FastQCFastQC Report
Fri 27 May 2016
SRR2139377_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139377_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9039253
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101250.050.030571
ATCTCGT77900.042.8436494
GTATCAA183100.035.9818461
TATCTCG94550.035.37117494
TATGCCG41150.034.9830586-87
GCCGTCT46450.032.747890-91
ATGCCGT46300.032.30050388-89
CCGTCTT46950.032.15172690-91
TGCCGTC46300.031.81202588-89
TCGTATG46950.030.99009984-85
TCTCGTA47050.030.4278982-83
CTCGTAT46000.030.42042782-83
CGTATGC46750.030.30519584-85
TCAACGC215600.029.7024064
ATCAACG217500.029.4034943
CAACGCA221650.028.8529665
TATCAAC229850.028.1968462
AACGCAG229650.027.9785966
CGCTATC147450.025.50478794
ACGCAGA264500.024.2759767
Produced by FastQC (version 0.11.5)