##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139378_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6217080
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.378667799031057	31.0	18.0	33.0	18.0	33.0
2	30.099726881429866	32.0	29.0	33.0	25.0	33.0
3	31.777275023001152	33.0	31.0	33.0	29.0	33.0
4	31.605105933975434	33.0	31.0	33.0	29.0	34.0
5	32.675346786594346	33.0	33.0	34.0	32.0	34.0
6	36.410454747244685	38.0	37.0	38.0	34.0	38.0
7	37.01994634136926	38.0	38.0	38.0	36.0	38.0
8	37.18408770676909	38.0	38.0	38.0	36.0	38.0
9	37.2197341838934	38.0	38.0	38.0	36.0	38.0
10-11	37.275159158318694	38.0	38.0	38.0	36.5	38.0
12-13	37.293254711214914	38.0	38.0	38.0	37.0	38.0
14-15	37.31518397704389	38.0	38.0	38.0	37.0	38.0
16-17	37.32563735708725	38.0	38.0	38.0	37.0	38.0
18-19	37.327233202725395	38.0	38.0	38.0	37.0	38.0
20-21	37.29180785938007	38.0	38.0	38.0	37.0	38.0
22-23	37.30200947227482	38.0	38.0	38.0	37.0	38.0
24-25	37.323694934146715	38.0	38.0	38.0	37.0	38.0
26-27	37.300938012823366	38.0	38.0	38.0	37.0	38.0
28-29	37.278026706668705	38.0	38.0	38.0	37.0	38.0
30-31	37.276377713993696	38.0	38.0	38.0	37.0	38.0
32-33	37.215486533380506	38.0	38.0	38.0	36.5	38.0
34-35	37.22675575672609	38.0	38.0	38.0	37.0	38.0
36-37	37.22449320206559	38.0	38.0	38.0	37.0	38.0
38-39	37.217120523230534	38.0	38.0	38.0	37.0	38.0
40-41	37.2080957164964	38.0	38.0	38.0	37.0	38.0
42-43	37.183257173919415	38.0	38.0	38.0	36.0	38.0
44-45	37.16094478338316	38.0	38.0	38.0	36.0	38.0
46-47	37.13730291541388	38.0	38.0	38.0	36.0	38.0
48-49	37.09662043929526	38.0	38.0	38.0	36.0	38.0
50-51	37.06798133685544	38.0	38.0	38.0	36.0	38.0
52-53	37.03472654351069	38.0	38.0	38.0	36.0	38.0
54-55	37.010658639866335	38.0	38.0	38.0	36.0	38.0
56-57	36.98233009461964	38.0	38.0	38.0	36.0	38.0
58-59	36.99155996812565	38.0	38.0	38.0	36.0	38.0
60-61	37.010645318694046	38.0	38.0	38.0	36.0	38.0
62-63	37.0205901546727	38.0	38.0	38.0	36.0	38.0
64-65	37.016397802356735	38.0	38.0	38.0	36.0	38.0
66-67	37.022269897260614	38.0	38.0	38.0	36.0	38.0
68-69	37.02096955078267	38.0	38.0	38.0	36.0	38.0
70-71	37.01067637199856	38.0	38.0	38.0	36.0	38.0
72-73	36.99124132023604	38.0	38.0	38.0	36.0	38.0
74-75	36.979229080887634	38.0	38.0	38.0	36.0	38.0
76-77	36.97505765366128	38.0	38.0	38.0	36.0	38.0
78-79	36.94569315683573	38.0	38.0	38.0	36.0	38.0
80-81	36.91833406426276	38.0	38.0	38.0	35.5	38.0
82-83	36.90099539653352	38.0	38.0	38.0	35.0	38.0
84-85	36.86786290663746	38.0	38.0	38.0	35.0	38.0
86-87	36.85634990366805	38.0	38.0	38.0	35.0	38.0
88-89	36.859961683479696	38.0	38.0	38.0	35.0	38.0
90-91	36.841380315703304	38.0	38.0	38.0	35.0	38.0
92-93	36.84740021561379	38.0	38.0	38.0	35.0	38.0
94-95	36.776741315036986	38.0	38.0	38.0	35.0	38.0
96-97	36.71366730073234	38.0	38.0	38.0	34.0	38.0
98-99	36.80453711906469	38.0	38.0	38.0	35.0	38.0
100	35.181222526521516	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	11.0
19	44.0
20	97.0
21	222.0
22	515.0
23	1419.0
24	3276.0
25	6466.0
26	11293.0
27	18703.0
28	27790.0
29	40187.0
30	55169.0
31	73100.0
32	96253.0
33	130077.0
34	190225.0
35	334510.0
36	945025.0
37	4282698.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.6508650363193	21.640657672090434	14.277779922407305	24.430697369182962
2	13.770777698531193	22.084816794013463	41.20568799600829	22.938717511447056
3	17.89708351830763	26.548990844576554	30.717716355588152	24.836209281527662
4	12.564998359358412	18.40730696725794	39.466115925804395	29.561578747579247
5	13.180153123374872	38.15365644509079	35.600404515687046	13.06578591584729
6	29.45279502376281	38.60325134022201	19.021155795811755	12.922797840203426
7	26.069943779064094	33.03824834781736	23.48372925613459	17.408078616983957
8	24.23251751626165	36.751915690324076	21.418205974508933	17.59736081890534
9	25.612104074581637	16.915497307417628	21.287115494733865	36.18528312326687
10-11	23.8333429841662	26.879274514723956	29.357946174088156	19.929436327021687
12-13	24.812604952807426	24.430697369182962	29.299317364421885	21.45738031358773
14-15	21.9611618315994	25.803640937546245	26.883914956860778	25.35128227399358
16-17	21.585639882388516	28.943828935770487	27.533142568537	21.937388613303995
18-19	21.617037258648754	28.173933743815425	29.177145862687947	21.03188313484787
20-21	22.435494540183086	27.381616170146028	28.997086623197234	21.18580266647365
22-23	22.14967350261231	27.27596116888405	28.838754099062086	21.735611229441552
24-25	21.923944989422125	27.361591026867394	28.95350424317425	21.760959740536233
26-27	21.88444112254658	27.51349483022942	28.830300041433453	21.771764005790548
28-29	21.81408516070643	27.563046765635306	28.88485115519289	21.73801691846537
30-31	22.07512905382007	27.392581317999888	28.823826435698578	21.70846319248146
32-33	21.92461947143135	27.538583755658752	28.754850301063183	21.781946471846712
34-35	22.00473470219661	27.534913778112802	28.785404111766148	21.67494740792444
36-37	21.982638380209814	27.46781777725653	28.758109725339676	21.79143411719398
38-39	21.813690084970002	27.56608535814443	28.7931727432794	21.827051813606165
40-41	22.16202908710324	27.38582285987509	28.772040407372607	21.68010764564906
42-43	21.894978761716597	27.467955004060745	28.62559611326909	22.01147012095357
44-45	21.98679013184784	27.524038431687657	28.54023117062301	21.9489402658415
46-47	21.97214287894275	27.487822495199794	28.510045784783	22.029988841074452
48-49	21.90295751004375	27.74499475430565	28.407786471517205	21.94426126413339
50-51	21.917448711406994	27.7949952800417	28.16094106625824	22.126614942293067
52-53	22.006584582776753	27.6326427073348	28.091867282818317	22.26890542707013
54-55	22.141701013063273	27.3502498238313	28.40074258751973	22.107306575585696
56-57	21.969029237893007	27.167933413853913	28.48629316575734	22.376744182495738
58-59	21.857321443877996	27.01393279634456	28.507085213515417	22.621660546262028
60-61	21.99579253717107	27.187857635620205	28.801028369574883	22.015321457633846
62-63	21.645189920578495	27.411970648551982	28.769034528094174	22.173804902775345
64-65	21.505487441374274	27.61337909407394	28.48395139009273	22.39718207445905
66-67	21.580755517012964	28.132884626989412	28.031308317667303	22.25505153833032
68-69	21.67299989910112	28.134406078499325	27.8933016306136	22.299292391785954
70-71	21.680562190259526	27.689645626793634	27.869240174397596	22.760552008549244
72-73	21.861194534518763	27.251870835980334	27.85573496784225	23.03119966165865
74-75	21.87792189756046	27.326810591868	28.016627374592673	22.77864013597887
76-77	22.22283178184843	27.2434952506468	27.465223317842884	23.068449649661883
78-79	22.38392904962657	27.31007888354157	27.043098664725456	23.262893402106403
80-81	22.19254670817566	27.635928453363995	27.21309828710215	22.958426551358198
82-83	22.223319364359238	27.441112463876056	27.233521243859343	23.102046927905363
84-85	22.281159367316736	27.74025866493209	27.217740732341916	22.760841235409256
86-87	22.680590607043698	27.634024785305428	27.269163576043887	22.41622103160699
88-89	22.433399026117502	27.827624407782153	27.47440846892239	22.264568097177957
90-91	22.43708854749324	27.497346546306705	27.37513004550279	22.690434860697266
92-93	22.4538643908527	27.122446155690312	27.565099012476917	22.85859044098007
94-95	21.927774428120443	27.5016323297881	27.958626312173816	22.61196692991764
96-97	21.989456033930068	27.481126399368744	28.170883266755435	22.358534299945752
98-99	23.157461252802772	29.324209250753245	28.48639181935318	19.031937677090802
100	22.188866580332935	30.37126153559791	22.220328075752743	25.219543808316413
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	863.0
1	860.5
2	1187.0
3	1676.5
4	2094.5
5	2724.5
6	3398.5
7	4660.5
8	6171.0
9	7050.0
10	7225.5
11	7286.0
12	8039.5
13	9496.0
14	12236.5
15	15601.0
16	18024.0
17	19001.5
18	18525.0
19	17164.5
20	15906.0
21	15514.5
22	16497.5
23	19394.0
24	24681.5
25	32292.5
26	42343.0
27	54537.0
28	67731.0
29	82160.5
30	96223.0
31	110296.0
32	126204.5
33	141846.5
34	156766.5
35	171422.0
36	187084.5
37	202239.0
38	213715.5
39	222735.5
40	233754.5
41	243678.0
42	250132.0
43	255996.5
44	259250.0
45	262217.0
46	263582.5
47	262521.0
48	260287.5
49	254645.0
50	247811.0
51	238620.5
52	226252.0
53	212130.0
54	196816.0
55	180617.5
56	164287.0
57	146767.0
58	128757.0
59	111336.0
60	92097.0
61	73316.0
62	57246.0
63	43708.5
64	32386.5
65	23539.0
66	17380.0
67	13255.0
68	9881.5
69	7093.0
70	5146.5
71	3744.0
72	2693.5
73	1845.0
74	1211.5
75	788.0
76	482.5
77	264.5
78	164.0
79	103.5
80	70.0
81	48.5
82	32.5
83	26.0
84	21.5
85	16.0
86	11.5
87	9.5
88	4.5
89	1.0
90	0.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0031847748460692156
3	0.0
4	0.0
5	0.004230281739980827
6	2.0910137878232224E-4
7	1.608472144479402E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	6.581428393730004E-5
44-45	1.6557243980510468E-5
46-47	0.0
48-49	0.0
50-51	1.6909834311524974E-5
52-53	0.0010844838322947425
54-55	0.002348295650252815
56-57	0.0027058117613409562
58-59	0.0017567388544891508
60-61	0.004214451781723475
62-63	0.001666145638305547
64-65	0.0016946000445610644
66-67	2.0898639768491834E-4
68-69	2.4566622838641684E-4
70-71	2.121921920169584E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	1.1841079236039072E-4
80-81	3.213612790325813E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	2.866015769009829E-5
92-93	2.7943961372580404E-4
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	43.0
20-21	156.0
22-23	281.0
24-25	423.0
26-27	1315.0
28-29	3572.0
30-31	11113.0
32-33	13953.0
34-35	19008.0
36-37	29540.0
38-39	18194.0
40-41	33274.0
42-43	36322.0
44-45	42762.0
46-47	43344.0
48-49	40667.0
50-51	20750.0
52-53	3660.0
54-55	3207.0
56-57	3093.0
58-59	21499.0
60-61	39690.0
62-63	38379.0
64-65	40227.0
66-67	43005.0
68-69	43503.0
70-71	42773.0
72-73	40213.0
74-75	40434.0
76-77	42416.0
78-79	43809.0
80-81	43166.0
82-83	41044.0
84-85	40346.0
86-87	42584.0
88-89	44152.0
90-91	44985.0
92-93	45731.0
94-95	58054.0
96-97	210361.0
98-99	480647.0
100-101	4405385.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.97103500488129
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.9159041490446	77.30163343093062
2	7.225045065311399	12.422892044434619
3	1.6502885702193442	4.256310493154482
4	0.5874599445292464	2.0201815782035784
5	0.25476705540809785	1.0951293719290054
6	0.13499789015494873	0.6963545004057728
7	0.07404789828260622	0.44561821187042794
8	0.04203543202210507	0.28910636782541616
9	0.029343117181462014	0.22703923389238234
>10	0.0846550305773034	1.078334284642936
>50	9.129538685824617E-4	0.05218961195668797
>100	4.872719349171369E-4	0.08343557760653905
>500	5.562146537467096E-5	0.03177529314757763
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.608472144479402E-5	0.0	0.0	0.0
3	0.0	1.608472144479402E-5	0.0	0.0	0.0
4	0.0	6.433888577917607E-5	0.0	1.608472144479402E-5	0.0
5	0.0	8.042360722397009E-5	0.0	1.608472144479402E-5	0.0
6	1.4476249300314617E-4	8.042360722397009E-5	0.0	4.8254164334382056E-5	0.0
7	1.4476249300314617E-4	8.042360722397009E-5	0.0	6.433888577917607E-5	0.0
8	1.4476249300314617E-4	8.042360722397009E-5	0.0	6.433888577917607E-5	0.0
9	1.4476249300314617E-4	1.9301665733752822E-4	0.0	8.042360722397009E-5	0.0
10-11	1.6084721444794019E-4	2.0105901805992523E-4	0.0	9.650832866876411E-5	0.0
12-13	1.769319358927342E-4	2.0910137878232224E-4	0.0	9.650832866876411E-5	0.0
14-15	1.769319358927342E-4	2.6539790383910133E-4	0.0	9.650832866876411E-5	0.0
16-17	2.8952498600629234E-4	2.734402645614983E-4	0.0	1.2867777155835215E-4	0.0
18-19	2.8952498600629234E-4	3.0560970745108635E-4	0.0	1.5280485372554318E-4	8.04236072239701E-6
20-21	2.8952498600629234E-4	3.377791503406744E-4	0.0	1.8497429661513121E-4	1.608472144479402E-5
22-23	2.8952498600629234E-4	3.779909539526594E-4	0.0	2.2518610022711626E-4	1.608472144479402E-5
24-25	2.8952498600629234E-4	4.0211803611985047E-4	0.0	2.573555431167043E-4	2.4127082167191028E-5
26-27	2.8952498600629234E-4	4.182027575646445E-4	0.0	3.2169442889588037E-4	3.216944288958804E-5
28-29	2.8952498600629234E-4	4.182027575646445E-4	0.0	5.629652505677906E-4	3.216944288958804E-5
30-31	2.8952498600629234E-4	4.8254164334382056E-4	0.0	0.001592387423034608	3.216944288958804E-5
32-33	2.9756734672868937E-4	4.986263647886145E-4	0.0	0.0037155706537474182	4.8254164334382056E-5
34-35	3.0560970745108635E-4	5.227534469558056E-4	0.0	0.0064338885779176075	4.8254164334382056E-5
36-37	3.0560970745108635E-4	5.549228898453937E-4	0.0	0.013326191717011843	6.433888577917607E-5
38-39	3.0560970745108635E-4	6.031770541797758E-4	0.0	0.15367342868356204	6.433888577917607E-5
40-41	3.0560970745108635E-4	6.192617756245697E-4	0.0	0.29893454805149683	6.433888577917607E-5
42-43	3.0560970745108635E-4	6.353464970693637E-4	0.0	0.31503535421773565	6.433888577917607E-5
44-45	3.0560970745108635E-4	7.39897186460525E-4	0.0	0.3352699337952865	6.433888577917607E-5
46-47	3.0560970745108635E-4	8.20320793684495E-4	0.0	0.35663044387397297	8.042360722397009E-5
48-49	3.2169442889588037E-4	8.36405515129289E-4	0.0	0.3781678858885522	1.0455068939116112E-4
50-51	3.377791503406744E-4	9.489985652428472E-4	0.0	0.3991182355703964	1.1259305011355813E-4
52-53	3.377791503406744E-4	9.489985652428472E-4	0.0	0.8059732221557387	1.1259305011355813E-4
54-55	3.377791503406744E-4	9.811680081324352E-4	0.0	1.357285092036776	1.1259305011355813E-4
56-57	3.377791503406744E-4	0.0010213798117444202	0.0	1.9598428844409272	1.3672013228074916E-4
58-59	3.377791503406744E-4	0.0010696339760788024	0.0	2.5734267533954847	1.769319358927342E-4
60-61	3.538638717854684E-4	0.0011178881404131842	0.0	2.7343463490899262	1.9301665733752822E-4
62-63	4.0211803611985047E-4	0.0011420152225803753	0.0	2.7394773752308157	2.0910137878232224E-4
64-65	4.1016039684224745E-4	0.0012867777155835215	0.0	2.7442143256963076	2.0910137878232224E-4
66-67	4.262451182870415E-4	0.0013350318799179035	0.0	2.7488145560295187	2.0910137878232224E-4
68-69	4.342874790094385E-4	0.0013591589620850946	0.0	2.7535917182986225	2.1714373950471925E-4
70-71	4.423298397318355E-4	0.0013993707656970796	0.0	2.7573394583952595	2.2518610022711626E-4
72-73	4.6645692189902654E-4	0.0014234978478642707	0.0	2.7599290985478713	2.4127082167191028E-4
74-75	4.6645692189902654E-4	0.001471752012198653	0.0	2.762269425518089	2.4127082167191028E-4
76-77	5.066687255110116E-4	0.001568260340867417	0.0	2.7644649899953033	2.4127082167191028E-4
78-79	5.147110862334086E-4	0.00164064158736899	0.0	2.765920657286057	2.4127082167191028E-4
80-81	5.147110862334086E-4	0.00164064158736899	0.0	2.7664514530937354	2.573555431167043E-4
82-83	5.147110862334086E-4	0.0016486839480913868	0.0	2.7667972746047984	2.8952498600629234E-4
84-85	5.147110862334086E-4	0.001704980473148166	0.0	2.7669983336228583	3.0560970745108635E-4
86-87	5.147110862334086E-4	0.001785404080372136	0.0	2.767054630147915	3.2169442889588037E-4
88	5.147110862334086E-4	0.0018658276875961062	0.0	2.76707071486936	3.2169442889588037E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7120	0.0	48.82311	1
ATCTCGT	5070	0.0	38.960426	94
GTATCAA	12355	0.0	38.2252	1
TATGCCG	2795	0.0	33.742615	86-87
CCGTCTT	3060	0.0	31.628765	90-91
GCCGTCT	3130	0.0	31.524336	90-91
TATCTCG	6100	0.0	31.465393	94
TCAACGC	14960	0.0	31.450638	4
ATCAACG	15030	0.0	31.304161	3
ATGCCGT	3140	0.0	30.819359	88-89
TGCCGTC	3075	0.0	30.77916	88-89
CAACGCA	15445	0.0	30.49145	5
TCTCGTA	3130	0.0	29.460672	82-83
AACGCAG	16130	0.0	29.412819	6
TATCAAC	16115	0.0	29.388094	2
TCGTATG	3295	0.0	29.01667	84-85
CTCGTAT	3210	0.0	28.887835	82-83
CGTATGC	3280	0.0	28.352938	84-85
ACGCAGA	18775	0.0	25.081966	7
CGCAGAG	19175	0.0	24.535856	8
>>END_MODULE