FastQCFastQC Report
Fri 27 May 2016
SRR2139379_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139379_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5395051
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58700.049.795581
ATCTCGT39250.040.39109894
GTATCAA112100.039.5051041
TATCTCG49450.036.08284494
TCAACGC130500.033.191694
ATCAACG131650.032.901753
CAACGCA133400.032.4371345
TATGCCG21450.031.83714386-87
AACGCAG138200.031.3090656
TATCAAC142350.030.8317762
ATGCCGT23650.030.6222388-89
TGCCGTC22750.030.5462788-89
GCCGTCT23950.030.33711290-91
TCTCGTA23550.028.9126482-83
CTCGTAT23900.028.38090982-83
CCGTCTT25000.028.19862790-91
TCGTATG24900.028.12140784-85
ACGCAGA157400.027.4899167
CGTATGC25150.027.42632184-85
CGCAGAG160200.027.0094418
Produced by FastQC (version 0.11.5)