FastQCFastQC Report
Fri 27 May 2016
SRR2139387_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139387_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5901661
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92150.053.0506061
GTATCAA174600.040.029531
ATCAACG215300.031.8735943
TATCAAC218350.031.7771512
CAACGCA218950.031.20745
TCAACGC220300.030.9970154
AACGCAG230650.029.7889466
ACGCAGA250050.027.3765817
CGCAGAG262250.026.0869248
GCAGAGT296100.023.1759039
GTGGTAT45450.022.4586521
TGGTATC44250.021.161282
TACATGG214550.020.7801512
GAGTACT175350.020.69820212-13
GTACATG220050.020.6441291
ACATGGG211550.020.4560533
GTACTAG20050.020.406081
CTCGATA10150.020.17081894
CAGAGTA292800.019.88624810-11
AGAGTAC280900.018.82926410-11
Produced by FastQC (version 0.11.5)