FastQCFastQC Report
Fri 27 May 2016
SRR2139381_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139381_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19399476
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA268600.051.7947651
GTATCAA490200.037.2410961
TCAACGC582800.030.6330834
ATCAACG586650.030.4472643
CAACGCA597000.029.9402985
TATCAAC612900.029.3247662
AACGCAG618650.028.8858456
ACGCAGA717200.024.8388467
CGCAGAG730300.024.3932918
GTACATG631850.020.8666441
GCAGAGT854950.020.741799
TACATGG629550.020.4330732
GAGTACT481250.019.75660312-13
ACATGGG639800.019.7311633
CAGAGTA826750.018.7526110-11
AGAGTAC768150.018.3493710-11
GTACTTT524850.018.07453214-15
CATGGGG458850.017.5328714
AGTACTT525950.016.80135212-13
GTGGTAT120300.016.1141531
Produced by FastQC (version 0.11.5)