FastQCFastQC Report
Fri 27 May 2016
SRR2139388_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139388_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8402465
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108950.048.8005331
GTATCAA193950.037.3898621
CTTATAC72900.035.5856861
TCAACGC225300.031.4436844
ATCAACG226900.031.3162823
CAACGCA231200.030.67835
TATCAAC237450.030.17962
AACGCAG241750.029.4457346
TATACAC105700.029.3197463
ACGCAGA274750.025.8934527
CGCAGAG280200.025.4050888
TTATACA115500.025.0179672
GTGGTAT53700.024.9517061
TGGTATC51550.024.3315162
ACACATC123400.022.7554446
ACATCTC124450.022.2194988
TACACAT140100.021.8456275
GCAGAGT334800.021.2108259
GTACATG257350.020.5767021
TACATGG251500.020.4085162
Produced by FastQC (version 0.11.5)