##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2139384_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10817111 Sequences flagged as poor quality 0 Sequence length 19-100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.177866992397504 33.0 31.0 34.0 30.0 34.0 2 32.401153505774325 34.0 31.0 34.0 31.0 34.0 3 32.44985088902203 34.0 31.0 34.0 31.0 34.0 4 36.08511144981317 37.0 35.0 37.0 35.0 37.0 5 35.95040699868939 37.0 35.0 37.0 35.0 37.0 6 35.97106353073385 37.0 35.0 37.0 35.0 37.0 7 35.92427377328383 37.0 35.0 37.0 35.0 37.0 8 35.920244324015904 37.0 35.0 37.0 35.0 37.0 9 37.69769608539655 39.0 37.0 39.0 35.0 39.0 10-11 37.58196264233583 39.0 37.0 39.0 35.0 39.0 12-13 37.55180930472101 39.0 37.0 39.0 35.0 39.0 14-15 38.82938933510066 40.0 38.0 41.0 35.0 41.0 16-17 38.81575833880229 40.0 38.0 41.0 35.0 41.0 18-19 38.80160451344171 40.0 38.0 41.0 35.0 41.0 20-21 38.79131931640764 40.0 38.0 41.0 34.0 41.0 22-23 38.71789888063958 40.0 38.0 41.0 34.0 41.0 24-25 38.66170160717712 40.0 38.0 41.0 34.0 41.0 26-27 38.50237111024744 40.0 38.0 41.0 34.0 41.0 28-29 38.37061842766538 40.0 38.0 41.0 34.0 41.0 30-31 38.23863203190764 40.0 38.0 41.0 34.0 41.0 32-33 38.14963767248707 40.0 38.0 41.0 33.5 41.0 34-35 38.21471915651598 40.0 38.0 41.0 34.0 41.0 36-37 38.18254495782465 40.0 38.0 41.0 33.5 41.0 38-39 38.12257196222858 40.0 38.0 41.0 33.0 41.0 40-41 38.05943416834702 40.0 37.5 41.0 33.0 41.0 42-43 38.034294808067756 40.0 37.5 41.0 33.0 41.0 44-45 38.06576380004316 40.0 38.0 41.0 33.0 41.0 46-47 38.0620475859464 40.0 37.5 41.0 33.0 41.0 48-49 37.99304641656019 40.0 37.0 41.0 33.0 41.0 50-51 37.92401118152502 40.0 37.0 41.0 33.0 41.0 52-53 37.71330466867167 40.0 37.0 41.0 32.0 41.0 54-55 37.614097517737534 40.0 37.0 41.0 32.0 41.0 56-57 37.57081806018725 39.5 36.5 41.0 32.0 41.0 58-59 37.431893815563704 39.0 36.0 41.0 32.0 41.0 60-61 37.26589468035128 39.0 36.0 41.0 31.0 41.0 62-63 37.0414130790936 39.0 35.0 40.5 31.0 41.0 64-65 36.77188846023519 38.5 35.0 40.0 31.0 41.0 66-67 36.50988878199864 38.0 35.0 40.0 31.0 41.0 68-69 36.239123839439856 37.5 35.0 40.0 30.0 41.0 70-71 35.95307486043236 37.0 34.0 39.5 30.0 41.0 72-73 35.644388265554404 36.5 34.0 39.0 30.0 41.0 74-75 35.282803308344945 36.0 34.0 39.0 29.5 40.0 76-77 33.918224975389606 34.5 32.0 37.0 28.5 39.0 78-79 34.631283615070046 35.0 34.0 37.0 29.0 39.0 80-81 34.55204873043088 35.0 34.0 37.0 29.5 39.0 82-83 34.2370934878978 35.0 34.0 37.0 29.0 39.0 84-85 34.01556448731813 35.0 34.0 36.0 29.0 37.5 86-87 33.747950134450484 35.0 33.0 36.0 29.0 37.0 88-89 33.6056164531563 35.0 33.0 35.5 29.0 37.0 90-91 33.39979387852655 35.0 33.0 35.0 29.0 36.0 92-93 33.27662278992291 35.0 33.0 35.0 29.0 36.0 94-95 33.25359251181061 35.0 33.0 35.0 29.0 36.0 96-97 33.27658083361776 35.0 33.0 35.0 29.0 36.0 98-99 33.36831783416682 35.0 33.0 35.0 29.5 35.0 100 32.08432429417743 34.0 31.0 35.0 26.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 0.0 15 0.0 16 0.0 17 0.0 18 2.0 19 3.0 20 41.0 21 248.0 22 1248.0 23 4818.0 24 13425.0 25 29422.0 26 54733.0 27 89476.0 28 130763.0 29 176136.0 30 226311.0 31 282137.0 32 349532.0 33 442973.0 34 583710.0 35 796223.0 36 1214590.0 37 2142904.0 38 2965270.0 39 1276308.0 40 36837.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.448757528696895 21.253262539323114 15.010551338522827 23.287428593457165 2 13.997609897873842 21.88334759622971 42.34461493461609 21.774427571280352 3 17.47919569282408 27.10090522321533 31.422678384274693 23.997220699685894 4 11.741323538234932 19.096263318366614 40.865236568248214 28.29717657515024 5 12.270087641700265 38.752787135123235 36.65728307678455 12.319842146391952 6 28.193627670086773 39.733760705608 19.69754216259776 12.375069461707474 7 25.27245028732718 33.48198978451825 24.213942151467243 17.031617776687323 8 23.515446961762713 37.467712035126574 22.00550590633673 17.01133509677399 9 25.085404041800068 17.230081118701655 21.995762084719296 35.68875275477898 10-11 23.115770005503318 27.575292515718846 30.128978060777968 19.17995941799987 12-13 24.200579064040298 25.09865157157026 29.9460872685877 20.754682095801734 14-15 21.23579946623456 26.68293317873876 27.798753290042043 24.282514064984635 16-17 20.65484669488682 29.736988841010948 28.443299926425823 21.164864537676408 18-19 20.587081212522037 29.000440182409225 30.152458053362054 20.26002055170669 20-21 21.619387988393264 28.19461928786656 29.995508846822926 20.190483876917256 22-23 21.11410052021143 28.172589771190925 29.820702643256936 20.892607065340705 24-25 20.907419249128107 28.233055503394272 29.98769692400905 20.87182832346857 26-27 20.938566517376884 28.420286171007312 29.767484032669966 20.873663278945838 28-29 20.81977514670608 28.616633894091372 29.787616959822756 20.775973999379797 30-31 21.297872709866887 28.32216921778649 29.688834765456335 20.69112330689028 32-33 21.051147126218098 28.428157167686834 29.620339356434407 20.900356349660665 34-35 21.122818867625195 28.53710848570539 29.640346413857895 20.69972623281153 36-37 21.095317745592205 28.498191277178243 29.59849475253803 20.807996224691525 38-39 20.901814926259874 28.637750115408455 29.67833414750563 20.78210081082604 40-41 21.266008736420126 28.527149450440426 29.44860590733255 20.758235905806902 42-43 21.14455473825695 28.548401569701916 29.303687897481723 21.003355794559415 44-45 21.27939811958163 28.762803040825176 29.036985476550196 20.920813363043 46-47 21.2332180198794 28.65816010450632 28.817386447949815 21.291235427664464 48-49 21.2282802156873 28.72803567145598 28.967696172046963 21.075987940809757 50-51 21.191378905865704 28.83943302220614 28.79727437291524 21.17191369901292 52-53 21.30449248207964 28.84728426455733 28.659496119278984 21.188727134084044 54-55 21.383274364294195 28.703434400728767 28.693588262089925 21.219702972887113 56-57 21.37378413396753 28.82727038999552 28.614960147000417 21.18398532903653 58-59 21.29895894293536 28.89005155778864 28.747310368112895 21.063679131163106 60-61 21.358775454151715 28.813613616080946 28.713908774104347 21.113702155662992 62-63 21.286886227880352 28.86090978502282 28.61942986129081 21.232774125806017 64-65 21.253845244824806 28.84064112360235 28.65521900210326 21.250294629469586 66-67 21.38112381748625 28.907999931272155 28.469383184461073 21.241493066780524 68-69 21.37790027991592 28.854468798671444 28.574243159484137 21.1933877619285 70-71 21.29906980431371 28.561958579113938 28.700545636583808 21.438425979988548 72-73 21.338452077947693 28.639119093990708 28.7042805820587 21.318148246002906 74-75 21.225121214461023 28.80076781891795 28.580138043606034 21.39397292301499 76-77 21.248112633644052 28.720608848140678 28.56396237715097 21.467316141064295 78-79 21.338972356658736 28.773546524138098 28.478479908383353 21.40900121081981 80-81 21.26300863291189 28.65981656780821 28.567882784712985 21.50929201456692 82-83 21.38773167627631 28.658309942195046 28.662985018115133 21.290973363413507 84-85 21.558673528595413 28.618216032059074 28.563117546297722 21.259992893047787 86-87 21.23248404916773 28.699608533079918 28.598916563395594 21.468990854356754 88-89 21.398546769242106 28.648997587324722 28.59322850874187 21.359227134691306 90-91 21.442153741894256 28.564488908964456 28.694613645621015 21.298743703520277 92-93 21.411999956208476 28.56737412395414 28.67271830728636 21.34790761255103 94-95 21.221674199715316 28.72067491775141 28.70214185463115 21.355509027902123 96-97 21.191984961250697 28.86347705596647 28.864386032082457 21.080151950700376 98-99 21.331563163787017 29.628710702427348 27.98632466301229 21.05340147077335 100 19.636950648191757 29.592172124036388 28.92790570854919 21.842971519222665 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1233.0 1 1165.5 2 1993.5 3 3121.0 4 4342.5 5 7145.5 6 9498.0 7 11066.5 8 12149.0 9 12029.5 10 11193.0 11 10328.5 12 11085.5 13 14881.0 14 24184.0 15 37606.5 16 49142.5 17 56499.0 18 59429.0 19 58014.5 20 54031.0 21 49708.0 22 47898.5 23 51412.0 24 60437.0 25 75214.5 26 97104.5 27 122105.0 28 147477.0 29 174196.5 30 200642.0 31 228012.5 32 259214.5 33 291598.5 34 323616.5 35 353819.0 36 384932.0 37 411659.5 38 425075.0 39 433717.5 40 449128.5 41 461374.0 42 462493.5 43 461837.5 44 456955.5 45 450922.5 46 444515.5 47 433278.0 48 419415.5 49 402550.5 50 380645.5 51 351793.0 52 320804.0 53 290316.5 54 261076.0 55 232642.5 56 203971.0 57 176650.5 58 151837.5 59 129445.5 60 106528.0 61 82985.5 62 61094.0 63 44344.0 64 32689.5 65 23697.5 66 17233.0 67 12907.0 68 9704.0 69 6915.5 70 4797.5 71 3424.5 72 2464.5 73 1799.5 74 1250.0 75 799.0 76 526.0 77 346.5 78 223.5 79 135.5 80 86.0 81 65.5 82 57.0 83 40.5 84 26.0 85 19.5 86 14.5 87 12.0 88 8.5 89 5.0 90 3.5 91 3.0 92 2.5 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.001261889611745687 18-19 4.1600756431176494E-5 20-21 0.0 22-23 0.006758537785265075 24-25 0.00829476736575897 26-27 0.021788491513958372 28-29 0.016458007327214058 30-31 0.0020002632753931453 32-33 0.0062394847761670594 34-35 0.033510710752982606 36-37 0.005601420676602303 38-39 0.0027241997756453553 40-41 0.0076697527087353145 42-43 0.007267306324289944 44-45 0.008487665976827444 46-47 0.005144182807172965 48-49 0.001073073849133066 50-51 0.0025959321455906034 52-53 9.567689939920196E-4 54-55 0.005395778937157033 56-57 9.014186730622807E-4 58-59 0.0012899746721991815 60-61 7.286954039029024E-4 62-63 0.0013021722050507477 64-65 2.962952574339122E-4 66-67 0.005616100361650049 68-69 0.004041168366213575 70-71 0.0016638816337729164 72-73 0.001517831330689811 74-75 6.468396560381744E-4 76-77 6.667508824319707E-4 78-79 6.974422262107881E-4 80-81 9.842697549058946E-4 82-83 0.00726422507815345 84-85 7.312498012908148E-4 86-87 1.0166571787581363E-4 88-89 4.5437258151403547E-4 90-91 2.9035832847394016E-4 92-93 0.002306226411193834 94-95 0.003402416710591 96-97 5.210688395623253E-5 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 157.0 20-21 698.0 22-23 1485.0 24-25 3072.0 26-27 4026.0 28-29 6403.0 30-31 11518.0 32-33 15460.0 34-35 20823.0 36-37 26784.0 38-39 32788.0 40-41 37941.0 42-43 50541.0 44-45 55414.0 46-47 54123.0 48-49 45985.0 50-51 40665.0 52-53 38982.0 54-55 41996.0 56-57 45088.0 58-59 47232.0 60-61 48476.0 62-63 49425.0 64-65 54246.0 66-67 59057.0 68-69 62041.0 70-71 64109.0 72-73 64963.0 74-75 68407.0 76-77 68297.0 78-79 75789.0 80-81 81412.0 82-83 82304.0 84-85 89037.0 86-87 97788.0 88-89 112450.0 90-91 126417.0 92-93 143854.0 94-95 178709.0 96-97 434928.0 98-99 205621.0 100-101 8068600.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.21383601993742 #Duplication Level Percentage of deduplicated Percentage of total 1 87.15864183820538 70.7848764596847 2 8.55060384570305 13.888546771927473 3 2.343566717286991 5.709901292385863 4 0.9029878069475573 2.9334041472576726 5 0.41042544432980804 1.6666112367105488 6 0.22281117990394167 1.0857210376876496 7 0.132243937342858 0.7518026209395624 8 0.07411072258425642 0.4815052857021499 9 0.051968655995667475 0.3798516515587809 >10 0.1486764831070922 1.7547082597665846 >50 0.002389923890270982 0.13074125232734055 >100 0.0013561462946670134 0.20355447176708769 >500 9.877388140268688E-5 0.05875124249178416 >1k 1.1852452713118567E-4 0.17002426979276475 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 9.244612540261443E-6 0.0 3 0.0 0.0 0.0 9.244612540261443E-6 0.0 4 0.0 1.8489225080522886E-5 0.0 9.244612540261443E-6 0.0 5 0.0 2.7733837620784328E-5 0.0 9.244612540261443E-6 0.0 6 0.0 2.7733837620784328E-5 0.0 9.244612540261443E-6 0.0 7 0.0 2.7733837620784328E-5 0.0 9.244612540261443E-6 0.0 8 0.0 2.7733837620784328E-5 0.0 9.244612540261443E-6 0.0 9 0.0 4.6223062701307214E-5 0.0 2.7733837620784328E-5 0.0 10-11 0.0 4.6223062701307214E-5 0.0 3.697845016104577E-5 0.0 12-13 0.0 4.6223062701307214E-5 0.0 7.395690032209155E-5 0.0 14-15 0.0 5.5467675241568656E-5 0.0 8.320151286235299E-5 0.0 16-17 0.0 5.5467675241568656E-5 0.0 1.0169073794287587E-4 0.0 18-19 0.0 6.008998151169938E-5 0.0 1.294245755636602E-4 0.0 20-21 0.0 7.395690032209155E-5 0.0 1.3866918810392164E-4 0.0 22-23 0.0 1.0169073794287587E-4 0.0 2.0338147588575174E-4 0.0 24-25 0.0 1.1093535048313731E-4 0.0 4.344967893922878E-4 0.0 26-27 0.0 1.1093535048313731E-4 0.0 0.0011185981173716347 0.0 28-29 0.0 1.1093535048313731E-4 0.0 0.003152412876229152 0.0 30-31 0.0 1.294245755636602E-4 0.0 0.0033927728022759493 0.0 32-33 0.0 1.294245755636602E-4 0.0 0.003984428004852682 0.0 34-35 0.0 1.4329149437405236E-4 0.0 0.0046038170450501984 0.0 36-37 0.0 1.5715841318444455E-4 0.0 0.005967397394738762 0.0 38-39 4.622306270130722E-6 1.6640302572470597E-4 0.0 0.009582040897980985 0.0 40-41 9.244612540261443E-6 2.126260884260132E-4 0.0 0.013293752832895956 0.0 42-43 9.244612540261443E-6 2.2649300723640535E-4 0.0 0.01615033810783674 0.0 44-45 9.244612540261443E-6 2.4960453858705896E-4 0.0 0.019760359304808836 0.0 46-47 9.244612540261443E-6 2.6809376366758187E-4 0.0 0.023670830409339427 0.0 48-49 9.244612540261443E-6 2.6809376366758187E-4 0.0 0.027659880720462236 0.0 50-51 9.244612540261443E-6 2.8658298874810473E-4 0.0 0.03169053178801623 0.0 52-53 9.244612540261443E-6 2.8658298874810473E-4 0.0 0.041928940176355775 0.0 54-55 9.244612540261443E-6 2.958276012883662E-4 0.0 0.05418729640474245 0.0 56-57 9.244612540261443E-6 3.0507221382862764E-4 0.0 0.06798025831481253 0.0 58-59 9.244612540261443E-6 3.0507221382862764E-4 0.0 0.0804512406316252 0.0 60-61 9.244612540261443E-6 3.0507221382862764E-4 0.0 0.08619214501912756 0.0 62-63 9.244612540261443E-6 3.143168263688891E-4 0.0 0.09353698968236529 0.0 64-65 9.244612540261443E-6 3.143168263688891E-4 0.0 0.09710078781663607 0.0 66-67 9.244612540261443E-6 3.143168263688891E-4 0.0 0.10009604227968077 0.0 68-69 9.244612540261443E-6 3.235614389091505E-4 0.0 0.10301271753613325 0.0 70-71 9.244612540261443E-6 3.2818374517928125E-4 0.0 0.10582770205464287 0.0 72-73 9.244612540261443E-6 3.3280605144941195E-4 0.0 0.10794009602009261 0.0 74-75 9.244612540261443E-6 3.3742835771954265E-4 0.0 0.10976128469052412 0.0 76-77 9.244612540261443E-6 3.6053988907019626E-4 0.0 0.11152238337944392 0.0 78-79 9.244612540261443E-6 3.6053988907019626E-4 0.0 0.11280276221627013 0.0 80-81 9.244612540261443E-6 3.6053988907019626E-4 0.0 0.11318641363669099 0.0 82-83 9.244612540261443E-6 3.6053988907019626E-4 0.0 0.11344988509408843 0.0 84-85 9.244612540261443E-6 3.6053988907019626E-4 0.0 0.11356082044457157 0.0 86-87 9.244612540261443E-6 3.697845016104577E-4 0.0 0.11368562271386509 0.0 88 9.244612540261443E-6 3.697845016104577E-4 0.0 0.11369024502013522 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12375 0.0 50.836414 1 GTATCAA 22440 0.0 39.22903 1 ATCAACG 26675 0.0 32.239613 3 TCAACGC 26860 0.0 32.05043 4 CAACGCA 27130 0.0 31.698917 5 AACGCAG 27980 0.0 30.814833 6 TATCAAC 28730 0.0 30.148705 2 ACGCAGA 31750 0.0 27.100254 7 CGCAGAG 32320 0.0 26.594994 8 GCAGAGT 38840 0.0 21.99414 9 GAGTACT 23380 0.0 19.572311 12-13 AGAGTAC 35600 0.0 19.377035 10-11 CAGAGTA 38315 0.0 19.069815 10-11 TACATGG 29090 0.0 18.742542 2 GTACATG 30545 0.0 18.312252 1 ACATGGG 28965 0.0 18.168036 3 GTGGTAT 6495 0.0 18.14836 1 AGTACTT 25890 0.0 17.137653 12-13 GTACTTT 26875 0.0 17.06878 14-15 TGGTATC 6220 0.0 16.89242 2 >>END_MODULE