##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139386_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6122747
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.723648225216557	30.0	18.0	33.0	18.0	34.0
2	31.607033248311584	33.0	32.0	33.0	27.0	34.0
3	32.38844623173226	33.0	33.0	33.0	31.0	34.0
4	32.819334524193145	33.0	33.0	34.0	31.0	34.0
5	33.10029975107578	33.0	33.0	34.0	32.0	34.0
6	36.275779809291485	38.0	36.0	38.0	33.0	38.0
7	37.015853831621655	38.0	38.0	38.0	35.0	38.0
8	36.557472487430886	38.0	37.0	38.0	34.0	38.0
9	37.14632223085488	38.0	38.0	38.0	36.0	38.0
10-11	37.31752030583658	38.0	38.0	38.0	36.5	38.0
12-13	37.38571191982945	38.0	38.0	38.0	37.0	38.0
14-15	37.36424606471572	38.0	38.0	38.0	36.5	38.0
16-17	37.43021906670323	38.0	38.0	38.0	37.0	38.0
18-19	37.44814410917191	38.0	38.0	38.0	37.0	38.0
20-21	37.478484666820336	38.0	38.0	38.0	37.0	38.0
22-23	37.493503542555935	38.0	38.0	38.0	37.0	38.0
24-25	37.51262106840245	38.0	38.0	38.0	37.0	38.0
26-27	37.47765772263526	38.0	38.0	38.0	37.0	38.0
28-29	37.451326026932236	38.0	38.0	38.0	37.0	38.0
30-31	37.46755864203442	38.0	38.0	38.0	37.0	38.0
32-33	37.44740344758591	38.0	38.0	38.0	37.0	38.0
34-35	37.420259104631725	38.0	38.0	38.0	37.0	38.0
36-37	37.38864963266187	38.0	38.0	38.0	37.0	38.0
38-39	37.3661217403035	38.0	38.0	38.0	37.0	38.0
40-41	37.40063793149514	38.0	38.0	38.0	37.0	38.0
42-43	37.37929648887696	38.0	38.0	38.0	37.0	38.0
44-45	37.35321157079471	38.0	38.0	38.0	37.0	38.0
46-47	37.3344970714773	38.0	38.0	38.0	37.0	38.0
48-49	37.288969992733925	38.0	38.0	38.0	37.0	38.0
50-51	37.21692462701371	38.0	38.0	38.0	37.0	38.0
52-53	37.207217335457344	38.0	38.0	38.0	37.0	38.0
54-55	37.14892146678371	38.0	38.0	38.0	36.5	38.0
56-57	37.12275104103941	38.0	38.0	38.0	36.0	38.0
58-59	37.122903146015744	38.0	38.0	38.0	36.0	38.0
60-61	37.12432533641672	38.0	38.0	38.0	36.0	38.0
62-63	37.13223016384508	38.0	38.0	38.0	36.0	38.0
64-65	37.13629346157809	38.0	38.0	38.0	36.0	38.0
66-67	37.131830997836786	38.0	38.0	38.0	36.0	38.0
68-69	37.12192192937369	38.0	38.0	38.0	36.0	38.0
70-71	37.11443134657003	38.0	38.0	38.0	36.0	38.0
72-73	37.117343543440086	38.0	38.0	38.0	36.0	38.0
74-75	37.10840140433084	38.0	38.0	38.0	36.0	38.0
76-77	37.105223291649146	38.0	38.0	38.0	36.0	38.0
78-79	37.08356089516755	38.0	38.0	38.0	36.0	38.0
80-81	37.0782113329623	38.0	38.0	38.0	36.0	38.0
82-83	37.02785100959997	38.0	38.0	38.0	36.0	38.0
84-85	37.00925502719318	38.0	38.0	38.0	36.0	38.0
86-87	37.0044519861428	38.0	38.0	38.0	36.0	38.0
88-89	36.98047344137665	38.0	38.0	38.0	36.0	38.0
90-91	36.981957174193965	38.0	38.0	38.0	36.0	38.0
92-93	36.981339703865885	38.0	38.0	38.0	36.0	38.0
94-95	36.98254460740178	38.0	38.0	38.0	35.5	38.0
96-97	36.99795346463931	38.0	38.0	38.0	35.5	38.0
98-99	36.98143202621107	38.0	38.0	38.0	35.0	38.0
100	35.422002972042094	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	22.0
21	73.0
22	286.0
23	859.0
24	2025.0
25	4355.0
26	7891.0
27	13587.0
28	21240.0
29	31374.0
30	42245.0
31	55059.0
32	71357.0
33	97964.0
34	149629.0
35	289227.0
36	934237.0
37	4401314.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.14130667166224	18.241191412939322	13.109164889550392	23.50833702584804
2	14.721798911205955	19.769403025299027	43.02696504784912	22.481833015645904
3	18.47139854055704	25.873582560246245	30.32085108203883	25.334167817157887
4	12.61342335392921	18.203103933577527	38.194473820329335	30.988998892163927
5	13.885205447816151	37.36371925869222	35.34799004433794	13.40308524915369
6	30.63417449716606	37.403782975190715	18.319946912717445	13.642095614925784
7	27.14053022279052	32.60190238139841	22.179930021606314	18.07763737420475
8	24.868331159200274	36.533781323971084	20.73570898813882	17.862178528689817
9	25.68819191777808	17.316459425810017	20.72197332341186	36.27337533300004
10-11	24.628398005013107	26.569454854169216	28.453225325168592	20.34892181564909
12-13	25.526638614987686	23.791461577621938	28.406702089764615	22.275197717625765
14-15	22.660523127935875	25.299134522461898	25.999596259652733	26.040746089949497
16-17	22.510892578118938	28.61595457071801	26.59587273490151	22.27728011626154
18-19	22.37360942727178	27.435838848151	28.294767038389796	21.895784686187426
20-21	23.172875669946	26.683886752484	28.24105234704652	21.902185230523486
22-23	22.880989682693162	26.8194445170361	27.991550222391798	22.308015577878944
24-25	22.808690347171236	26.998805167101015	28.20130688421391	21.991197601513836
26-27	22.735132484317205	27.221716447083296	28.144502416639533	21.898648651959967
28-29	22.247782148702232	27.018620726450422	28.35909112115152	22.374506003695828
30-31	22.922751456015334	26.681470784015804	28.476020928022717	21.919756831946145
32-33	22.473213756886565	27.24420470062921	28.143661803604985	22.13891973887924
34-35	22.47816495726601	27.336229481341523	28.48525382762459	21.700351733767878
36-37	22.837285479066573	26.964463853087278	28.146609831595075	22.05164083625107
38-39	22.415894928849863	26.890437707274984	28.473101809089123	22.220565554786035
40-41	22.727224632887033	26.786805836216594	28.471955099566248	22.01401443133013
42-43	22.94842939031828	26.668796356152686	28.36326547099016	22.01950878253887
44-45	22.623326489350955	27.211028096894996	28.081190766859915	22.084454646894137
46-47	22.959520474265794	26.765451059188557	27.599982069925083	22.675046396620573
48-49	22.817384690379548	26.647173128305685	27.95917622011221	22.57626596120256
50-51	22.412671384165588	27.18199871089691	27.989946619593592	22.41538328534391
52-53	22.71627707987953	27.17835340071591	28.027213901211347	22.07815561819321
54-55	22.763278336755384	27.08917218256852	27.706814247380308	22.440735233295786
56-57	22.974463753659492	27.423382123011862	27.358047868995282	22.24410625433336
58-59	22.573997765305847	27.78166820968672	27.93502974756358	21.70930427744385
60-61	22.95987707231785	28.14162532726713	27.08996356867247	21.808534031742553
62-63	22.550908898788315	28.471651472099946	27.119582977075098	21.85785665203665
64-65	22.52899238818807	28.404690512072744	27.12334189441078	21.942975205328406
66-67	22.799526666257094	28.341180718254805	26.89128249349618	21.96801012199192
68-69	22.753924839262798	28.041540479348825	27.25251360658222	21.952021074806154
70-71	22.87748211668317	27.436932953165694	27.33726699053301	22.34831793961813
72-73	23.03162727516835	27.25840628247292	27.420972496710526	22.288993945648205
74-75	22.65050609566386	27.369686729638403	27.538860201221187	22.440946973476546
76-77	22.749277111170226	27.28745830406084	27.434078280308817	22.52918630446012
78-79	22.867296246142217	27.347066511305663	27.317434417711155	22.468202824840965
80-81	22.812983634398336	27.228261323281355	27.38033098531918	22.57842405700113
82-83	22.89884354731167	27.24021732069602	27.482867265319662	22.37807186667265
84-85	23.113273607729536	27.17163669543367	27.290302875410237	22.42478682142655
86-87	22.711126752782253	27.272729132862583	27.462315053721635	22.55382906063353
88-89	22.86735458818995	27.198697657405575	27.437240033912158	22.496707720492314
90-91	22.866397523810537	27.243858736235723	27.405298503277926	22.484445236675814
92-93	22.99520701444439	27.23870102334437	27.37823215189379	22.38785981031745
94-95	22.75048674067095	27.46334415192646	27.374301735580296	22.41186737182229
96-97	22.78026386827783	27.452608892317183	27.546044089015503	22.221083150389486
98-99	23.98126192139682	29.38095118178853	27.591990578253263	19.045796318561393
100	22.91879011426853	30.285520189124547	21.461384511785848	25.334305184821083
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	482.0
1	498.0
2	748.0
3	1099.0
4	1583.5
5	2437.0
6	3042.5
7	3538.0
8	4242.5
9	4751.5
10	5069.5
11	5370.5
12	6126.5
13	7831.5
14	11024.0
15	14826.5
16	18118.0
17	20734.5
18	21376.5
19	19924.0
20	18062.5
21	16713.0
22	17194.0
23	20026.0
24	24801.0
25	32384.5
26	43279.5
27	55973.5
28	66043.0
29	76150.0
30	85508.0
31	94259.0
32	107424.0
33	122095.0
34	137771.5
35	152989.0
36	171176.5
37	187949.0
38	194866.5
39	201460.0
40	214912.5
41	226550.0
42	235376.5
43	251759.5
44	273283.0
45	285133.0
46	281429.5
47	273656.5
48	267475.0
49	263076.0
50	260966.0
51	257324.0
52	247094.0
53	233093.0
54	219085.0
55	202983.0
56	186429.5
57	170006.5
58	154539.5
59	137356.0
60	114261.5
61	90315.5
62	70203.0
63	53186.5
64	39631.0
65	28873.5
66	20912.0
67	15925.0
68	11875.5
69	8500.5
70	6133.0
71	4301.5
72	2943.0
73	1972.5
74	1269.0
75	837.0
76	546.0
77	322.5
78	187.0
79	119.0
80	84.5
81	56.0
82	37.0
83	24.0
84	14.0
85	9.5
86	7.5
87	5.0
88	3.0
89	2.5
90	3.0
91	3.0
92	2.5
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.006157366946568264
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	4.083167590755545E-5
22-23	0.0
24-25	3.348461026894594E-4
26-27	0.0030302027908086027
28-29	0.006682990702064514
30-31	0.007179386036568424
32-33	0.0022363865498306245
34-35	7.143835461210944E-4
36-37	2.471901483849502E-5
38-39	5.881134810519776E-4
40-41	9.164433599679546E-5
42-43	9.218217242038456E-5
44-45	1.3501734846348991E-4
46-47	1.7011453386278648E-5
48-49	7.372820559950291E-4
50-51	3.7137362293796953E-4
52-53	0.0
54-55	0.00812574805260056
56-57	0.0626110262640726
58-59	0.07402258920753674
60-61	0.04577175557918406
62-63	0.009298143196113141
64-65	1.0046416269787558E-4
66-67	9.224619957188539E-6
68-69	6.531713990800734E-4
70-71	0.002891523913422482
72-73	0.013074809446138246
74-75	0.01170573520178244
76-77	0.017582337041510594
78-79	0.006298022420959819
80-81	0.00707574264490465
82-83	0.042913187204363414
84-85	0.02617874776026832
86-87	0.009442085287093099
88-89	0.0021801673789924444
90-91	2.088482966698408E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	30.0
20-21	121.0
22-23	313.0
24-25	388.0
26-27	1249.0
28-29	3386.0
30-31	10616.0
32-33	13516.0
34-35	18126.0
36-37	29708.0
38-39	35467.0
40-41	34167.0
42-43	39067.0
44-45	46225.0
46-47	47039.0
48-49	43883.0
50-51	39824.0
52-53	38936.0
54-55	42646.0
56-57	45122.0
58-59	45699.0
60-61	52474.0
62-63	48063.0
64-65	52477.0
66-67	60298.0
68-69	61613.0
70-71	76023.0
72-73	48039.0
74-75	46141.0
76-77	48663.0
78-79	49602.0
80-81	49800.0
82-83	47175.0
84-85	47121.0
86-87	48331.0
88-89	50308.0
90-91	51148.0
92-93	49932.0
94-95	59332.0
96-97	188159.0
98-99	416238.0
100-101	4036282.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.42215430742385
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.4753963722144	74.64230322880783
2	7.116779452191476	11.873941472652415
3	1.7909192766509332	4.482070327467421
4	0.7105176076960522	2.3709163802944686
5	0.3458930655208141	1.4427572342872612
6	0.1737162507101463	0.8695070323469372
7	0.10549824439260508	0.616062357601755
8	0.06673345484271358	0.44536388538850835
9	0.043110368711671176	0.3236723847823473
>10	0.1648027511371656	2.194188615361281
>50	0.004472465963609008	0.257847825329242
>100	0.0019166817635665133	0.26580249784660703
>500	1.7429157527413242E-4	0.10535523620621157
>1k	6.971662959106275E-5	0.1102115216276669
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.633253831980972E-5	0.0
2	0.0	0.0	0.0	1.633253831980972E-5	0.0
3	0.0	0.0	0.0	1.633253831980972E-5	0.0
4	0.0	0.0	0.0	1.633253831980972E-5	0.0
5	0.0	0.0	0.0	1.633253831980972E-5	0.0
6	0.0	0.0	0.0	1.633253831980972E-5	0.0
7	0.0	0.0	0.0	3.266507663961944E-5	0.0
8	0.0	0.0	0.0	3.266507663961944E-5	0.0
9	0.0	0.0	0.0	8.16626915990486E-5	0.0
10-11	0.0	1.633253831980972E-5	0.0	8.16626915990486E-5	0.0
12-13	0.0	1.633253831980972E-5	0.0	8.16626915990486E-5	0.0
14-15	1.633253831980972E-5	1.633253831980972E-5	0.0	8.16626915990486E-5	0.0
16-17	6.533015327923888E-5	1.633253831980972E-5	0.0	8.16626915990486E-5	0.0
18-19	8.16626915990486E-5	1.633253831980972E-5	0.0	8.982896075895345E-5	0.0
20-21	8.16626915990486E-5	1.633253831980972E-5	0.0	9.799522991885831E-5	0.0
22-23	8.16626915990486E-5	1.633253831980972E-5	0.0	1.1432776823866804E-4	0.0
24-25	8.982896075895345E-5	1.633253831980972E-5	0.0	1.1432776823866804E-4	0.0
26-27	1.1432776823866804E-4	1.633253831980972E-5	0.0	1.3882657571838262E-4	0.0
28-29	1.1432776823866804E-4	1.633253831980972E-5	0.0	3.021519589164798E-4	0.0
30-31	1.3066030655847777E-4	1.633253831980972E-5	0.0	0.001396432026343731	0.0
32-33	1.633253831980972E-4	1.633253831980972E-5	0.0	0.003617657237837853	0.0
34-35	1.796579215179069E-4	1.633253831980972E-5	0.0	0.005928711410090928	0.0
36-37	1.796579215179069E-4	1.633253831980972E-5	0.0	0.009709694031126878	0.0
38-39	1.796579215179069E-4	1.633253831980972E-5	0.0	0.0152872558673419	0.0
40-41	1.796579215179069E-4	1.633253831980972E-5	0.0	0.021289463699871967	0.0
42-43	1.796579215179069E-4	1.633253831980972E-5	0.0	0.02700585211180537	0.0
44-45	1.8782419067781177E-4	1.633253831980972E-5	0.0	0.03341637340233068	0.0
46-47	1.9599045983771662E-4	1.633253831980972E-5	0.0	0.040692519223805915	0.0
48-49	1.9599045983771662E-4	1.633253831980972E-5	0.0	0.04792783369948162	0.0
50-51	1.9599045983771662E-4	1.633253831980972E-5	0.0	0.05442001768160598	0.0
52-53	1.9599045983771662E-4	1.633253831980972E-5	0.0	0.060667213588933205	0.0
54-55	2.0415672899762148E-4	1.633253831980972E-5	0.0	0.0670532360719788	0.0
56-57	2.6132061311695553E-4	1.633253831980972E-5	0.0	0.07383940574385975	0.0
58-59	3.103182280763847E-4	1.633253831980972E-5	0.0	0.08064190795406048	0.0
60-61	3.266507663961944E-4	1.633253831980972E-5	0.0	0.08701976416794618	0.0
62-63	3.266507663961944E-4	1.633253831980972E-5	0.0	0.0925891597350013	0.0
64-65	3.266507663961944E-4	1.633253831980972E-5	0.0	0.09840354337685356	0.0
66-67	3.3481703555609925E-4	1.633253831980972E-5	0.0	0.10393210759810914	0.0
68-69	3.429833047160041E-4	1.633253831980972E-5	0.0	0.10907685716884921	0.0
70-71	3.429833047160041E-4	1.633253831980972E-5	0.0	0.11340497982359879	0.0
72-73	3.429833047160041E-4	1.633253831980972E-5	0.0	0.11730845648203331	0.0
74-75	3.593158430358138E-4	1.633253831980972E-5	0.0	0.12066479310675421	0.0
76-77	3.593158430358138E-4	1.633253831980972E-5	0.0	0.12415179003803357	0.0
78-79	3.593158430358138E-4	1.633253831980972E-5	0.0	0.12632401763456827	0.0
80-81	3.593158430358138E-4	1.633253831980972E-5	0.0	0.12707531439727954	0.0
82-83	3.593158430358138E-4	1.633253831980972E-5	0.0	0.127548958008554	0.0
84-85	3.593158430358138E-4	1.633253831980972E-5	0.0	0.12763878696931297	0.0
86-87	3.593158430358138E-4	1.633253831980972E-5	0.0	0.1277122833917521	0.0
88	3.593158430358138E-4	1.633253831980972E-5	0.0	0.12773678219923182	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	7010	0.0	48.453754	1
GGTATCA	6975	0.0	46.24365	1
TATACAC	9350	0.0	40.351265	3
GTATCAA	12875	0.0	39.472458	1
TTATACA	10410	0.0	34.76519	2
ATCAACG	14875	0.0	33.15679	3
TCAACGC	15225	0.0	32.394566	4
CAACGCA	15265	0.0	32.309677	5
TATCAAC	15690	0.0	31.654415	2
AACGCAG	15945	0.0	31.065918	6
TACACAT	12570	0.0	29.912573	5
ACACATC	12385	0.0	28.977844	6
ACATCTC	12420	0.0	28.655096	8
ACGCAGA	17885	0.0	27.672255	7
ATACACA	14590	0.0	26.88526	4
CGCAGAG	18855	0.0	26.225964	8
CACATCT	13855	0.0	25.718084	7
CATCTCC	15345	0.0	22.858465	9
GCAGAGT	22245	0.0	22.286978	9
GTGGTAT	3995	0.0	22.272629	1
>>END_MODULE