FastQCFastQC Report
Fri 27 May 2016
SRR2139384_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139384_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10817111
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123750.050.8364141
GTATCAA224400.039.229031
ATCAACG266750.032.2396133
TCAACGC268600.032.050434
CAACGCA271300.031.6989175
AACGCAG279800.030.8148336
TATCAAC287300.030.1487052
ACGCAGA317500.027.1002547
CGCAGAG323200.026.5949948
GCAGAGT388400.021.994149
GAGTACT233800.019.57231112-13
AGAGTAC356000.019.37703510-11
CAGAGTA383150.019.06981510-11
TACATGG290900.018.7425422
GTACATG305450.018.3122521
ACATGGG289650.018.1680363
GTGGTAT64950.018.148361
AGTACTT258900.017.13765312-13
GTACTTT268750.017.0687814-15
TGGTATC62200.016.892422
Produced by FastQC (version 0.11.5)