FastQCFastQC Report
Fri 27 May 2016
SRR2139380_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139380_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18646662
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA289150.052.847971
GTATCAA533550.037.7396661
TCAACGC662650.029.8427724
ATCAACG663550.029.777483
CAACGCA675150.029.2965285
TATCAAC682700.029.1905842
AACGCAG694750.028.5386336
ACGCAGA803300.024.6028527
CGCAGAG815200.024.24898
GTACATG714200.021.6391581
TACATGG709350.021.4108522
GCAGAGT941700.020.8207349
ACATGGG724550.020.4236453
GAGTACT522650.019.62763412-13
CAGAGTA914100.018.6854610-11
AGAGTAC861100.018.37096610-11
GTACTTT574150.017.87165514-15
CATGGGG461150.017.3808694
AGTACTT570250.017.24714312-13
GTGGTAT125600.017.1348151
Produced by FastQC (version 0.11.5)