FastQCFastQC Report
Fri 27 May 2016
SRR2139386_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139386_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6122747
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC70100.048.4537541
GGTATCA69750.046.243651
TATACAC93500.040.3512653
GTATCAA128750.039.4724581
TTATACA104100.034.765192
ATCAACG148750.033.156793
TCAACGC152250.032.3945664
CAACGCA152650.032.3096775
TATCAAC156900.031.6544152
AACGCAG159450.031.0659186
TACACAT125700.029.9125735
ACACATC123850.028.9778446
ACATCTC124200.028.6550968
ACGCAGA178850.027.6722557
ATACACA145900.026.885264
CGCAGAG188550.026.2259648
CACATCT138550.025.7180847
CATCTCC153450.022.8584659
GCAGAGT222450.022.2869789
GTGGTAT39950.022.2726291
Produced by FastQC (version 0.11.5)