##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139388_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8402465
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.365003008045854	30.0	18.0	33.0	18.0	34.0
2	31.51066811941496	32.0	32.0	33.0	27.0	34.0
3	32.35200301340142	33.0	33.0	33.0	30.0	34.0
4	32.743687001374	33.0	33.0	34.0	31.0	34.0
5	33.08799727222904	33.0	33.0	34.0	32.0	34.0
6	36.36628632193053	38.0	37.0	38.0	34.0	38.0
7	37.03872446954554	38.0	38.0	38.0	35.0	38.0
8	36.53685579172303	38.0	37.0	38.0	34.0	38.0
9	37.14559406079049	38.0	38.0	38.0	36.0	38.0
10-11	37.29919017812035	38.0	38.0	38.0	36.5	38.0
12-13	37.378486848799724	38.0	38.0	38.0	37.0	38.0
14-15	37.3310059607508	38.0	38.0	38.0	36.5	38.0
16-17	37.40398942453197	38.0	38.0	38.0	37.0	38.0
18-19	37.423303637682515	38.0	38.0	38.0	37.0	38.0
20-21	37.45502658899975	38.0	38.0	38.0	37.0	38.0
22-23	37.46604653198189	38.0	38.0	38.0	37.0	38.0
24-25	37.484969215073306	38.0	38.0	38.0	37.0	38.0
26-27	37.45455496297666	38.0	38.0	38.0	37.0	38.0
28-29	37.43871510573993	38.0	38.0	38.0	37.0	38.0
30-31	37.448393639998216	38.0	38.0	38.0	37.0	38.0
32-33	37.4271981312631	38.0	38.0	38.0	37.0	38.0
34-35	37.40596906589208	38.0	38.0	38.0	37.0	38.0
36-37	37.38038664153374	38.0	38.0	38.0	37.0	38.0
38-39	37.36238560499962	38.0	38.0	38.0	37.0	38.0
40-41	37.3714340166711	38.0	38.0	38.0	37.0	38.0
42-43	37.33893031337517	38.0	38.0	38.0	37.0	38.0
44-45	37.30794177883817	38.0	38.0	38.0	37.0	38.0
46-47	37.28110632121155	38.0	38.0	38.0	37.0	38.0
48-49	37.22531741391499	38.0	38.0	38.0	37.0	38.0
50-51	37.144596871222305	38.0	38.0	38.0	36.5	38.0
52-53	37.128605494238556	38.0	38.0	38.0	36.0	38.0
54-55	37.06227934914753	38.0	38.0	38.0	36.0	38.0
56-57	37.029018745248166	38.0	38.0	38.0	36.0	38.0
58-59	37.026145579349404	38.0	38.0	38.0	36.0	38.0
60-61	37.03054662744158	38.0	38.0	38.0	36.0	38.0
62-63	37.03401482517121	38.0	38.0	38.0	36.0	38.0
64-65	37.0376422844204	38.0	38.0	38.0	36.0	38.0
66-67	37.02974126636977	38.0	38.0	38.0	36.0	38.0
68-69	37.01626219379048	38.0	38.0	38.0	36.0	38.0
70-71	37.004724197848844	38.0	38.0	38.0	36.0	38.0
72-73	37.00689211988107	38.0	38.0	38.0	36.0	38.0
74-75	36.99145429525181	38.0	38.0	38.0	36.0	38.0
76-77	36.98306400249322	38.0	38.0	38.0	36.0	38.0
78-79	36.95443339601182	38.0	38.0	38.0	36.0	38.0
80-81	36.94612310641724	38.0	38.0	38.0	35.5	38.0
82-83	36.887529123096286	38.0	38.0	38.0	35.0	38.0
84-85	36.86763014792792	38.0	38.0	38.0	35.0	38.0
86-87	36.85669758511601	38.0	38.0	38.0	35.0	38.0
88-89	36.831347631681766	38.0	38.0	38.0	35.0	38.0
90-91	36.825891681437994	38.0	38.0	38.0	35.0	38.0
92-93	36.823483743432895	38.0	38.0	38.0	35.0	38.0
94-95	36.823020590832186	38.0	38.0	38.0	35.0	38.0
96-97	36.8367930259588	38.0	38.0	38.0	35.0	38.0
98-99	36.81950698855185	38.0	38.0	38.0	35.0	38.0
100	35.10806782910311	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	3.0
20	15.0
21	112.0
22	440.0
23	1244.0
24	2936.0
25	6339.0
26	11578.0
27	19618.0
28	31199.0
29	46207.0
30	62850.0
31	81379.0
32	106585.0
33	147229.0
34	226604.0
35	430778.0
36	1371255.0
37	5856092.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.52755649681373	18.108555049024304	13.514260398585416	22.84962805557655
2	14.692419149472519	20.20440167360431	43.09881020987181	22.00436896705136
3	18.465807355341557	26.19564615859751	30.269831531580316	25.06871495448062
4	12.631995491799133	18.525670740669554	38.50990155864975	30.33243220888156
5	13.891649652810218	37.44122706848526	35.3666453832298	13.300477895474721
6	30.186736868288055	37.64279886914137	18.762339384930492	13.408124877640073
7	27.07914879740648	32.67159101525564	22.598844505749206	17.650415681588676
8	24.517198226948878	36.92128440880146	20.98701988047555	17.574497483774106
9	25.756929662902493	17.484833319746052	21.01561863096127	35.742618386390184
10-11	24.50287504916712	26.87166206583425	28.613769887765077	20.01169299723355
12-13	25.428109489298677	24.21143081226759	28.744451776948786	21.616007921484943
14-15	22.50701431068145	25.612472054331676	26.393272688431313	25.487240946555563
16-17	22.12006238645445	28.869653131551274	27.043992447454407	21.966292034539865
18-19	22.11290972351566	27.818711532865652	28.8419410256395	21.226437717979188
20-21	22.84748436692565	26.961331661108318	28.722398587637926	21.468785384328104
22-23	22.581989289863742	27.024902245072504	28.585321300517226	21.80778716454653
24-25	22.41536996809082	27.151507137223053	28.71589497167047	21.71722792301566
26-27	22.314078854479323	27.29355574386106	28.713653935054918	21.678711466604703
28-29	22.068626676969348	27.23327490145351	28.895064703155455	21.803033718421688
30-31	22.475763947905264	26.98998843267481	28.609288816581767	21.924958802838155
32-33	22.427512396171245	27.16131558807341	28.747750899341778	21.66342111641357
34-35	22.48686317869338	27.406746744704208	28.61797618004005	21.48841389656236
36-37	22.30587899006962	27.2591545921062	28.57580287807759	21.85916353974659
38-39	22.13396347219149	27.276316754656083	28.811688560631193	21.77803121252123
40-41	22.437152555236022	27.124427746520624	28.72863540954124	21.70978428870211
42-43	22.50348197180267	27.176249943100046	28.565287085863787	21.754980999233492
44-45	22.33830262490234	27.411214185421134	28.461452064599253	21.789031125077273
46-47	22.60444216533749	27.208278205158592	28.05587970915407	22.131399920349846
48-49	22.49654159387554	27.32653553951417	28.085636210649234	22.091286655961056
50-51	22.317109222001346	27.51279083131625	28.171698402622138	21.99840154406027
52-53	22.55557301712027	27.451414783595652	28.195302839091678	21.797709360192393
54-55	22.480267798953797	27.445216400255575	27.96573974408418	22.108776056706454
56-57	22.676710201808397	27.689352328187873	27.701753903981235	21.932183566022495
58-59	22.39804992524796	27.884348483596327	28.05211062274859	21.665490968407127
60-61	22.642231640565807	28.030492309741273	27.688693565749578	21.638582483943345
62-63	22.400584601163985	28.298667237524946	27.651707735096654	21.64904042621442
64-65	22.40929853124558	28.19963984055336	27.67825613962055	21.712805488580514
66-67	22.62160465625617	28.174350770078515	27.527132077035294	21.676912496630017
68-69	22.475514522502003	28.068412712687667	27.724474370213937	21.731598394596393
70-71	22.618899654021103	27.681900150633638	27.761080426481822	21.93811976886344
72-73	22.679293764434053	27.547409331973384	27.807302228980557	21.96599467461201
74-75	22.467757386974924	27.544535001805272	27.891984422344024	22.09572318887578
76-77	22.48764039501449	27.57019378948527	27.813087450357283	22.129078365142963
78-79	22.511939834502552	27.60973936997943	27.722409537109943	22.15591125840808
80-81	22.478310887047474	27.5616957196939	27.761082378943996	22.198911014314632
82-83	22.54110262953505	27.503306219077622	27.874455032063977	22.08113611932335
84-85	22.642158451430426	27.464195221119002	27.787277923455406	22.10636840399517
86-87	22.397142410295203	27.526841961340793	27.856740046184242	22.219275582179762
88-89	22.51845275091305	27.533374298126073	27.776961804249943	22.17121114671093
90-91	22.562164432046554	27.557598752515872	27.799000752150615	22.081236063286962
92-93	22.594057303634465	27.575198747373953	27.71088915523049	22.119854793761096
94-95	22.43312683920358	27.70705341972989	27.715709637087116	22.144110103979415
96-97	22.54514290383031	27.706890484639434	27.83299392921141	21.914972682318844
98-99	23.60734552870482	29.717419328436083	27.99183905929215	18.68339608356694
100	22.58197815094904	30.561766488606217	21.914661990407	24.941593370037737
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1048.0
1	999.0
2	1291.0
3	1827.5
4	2462.0
5	3645.5
6	4641.0
7	5725.0
8	7173.5
9	8143.0
10	8491.0
11	8694.5
12	9552.0
13	11983.0
14	16860.5
15	22585.0
16	27583.0
17	31200.0
18	31959.0
19	30015.5
20	27198.0
21	25077.0
22	25508.0
23	29145.5
24	35902.5
25	46804.5
26	62107.5
27	78943.5
28	94347.0
29	111095.0
30	127958.5
31	144766.0
32	164348.0
33	185095.5
34	206099.5
35	225576.0
36	248097.5
37	268638.0
38	281572.0
39	293628.5
40	309560.0
41	324530.0
42	334120.5
43	342799.0
44	356224.0
45	377049.0
46	382272.5
47	376583.0
48	373011.5
49	361239.5
50	348554.5
51	336834.5
52	321616.5
53	302989.5
54	283671.5
55	262585.0
56	239410.0
57	214649.0
58	190268.0
59	166097.0
60	139522.0
61	112745.5
62	88658.5
63	68866.0
64	52260.0
65	38669.0
66	28376.5
67	21353.0
68	16281.5
69	11899.5
70	8611.5
71	6334.5
72	4517.0
73	3019.5
74	2060.5
75	1421.0
76	911.5
77	530.0
78	330.0
79	214.0
80	137.0
81	89.5
82	60.0
83	43.5
84	32.5
85	22.5
86	13.5
87	11.0
88	6.5
89	2.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.005700708066025863
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	7.735899255741034E-5
22-23	0.0
24-25	2.737547728549528E-4
26-27	0.002547356578914399
28-29	0.006411972635343972
30-31	0.006626146667492155
32-33	0.0017491040915723448
34-35	6.163339998915731E-4
36-37	7.806660017794381E-5
38-39	5.433589909461299E-4
40-41	7.895344176272669E-5
42-43	1.0387095268360382E-4
44-45	1.2924018586954274E-4
46-47	5.584691442384941E-5
48-49	7.82049450050314E-4
50-51	4.350121481870774E-4
52-53	0.0
54-55	0.007674199533024958
56-57	0.061692485517540786
58-59	0.07360870834747053
60-61	0.04585886580280792
62-63	0.008899047147904939
64-65	6.662705466176842E-5
66-67	0.0
68-69	5.574003770881527E-4
70-71	0.003038428420332063
72-73	0.012153513616941275
74-75	0.010649462896653908
76-77	0.015874085788600792
78-79	0.006613340029131584
80-81	0.00722876044306741
82-83	0.04110834397268236
84-85	0.025964795389901242
86-87	0.008759826454304082
88-89	0.0023214012308384858
90-91	7.5936452124420975E-6
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	45.0
20-21	198.0
22-23	435.0
24-25	619.0
26-27	1823.0
28-29	4878.0
30-31	14706.0
32-33	18294.0
34-35	25676.0
36-37	41392.0
38-39	49737.0
40-41	48400.0
42-43	55889.0
44-45	65641.0
46-47	67070.0
48-49	62265.0
50-51	57339.0
52-53	55018.0
54-55	59730.0
56-57	62434.0
58-59	62302.0
60-61	64441.0
62-63	63348.0
64-65	69416.0
66-67	76373.0
68-69	77367.0
70-71	89592.0
72-73	65153.0
74-75	64428.0
76-77	67736.0
78-79	69393.0
80-81	69750.0
82-83	65346.0
84-85	65616.0
86-87	67716.0
88-89	70370.0
90-91	71445.0
92-93	71587.0
94-95	82353.0
96-97	259714.0
98-99	575679.0
100-101	5541751.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.91530240426856
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.85003631184492	76.29643004454822
2	7.053790876787816	11.97949570797816
3	1.688560247562574	4.301538121488076
4	0.6256600790648449	2.125124592642795
5	0.2931805934819246	1.2447759377290277
6	0.16434629715323132	0.8373309313073051
7	0.09288789834701772	0.5521322784483776
8	0.05831026928362694	0.39611473195948116
9	0.0422341834664381	0.3227695614764955
>10	0.12777519622756833	1.6530250097858432
>50	0.0026164546757409847	0.14593734647560277
>100	4.8617678049708E-4	0.0750170259945629
>500	9.233258972075036E-5	0.04572934021896656
>1k	2.308273412160742E-5	0.02457936994710043
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.57038083467173E-5	0.0	1.1901269448905767E-5	0.0
3	0.0	3.57038083467173E-5	0.0	2.3802538897811534E-5	0.0
4	0.0	7.14076166934346E-5	0.0	2.3802538897811534E-5	0.0
5	0.0	7.14076166934346E-5	0.0	2.3802538897811534E-5	0.0
6	0.0	7.14076166934346E-5	0.0	2.3802538897811534E-5	0.0
7	0.0	7.14076166934346E-5	0.0	2.3802538897811534E-5	0.0
8	0.0	9.521015559124614E-5	0.0	2.3802538897811534E-5	2.3802538897811534E-5
9	0.0	1.9042031118249227E-4	0.0	7.14076166934346E-5	2.3802538897811534E-5
10-11	0.0	1.9042031118249227E-4	0.0	1.1306205976460479E-4	2.3802538897811534E-5
12-13	0.0	1.9637094590694516E-4	0.0	1.1901269448905767E-4	2.3802538897811534E-5
14-15	0.0	2.7372919732483264E-4	0.0	1.1901269448905767E-4	2.3802538897811534E-5
16-17	0.0	2.856304667737384E-4	0.0	1.428152333868692E-4	2.3802538897811534E-5
18-19	0.0	3.0943300567154994E-4	0.0	1.785190417335865E-4	2.3802538897811534E-5
20-21	0.0	3.332355445693615E-4	0.0	2.0232158063139804E-4	2.3802538897811534E-5
22-23	0.0	3.8084062236498455E-4	0.0	2.142228500803038E-4	2.3802538897811534E-5
24-25	0.0	4.10593795987249E-4	0.0	2.2612411952920957E-4	2.3802538897811534E-5
26-27	0.0	4.343963348850605E-4	0.0	2.856304667737384E-4	2.3802538897811534E-5
28-29	0.0	4.403469696095134E-4	0.0	4.6414950850732497E-4	2.3802538897811534E-5
30-31	0.0	4.8795204740513645E-4	0.0	0.0012734358310329172	2.3802538897811534E-5
32-33	0.0	4.8795204740513645E-4	0.0	0.0028920084760841016	2.3802538897811534E-5
34-35	0.0	5.177052210274008E-4	0.0	0.0047069520670422315	2.3802538897811534E-5
36-37	0.0	5.593596640985711E-4	0.0	0.007723923872339843	2.3802538897811534E-5
38-39	0.0	5.593596640985711E-4	0.0	0.013031890046551815	2.3802538897811534E-5
40-41	0.0	6.188660113430999E-4	0.0	0.01797686750257216	2.3802538897811534E-5
42-43	0.0	6.367179155164586E-4	0.0	0.023278883042059682	2.3802538897811534E-5
44-45	0.0	6.843229933120817E-4	0.0	0.02860470112044501	2.9753173622264418E-5
46-47	0.0	7.14076166934346E-4	0.0	0.0345672371143468	3.57038083467173E-5
48-49	0.0	7.259774363832518E-4	0.0	0.04079160103612452	4.760507779562307E-5
50-51	0.0	8.152369572500451E-4	0.0	0.04660537116191499	4.760507779562307E-5
52-53	0.0	8.21187591974498E-4	0.0	0.05227632605431859	5.355571252007595E-5
54-55	0.0	8.271382266989509E-4	0.0	0.057935379677273274	5.9506347244528835E-5
56-57	0.0	8.449901308723095E-4	0.0	0.06410023725180647	5.9506347244528835E-5
58-59	0.0	8.509407655967623E-4	0.0	0.07030674926941083	5.9506347244528835E-5
60-61	0.0	8.68792669770121E-4	0.0	0.07612647002992574	6.545698196898172E-5
62-63	0.0	8.925952086679326E-4	0.0	0.08191643761681841	8.330888614234037E-5
64-65	0.0	9.878053642591786E-4	0.0	0.08834907375395196	8.330888614234037E-5
66-67	0.0	9.997066337080844E-4	0.0	0.0940021767421822	8.330888614234037E-5
68-69	0.0	0.0010592129809526133	0.0	0.09948866195812776	8.330888614234037E-5
70-71	0.0	0.0011127686934726893	0.0	0.10432652798910796	8.330888614234037E-5
72-73	0.0	0.0011187193281971422	0.0	0.10839676214063373	9.521015559124614E-5
74-75	0.0	0.0011425218670949536	0.0	0.11141373394593135	9.521015559124614E-5
76-77	0.0	0.0011722750407172182	0.0	0.1146508792360337	9.521015559124614E-5
78-79	0.0	0.0011782256754416709	0.0	0.11688236725770354	9.521015559124614E-5
80-81	0.0	0.0011782256754416709	0.0	0.11764404850243351	9.521015559124614E-5
82-83	0.0	0.0011782256754416709	0.0	0.11816175372346091	9.521015559124614E-5
84-85	0.0	0.0011782256754416709	0.0	0.11847713736385691	9.521015559124614E-5
86-87	0.0	0.0011901269448905767	0.0	0.11864970577086605	9.521015559124614E-5
88	0.0	0.0012377320226861998	0.0	0.1186675576750394	9.521015559124614E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10895	0.0	48.800533	1
GTATCAA	19395	0.0	37.389862	1
CTTATAC	7290	0.0	35.585686	1
TCAACGC	22530	0.0	31.443684	4
ATCAACG	22690	0.0	31.316282	3
CAACGCA	23120	0.0	30.6783	5
TATCAAC	23745	0.0	30.1796	2
AACGCAG	24175	0.0	29.445734	6
TATACAC	10570	0.0	29.319746	3
ACGCAGA	27475	0.0	25.893452	7
CGCAGAG	28020	0.0	25.405088	8
TTATACA	11550	0.0	25.017967	2
GTGGTAT	5370	0.0	24.951706	1
TGGTATC	5155	0.0	24.331516	2
ACACATC	12340	0.0	22.755444	6
ACATCTC	12445	0.0	22.219498	8
TACACAT	14010	0.0	21.845627	5
GCAGAGT	33480	0.0	21.210825	9
GTACATG	25735	0.0	20.576702	1
TACATGG	25150	0.0	20.408516	2
>>END_MODULE