##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139383_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7852839
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19460910888406	33.0	31.0	34.0	31.0	34.0
2	32.41485913565782	34.0	31.0	34.0	31.0	34.0
3	32.466323962582194	34.0	31.0	34.0	31.0	34.0
4	36.09473847611036	37.0	35.0	37.0	35.0	37.0
5	35.96031498926694	37.0	35.0	37.0	35.0	37.0
6	35.981667394428946	37.0	35.0	37.0	35.0	37.0
7	35.93899913139694	37.0	35.0	37.0	35.0	37.0
8	35.93545901042922	37.0	35.0	37.0	35.0	37.0
9	37.715956738703035	39.0	37.0	39.0	35.0	39.0
10-11	37.60223869354765	39.0	37.0	39.0	35.0	39.0
12-13	37.57431719916835	39.0	37.0	39.0	35.0	39.0
14-15	38.85866258050115	40.0	38.0	41.0	35.0	41.0
16-17	38.84612679567224	40.0	38.0	41.0	35.0	41.0
18-19	38.83426223305992	40.0	38.0	41.0	35.0	41.0
20-21	38.82633580768325	40.0	38.0	41.0	34.5	41.0
22-23	38.753005746962344	40.0	38.0	41.0	34.0	41.0
24-25	38.69923653483133	40.0	38.0	41.0	34.0	41.0
26-27	38.53939420306565	40.0	38.0	41.0	34.0	41.0
28-29	38.41122050289936	40.0	38.0	41.0	34.0	41.0
30-31	38.283372432311964	40.0	38.0	41.0	34.0	41.0
32-33	38.19887892546878	40.0	38.0	41.0	34.0	41.0
34-35	38.261334641768855	40.0	38.0	41.0	34.0	41.0
36-37	38.22907032268714	40.0	38.0	41.0	34.0	41.0
38-39	38.172471229757846	40.0	38.0	41.0	33.0	41.0
40-41	38.11436167204863	40.0	38.0	41.0	33.0	41.0
42-43	38.091451207911774	40.0	38.0	41.0	33.0	41.0
44-45	38.13089491866634	40.0	38.0	41.0	33.0	41.0
46-47	38.130098590538324	40.0	38.0	41.0	33.0	41.0
48-49	38.06703048269375	40.0	38.0	41.0	33.0	41.0
50-51	38.00901313604278	40.0	37.0	41.0	33.0	41.0
52-53	37.809773822428	40.0	37.0	41.0	33.0	41.0
54-55	37.717064096347244	40.0	37.0	41.0	32.0	41.0
56-57	37.67950350952039	40.0	37.0	41.0	32.0	41.0
58-59	37.551917709308526	39.5	36.0	41.0	32.0	41.0
60-61	37.39776195080227	39.0	36.0	41.0	32.0	41.0
62-63	37.18461038106304	39.0	36.0	41.0	31.0	41.0
64-65	36.922458697877204	39.0	35.0	40.0	31.0	41.0
66-67	36.67102659937707	38.0	35.0	40.0	31.0	41.0
68-69	36.405423174373524	38.0	35.0	40.0	31.0	41.0
70-71	36.11732721045604	37.0	35.0	40.0	30.0	41.0
72-73	35.80482601007518	37.0	34.0	39.0	30.0	41.0
74-75	35.44603088638756	36.0	34.0	39.0	30.0	40.5
76-77	34.05653702085397	35.0	32.5	37.5	28.5	39.0
78-79	34.76519159418767	35.0	34.0	37.5	29.5	39.0
80-81	34.67608052359866	35.0	34.0	37.0	30.0	39.0
82-83	34.347510918658955	35.0	34.0	37.0	29.5	39.0
84-85	34.11537717411349	35.0	34.0	36.0	29.0	38.0
86-87	33.83496308929824	35.0	33.5	36.0	29.0	37.0
88-89	33.683662212450656	35.0	33.0	36.0	29.0	37.0
90-91	33.470218315019565	35.0	33.0	35.0	29.0	36.5
92-93	33.33993317976771	35.0	33.0	35.0	29.0	36.0
94-95	33.30955857833459	35.0	33.0	35.0	29.0	36.0
96-97	33.326267455722835	35.0	33.0	35.0	29.0	36.0
98-99	33.41099646317854	35.0	33.0	35.0	30.0	35.0
100	32.145215395540916	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	1.0
19	2.0
20	18.0
21	163.0
22	937.0
23	3313.0
24	9226.0
25	20088.0
26	37936.0
27	61731.0
28	90155.0
29	122117.0
30	157995.0
31	197456.0
32	245328.0
33	311776.0
34	411999.0
35	558468.0
36	851022.0
37	1530088.0
38	2228693.0
39	984998.0
40	29328.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.104438662246864	21.37309831514437	15.07174666384985	23.450716358758918
2	13.73285508591224	22.107558807712728	42.59116989409817	21.56841621227686
3	17.292650466920307	27.20754876039099	31.55689299118446	23.942907781504243
4	11.537572589989429	19.351447291864766	41.27572206688562	27.835258051260187
5	12.105469117601928	38.83269477446309	36.873390629809165	12.188445478125809
6	27.766467643103343	40.14135524744618	19.951485061644586	12.140692047805896
7	24.962093327012052	33.64314485500085	24.463725793945347	16.931036024041752
8	23.181718102204822	37.64875352722754	22.315751029659463	16.853777340908174
9	24.91707776003048	17.39821738354753	22.227948389111248	35.45675646731074
10-11	22.776215073300243	27.808433357668484	30.403978739408764	19.011372829622513
12-13	23.85805311938778	25.318639539152656	30.229035639212775	20.594271702246793
14-15	20.92699213621978	26.91463558593268	28.092992865382826	24.065379412464715
16-17	20.35881849969778	29.943524840264445	28.78646440107659	20.91119225896118
18-19	20.271330052861806	29.249928799594592	30.45383167058977	20.02490947695383
20-21	21.267325731553175	28.519040945787253	30.28399991060275	19.929633412056823
22-23	20.7374885077275	28.46690585325311	30.055678098048244	20.73992754097114
24-25	20.609610300107704	28.49241052405632	30.25891670270694	20.639062473129034
26-27	20.671234819782168	28.730464735046567	29.986320264602327	20.61198018056894
28-29	20.505817177375768	28.9728369812828	30.02165445768144	20.49969138365999
30-31	20.926542615533844	28.64582615799171	30.03472992907063	20.392901297403814
32-33	20.738381287346797	28.76671817170976	29.84806225153755	20.646838289405892
34-35	20.772359276229952	28.893361849899296	29.884135296172865	20.450143577697887
36-37	20.79003899383059	28.80245615044941	29.867987105428078	20.539517750291928
38-39	20.621087333829703	28.94506636141515	29.90727586610972	20.52657043864543
40-41	21.01350649313795	28.831115208124626	29.687409807675053	20.46796849106237
42-43	20.866813818262727	28.82985599943222	29.56892948832766	20.734400693977392
44-45	20.96865252403599	29.105156168519176	29.329433058119335	20.596758249325504
46-47	20.8887637577204	28.971360276587006	29.080738792219822	21.05913717347277
48-49	20.954905381960376	28.999390902187734	29.23061650974126	20.815087206110636
50-51	20.940192967521597	29.11950555822747	29.0550755575574	20.88522591669353
52-53	20.994370589613442	29.15230141785926	28.922800249983695	20.9305277425436
54-55	21.103607482457264	28.99392562513429	28.98661705455278	20.915849837855664
56-57	21.107206788212977	29.115397613016984	28.848232180439055	20.929163418330983
58-59	21.05240969655633	29.192393817845584	28.989653825817214	20.765542659780873
60-61	21.08875509487503	29.181730431611836	28.912582905330947	20.81693156818219
62-63	21.020404199124247	29.132144403712296	28.906564541078712	20.94088685608475
64-65	20.957276142700568	29.113098354769534	28.93548083823451	20.994144664295384
66-67	21.108977281756886	29.195730390832793	28.720793682876145	20.97449864453418
68-69	21.12332161274454	29.175302532313403	28.78711187672663	20.914263978215434
70-71	21.023911485385664	28.85176476415847	28.94942882733136	21.17489492312451
72-73	21.025363395656882	28.908769707797816	28.984726598969957	21.08114029757534
74-75	20.976593848689134	29.005878657832717	28.82713349094206	21.19039400253609
76-77	20.967523508548453	29.005562075740915	28.830994948076068	21.195919467634564
78-79	21.073045134683362	29.032405182956317	28.78180450956635	21.112745172793968
80-81	20.979713570649096	28.931819158126	28.805459001356233	21.283008269868674
82-83	21.110825153992522	28.897737776480486	28.947687597198	21.043749472328987
84-85	21.321545574349297	28.865766209724512	28.783379015787645	21.029309200138545
86-87	20.964162967285816	28.93701363258039	28.870183472955723	21.22863992717807
88-89	21.147661913648307	28.89703019880388	28.853528124121315	21.1017797634265
90-91	21.15267099278063	28.826115258276065	28.953215202644166	21.06799854629914
92-93	21.140417693195275	28.805624205145268	28.96278754536667	21.091170556292788
94-95	20.940027082431886	28.978900646027373	28.95607687279145	21.12499539874929
96-97	20.8990226201029	29.152164359119325	29.10210682953679	20.846706191240987
98-99	21.07358642067366	29.818007638623833	28.254128741059414	20.854277199643093
100	19.420453553306295	29.806380160084917	29.172238060831717	21.600928225777068
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	818.0
1	787.0
2	1383.0
3	2201.5
4	3203.0
5	5292.5
6	7015.5
7	8025.5
8	8559.0
9	8395.5
10	7886.5
11	7461.5
12	8273.5
13	11272.0
14	18048.0
15	27653.5
16	36034.5
17	41443.5
18	43549.5
19	42584.5
20	39920.0
21	37137.5
22	36080.0
23	38837.0
24	45838.0
25	57328.0
26	74292.5
27	93487.5
28	112661.5
29	134399.5
30	155859.0
31	176710.0
32	199985.5
33	223544.0
34	248099.0
35	270063.5
36	291826.5
37	309874.0
38	319542.0
39	327027.0
40	337424.0
41	346693.5
42	348099.5
43	346068.5
44	341453.5
45	336726.0
46	331594.0
47	323462.0
48	310915.5
49	294132.0
50	275200.5
51	251335.5
52	224118.5
53	199069.5
54	175795.0
55	151375.0
56	128590.0
57	109448.5
58	92451.5
59	77167.5
60	61867.5
61	46364.0
62	32971.5
63	23342.5
64	16661.5
65	11877.5
66	8479.5
67	6206.0
68	4716.0
69	3499.0
70	2557.0
71	1773.0
72	1195.0
73	833.5
74	579.0
75	403.5
76	276.5
77	180.0
78	125.5
79	90.5
80	66.5
81	54.0
82	37.0
83	24.0
84	20.0
85	15.5
86	10.5
87	8.0
88	5.0
89	2.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0012033864440618228
18-19	5.730411638389633E-5
20-21	0.0
22-23	0.006883586015915411
24-25	0.008152186027790038
26-27	0.021081005147931767
28-29	0.015978055189617514
30-31	0.001887876465527886
32-33	0.0064302665687120605
34-35	0.03281667483619165
36-37	0.005422548919755826
38-39	0.002763951414362147
40-41	0.007480868484977738
42-43	0.00753055881991287
44-45	0.008558212014441055
46-47	0.0052157081423224744
48-49	0.0010064912766808997
50-51	0.0025110549192821395
52-53	9.421740666588152E-4
54-55	0.005396192087117191
56-57	0.0010639928815530334
58-59	0.0014455764989333999
60-61	9.122645453655306E-4
62-63	0.001480570902706796
64-65	4.3695834660423624E-4
66-67	0.005480214057985197
68-69	0.003959844002878454
70-71	0.0016696811368190987
72-73	0.001540968144055866
74-75	7.617524651508827E-4
76-77	6.607772232222695E-4
78-79	9.09262217477194E-4
80-81	9.818770760653675E-4
82-83	0.006737709071112477
84-85	6.762643276153715E-4
86-87	6.68226902235954E-5
88-89	4.5799351090767854E-4
90-91	3.118540675087994E-4
92-93	0.0025464025928860147
94-95	0.00317858815119657
96-97	5.627148063288373E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	170.0
20-21	441.0
22-23	1091.0
24-25	2157.0
26-27	2835.0
28-29	4655.0
30-31	8591.0
32-33	11742.0
34-35	15742.0
36-37	20524.0
38-39	25037.0
40-41	28605.0
42-43	38224.0
44-45	42161.0
46-47	41006.0
48-49	35238.0
50-51	31387.0
52-53	30010.0
54-55	32609.0
56-57	35131.0
58-59	36845.0
60-61	37144.0
62-63	38167.0
64-65	41547.0
66-67	44971.0
68-69	47639.0
70-71	48723.0
72-73	48830.0
74-75	51859.0
76-77	51928.0
78-79	57190.0
80-81	60963.0
82-83	61875.0
84-85	65802.0
86-87	72550.0
88-89	83008.0
90-91	91789.0
92-93	104099.0
94-95	128649.0
96-97	309854.0
98-99	143639.0
100-101	5818412.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.34023169755423
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.78122060439362	74.87828716166233
2	7.9216763043684955	13.362320298869278
3	1.931201236046918	4.886338791084004
4	0.6824574209080219	2.302344680123914
5	0.30157975071571413	1.2717653025326987
6	0.1385137552413482	0.7009369326212174
7	0.083252355288777	0.49150660540957886
8	0.045111837129124775	0.30437942366181703
9	0.029769659795013434	0.22597020042019175
>10	0.08166611427125434	1.004824789192039
>50	0.0018622015138391173	0.1119328366631141
>100	0.001385581314284761	0.21563190110146033
>500	2.0989441734014694E-4	0.11243042148171575
>1k	9.328459627839008E-5	0.1313306551764839
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.2734248085310294E-5	0.0
3	0.0	0.0	0.0	2.546849617062059E-5	0.0
4	0.0	0.0	0.0	2.546849617062059E-5	0.0
5	0.0	0.0	0.0	3.820274425593088E-5	0.0
6	0.0	0.0	0.0	3.820274425593088E-5	0.0
7	0.0	0.0	0.0	5.093699234124118E-5	0.0
8	0.0	0.0	0.0	6.367124042655146E-5	0.0
9	0.0	0.0	0.0	8.913973659717205E-5	0.0
10-11	0.0	0.0	0.0	1.2734248085310293E-4	0.0
12-13	0.0	0.0	0.0	1.2734248085310293E-4	0.0
14-15	0.0	0.0	0.0	1.2734248085310293E-4	0.0
16-17	0.0	0.0	0.0	1.3370960489575808E-4	0.0
18-19	0.0	0.0	0.0	1.655452251090338E-4	0.0
20-21	0.0	0.0	0.0	1.910137212796544E-4	0.0
22-23	0.0	0.0	0.0	2.992548300047919E-4	0.0
24-25	0.0	0.0	0.0	4.4569868298586026E-4	0.0
26-27	1.2734248085310294E-5	0.0	0.0	0.0011015124593793404	0.0
28-29	1.2734248085310294E-5	0.0	0.0	0.0036228935802707783	0.0
30-31	1.2734248085310294E-5	1.2734248085310294E-5	0.0	0.00389667991410495	0.0
32-33	1.2734248085310294E-5	1.2734248085310294E-5	0.0	0.0045270251943278095	0.0
34-35	1.2734248085310294E-5	1.2734248085310294E-5	0.0	0.005494828048811392	0.0
36-37	1.2734248085310294E-5	1.2734248085310294E-5	0.0	0.006959266578622075	0.0
38-39	2.546849617062059E-5	1.2734248085310294E-5	0.0	0.011218872563158368	0.0
40-41	2.546849617062059E-5	1.2734248085310294E-5	0.0	0.015414807307268111	0.0
42-43	2.546849617062059E-5	1.2734248085310294E-5	0.0	0.018732078933491443	0.0
44-45	2.546849617062059E-5	1.2734248085310294E-5	0.0	0.023551991833781385	0.0
46-47	2.546849617062059E-5	1.2734248085310294E-5	0.0	0.028352803361943367	0.0
48-49	2.546849617062059E-5	1.2734248085310294E-5	0.0	0.03320455188244659	0.0
50-51	2.546849617062059E-5	1.2734248085310294E-5	0.0	0.037884388053798124	0.0
52-53	2.546849617062059E-5	1.2734248085310294E-5	0.0	0.048956816763975425	0.0
54-55	2.546849617062059E-5	1.2734248085310294E-5	0.0	0.0626716019518546	0.0
56-57	2.546849617062059E-5	1.2734248085310294E-5	0.0	0.07741786123464392	0.0
58-59	2.546849617062059E-5	1.2734248085310294E-5	0.0	0.09115174779465107	0.0
60-61	2.546849617062059E-5	1.2734248085310294E-5	0.0	0.09785632941156695	0.0
62-63	2.546849617062059E-5	1.2734248085310294E-5	0.0	0.10564968923977686	0.0
64-65	2.546849617062059E-5	1.2734248085310294E-5	0.0	0.10987745960409986	0.0
66-67	2.546849617062059E-5	1.2734248085310294E-5	0.0	0.11320109835436586	0.0
68-69	2.546849617062059E-5	1.2734248085310294E-5	0.0	0.11646743298824794	0.0
70-71	2.546849617062059E-5	1.2734248085310294E-5	0.0	0.11946634841233852	0.0
72-73	2.546849617062059E-5	1.2734248085310294E-5	0.0	0.12187312130046217	0.0
74-75	2.546849617062059E-5	1.2734248085310294E-5	0.0	0.12398700648262367	0.0
76-77	2.546849617062059E-5	1.2734248085310294E-5	0.0	0.12611999303691313	0.0
78-79	2.546849617062059E-5	1.2734248085310294E-5	0.0	0.12760990006289447	0.0
80-81	2.546849617062059E-5	1.2734248085310294E-5	0.0	0.12810653573822156	0.0
82-83	2.546849617062059E-5	1.2734248085310294E-5	0.0	0.12856496866929273	0.0
84-85	2.546849617062059E-5	1.2734248085310294E-5	0.0	0.1287241467703591	0.0
86-87	2.546849617062059E-5	1.2734248085310294E-5	0.0	0.1288514892512122	0.0
88	2.546849617062059E-5	1.2734248085310294E-5	0.0	0.12885785637525485	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9615	0.0	46.65589	1
GTATCAA	16590	0.0	35.956257	1
ATCAACG	18420	0.0	31.332472	3
TCAACGC	18660	0.0	30.953075	4
CAACGCA	18990	0.0	30.36882	5
AACGCAG	19795	0.0	29.222776	6
TATCAAC	20300	0.0	28.821098	2
CGCAGAG	22330	0.0	25.96442	8
ACGCAGA	22345	0.0	25.907589	7
GCAGAGT	27200	0.0	21.153793	9
GAGTACT	17645	0.0	19.460897	12-13
TACATGG	18755	0.0	19.365046	2
GTGGTAT	4710	0.0	19.254309	1
GTACATG	20320	0.0	18.6102	1
AGAGTAC	24880	0.0	18.57043	10-11
ACATGGG	18970	0.0	18.565397	3
CAGAGTA	27495	0.0	18.237272	10-11
GTACTTT	19485	0.0	17.589897	14-15
GTGTAGC	6105	0.0	17.522747	1
TGGTATC	4820	0.0	16.988205	2
>>END_MODULE