FastQCFastQC Report
Fri 27 May 2016
SRR2139383_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139383_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7852839
Sequences flagged as poor quality0
Sequence length19-100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96150.046.655891
GTATCAA165900.035.9562571
ATCAACG184200.031.3324723
TCAACGC186600.030.9530754
CAACGCA189900.030.368825
AACGCAG197950.029.2227766
TATCAAC203000.028.8210982
CGCAGAG223300.025.964428
ACGCAGA223450.025.9075897
GCAGAGT272000.021.1537939
GAGTACT176450.019.46089712-13
TACATGG187550.019.3650462
GTGGTAT47100.019.2543091
GTACATG203200.018.61021
AGAGTAC248800.018.5704310-11
ACATGGG189700.018.5653973
CAGAGTA274950.018.23727210-11
GTACTTT194850.017.58989714-15
GTGTAGC61050.017.5227471
TGGTATC48200.016.9882052
Produced by FastQC (version 0.11.5)