FastQCFastQC Report
Fri 27 May 2016
SRR2139385_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139385_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5717835
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73700.053.4239351
GTATCAA137500.038.95651
TCAACGC157450.032.9469874
ATCAACG160450.032.519723
CAACGCA162050.032.0117455
TATCAAC166550.031.7199842
AACGCAG166150.031.2738846
ACGCAGA187950.027.623467
CGCAGAG191600.027.074658
GCAGAGT227450.022.8072249
GTACATG174350.022.7567041
TACATGG173000.022.158972
ACATGGG172450.021.852153
GTGGTAT34300.021.0661091
GAGTACT137950.020.87207212-13
CAGAGTA222200.020.3669910-11
TGGTATC33650.019.5443172
CATGGGG132350.019.4505634
GTACTTT150750.018.94214-15
AGAGTAC208100.018.91944910-11
Produced by FastQC (version 0.11.5)