	0.0	2.499909378285037E-4	0.0	0.0464090319583058	0.0
48-49	0.0	2.499909378285037E-4	0.0	0.053756979880907885	0.0
50-51	0.0	2.857039289468614E-4	0.0	0.0610067170779345	0.0
52-53	0.0	2.857039289468614E-4	0.0	0.06767611816928779	0.0
54-55	0.0	2.946321767264508E-4	0.0	0.07451515596845329	0.0
56-57	0.0	3.2141692006521904E-4	0.0	0.0814166915020759	0.0
58-59	0.0	3.2141692006521904E-4	0.0	0.08845215075239236	0.0
60-61	0.0	3.2141692006521904E-4	0.0	0.0947554936847825	0.0
62-63	0.0	3.392734156243979E-4	0.0	0.10118383208608689	0.0
64-65	0.0	3.749864067427556E-4	0.0	0.10761217048739126	0.0
66-67	0.0	3.749864067427556E-4	0.0	0.11412979136649154	0.0
68-69	0.0	3.83914654522345E-4	0.0	0.11987065468876754	0.0
70-71	0.0	3.928429023019344E-4	0.0	0.12515617737428447	0.0
72-73	0.0	3.928429023019344E-4	0.0	0.12943280806070778	0.0
74-75	0.0	3.928429023019344E-4	0.0	0.1332451698625925	0.0
76-77	0.0	4.1069939786111324E-4	0.0	0.13699503393002005	0.0
78-79	0.0	4.1069939786111324E-4	0.0	0.1395574410427622	0.0
80-81	0.0	4.1069939786111324E-4	0.0	0.14026277261734976	0.0
82-83	0.0	4.1069939786111324E-4	0.0	0.14078953923634555	0.0
84-85	0.0	4.1069939786111324E-4	0.0	0.14103953017417403	0.0
86-87	0.0	4.1069939786111324E-4	0.0	0.1411645256430883	0.0
88	0.0	4.2855589342029207E-4	0.0	0.14117345389086788	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6945	0.0	51.009037	1
GTATCAA	13045	0.0	38.997383	1
ATCAACG	15910	0.0	31.129858	3
CAACGCA	16160	0.0	30.518404	5
TCAACGC	16200	0.0	30.49487	4
TATCAAC	16560	0.0	30.314318	2
AACGCAG	17620	0.0	28.132563	6
ACGCAGA	18625	0.0	26.479324	7
CGCAGAG	20005	0.0	24.631727	8
CTCGATA	715	0.0	21.351336	94
GCAGAGT	23180	0.0	21.27599	9
GTGGTAT	3705	0.0	20.618647	1
TACATGG	18350	0.0	20.609415	2
GAGTACT	12240	0.0	20.52334	12-13
GTACATG	19140	0.0	20.35087	1
ACATGGG	18255	0.0	20.164295	3
CAGAGTA	22445	0.0	18.812376	10-11
AGAGTAC	20895	0.0	18.761597	10-11
CATGGGG	13430	0.0	18.626684	4
TGGTATC	3800	0.0	18.556782	2
>>END_MODULE