##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139380_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18646662
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.45879680770746	31.0	31.0	34.0	28.0	34.0
2	31.82456645591581	33.0	31.0	34.0	30.0	34.0
3	31.933387916829297	33.0	31.0	34.0	30.0	34.0
4	35.489542417833285	37.0	35.0	37.0	33.0	37.0
5	35.56093305064467	37.0	35.0	37.0	33.0	37.0
6	35.52896668583364	37.0	35.0	37.0	33.0	37.0
7	35.541155837972504	37.0	35.0	37.0	33.0	37.0
8	35.650122633209094	37.0	35.0	37.0	33.0	37.0
9	37.46053728007726	39.0	37.0	39.0	34.0	39.0
10-11	37.52133998567679	39.0	37.0	39.0	35.0	39.0
12-13	37.48396501207562	39.0	37.0	39.0	34.5	39.0
14-15	38.77662401452871	40.0	38.0	41.0	34.5	41.0
16-17	38.791467609591464	40.0	38.0	41.0	35.0	41.0
18-19	38.84870348376562	40.0	38.5	41.0	35.0	41.0
20-21	38.87331579179322	40.0	38.0	41.0	35.0	41.0
22-23	38.82399790964658	40.0	38.0	41.0	34.5	41.0
24-25	38.78625937438869	40.0	38.0	41.0	34.0	41.0
26-27	38.697546329204634	40.0	38.0	41.0	34.0	41.0
28-29	38.63315866713934	40.0	38.0	41.0	34.0	41.0
30-31	38.54593518925381	40.0	38.0	41.0	34.0	41.0
32-33	38.458233154899546	40.0	38.0	41.0	34.0	41.0
34-35	38.39462257413737	40.0	38.0	41.0	34.0	41.0
36-37	38.34231470068892	40.0	38.0	41.0	34.0	41.0
38-39	38.303464182221944	40.0	38.0	41.0	34.0	41.0
40-41	38.25120238091768	40.0	38.0	41.0	33.5	41.0
42-43	38.19398089484212	40.0	38.0	41.0	33.0	41.0
44-45	38.116891968067414	40.0	38.0	41.0	33.0	41.0
46-47	38.03499854342664	40.0	37.0	41.0	33.0	41.0
48-49	37.962304497687285	40.0	37.0	41.0	33.0	41.0
50-51	37.8728862562981	40.0	37.0	41.0	33.0	41.0
52-53	37.762739037485645	40.0	37.0	41.0	33.0	41.0
54-55	37.63484814175925	39.5	36.5	41.0	32.5	41.0
56-57	37.455227767480224	39.0	36.0	41.0	32.0	41.0
58-59	37.290624108067306	39.0	36.0	41.0	32.0	41.0
60-61	37.08177366127609	39.0	35.0	41.0	31.0	41.0
62-63	36.84826584097969	38.5	35.0	40.0	31.0	41.0
64-65	36.58922664762088	38.0	35.0	40.0	31.0	41.0
66-67	36.305768237317366	37.5	35.0	40.0	31.0	41.0
68-69	36.02261451119375	37.0	34.5	39.5	31.0	41.0
70-71	35.6971520603694	36.5	34.0	39.0	30.0	41.0
72-73	35.361884733165915	36.0	34.0	39.0	30.0	40.5
74-75	35.02356586927285	35.5	34.0	38.5	30.0	40.0
76-77	34.70718683633679	35.0	34.0	37.0	29.5	39.0
78-79	34.39227164611758	35.0	34.0	37.0	29.0	39.0
80-81	34.10337075200572	35.0	33.0	36.5	29.0	39.0
82-83	33.845139427562984	35.0	33.0	36.0	29.0	37.5
84-85	33.60531304244177	35.0	33.0	36.0	29.0	37.0
86-87	33.51938781224696	35.0	33.0	35.0	29.0	37.0
88-89	33.54321124148089	35.0	33.0	35.0	29.0	36.0
90-91	33.46165945226677	35.0	33.0	35.0	29.0	36.0
92-93	33.38039123186251	35.0	33.0	35.0	29.0	36.0
94-95	33.34079985849537	35.0	33.0	35.0	29.0	36.0
96-97	33.364264778479395	35.0	33.0	35.0	29.0	35.0
98-99	33.42466171036121	35.0	33.0	35.0	30.0	35.0
100	33.04088057538411	34.0	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	2.0
17	119.0
18	1250.0
19	4577.0
20	9737.0
21	16111.0
22	24842.0
23	37684.0
24	56477.0
25	82693.0
26	117219.0
27	158134.0
28	205491.0
29	261030.0
30	326412.0
31	412423.0
32	525969.0
33	692660.0
34	963008.0
35	1387189.0
36	2196091.0
37	3845792.0
38	4807820.0
39	2403731.0
40	110200.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.64154088275961	18.91613630364512	10.93307745911842	27.509245354476853
2	14.551330434291089	21.922350744783852	38.61732460009638	24.908994220828678
3	17.923788663272852	26.900061197189938	29.07308969547332	26.103060444063885
4	12.13197836695919	18.81719634323827	38.07576927173346	30.97505601806908
5	13.185327997748752	38.643760293370896	34.56451072523684	13.606400983643502
6	29.215451346069145	39.058864678956276	18.410494549668062	13.315189425306514
7	26.319321924749854	33.647169665004924	22.487333121606433	17.546175288638793
8	25.134691667602493	36.674617687605426	20.375888188459683	17.814802456332398
9	26.485979099100952	16.514880786705955	20.444104151188025	36.55503596300507
10-11	24.32809690013151	26.9976015010086	28.372820829808575	20.30148076905132
12-13	25.410400531741285	24.678239998129424	28.56246066990435	21.34889880022494
14-15	22.540777003412195	25.529976893451494	26.538296773974878	25.390949329161433
16-17	21.69953796556188	28.577693959379967	27.241873103078717	22.480894971979435
18-19	21.74018599146593	27.716051805947895	29.14814458480558	21.395617617780598
20-21	22.80779715492742	26.76727011066651	29.003669393266975	21.4212633411391
22-23	22.451508897868102	26.716667039237684	28.854933967847273	21.976890095046937
24-25	22.08727886475022	26.852571488102257	29.021440187994664	22.03870945915286
26-27	22.022563920575912	27.035961547328093	28.904525304759467	22.03694922733653
28-29	22.031895199106874	27.003909198345966	28.998358143148533	21.965837459398625
30-31	22.30325061796184	26.90367658682017	28.849539386500357	21.94353340871763
32-33	22.051469343155567	27.041585053284695	28.882859600116873	22.024086003442864
34-35	22.21072222556168	27.0416473302261	28.877157400741776	21.870473043470447
36-37	22.213961601999895	26.94263188087883	28.83269910137023	22.010707415751046
38-39	22.07931207806935	27.088322621540804	28.89399798074837	21.93836731964148
40-41	22.330751627808024	26.929082860986643	28.73049639376475	22.009669117440588
42-43	22.193015923245845	27.058702423313445	28.493154880011023	22.25512677342968
44-45	22.287389060619418	27.275045681950605	28.245235301307154	22.19232995612282
46-47	22.459053909377808	27.266161071021898	27.851754665496102	22.42303035410419
48-49	22.436172167654057	27.370022756673517	27.741380999955744	22.45242407571668
50-51	22.410695886880184	27.525829552145826	27.583887751877747	22.479586809096244
52-53	22.533791286708954	27.464043681700634	27.511776157892776	22.49038887369764
54-55	22.470336263252975	27.354845549771362	27.584318412732795	22.59049977424287
56-57	22.507376492863056	27.469238662737894	27.54578081862496	22.477604025774088
58-59	22.49061730813475	27.48915139988055	27.54260885303697	22.47762243894773
60-61	22.585632839073796	27.49235410476848	27.525598630703136	22.39641442545459
62-63	22.524636144496462	27.588081116393724	27.45641134202842	22.430871397081393
64-65	22.512354785605673	27.470951769200898	27.456155966719294	22.560537478474142
66-67	22.54469353848341	27.533238497821284	27.357862109099667	22.564205854595638
68-69	22.480607821087666	27.524215236527848	27.416628981015272	22.578547961369207
70-71	22.5259309146638	27.28294275310089	27.50419297502088	22.68693335721443
72-73	22.561741144216196	27.357698901064285	27.476406466491582	22.604153488227936
74-75	22.40054146308302	27.525312492077305	27.42771605983107	22.646429985008613
76-77	22.52247827828506	27.414982710659213	27.390333336600936	22.672205674454787
78-79	22.528128653489663	27.452212865839158	27.377003776957697	22.64265470371348
80-81	22.527858662326377	27.526679568922525	27.338648937664757	22.60681283108634
82-83	22.545136118460817	27.46176118973857	27.4427125947453	22.550390097055313
84-85	22.574973961114996	27.497859295088595	27.363428023093277	22.56373872070313
86-87	22.450779442570497	27.55977931731211	27.485586499445752	22.503854740671635
88-89	22.524237171337145	27.487954365103263	27.391074359606115	22.596734103953477
90-91	22.53026139269961	27.493811808288736	27.426767602704544	22.549159196307105
92-93	22.53845549766527	27.529901361978652	27.417161558559798	22.514481581796275
94-95	22.393488849963784	27.549143086949464	27.488601011773518	22.568767051313245
96-97	22.451966268939607	27.692231016531366	27.532337124488343	22.323465590040684
98-99	23.450004773718543	29.666381842789697	27.728515987639184	19.155097395852575
100	22.55428894601352	30.546492216355208	21.390181738288014	25.509037099343256
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8337.0
1	7389.0
2	4894.0
3	3403.5
4	3942.0
5	7310.0
6	12351.5
7	19021.5
8	27093.5
9	30787.5
10	28488.5
11	24913.5
12	26675.0
13	35834.0
14	59427.0
15	92636.5
16	119165.0
17	133566.5
18	134328.0
19	122884.0
20	104551.0
21	85633.0
22	73365.5
23	73454.5
24	84649.5
25	105275.0
26	134060.0
27	167514.0
28	201508.0
29	238928.0
30	278160.5
31	317709.0
32	362472.0
33	408985.5
34	454459.0
35	495570.0
36	538032.0
37	579957.0
38	612752.0
39	638693.0
40	664618.0
41	688348.5
42	707913.0
43	731140.0
44	755092.0
45	778276.0
46	790887.5
47	798787.5
48	805791.5
49	798692.0
50	788144.5
51	773377.5
52	742303.5
53	703463.5
54	663193.5
55	615049.5
56	565817.0
57	514996.0
58	458309.0
59	400327.0
60	338812.5
61	277297.5
62	221488.0
63	172210.0
64	129093.0
65	95284.5
66	69876.5
67	51634.0
68	38803.5
69	27940.5
70	19735.0
71	14016.5
72	9883.0
73	6749.5
74	4502.5
75	3000.0
76	2106.0
77	1436.0
78	995.0
79	709.0
80	488.5
81	338.5
82	247.5
83	174.5
84	130.5
85	101.0
86	70.5
87	52.0
88	35.0
89	25.0
90	21.5
91	15.5
92	11.0
93	8.5
94	5.5
95	3.5
96	4.0
97	5.5
98	8.5
99	28.5
100	50.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	2.3596716667036705E-4
3	0.002043261147759315
4	0.0
5	0.10068826259627595
6	9.81408897742663E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	1.341073904624916E-5
22-23	1.7711628126655852E-4
24-25	1.557311701951591E-4
26-27	5.293034052828618E-4
28-29	1.7748028019295872E-4
30-31	2.692937456446786E-6
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	2.546833581234568E-5
48-49	0.0
50-51	0.0
52-53	2.9080342880506837E-6
54-55	0.0
56-57	0.0
58-59	0.0
60-61	1.2025509834266528E-5
62-63	2.729591225516704E-4
64-65	8.501856386458426E-4
66-67	0.0
68-69	0.0
70-71	0.0
72-73	6.033465665229919E-4
74-75	9.935915906510338E-5
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0011022211225247035
84-85	7.427136582948658E-5
86-87	0.0
88-89	0.0
90-91	0.0
92-93	3.5099221244005746E-4
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	3123.0
20-21	9265.0
22-23	9958.0
24-25	11623.0
26-27	14277.0
28-29	21773.0
30-31	39692.0
32-33	47846.0
34-35	64595.0
36-37	98229.0
38-39	131070.0
40-41	134405.0
42-43	153886.0
44-45	189979.0
46-47	184581.0
48-49	163741.0
50-51	142464.0
52-53	130075.0
54-55	137220.0
56-57	143034.0
58-59	146470.0
60-61	148670.0
62-63	137329.0
64-65	139143.0
66-67	148064.0
68-69	154842.0
70-71	158572.0
72-73	147266.0
74-75	143666.0
76-77	151717.0
78-79	161793.0
80-81	167773.0
82-83	161164.0
84-85	156398.0
86-87	161794.0
88-89	178968.0
90-91	197994.0
92-93	209883.0
94-95	250361.0
96-97	638930.0
98-99	1171072.0
100-101	1.1883957E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.95529634504754
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.29559709839383	69.79745335619951
2	8.176760118475945	13.075505568302207
3	2.299891237893538	5.516654564614682
4	0.968117497737786	3.0962448571380228
5	0.46157000478425625	1.845248325825511
6	0.24894650108048955	1.1942754760771943
7	0.14700622999094026	0.8227748678445692
8	0.10051047941257567	0.642907613377223
9	0.067365319282332	0.484759265993784
>10	0.22492228749879362	2.756822623253521
>50	0.006678553455741767	0.3611066824497302
>100	0.0025317727116313314	0.3339089760610957
>500	8.820277721581137E-5	0.05090466619473352
>1k	1.469650501653954E-5	0.02143315666833028
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.3628901515992516E-6
2	0.0	5.3628901515992516E-5	0.0	0.0	5.3628901515992516E-6
3	0.0	5.3628901515992516E-5	0.0	5.3628901515992516E-6	5.3628901515992516E-6
4	0.0	8.044335227398878E-5	0.0	2.1451560606397006E-5	1.0725780303198503E-5
5	1.6088670454797755E-5	8.044335227398878E-5	0.0	2.1451560606397006E-5	1.0725780303198503E-5
6	1.662495946995768E-4	8.044335227398878E-5	0.0	3.754023106119476E-5	2.1451560606397006E-5
7	1.662495946995768E-4	8.044335227398878E-5	0.0	4.290312121279401E-5	2.6814450757996258E-5
8	1.8233826515437455E-4	9.116913257718727E-5	0.0	4.290312121279401E-5	3.754023106119476E-5
9	1.8233826515437455E-4	2.1451560606397006E-4	0.0	5.8991791667591764E-5	4.826601136439326E-5
10-11	2.091527159123708E-4	2.1451560606397006E-4	0.0	7.508046212238953E-5	4.826601136439326E-5
12-13	2.4133005682196632E-4	2.198784962155693E-4	0.0	8.580624242558802E-5	5.362890151599251E-5
14-15	2.708259526557622E-4	2.7887028788316107E-4	0.0	8.580624242558802E-5	8.580624242558802E-5
16-17	3.9149098106674535E-4	2.8423317803476033E-4	0.0	1.0725780303198503E-4	1.0457635795618541E-4
18-19	4.0221676136994387E-4	3.056847386411573E-4	0.0	1.4211658901738014E-4	1.367536988657809E-4
20-21	4.1562398674894194E-4	3.2981774432335397E-4	0.0	1.6356814962377716E-4	1.6088670454797755E-4
22-23	4.478013276585375E-4	3.6735797538454875E-4	0.0	1.7965682007857492E-4	1.7965682007857492E-4
24-25	4.907044488713315E-4	3.9149098106674535E-4	0.0	2.091527159123708E-4	1.9574549053337267E-4
26-27	5.148374545535281E-4	4.0221676136994387E-4	0.0	2.949589583379588E-4	1.984269356091723E-4
28-29	5.282446799325263E-4	4.1294254167314234E-4	0.0	5.282446799325263E-4	2.0647127083657117E-4
30-31	5.416519053115243E-4	4.880230037955319E-4	0.0	0.0012575977405500244	2.091527159123708E-4
32-33	5.523776856147229E-4	4.907044488713315E-4	0.0	0.0026680378504206274	2.1451560606397006E-4
34-35	6.006436969791161E-4	5.550591306905225E-4	0.0	0.004518234952722369	2.198784962155693E-4
36-37	6.381839280403109E-4	6.274581477371125E-4	0.0	0.007036111878898218	2.198784962155693E-4
38-39	6.381839280403109E-4	6.462282632677098E-4	0.0	0.011109226949037848	2.3864861174616669E-4
40-41	6.381839280403109E-4	7.23990170465899E-4	0.0	0.015820525947217792	2.4669294697356555E-4
42-43	6.435468181919102E-4	7.454417310722959E-4	0.0	0.019751524428340043	2.520558371251648E-4
44-45	6.542725984951087E-4	8.553809791800805E-4	0.0	0.024092784006059638	2.547372822009644E-4
46-47	6.596354886467079E-4	9.38505776529869E-4	0.0	0.02880140155916378	2.6278161742836334E-4
48-49	6.596354886467079E-4	9.411872216056686E-4	0.0	0.03322310448915736	2.7887028788316107E-4
50-51	6.864499394047042E-4	0.0010752594753956498	0.0	0.037883456030897114	2.8691462311056E-4
52-53	6.971757197079027E-4	0.0010833038106230488	0.0	0.04263229526013825	3.1641051894435585E-4
54-55	6.971757197079027E-4	0.0010940295909262474	0.0	0.04677512790224867	3.432249697023521E-4
56-57	6.971757197079027E-4	0.001110118261381045	0.0	0.050617638695869535	3.566321950813502E-4
58-59	6.971757197079027E-4	0.001110118261381045	0.0	0.054433335038732406	3.807652007635469E-4
60-61	7.347159507690974E-4	0.001123525486760043	0.0	0.05791116930204451	3.9149098106674535E-4
62-63	7.990706325882884E-4	0.001153021382593839	0.0	0.06119593951989906	4.183054318247416E-4
64-65	7.990706325882884E-4	0.0011851987235034346	0.0	0.06408117442145946	4.183054318247416E-4
66-67	8.124778579672867E-4	0.0011851987235034346	0.0	0.06687255874536686	4.2098687690054126E-4
68-69	8.31247973497884E-4	0.00121737606441303	0.0	0.06957277393669709	4.317126572037398E-4
70-71	8.580624242558801E-4	0.0012388276250194271	0.0	0.071661619650745	4.5852710796173604E-4
72-73	9.009655454686742E-4	0.0012441905151710263	0.0	0.07357080854471433	4.719343333407341E-4
74-75	9.009655454686742E-4	0.0012790493011564214	0.0	0.07504024044625253	4.7729722349233337E-4
76-77	9.143727708476724E-4	0.0013836256591126068	0.0	0.07640509598983454	4.987487840987304E-4
78-79	9.277799962266705E-4	0.0014426174507801985	0.0	0.07741600078341099	5.0143022917453E-4
80-81	9.277799962266705E-4	0.0014426174507801985	0.0	0.07769487107129416	5.309261250083259E-4
82-83	9.358243314540693E-4	0.0014452988958559981	0.0	0.07801664448039011	5.631034659179213E-4
84-85	9.465501117572679E-4	0.001447980340931798	0.0	0.07818557552016547	5.684663560695205E-4
86-87	9.492315568330675E-4	0.0014587061212349964	0.0	0.07824724875690887	5.818735814485188E-4
88	9.706831174394645E-4	0.0015981412651765768	0.0	0.07824993020198467	5.952808068275169E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	28915	0.0	52.84797	1
GTATCAA	53355	0.0	37.739666	1
TCAACGC	66265	0.0	29.842772	4
ATCAACG	66355	0.0	29.77748	3
CAACGCA	67515	0.0	29.296528	5
TATCAAC	68270	0.0	29.190584	2
AACGCAG	69475	0.0	28.538633	6
ACGCAGA	80330	0.0	24.602852	7
CGCAGAG	81520	0.0	24.2489	8
GTACATG	71420	0.0	21.639158	1
TACATGG	70935	0.0	21.410852	2
GCAGAGT	94170	0.0	20.820734	9
ACATGGG	72455	0.0	20.423645	3
GAGTACT	52265	0.0	19.627634	12-13
CAGAGTA	91410	0.0	18.68546	10-11
AGAGTAC	86110	0.0	18.370966	10-11
GTACTTT	57415	0.0	17.871655	14-15
CATGGGG	46115	0.0	17.380869	4
AGTACTT	57025	0.0	17.247143	12-13
GTGGTAT	12560	0.0	17.134815	1
>>END_MODULE