##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139379_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5395051
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.971473856317576	32.0	25.0	33.0	18.0	33.0
2	30.036761468983332	32.0	29.0	33.0	25.0	33.0
3	31.255233175738283	33.0	31.0	33.0	28.0	33.0
4	31.795833255329747	33.0	31.0	33.0	29.0	33.0
5	32.62683726252078	33.0	33.0	33.0	32.0	34.0
6	36.54343582664927	38.0	37.0	38.0	34.0	38.0
7	36.772691676130584	38.0	37.0	38.0	34.0	38.0
8	36.94823422429186	38.0	38.0	38.0	35.0	38.0
9	37.16680379851831	38.0	38.0	38.0	36.0	38.0
10-11	37.2648565324035	38.0	38.0	38.0	36.5	38.0
12-13	37.31835046601043	38.0	38.0	38.0	37.0	38.0
14-15	37.32237711932658	38.0	38.0	38.0	37.0	38.0
16-17	37.32195043197923	38.0	38.0	38.0	37.0	38.0
18-19	37.32767660583746	38.0	38.0	38.0	37.0	38.0
20-21	37.2943691330812	38.0	38.0	38.0	37.0	38.0
22-23	37.309613565306094	38.0	38.0	38.0	37.0	38.0
24-25	37.3331329710645	38.0	38.0	38.0	37.0	38.0
26-27	37.31061893982434	38.0	38.0	38.0	37.0	38.0
28-29	37.29079568124811	38.0	38.0	38.0	37.0	38.0
30-31	37.290390204480516	38.0	38.0	38.0	37.0	38.0
32-33	37.2289192215537	38.0	38.0	38.0	37.0	38.0
34-35	37.243090144264826	38.0	38.0	38.0	37.0	38.0
36-37	37.243615262387706	38.0	38.0	38.0	37.0	38.0
38-39	37.23536019208281	38.0	38.0	38.0	37.0	38.0
40-41	37.224989563066735	38.0	38.0	38.0	37.0	38.0
42-43	37.196692541987105	38.0	38.0	38.0	36.0	38.0
44-45	37.172427449945154	38.0	38.0	38.0	36.0	38.0
46-47	37.14732750214803	38.0	38.0	38.0	36.0	38.0
48-49	37.10949930528001	38.0	38.0	38.0	36.0	38.0
50-51	37.08009626645541	38.0	38.0	38.0	36.0	38.0
52-53	37.046306329214204	38.0	38.0	38.0	36.0	38.0
54-55	37.02074075035811	38.0	38.0	38.0	36.0	38.0
56-57	36.990472950137544	38.0	38.0	38.0	36.0	38.0
58-59	36.99385079549946	38.0	38.0	38.0	36.0	38.0
60-61	37.01698401613739	38.0	38.0	38.0	36.0	38.0
62-63	37.02221342754427	38.0	38.0	38.0	36.0	38.0
64-65	37.01904369446683	38.0	38.0	38.0	36.0	38.0
66-67	37.02375057334611	38.0	38.0	38.0	36.0	38.0
68-69	37.02312933258885	38.0	38.0	38.0	36.0	38.0
70-71	37.01030730753948	38.0	38.0	38.0	36.0	38.0
72-73	36.98849665161977	38.0	38.0	38.0	36.0	38.0
74-75	36.97641997798912	38.0	38.0	38.0	36.0	38.0
76-77	36.97082136223706	38.0	38.0	38.0	36.0	38.0
78-79	36.93996351593987	38.0	38.0	38.0	36.0	38.0
80-81	36.91352237427073	38.0	38.0	38.0	35.0	38.0
82-83	36.897776991101225	38.0	38.0	38.0	35.0	38.0
84-85	36.85800795488586	38.0	38.0	38.0	35.0	38.0
86-87	36.84528637252678	38.0	38.0	38.0	35.0	38.0
88-89	36.851881637045544	38.0	38.0	38.0	35.0	38.0
90-91	36.83250011217369	38.0	38.0	38.0	35.0	38.0
92-93	36.8346922162912	38.0	38.0	38.0	35.0	38.0
94-95	36.762573506325054	38.0	38.0	38.0	35.0	38.0
96-97	36.69773483302546	38.0	38.0	38.0	34.0	38.0
98-99	36.78308058470385	38.0	38.0	38.0	35.0	38.0
100	35.11784470362091	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	13.0
19	28.0
20	65.0
21	155.0
22	454.0
23	1256.0
24	2995.0
25	5608.0
26	9724.0
27	15674.0
28	23794.0
29	34315.0
30	47449.0
31	62792.0
32	83762.0
33	113791.0
34	167123.0
35	292670.0
36	804079.0
37	3729302.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.076006695766175	19.500557084631822	14.27156110294416	26.15187511665784
2	13.468993505350053	20.62536075724823	43.228236583352206	22.677409154049506
3	17.227622129985427	26.82020985529145	30.846307106272025	25.1058609084511
4	11.945596065727646	18.817560760778722	39.57890296125097	29.657940212242668
5	13.189803655226259	37.6106529329376	36.07734194212348	13.122201469712664
6	29.084678444042883	38.65208980186034	19.355748148343118	12.907483605753663
7	25.92913394145857	33.00675007520781	23.672899477687977	17.391216505645637
8	23.999013169662344	36.79579674038299	21.652714682400592	17.552475407554073
9	25.40465326463086	17.06821677867364	21.550991825656514	35.97613813103898
10-11	23.634484641572435	26.909069070894788	29.556856830454432	19.89958945707835
12-13	24.70124934870866	24.47746091742228	29.448470459315402	21.372819274553663
14-15	21.877003572348062	25.850478521889787	27.034480304264036	25.238037601498114
16-17	21.42679466792807	29.048817147418998	27.66246324640861	21.86192493824433
18-19	21.49706277104702	28.227240113207458	29.323485542583377	20.952211573162145
20-21	22.315679351961368	27.446822392713827	29.14221109337242	21.095287161952385
22-23	22.003641451000984	27.3185194521975	29.016553355969215	21.661285740832305
24-25	21.802281798066442	27.41048554178129	29.081353507602053	21.70587915255021
26-27	21.749876022524507	27.61462702477232	28.959080481078953	21.676416471624222
28-29	21.703300116393105	27.63674335903797	29.023104504767446	21.636852019801477
30-31	21.927013835811376	27.477417045531087	28.97015307291658	21.625416045740955
32-33	21.81073408108285	27.607285800093965	28.878749278913347	21.703230839909843
34-35	21.908533206728823	27.622747359475653	28.881694397405887	21.58702503638964
36-37	21.83805344080314	27.55450595104466	28.889115917900497	21.718324690251702
38-39	21.686487805293957	27.663233122206215	28.936048450332724	21.7142306221671
40-41	22.05576800039349	27.457143913145828	28.87685391833999	21.610234168120698
42-43	21.771127133318714	27.495538651160327	28.80982177289466	21.923512442626297
44-45	21.897640817488394	27.569045713683206	28.67293285946284	21.860380609365556
46-47	21.898358137469263	27.568802170705336	28.625683622741242	21.907156069084156
48-49	21.81107432201225	27.746801035235602	28.57479673621252	21.867327906539625
50-51	21.85202531770821	27.842717530764844	28.317215991140394	21.98804116038655
52-53	21.94282702632611	27.674355037852155	28.236232192011045	22.14658574381069
54-55	22.026676973901836	27.3989731605004	28.51448691434701	22.059862951250754
56-57	21.90480555719368	27.263400135734038	28.612478539755536	22.219315767316747
58-59	21.78821297099745	27.087163055822554	28.63159926095443	22.493024712225566
60-61	21.911158352508156	27.244257023261447	28.939857305443855	21.904727318786538
62-63	21.540359069222877	27.453204412429944	28.913567739156914	22.092868779190265
64-65	21.41559348888594	27.701724463492145	28.610061171307144	22.272620876314768
66-67	21.47152761805605	28.17649151436586	28.2001811406785	22.15179972689959
68-69	21.562314618822807	28.161247387349942	28.04632050125811	22.23011749256914
70-71	21.555961997116142	27.687256926629626	28.059481626920153	22.697299449334075
72-73	21.69608385869515	27.323197542524376	28.047751731110253	22.932966867670217
74-75	21.79288764939828	27.395349676395007	28.133535065584457	22.67822760862225
76-77	22.134857588396688	27.30415534201706	27.6036788599175	22.957308209668746
78-79	22.26984963214932	27.401877065621242	27.199537977574984	23.128735324654453
80-81	22.149928388116756	27.662585595690654	27.331083529309108	22.85640248688348
82-83	22.114214884114197	27.507573029310784	27.389574479847205	22.988637606727814
84-85	22.213704983218328	27.791567138809715	27.33189393306531	22.662833944906644
86-87	22.510771098844245	27.72745052039155	27.390479168152993	22.371299212611213
88-89	22.351914268580682	27.86755935287384	27.60934481555262	22.17118156299286
90-91	22.363280120220082	27.54772162823334	27.504784310217	22.584213941329576
92-93	22.374075367687325	27.185916354670503	27.66138038467907	22.778627892963105
94-95	21.85088675155964	27.5547991811301	28.090811050229057	22.503503017081204
96-97	21.916072389555154	27.526400784654303	28.267961996497633	22.28956482929291
98-99	23.034420042448655	29.39991375801845	28.63969155576308	18.92597464376982
100	22.033363009627397	30.470882519123897	22.350289042207663	25.145465429041042
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	591.0
1	611.0
2	927.5
3	1363.0
4	1794.0
5	2494.0
6	3124.5
7	3928.0
8	5055.5
9	5834.0
10	5998.5
11	6102.0
12	6768.5
13	8141.5
14	10528.0
15	13283.0
16	15402.5
17	16633.5
18	16554.5
19	15395.0
20	14161.5
21	13444.0
22	14166.5
23	16903.5
24	21558.0
25	28373.5
26	37588.0
27	48732.5
28	60761.0
29	73941.5
30	86128.5
31	98732.0
32	113240.5
33	126867.5
34	139948.5
35	152322.5
36	165717.5
37	178649.5
38	188254.5
39	195919.0
40	205268.5
41	214253.5
42	218805.0
43	222797.0
44	225985.5
45	227444.0
46	226568.0
47	224776.5
48	222551.5
49	216755.0
50	210399.5
51	202352.5
52	190852.5
53	178112.5
54	165386.5
55	152306.0
56	138201.0
57	122880.5
58	107799.5
59	92780.0
60	76390.5
61	60937.0
62	47571.0
63	35841.5
64	26219.0
65	19202.5
66	14374.5
67	10919.5
68	8223.0
69	6046.5
70	4305.0
71	3091.5
72	2191.5
73	1522.0
74	995.0
75	603.5
76	363.5
77	223.5
78	141.0
79	79.5
80	54.5
81	38.5
82	21.0
83	12.0
84	9.0
85	7.0
86	4.0
87	1.5
88	0.5
89	0.0
90	0.5
91	1.0
92	1.5
93	2.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.002761790389006517
3	0.0
4	0.0
5	0.004726554021454107
6	3.336391073967605E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	5.671202935868336E-5
44-45	1.901515745738822E-5
46-47	0.0
48-49	0.0
50-51	0.0
52-53	9.428684878284912E-4
54-55	0.0023828753544162364
56-57	0.0025398397215167924
58-59	0.001762290393326664
60-61	0.004013117216204341
62-63	0.0017443636113136072
64-65	0.001786020637865364
66-67	1.0995173119000758E-4
68-69	9.066009616014199E-5
70-71	9.138326853582681E-5
72-73	0.0
74-75	0.0
76-77	0.0
78-79	8.380592015495715E-5
80-81	2.746575970502619E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	2.2036083425526795E-5
92-93	2.0015752397136547E-4
94-95	0.0
96-97	0.0
98-99	1.2359124519168261E-5
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	40.0
20-21	120.0
22-23	273.0
24-25	338.0
26-27	906.0
28-29	2419.0
30-31	7911.0
32-33	10048.0
34-35	13796.0
36-37	22058.0
38-39	14020.0
40-41	26354.0
42-43	29356.0
44-45	35426.0
46-47	36181.0
48-49	33797.0
50-51	17336.0
52-53	3116.0
54-55	2743.0
56-57	2746.0
58-59	19048.0
60-61	34971.0
62-63	34104.0
64-65	36126.0
66-67	38490.0
68-69	39176.0
70-71	38607.0
72-73	36830.0
74-75	36911.0
76-77	38971.0
78-79	39722.0
80-81	39435.0
82-83	37692.0
84-85	37980.0
86-87	38808.0
88-89	40810.0
90-91	41492.0
92-93	42158.0
94-95	52515.0
96-97	181277.0
98-99	418698.0
100-101	3812246.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.16630526700577
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.54126748572301	78.92147760922155
2	6.972588498499029	12.155495551227594
3	1.4794536365485138	3.868755219353087
4	0.5085940777754435	1.7732906656146235
5	0.20098087009201765	0.875937993763462
6	0.10968752931708123	0.5736633998661809
7	0.06268287055969471	0.38246839611529904
8	0.033090413381606815	0.23074952593860415
9	0.024316051086613753	0.19075862987134937
>10	0.06590537269428484	0.8611662595498655
>50	8.422900908202808E-4	0.04949998571091269
>100	5.303175230762517E-4	0.0775508568117754
>500	6.058670881440103E-5	0.03918590695570644
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8535505966486693E-5
2	0.0	1.8535505966486693E-5	0.0	0.0	1.8535505966486693E-5
3	0.0	1.8535505966486693E-5	0.0	0.0	1.8535505966486693E-5
4	0.0	1.1121303579892016E-4	0.0	0.0	1.8535505966486693E-5
5	0.0	1.1121303579892016E-4	0.0	0.0	1.8535505966486693E-5
6	9.267752983243347E-5	1.1121303579892016E-4	0.0	0.0	1.8535505966486693E-5
7	9.267752983243347E-5	1.1121303579892016E-4	0.0	0.0	1.8535505966486693E-5
8	1.1121303579892016E-4	1.2974854176540685E-4	0.0	1.8535505966486693E-5	1.8535505966486693E-5
9	1.1121303579892016E-4	2.594970835308137E-4	0.0	1.8535505966486693E-5	1.8535505966486693E-5
10-11	1.1121303579892016E-4	2.594970835308137E-4	0.0	1.8535505966486693E-5	1.8535505966486693E-5
12-13	1.1121303579892016E-4	2.594970835308137E-4	0.0	3.7071011932973386E-5	1.8535505966486693E-5
14-15	1.1121303579892016E-4	2.965680954637871E-4	0.0	3.7071011932973386E-5	4.6338764916216734E-5
16-17	1.2974854176540685E-4	2.965680954637871E-4	0.0	5.5606517899460075E-5	5.560651789946008E-5
18-19	1.2974854176540685E-4	3.058358484470304E-4	0.0	7.414202386594677E-5	8.340977684919012E-5
20-21	1.2974854176540685E-4	3.1510360143027376E-4	0.0	9.267752983243347E-5	9.267752983243347E-5
22-23	1.2974854176540685E-4	4.0778113126270726E-4	0.0	1.0194528281567682E-4	1.1121303579892015E-4
24-25	1.2974854176540685E-4	4.448521431956806E-4	0.0	1.1121303579892016E-4	1.2974854176540685E-4
26-27	1.2974854176540685E-4	4.633876491621673E-4	0.0	1.575518007151369E-4	1.2974854176540685E-4
28-29	1.2974854176540685E-4	4.633876491621673E-4	0.0	2.5022933054757036E-4	1.2974854176540685E-4
30-31	1.2974854176540685E-4	5.189941670616274E-4	0.0	9.453108042908214E-4	1.2974854176540685E-4
32-33	1.2974854176540685E-4	5.189941670616274E-4	0.0	0.002706183871107057	1.2974854176540685E-4
34-35	1.9462281264811027E-4	5.560651789946007E-4	0.0	0.0048470348102362705	1.2974854176540685E-4
36-37	2.40961577564327E-4	6.024039439108175E-4	0.0	0.010926680767243905	1.2974854176540685E-4
38-39	2.40961577564327E-4	6.209394498773042E-4	0.0	0.12886810523199874	1.6681955369838024E-4
40-41	2.40961577564327E-4	6.580104618102776E-4	0.0	0.25047955987811793	1.6681955369838024E-4
42-43	2.40961577564327E-4	7.136169797097376E-4	0.0	0.26502993206181	1.7608730668162357E-4
44-45	2.40961577564327E-4	7.784912505924411E-4	0.0	0.28306497936720154	1.8535505966486694E-4
46-47	2.40961577564327E-4	7.970267565589278E-4	0.0	0.3025272606320125	2.0389056563135363E-4
48-49	2.40961577564327E-4	8.433655214751445E-4	0.0	0.32147054772976197	2.0389056563135363E-4
50-51	2.8730034248054375E-4	0.0010379883341232548	0.0	0.3412108615840703	2.0389056563135363E-4
52-53	2.965680954637871E-4	0.0010565238400897414	0.0	0.7373238918408742	2.0389056563135363E-4
54-55	2.965680954637871E-4	0.0010565238400897414	0.0	1.2793854960777944	2.0389056563135363E-4
56-57	2.965680954637871E-4	0.0010750593460562281	0.0	1.8727533808299497	2.0389056563135363E-4
58-59	2.965680954637871E-4	0.0010843270990394714	0.0	2.4879560916106263	2.0389056563135363E-4
60-61	2.965680954637871E-4	0.0011399336169389317	0.0	2.6508646535500775	2.0389056563135363E-4
62-63	3.336391073967605E-4	0.001204807887821635	0.0	2.655544868806616	2.0389056563135363E-4
64-65	3.336391073967605E-4	0.0014086984534529886	0.0	2.659965586979623	2.0389056563135363E-4
66-67	3.336391073967605E-4	0.0014550372183692055	0.0	2.6645901957182607	2.0389056563135363E-4
68-69	3.336391073967605E-4	0.0015384469952183955	0.0	2.6689645751263518	2.0389056563135363E-4
70-71	3.799778723129772E-4	0.0015847857601346124	0.0	2.672745818343515	2.0389056563135363E-4
72-73	4.2631663722919393E-4	0.0016218567720675857	0.0	2.675600286262354	2.0389056563135363E-4
74-75	4.2631663722919393E-4	0.001658927784000559	0.0	2.678028437543964	2.0389056563135363E-4
76-77	4.2631663722919393E-4	0.0017330698078665057	0.0	2.6799561301644785	2.0389056563135363E-4
78-79	4.4485214319568066E-4	0.001770140819799479	0.0	2.6812258123231825	2.0389056563135363E-4
80-81	4.4485214319568066E-4	0.0017794085727827226	0.0	2.6817355387372612	2.0389056563135363E-4
82-83	4.54119896178924E-4	0.001788676325765966	0.0	2.682171123127474	2.0389056563135363E-4
84-85	4.8192315512865405E-4	0.0017979440787492093	0.0	2.6824769589759208	2.0389056563135363E-4
86-87	5.004586610951407E-4	0.0017979440787492093	0.0	2.6825603687527697	2.0389056563135363E-4
88	5.189941670616274E-4	0.0018535505966486693	0.0	2.682569636505753	2.0389056563135363E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5870	0.0	49.79558	1
ATCTCGT	3925	0.0	40.391098	94
GTATCAA	11210	0.0	39.505104	1
TATCTCG	4945	0.0	36.082844	94
TCAACGC	13050	0.0	33.19169	4
ATCAACG	13165	0.0	32.90175	3
CAACGCA	13340	0.0	32.437134	5
TATGCCG	2145	0.0	31.837143	86-87
AACGCAG	13820	0.0	31.309065	6
TATCAAC	14235	0.0	30.831776	2
ATGCCGT	2365	0.0	30.62223	88-89
TGCCGTC	2275	0.0	30.54627	88-89
GCCGTCT	2395	0.0	30.337112	90-91
TCTCGTA	2355	0.0	28.91264	82-83
CTCGTAT	2390	0.0	28.380909	82-83
CCGTCTT	2500	0.0	28.198627	90-91
TCGTATG	2490	0.0	28.121407	84-85
ACGCAGA	15740	0.0	27.489916	7
CGTATGC	2515	0.0	27.426321	84-85
CGCAGAG	16020	0.0	27.009441	8
>>END_MODULE