FastQCFastQC Report
Fri 27 May 2016
SRR2139382_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139382_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18655884
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA269700.051.4482351
GTATCAA490650.037.8251721
TCAACGC598400.030.2886074
ATCAACG602800.030.0963123
CAACGCA614900.029.4896895
TATCAAC624250.029.348362
AACGCAG637150.028.458946
ACGCAGA735800.024.614267
CGCAGAG745050.024.3086688
GTACATG623650.021.307751
TACATGG617250.020.8464342
GCAGAGT863800.020.8094379
GAGTACT488800.019.93452812-13
ACATGGG634500.019.7567733
CAGAGTA839100.018.68473410-11
AGAGTAC780700.018.40867610-11
GTACTTT534400.018.12250114-15
CATGGGG436950.017.5518674
GTGGTAT123750.016.9804521
AGTACTT532000.016.97784612-13
Produced by FastQC (version 0.11.5)