##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139387_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5901661
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.31381267070406	30.0	18.0	33.0	18.0	34.0
2	31.50002194297504	32.0	32.0	33.0	27.0	34.0
3	32.34458095780154	33.0	33.0	33.0	30.0	34.0
4	32.74205465207168	33.0	33.0	34.0	31.0	34.0
5	33.07636528089296	33.0	33.0	34.0	32.0	34.0
6	36.33810481489872	38.0	36.0	38.0	33.0	38.0
7	37.02885458856414	38.0	38.0	38.0	35.0	38.0
8	36.5622757728714	38.0	37.0	38.0	34.0	38.0
9	37.16031283396318	38.0	38.0	38.0	36.0	38.0
10-11	37.304526895055474	38.0	38.0	38.0	36.5	38.0
12-13	37.38744024775398	38.0	38.0	38.0	37.0	38.0
14-15	37.34523162208063	38.0	38.0	38.0	36.5	38.0
16-17	37.41255639725833	38.0	38.0	38.0	37.0	38.0
18-19	37.44024284010891	38.0	38.0	38.0	37.0	38.0
20-21	37.46870720638212	38.0	38.0	38.0	37.0	38.0
22-23	37.480019441784314	38.0	38.0	38.0	37.0	38.0
24-25	37.4990173518424	38.0	38.0	38.0	37.0	38.0
26-27	37.4713686236944	38.0	38.0	38.0	37.0	38.0
28-29	37.456959994595714	38.0	38.0	38.0	37.0	38.0
30-31	37.46278679924281	38.0	38.0	38.0	37.0	38.0
32-33	37.44236043880666	38.0	38.0	38.0	37.0	38.0
34-35	37.42126735699793	38.0	38.0	38.0	37.0	38.0
36-37	37.39469112258988	38.0	38.0	38.0	37.0	38.0
38-39	37.39048322918312	38.0	38.0	38.0	37.0	38.0
40-41	37.371876359555216	38.0	38.0	38.0	37.0	38.0
42-43	37.33658409760731	38.0	38.0	38.0	37.0	38.0
44-45	37.299616085087536	38.0	38.0	38.0	37.0	38.0
46-47	37.26251836054201	38.0	38.0	38.0	37.0	38.0
48-49	37.20308047187257	38.0	38.0	38.0	37.0	38.0
50-51	37.12418162863376	38.0	38.0	38.0	36.5	38.0
52-53	37.11403487146819	38.0	38.0	38.0	36.0	38.0
54-55	37.0551742300079	38.0	38.0	38.0	36.0	38.0
56-57	37.02361928221545	38.0	38.0	38.0	36.0	38.0
58-59	37.029325290527304	38.0	38.0	38.0	36.0	38.0
60-61	37.041897020580436	38.0	38.0	38.0	36.0	38.0
62-63	37.05190087721763	38.0	38.0	38.0	36.0	38.0
64-65	37.05757822211348	38.0	38.0	38.0	36.0	38.0
66-67	37.055939213936334	38.0	38.0	38.0	36.0	38.0
68-69	37.05145461478588	38.0	38.0	38.0	36.0	38.0
70-71	37.04522089848162	38.0	38.0	38.0	36.0	38.0
72-73	37.05065762695186	38.0	38.0	38.0	36.0	38.0
74-75	37.0392504092757	38.0	38.0	38.0	36.0	38.0
76-77	37.03376697325653	38.0	38.0	38.0	36.0	38.0
78-79	37.00813380512767	38.0	38.0	38.0	36.0	38.0
80-81	37.00073008763855	38.0	38.0	38.0	36.0	38.0
82-83	36.94578213719117	38.0	38.0	38.0	35.0	38.0
84-85	36.92520284309079	38.0	38.0	38.0	35.0	38.0
86-87	36.92141045656365	38.0	38.0	38.0	35.0	38.0
88-89	36.896566788197234	38.0	38.0	38.0	35.0	38.0
90-91	36.890317588736494	38.0	38.0	38.0	35.0	38.0
92-93	36.88808302514703	38.0	38.0	38.0	35.0	38.0
94-95	36.89171246226347	38.0	38.0	38.0	35.0	38.0
96-97	36.90735570006867	38.0	38.0	38.0	35.0	38.0
98-99	36.896537429504704	38.0	38.0	38.0	35.0	38.0
100	35.31211424134175	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	10.0
21	59.0
22	264.0
23	744.0
24	1876.0
25	3951.0
26	7565.0
27	13480.0
28	22516.0
29	34019.0
30	44732.0
31	56011.0
32	72466.0
33	97663.0
34	150377.0
35	286607.0
36	945514.0
37	4163804.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.220987616875995	18.05644207622227	13.297358150527453	22.425212156374283
2	15.222919477797692	20.12100198071946	42.306686677768	22.349391863714843
3	18.327958857684305	26.716902241589274	30.08896986797446	24.866169032751966
4	12.700577007049372	18.195606287789147	38.76452747794223	30.33928922721925
5	13.806536837680103	37.44649175884552	35.097492045036134	13.649479358438244
6	30.1577301712179	37.642267151569705	18.600712579051898	13.599290098160502
7	27.027018325857753	32.710231916065666	22.97356625533049	17.28918350274609
8	25.141328856401614	35.725518629416364	20.846656559907455	18.286495954274567
9	26.346023602507834	16.820027446510398	21.33265533211752	35.50129361886425
10-11	24.839718852031655	26.496404995136114	28.34288516402416	20.320990988808067
12-13	25.764814346334024	24.06814793326828	28.48389631325825	21.68314140713945
14-15	22.961891575947856	25.180224685897752	26.58847229618916	25.269411441965232
16-17	22.08257980253356	28.20533066877274	27.222666635714926	22.489422892978773
18-19	22.0153868546499	27.228080365849543	29.39130187247285	21.365230907027698
20-21	23.072713663184143	26.43403513364791	29.127275915926298	21.365975287241653
22-23	23.001368907706205	26.18864390361609	28.91592795327837	21.89405923539934
24-25	22.506692113304645	26.420705659538353	29.04535622581756	22.02724600133944
26-27	22.417012210778942	26.635429987381603	29.101915810115564	21.84564199172389
28-29	22.24943120564057	26.71327575699925	29.112004248535246	21.925288788824943
30-31	22.632000701431373	26.542107144321285	28.88299006002823	21.942902094219107
32-33	22.446662848910425	26.71308200792201	28.803779918942436	22.03647522422513
34-35	22.538725859628176	26.762368498775867	28.844036481229686	21.85486916036627
36-37	22.581848954259577	26.569604401075942	28.814193565852598	22.03435307881189
38-39	22.47155184886469	26.698072715038396	28.944022495366777	21.886352940730134
40-41	22.63209692640146	26.522404234146745	28.931364642456835	21.914134196994958
42-43	22.686435886159455	26.562269488850852	28.55862838614589	22.19266623884381
44-45	22.667846992169647	26.96736833174433	28.285911516728575	22.07887315935745
46-47	22.70613719210166	26.83417059429145	28.001058268831326	22.458633944775563
48-49	22.807945578015502	26.683193294955437	28.072120357878493	22.436740769150564
50-51	22.61132196717947	26.958358205778097	27.921165160250105	22.509154666792323
52-53	22.727932467308463	27.045499793111865	27.665109118583143	22.56145862099653
54-55	22.901025937444302	26.90154528229984	27.61265535694224	22.584773423313624
56-57	22.896835173067885	26.95437605353106	27.663562040619333	22.48522673278172
58-59	22.809153715499058	26.937296220288893	27.85698789299269	22.396562171219355
60-61	22.921822908308386	27.10009652551021	27.592419485486175	22.385661080695233
62-63	22.898341588206456	26.992032236187573	27.64223681598465	22.467389359621322
64-65	22.75753127492451	26.89664843912446	27.6846314508896	22.661188835061434
66-67	22.963977504049772	26.95540903628933	27.498657258644755	22.581956201016144
68-69	22.88266132628586	26.88398738050936	27.791299100557893	22.442052192646894
70-71	22.991660924976742	26.53684343867425	27.766922542918593	22.70457309343042
72-73	23.02518201971771	26.71278867851364	27.713885747638905	22.548143554129744
74-75	22.708930071904454	26.92854342260786	27.726117062416893	22.636409443070796
76-77	22.79108575942673	26.915506504838966	27.54183069239516	22.75157704333915
78-79	22.88255672439271	26.88323020187195	27.4718545322068	22.762358541528535
80-81	22.768536483022725	26.966443394130007	27.53408024264708	22.73093988020019
82-83	23.085743113901923	26.803852198651878	27.733411548683172	22.376993138763027
84-85	23.140593612839748	26.816070788217083	27.31031391579218	22.733021683150987
86-87	22.696571573267953	27.05659464724068	27.603409752383385	22.64342402710798
88-89	22.986287207237943	26.852730373557755	27.48519962870889	22.675782790495415
90-91	23.03207597931657	26.90188543739473	27.42729315508292	22.638745428205777
92-93	23.09613336341162	26.965594044498854	27.39461795980057	22.543654632288952
94-95	22.809475807841082	27.119906584437203	27.364368937257836	22.706248670463875
96-97	22.93784225257105	27.14716102379776	27.433216594799255	22.48178012883194
98-99	24.064554518304085	29.05932188920991	27.539475522994167	19.336648069491844
100	31.163874045258023	40.09791929607122	28.73820665867075	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	946.0
1	994.0
2	1522.0
3	2147.0
4	2939.5
5	4506.0
6	5737.5
7	6773.0
8	8114.0
9	8796.5
10	8548.5
11	8297.0
12	9172.5
13	11776.5
14	16899.0
15	23145.5
16	28380.0
17	31980.0
18	32327.0
19	29124.5
20	24841.5
21	21151.0
22	19533.5
23	21011.0
24	25189.5
25	31617.5
26	41548.0
27	52900.5
28	61955.0
29	72509.0
30	83757.0
31	94330.0
32	108992.5
33	125759.0
34	139889.0
35	155685.0
36	175343.5
37	190124.0
38	196728.5
39	199695.5
40	204936.0
41	210635.0
42	215249.0
43	222980.0
44	233960.0
45	242734.5
46	245056.0
47	249145.5
48	254965.0
49	257436.0
50	257622.0
51	255687.5
52	249386.5
53	238991.0
54	226404.0
55	210699.0
56	194013.0
57	178527.5
58	162285.0
59	139313.0
60	112059.5
61	87162.0
62	66474.5
63	49947.5
64	36881.0
65	27119.5
66	19596.0
67	14442.0
68	11222.5
69	8360.0
70	6107.0
71	4539.0
72	3289.0
73	2354.0
74	1626.0
75	1006.5
76	608.0
77	371.0
78	236.0
79	155.5
80	106.0
81	75.0
82	47.5
83	26.0
84	13.5
85	7.0
86	4.5
87	3.5
88	2.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004574983212353268
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	6.777809755860751E-5
22-23	0.0
24-25	4.2365693549280877E-4
26-27	0.0028134253266920675
28-29	0.0064174943779103915
30-31	0.006883127883423747
32-33	0.0022712071414918667
34-35	7.7632829985382E-4
36-37	1.1993698853299587E-4
38-39	5.604496220025969E-4
40-41	8.68478033932479E-5
42-43	1.2250162314650668E-4
44-45	1.1477172081302169E-4
46-47	7.135995118979339E-5
48-49	8.380694772212716E-4
50-51	4.457461850266943E-4
52-53	0.0
54-55	0.007189984656739306
56-57	0.06161775899096693
58-59	0.07332599884749183
60-61	0.04505667449946442
62-63	0.009310007393241166
64-65	1.1637889398930168E-4
66-67	0.0
68-69	5.246379898881471E-4
70-71	0.0031317327877514625
72-73	0.012276928264006948
74-75	0.01058523582342151
76-77	0.015480888125848984
78-79	0.006694636530845617
80-81	0.007755414922622831
82-83	0.04135390703569017
84-85	0.02574499720771619
86-87	0.00887718092219479
88-89	0.0022234493597793276
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	25.0
20-21	133.0
22-23	414.0
24-25	536.0
26-27	1756.0
28-29	4477.0
30-31	12811.0
32-33	15821.0
34-35	21456.0
36-37	34461.0
38-39	42497.0
40-41	41858.0
42-43	48104.0
44-45	57053.0
46-47	58026.0
48-49	53606.0
50-51	47569.0
52-53	45456.0
54-55	49679.0
56-57	51065.0
58-59	50063.0
60-61	48750.0
62-63	47831.0
64-65	50767.0
66-67	53034.0
68-69	53456.0
70-71	54000.0
72-73	50733.0
74-75	51402.0
76-77	53647.0
78-79	55146.0
80-81	54978.0
82-83	51980.0
84-85	52509.0
86-87	52929.0
88-89	55275.0
90-91	56398.0
92-93	54494.0
94-95	62699.0
96-97	181074.0
98-99	1349776.0
100-101	2773917.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.26507851606769
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.07800775200424	71.57666215505444
2	7.606426300708937	12.362736611075881
3	2.1992399930796704	5.361642321398867
4	0.9324356631981704	3.0309782952392386
5	0.428114912922393	1.7395396006268875
6	0.2453229106489505	1.1961711357406333
7	0.15000496728757945	0.8533115811097716
8	0.09940971885658476	0.646283088651246
9	0.06021747545512683	0.4404220083810235
>10	0.19685836769704956	2.320329501394462
>50	0.002702097269898987	0.1450187828259701
>100	0.0010703851615087112	0.19141894771118165
>500	1.1367342700449433E-4	0.0561958863071011
>1k	7.578228282322074E-5	0.07929008448335696
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.6944382267975065E-5	0.0
3	0.0	0.0	0.0	1.6944382267975065E-5	0.0
4	0.0	5.08331468039252E-5	0.0	3.388876453595013E-5	0.0
5	0.0	5.08331468039252E-5	0.0	3.388876453595013E-5	0.0
6	0.0	5.08331468039252E-5	0.0	3.388876453595013E-5	0.0
7	0.0	5.08331468039252E-5	0.0	3.388876453595013E-5	0.0
8	0.0	6.777752907190026E-5	0.0	3.388876453595013E-5	0.0
9	0.0	1.3555505814380052E-4	0.0	5.08331468039252E-5	0.0
10-11	0.0	1.3555505814380052E-4	0.0	1.0166629360785039E-4	0.0
12-13	0.0	1.3555505814380052E-4	0.0	1.27082867009813E-4	0.0
14-15	0.0	1.3555505814380052E-4	0.0	1.3555505814380052E-4	0.0
16-17	0.0	1.3555505814380052E-4	0.0	1.779160138137382E-4	0.0
18-19	0.0	1.4402724927778805E-4	0.0	1.8638820494772573E-4	0.0
20-21	0.0	1.524994404117756E-4	0.0	2.2027696948367586E-4	0.0
22-23	0.0	2.1180477834968834E-4	0.0	2.7111011628760105E-4	0.0
24-25	0.0	2.3722135175165094E-4	0.0	3.049988808235512E-4	0.0
26-27	0.0	2.3722135175165094E-4	0.0	3.558320276274764E-4	0.0
28-29	0.0	2.3722135175165094E-4	0.0	7.031918641209652E-4	0.0
30-31	0.0	3.049988808235512E-4	0.0	0.00192318738741517	0.0
32-33	0.0	3.049988808235512E-4	0.0	0.004515677874415355	0.0
34-35	0.0	3.2194326309152626E-4	0.0	0.008218025399967906	0.0
36-37	0.0	3.388876453595013E-4	0.0	0.013513144858710115	0.0
38-39	0.0	3.388876453595013E-4	0.0	0.02205311352176955	0.0
40-41	0.0	3.7277640989545147E-4	0.0	0.03117766337307412	0.0
42-43	0.0	3.81248601029439E-4	0.0	0.03986165928541134	0.0
44-45	0.0	4.1513736556538915E-4	0.0	0.049138708577127696	0.0
46-47	0.0	4.4055393896735173E-4	0.0	0.05929686574677874	0.0
48-49	0.0	4.4055393896735173E-4	0.0	0.06997182657560304	0.0
50-51	0.0	4.829148946372894E-4	0.0	0.08068067616896328	0.0
52-53	0.0	4.913870857712769E-4	0.0	0.09067786170706857	0.0
54-55	0.0	4.998592769052644E-4	0.0	0.0994889404864156	0.0
56-57	0.0	5.08331468039252E-4	0.0	0.1062921099670076	0.0
58-59	0.0	5.08331468039252E-4	0.0	0.11239208758347861	0.0
60-61	0.0	5.25275850307227E-4	0.0	0.11855984272902154	0.0
62-63	0.0	5.25275850307227E-4	0.0	0.12492245827064619	0.0
64-65	0.0	5.422202325752021E-4	0.0	0.1310224358871172	0.0
66-67	0.0	5.422202325752021E-4	0.0	0.13703769159224835	0.0
68-69	0.0	5.591646148431773E-4	0.0	0.14318003016438932	0.0
70-71	0.0	5.761089971111523E-4	0.0	0.14830570580045177	0.0
72-73	0.0	5.761089971111523E-4	0.0	0.1529908274975469	0.0
74-75	0.0	5.761089971111523E-4	0.0	0.1563797039511419	0.0
76-77	0.0	5.845811882451398E-4	0.0	0.1595567756263872	0.0
78-79	0.0	5.930533793791274E-4	0.0	0.16193746133503772	0.0
80-81	0.0	5.930533793791274E-4	0.0	0.16269995853709657	0.0
82-83	0.0	5.930533793791274E-4	0.0	0.16306426275585806	0.0
84-85	0.0	5.930533793791274E-4	0.0	0.1633014841076097	0.0
86-87	0.0	5.930533793791274E-4	0.0	0.1633862060189496	0.0
88	0.0	6.608309084510276E-4	0.0	0.16339467821008358	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9215	0.0	53.050606	1
GTATCAA	17460	0.0	40.02953	1
ATCAACG	21530	0.0	31.873594	3
TATCAAC	21835	0.0	31.777151	2
CAACGCA	21895	0.0	31.2074	5
TCAACGC	22030	0.0	30.997015	4
AACGCAG	23065	0.0	29.788946	6
ACGCAGA	25005	0.0	27.376581	7
CGCAGAG	26225	0.0	26.086924	8
GCAGAGT	29610	0.0	23.175903	9
GTGGTAT	4545	0.0	22.458652	1
TGGTATC	4425	0.0	21.16128	2
TACATGG	21455	0.0	20.780151	2
GAGTACT	17535	0.0	20.698202	12-13
GTACATG	22005	0.0	20.644129	1
ACATGGG	21155	0.0	20.456053	3
GTACTAG	2005	0.0	20.40608	1
CTCGATA	1015	0.0	20.170818	94
CAGAGTA	29280	0.0	19.886248	10-11
AGAGTAC	28090	0.0	18.829264	10-11
>>END_MODULE