FastQCFastQC Report
Fri 27 May 2016
SRR2139378_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139378_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6217080
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71200.048.823111
ATCTCGT50700.038.96042694
GTATCAA123550.038.22521
TATGCCG27950.033.74261586-87
CCGTCTT30600.031.62876590-91
GCCGTCT31300.031.52433690-91
TATCTCG61000.031.46539394
TCAACGC149600.031.4506384
ATCAACG150300.031.3041613
ATGCCGT31400.030.81935988-89
TGCCGTC30750.030.7791688-89
CAACGCA154450.030.491455
TCTCGTA31300.029.46067282-83
AACGCAG161300.029.4128196
TATCAAC161150.029.3880942
TCGTATG32950.029.0166784-85
CTCGTAT32100.028.88783582-83
CGTATGC32800.028.35293884-85
ACGCAGA187750.025.0819667
CGCAGAG191750.024.5358568
Produced by FastQC (version 0.11.5)