##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139382_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18655884
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.429394822566437	31.0	31.0	34.0	28.0	34.0
2	31.797100421507768	33.0	31.0	34.0	30.0	34.0
3	31.912428164754886	33.0	31.0	34.0	30.0	34.0
4	35.47747091480629	37.0	35.0	37.0	33.0	37.0
5	35.55006854673839	37.0	35.0	37.0	33.0	37.0
6	35.5163050970943	37.0	35.0	37.0	33.0	37.0
7	35.52804943469846	37.0	35.0	37.0	33.0	37.0
8	35.63570351316507	37.0	35.0	37.0	33.0	37.0
9	37.44364973538644	39.0	37.0	39.0	34.0	39.0
10-11	37.50246201144904	39.0	37.0	39.0	34.5	39.0
12-13	37.46588175612584	39.0	37.0	39.0	34.5	39.0
14-15	38.75911811522842	40.0	38.0	41.0	34.0	41.0
16-17	38.773455924147044	40.0	38.0	41.0	34.5	41.0
18-19	38.826485386594385	40.0	38.0	41.0	35.0	41.0
20-21	38.84908295812477	40.0	38.0	41.0	34.5	41.0
22-23	38.80169592088434	40.0	38.0	41.0	34.0	41.0
24-25	38.7647590113674	40.0	38.0	41.0	34.0	41.0
26-27	38.676676103990175	40.0	38.0	41.0	34.0	41.0
28-29	38.60940175550661	40.0	38.0	41.0	34.0	41.0
30-31	38.525328095376814	40.0	38.0	41.0	34.0	41.0
32-33	38.4398935199321	40.0	38.0	41.0	34.0	41.0
34-35	38.37271832719925	40.0	38.0	41.0	34.0	41.0
36-37	38.318439057651744	40.0	38.0	41.0	34.0	41.0
38-39	38.27442907171152	40.0	38.0	41.0	34.0	41.0
40-41	38.220131484355804	40.0	38.0	41.0	33.0	41.0
42-43	38.15945487221714	40.0	38.0	41.0	33.0	41.0
44-45	38.078292337839386	40.0	37.5	41.0	33.0	41.0
46-47	37.992529722660244	40.0	37.0	41.0	33.0	41.0
48-49	37.91501474818321	40.0	37.0	41.0	33.0	41.0
50-51	37.82165670323231	40.0	37.0	41.0	33.0	41.0
52-53	37.70544499934154	40.0	37.0	41.0	32.5	41.0
54-55	37.567729909143736	39.5	36.0	41.0	32.0	41.0
56-57	37.38254413683728	39.0	36.0	41.0	32.0	41.0
58-59	37.21520035538419	39.0	35.5	41.0	31.5	41.0
60-61	37.002294887079124	39.0	35.0	40.5	31.0	41.0
62-63	36.760697174030284	38.0	35.0	40.0	31.0	41.0
64-65	36.500551140049154	38.0	35.0	40.0	31.0	41.0
66-67	36.21487499401112	37.0	35.0	40.0	31.0	41.0
68-69	35.928659845247005	37.0	34.0	39.5	30.0	41.0
70-71	35.60434753807463	36.0	34.0	39.0	30.0	41.0
72-73	35.269662863124	36.0	34.0	39.0	30.0	40.0
74-75	34.93560945159155	35.0	34.0	38.0	29.5	40.0
76-77	34.622307093817696	35.0	34.0	37.0	29.0	39.0
78-79	34.3102971501157	35.0	33.5	37.0	29.0	39.0
80-81	34.024764353418774	35.0	33.0	36.0	29.0	39.0
82-83	33.77237150984094	35.0	33.0	36.0	29.0	37.5
84-85	33.53767388305856	35.0	33.0	35.5	29.0	37.0
86-87	33.456321325722996	35.0	33.0	35.0	29.0	37.0
88-89	33.48631441710059	35.0	33.0	35.0	29.0	36.0
90-91	33.41127730527791	35.0	33.0	35.0	29.0	36.0
92-93	33.33480994488059	35.0	33.0	35.0	29.0	36.0
94-95	33.29972510619296	35.0	33.0	35.0	29.0	36.0
96-97	33.326654990045824	35.0	33.0	35.0	29.0	35.0
98-99	33.39029905098319	35.0	33.0	35.0	29.5	35.0
100	33.00784981808744	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	2.0
16	6.0
17	126.0
18	1241.0
19	4465.0
20	9466.0
21	16229.0
22	24976.0
23	38667.0
24	58877.0
25	87161.0
26	122976.0
27	165261.0
28	214186.0
29	269400.0
30	337121.0
31	420848.0
32	535348.0
33	702549.0
34	974598.0
35	1401032.0
36	2225730.0
37	3868115.0
38	4767878.0
39	2308915.0
40	100710.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.454616463095505	18.835703523885545	10.905213604458517	27.80446640856043
2	14.532283531759996	21.861531531970975	38.4000444257598	25.20614051050923
3	17.891708472449942	26.703623749183418	29.15531725125972	26.24935052710692
4	12.301421899921761	18.637583724255574	37.8820322853637	31.178962090458967
5	13.359264206075697	38.44087251826304	34.59832946487836	13.601533810782895
6	29.564912647082377	38.893770923227656	18.21571227928062	13.325604150409347
7	26.400919945685768	33.52134908214481	22.461422894782153	17.616308077387274
8	25.143638328797497	36.69775712584834	20.33296840825125	17.82563613710291
9	26.398700806673116	16.60726985652355	20.37778000763727	36.61624932916607
10-11	24.38512696584091	26.883679165243525	28.37371576710061	20.357478101814955
12-13	25.38909172033874	24.497008557729025	28.597977453118812	21.51592226881342
14-15	22.55064943585627	25.459876358579415	26.364095102649653	25.625379102914664
16-17	21.901181418152042	28.512631725197263	27.03631197535319	22.549874881297505
18-19	21.95957854369163	27.66641076884912	28.862869751977442	21.511140935481805
20-21	22.889338885887746	26.775893747092518	28.719590720947146	21.61517664607259
22-23	22.546717744775417	26.704989106477406	28.604611733836748	22.143681414910425
24-25	22.233697196039962	26.840384506745725	28.72958916465712	22.196329132557192
26-27	22.23907632227209	26.95911323156959	28.652483012297104	22.149327433861217
28-29	22.210351980674094	26.928798081600775	28.708768097205773	22.152081840519358
30-31	22.442993232374103	26.825599436903207	28.611448680182683	22.119958650540003
32-33	22.22102043575255	27.012365070098436	28.597972468144896	22.168642026004118
34-35	22.36759596662687	26.9921685391584	28.574263782394986	22.065971711819746
36-37	22.398036404487424	26.888148519579623	28.538255063803007	22.175560012129942
38-39	22.27488290101626	27.039618715864417	28.578627690629848	22.106870692489473
40-41	22.49944975619967	26.89457845498137	28.45139736639355	22.15457442242541
42-43	22.358875423583555	26.99675826538797	28.287126868894276	22.3572394421342
44-45	22.442733151079334	27.177438550001586	28.042108742901107	22.337719556017976
46-47	22.581686750304026	27.181070383591887	27.67183610660002	22.56540675950407
48-49	22.520215028760347	27.309487822928318	27.597891253285667	22.57240589502567
50-51	22.509359335460697	27.48357054158118	27.471405623419326	22.5356644995388
52-53	22.693427621654948	27.336379499330032	27.372999552582257	22.597193326432762
54-55	22.609680497509192	27.21428644037885	27.490797268859556	22.6852357932524
56-57	22.56917654093128	27.34926666382392	27.491995824390692	22.58956097085411
58-59	22.619149888079644	27.364136309372306	27.42958341867726	22.587130383870786
60-61	22.672904083310087	27.33427411715742	27.438164099152253	22.554657700380243
62-63	22.6009033095377	27.4760190962753	27.419111738601604	22.503965855585395
64-65	22.612447795507002	27.373934300437046	27.35651308865589	22.657104815400057
66-67	22.61864767387452	27.480892026702207	27.222620739930075	22.677839559493197
68-69	22.57865952907846	27.4356232615325	27.298956680268294	22.686760529120747
70-71	22.60272072611875	27.204340709395037	27.35207264179654	22.840865922689677
72-73	22.643353883752244	27.283146715892848	27.27500878776655	22.798490612588363
74-75	22.481324525486638	27.41751281690064	27.296936964311097	22.804225693301625
76-77	22.635710468309185	27.3230521704781	27.232119406142257	22.809117955070455
78-79	22.609210446034332	27.35276510341048	27.199139525862048	22.838884924693136
80-81	22.607258863766102	27.44374703937597	27.136049860138982	22.812944236718945
82-83	22.66865421711645	27.37328018280561	27.22104910109651	22.737016498981426
84-85	22.66402164507185	27.37539200177847	27.24258503928348	22.718001313866207
86-87	22.546614142167392	27.4097100074842	27.352859126807218	22.69081672354119
88-89	22.57714017929298	27.366819663888855	27.270666408900844	22.785373747917323
90-91	22.61796484680099	27.390681435302895	27.3141465317028	22.677207186193318
92-93	22.600230128914074	27.40038898752854	27.32060539836565	22.678775485191736
94-95	22.47121232194805	27.43136490103239	27.381526903025122	22.715895873994437
96-97	22.55572583338356	27.550196777891234	27.42966176679964	22.464415621925575
98-99	23.595678724621198	29.510191936301045	27.580952462384428	19.313176876693326
100	22.718825545133434	30.37458801519041	21.31324747125128	25.593338968424874
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8347.0
1	7365.0
2	4728.0
3	3139.5
4	3608.5
5	6418.0
6	10818.5
7	16683.5
8	23816.0
9	27309.5
10	25559.5
11	22636.5
12	24061.5
13	31973.5
14	52369.0
15	81291.0
16	104923.0
17	118466.0
18	120839.5
19	112696.0
20	97478.5
21	81009.0
22	70554.5
23	71437.5
24	82891.0
25	103300.5
26	131955.5
27	165338.0
28	199643.5
29	237498.5
30	274981.0
31	312626.5
32	355653.5
33	397306.5
34	439958.0
35	480613.5
36	522828.0
37	564130.5
38	597844.0
39	627340.0
40	655827.5
41	681742.0
42	702489.5
43	725711.5
44	749042.5
45	773405.5
46	788851.5
47	795468.5
48	801289.0
49	795068.0
50	783145.0
51	768392.5
52	741643.0
53	704657.5
54	663829.0
55	620380.0
56	575053.5
57	525182.5
58	471636.5
59	415231.5
60	352029.0
61	288175.0
62	230023.5
63	180210.5
64	137335.0
65	101955.0
66	75922.5
67	58394.5
68	45172.0
69	33814.5
70	24937.0
71	18503.5
72	13435.5
73	9337.0
74	6548.5
75	4585.0
76	3145.0
77	2089.0
78	1428.5
79	987.0
80	669.5
81	450.5
82	314.0
83	234.0
84	176.5
85	126.0
86	92.0
87	72.5
88	52.5
89	33.0
90	22.0
91	16.0
92	14.0
93	10.5
94	6.5
95	5.5
96	5.5
97	6.5
98	8.0
99	29.0
100	52.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	2.1976980560127842E-4
3	0.001988648728733519
4	0.0
5	0.09853727649678783
6	9.755635273032357E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	2.144629583465485E-5
22-23	1.555697803555869E-4
24-25	1.5297158576947742E-4
26-27	5.021830595822476E-4
28-29	1.6395169381048223E-4
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	3.666028110370344E-5
48-49	0.0
50-51	0.0
52-53	2.8923506984477388E-6
54-55	0.0
56-57	0.0
58-59	0.0
60-61	2.3867660030721257E-5
62-63	3.008415440511743E-4
64-65	0.001012991955448735
66-67	0.0
68-69	0.0
70-71	0.0
72-73	7.395200955145274E-4
74-75	1.6513732565944414E-4
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0010648423161987086
84-85	1.4370495434527021E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	4.2794267855145326E-4
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	2852.0
20-21	9340.0
22-23	10293.0
24-25	11390.0
26-27	14366.0
28-29	21065.0
30-31	38640.0
32-33	46953.0
34-35	62545.0
36-37	94615.0
38-39	120683.0
40-41	126807.0
42-43	145089.0
44-45	176646.0
46-47	171982.0
48-49	152796.0
50-51	133046.0
52-53	119564.0
54-55	126301.0
56-57	134688.0
58-59	140646.0
60-61	141817.0
62-63	134263.0
64-65	137341.0
66-67	146577.0
68-69	153700.0
70-71	156477.0
72-73	145491.0
74-75	142652.0
76-77	150244.0
78-79	160447.0
80-81	166569.0
82-83	159905.0
84-85	156322.0
86-87	161475.0
88-89	179252.0
90-91	197455.0
92-93	211223.0
94-95	253827.0
96-97	653305.0
98-99	1188613.0
100-101	1.1998622E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.45183893714183
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.49801070321786	70.39375864415594
2	8.193279312116578	13.18328775170838
3	2.210892889508405	5.336111959620068
4	0.8978128407775583	2.8892277624773546
5	0.43111261724147765	1.734190142304051
6	0.23734652658493638	1.145697871746081
7	0.15216938851453404	0.8569614995154256
8	0.08790400357689226	0.565763099015846
9	0.06583581605619046	0.4766951222683137
>10	0.21731533867224073	2.678425974103147
>50	0.005796515268645251	0.31307087218340424
>100	0.0023912317940940515	0.31769071641012103
>500	1.0331142861343587E-4	0.05907991390571548
>1k	2.9505241992337006E-5	0.050038670586065
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.36023916100679E-6
2	0.0	2.680119580503395E-5	0.0	0.0	5.36023916100679E-6
3	5.36023916100679E-6	2.680119580503395E-5	0.0	0.0	5.36023916100679E-6
4	5.36023916100679E-6	1.0184454405912902E-4	0.0	0.0	5.36023916100679E-6
5	5.36023916100679E-6	1.125650223811426E-4	0.0	0.0	5.36023916100679E-6
6	5.896263077107469E-5	1.125650223811426E-4	0.0	0.0	5.36023916100679E-6
7	5.896263077107469E-5	1.2328550070315618E-4	0.0	5.36023916100679E-6	5.36023916100679E-6
8	6.432286993208149E-5	1.554469356691969E-4	0.0	1.072047832201358E-5	5.36023916100679E-6
9	6.432286993208149E-5	3.216143496604074E-4	0.0	1.072047832201358E-5	2.144095664402716E-5
10-11	8.844394615661204E-5	3.242944692409108E-4	0.0	1.072047832201358E-5	2.144095664402716E-5
12-13	9.648430489812222E-5	3.269745888214142E-4	0.0	1.6080717483020372E-5	2.4121076224530555E-5
14-15	9.648430489812222E-5	4.5294020910507373E-4	0.0	2.144095664402716E-5	3.4841554546544136E-5
16-17	1.527668160886935E-4	4.6098056784658393E-4	0.0	2.4121076224530555E-5	3.752167412704753E-5
18-19	1.608071748302037E-4	4.824215244906111E-4	0.0	3.2161434966040744E-5	4.556203286855771E-5
20-21	1.608071748302037E-4	5.226233181981621E-4	0.0	3.2161434966040744E-5	5.092227202956451E-5
22-23	1.7152765315221727E-4	6.217877426767876E-4	0.0	3.752167412704753E-5	6.164275035157809E-5
24-25	1.9832884895725123E-4	6.753901342868556E-4	0.0	6.700298951258487E-5	7.504334825409506E-5
26-27	2.090493272792648E-4	7.021913300918895E-4	0.0	1.4204633776667994E-4	7.504334825409506E-5
28-29	2.090493272792648E-4	7.048714496723929E-4	0.0	6.459088189013182E-4	7.772346783459845E-5
30-31	2.090493272792648E-4	7.825949175069913E-4	0.0	0.0022620209259448655	8.040358741510185E-5
32-33	2.117294468597682E-4	8.013557545705151E-4	0.0	0.005513005977095484	8.040358741510185E-5
34-35	2.2781016434278857E-4	8.897997007271272E-4	0.0	0.009699352761841785	8.576382657610864E-5
36-37	2.3585052308429876E-4	9.728834077227324E-4	0.0	0.016825790726400315	8.576382657610864E-5
38-39	2.3585052308429876E-4	0.0010157653210107868	0.0	0.034262648717155404	9.112406573711543E-5
40-41	2.3585052308429876E-4	0.0011470911804554531	0.0	0.05339602240236914	9.648430489812222E-5
42-43	2.3585052308429876E-4	0.001192653213324011	0.0	0.06677249922866159	9.916442447862562E-5
44-45	2.3853064266480216E-4	0.001337379670671194	0.0	0.08221534825152216	1.0452466363963241E-4
46-47	2.4121076224530556E-4	0.0014338639755693164	0.0	0.09752419129535754	1.098849028006392E-4
48-49	2.4121076224530556E-4	0.0014392242147303232	0.0	0.11261326453359166	1.125650223811426E-4
50-51	2.4121076224530556E-4	0.0016241524657850575	0.0	0.1275710119123811	1.1524514196164598E-4
52-53	2.4121076224530556E-4	0.001634872944107071	0.0	0.15719705375526563	1.2864573986416298E-4
54-55	2.4121076224530556E-4	0.0016509536615900914	0.0	0.190628865402465	1.3936621818617654E-4
56-57	2.4121076224530556E-4	0.0016991958140391524	0.0	0.22306099244613656	1.4204633776667994E-4
58-59	2.4121076224530556E-4	0.0017018759336196558	0.0	0.24531938556221727	1.4472645734718334E-4
60-61	2.5461136014782255E-4	0.001723316890263683	0.0	0.25673133473600074	1.500866965081901E-4
62-63	2.840926755333599E-4	0.0017447578469077102	0.0	0.2694243810692648	1.554469356691969E-4
64-65	2.840926755333599E-4	0.0018010403580982816	0.0	0.27580842591002386	1.554469356691969E-4
66-67	2.974932734358768E-4	0.0018090807168397918	0.0	0.28048791469758283	1.608071748302037E-4
68-69	3.001733930163802E-4	0.0018358819126448257	0.0	0.2852263661159128	1.715276531522173E-4
70-71	3.082137517578904E-4	0.0018573228692888527	0.0	0.28960300139087486	1.8224813147423087E-4
72-73	3.269745888214142E-4	0.0018760837063523765	0.0	0.2928861478769915	2.090493272792648E-4
74-75	3.269745888214142E-4	0.0019350463371234513	0.0	0.2955099849463043	2.090493272792648E-4
76-77	3.376950671434278E-4	0.002082452914051138	0.0	0.2979917756778505	2.090493272792648E-4
78-79	3.4841554546544135E-4	0.002165536621046743	0.0	0.2995006830016739	2.090493272792648E-4
80-81	3.4841554546544135E-4	0.002165536621046743	0.0	0.29999918524364755	2.17089686020775E-4
82-83	3.5109566504594475E-4	0.0021789372189492603	0.0	0.3004816067681381	2.2781016434278857E-4
84-85	3.5377578462644814E-4	0.0022030582951737907	0.0	0.30066921513877337	2.3049028392329197E-4
86-87	3.5645590420695154E-4	0.002224499251817818	0.0	0.30074961872618844	2.3049028392329197E-4
88	3.5913602378745494E-4	0.002390666665809028	0.0	0.30075765908492996	2.3585052308429876E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	26970	0.0	51.448235	1
GTATCAA	49065	0.0	37.825172	1
TCAACGC	59840	0.0	30.288607	4
ATCAACG	60280	0.0	30.096312	3
CAACGCA	61490	0.0	29.489689	5
TATCAAC	62425	0.0	29.34836	2
AACGCAG	63715	0.0	28.45894	6
ACGCAGA	73580	0.0	24.61426	7
CGCAGAG	74505	0.0	24.308668	8
GTACATG	62365	0.0	21.30775	1
TACATGG	61725	0.0	20.846434	2
GCAGAGT	86380	0.0	20.809437	9
GAGTACT	48880	0.0	19.934528	12-13
ACATGGG	63450	0.0	19.756773	3
CAGAGTA	83910	0.0	18.684734	10-11
AGAGTAC	78070	0.0	18.408676	10-11
GTACTTT	53440	0.0	18.122501	14-15
CATGGGG	43695	0.0	17.551867	4
GTGGTAT	12375	0.0	16.980452	1
AGTACTT	53200	0.0	16.977846	12-13
>>END_MODULE