##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139377_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9039253
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07887355293629	33.0	31.0	34.0	30.0	34.0
2	32.22163656664992	33.0	31.0	34.0	30.0	34.0
3	32.12414775866988	33.0	31.0	34.0	30.0	34.0
4	35.999363221717545	37.0	35.0	37.0	35.0	37.0
5	35.87508093865721	37.0	35.0	37.0	35.0	37.0
6	35.926730671218074	37.0	35.0	37.0	35.0	37.0
7	35.88437097623	37.0	35.0	37.0	35.0	37.0
8	35.876214992544185	37.0	35.0	37.0	35.0	37.0
9	37.63704301671831	39.0	37.0	39.0	35.0	39.0
10-11	37.51309538520495	39.0	37.0	39.0	35.0	39.0
12-13	37.49233758586025	39.0	37.0	39.0	35.0	39.0
14-15	38.78941246583097	40.0	38.0	41.0	35.0	41.0
16-17	38.64162293056738	40.0	38.0	41.0	34.0	41.0
18-19	38.716241596512454	40.0	38.0	41.0	34.0	41.0
20-21	38.746868631365146	40.0	38.0	41.0	34.0	41.0
22-23	38.67246945944085	40.0	38.0	41.0	34.0	41.0
24-25	38.61969836653943	40.0	38.0	41.0	34.0	41.0
26-27	38.42075789068608	40.0	38.0	41.0	34.0	41.0
28-29	38.31242391667685	40.0	38.0	41.0	34.0	41.0
30-31	38.18550316880881	40.0	38.0	41.0	33.5	41.0
32-33	37.99700047999886	40.0	37.0	41.0	33.0	41.0
34-35	38.083104894477735	40.0	37.5	41.0	33.0	41.0
36-37	38.06031954238279	40.0	37.0	41.0	33.0	41.0
38-39	38.01820596571284	40.0	37.0	41.0	33.0	41.0
40-41	37.94585700938619	40.0	37.0	41.0	33.0	41.0
42-43	38.005873509269605	40.0	37.0	41.0	33.0	41.0
44-45	38.01254015772493	40.0	37.0	41.0	33.0	41.0
46-47	37.95177621266586	40.0	37.0	41.0	33.0	41.0
48-49	37.86390130722542	40.0	37.0	41.0	33.0	41.0
50-51	37.7519799342784	40.0	37.0	41.0	32.5	41.0
52-53	37.58559270233734	40.0	36.5	41.0	32.0	41.0
54-55	37.44711938091419	39.0	36.0	41.0	32.0	41.0
56-57	37.31874117250277	39.0	36.0	41.0	31.0	41.0
58-59	37.1606856303094	39.0	35.5	41.0	31.0	41.0
60-61	36.975620752422074	39.0	35.0	40.5	31.0	41.0
62-63	36.775823444575366	38.5	35.0	40.0	31.0	41.0
64-65	36.484721897562906	38.0	35.0	40.0	31.0	41.0
66-67	36.19196539448154	37.5	35.0	40.0	30.0	41.0
68-69	35.87351733605364	37.0	34.0	40.0	30.0	41.0
70-71	35.55977088534364	36.5	34.0	39.0	30.0	41.0
72-73	35.2602886141551	36.0	34.0	39.0	29.0	40.5
74-75	34.911324681158625	35.5	34.0	38.5	29.0	40.0
76-77	33.61998228149441	34.5	32.0	36.5	28.0	39.0
78-79	34.2845897278018	35.0	33.0	37.0	29.0	39.0
80-81	34.17389299206182	35.0	33.5	37.0	29.0	39.0
82-83	33.934482269965144	35.0	33.0	36.0	29.0	38.0
84-85	33.70603626417097	35.0	33.0	36.0	29.0	37.0
86-87	33.50376488828303	35.0	33.0	35.5	29.0	37.0
88-89	33.33678782482312	35.0	33.0	35.0	29.0	36.5
90-91	33.221066513988305	35.0	33.0	35.0	29.0	36.0
92-93	33.17638395605962	35.0	33.0	35.0	29.0	36.0
94-95	33.08959375534306	35.0	33.0	35.0	29.0	36.0
96-97	33.12299632183286	35.0	33.0	35.0	29.0	35.0
98-99	33.245756921228356	35.0	33.0	35.0	29.0	35.0
100	32.04481159231187	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	5.0
20	34.0
21	302.0
22	1327.0
23	4772.0
24	12581.0
25	27917.0
26	51118.0
27	81041.0
28	117898.0
29	156945.0
30	199429.0
31	246754.0
32	303428.0
33	380665.0
34	504729.0
35	692930.0
36	1061990.0
37	1822307.0
38	2302774.0
39	1030874.0
40	39432.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.00249213071036	20.523864084786652	14.706779420821611	23.76686436368138
2	14.34427158969884	21.50615764377875	41.542780139022554	22.606790627499862
3	17.701377651596825	26.70446342567276	31.001171228987268	24.592987693743147
4	12.471495155628459	18.595010008017255	39.419053764730336	29.51444107162395
5	13.010676877834928	38.152887191010144	35.85890338504741	12.977532546107515
6	29.29425694800223	38.48985087595181	19.206819413064334	13.00907276298163
7	26.035281897740887	32.99300285100992	23.588796552104473	17.38291869914472
8	24.240045056820513	36.71475950501662	21.485447967879647	17.55974747028322
9	25.615523760647037	16.954874479119017	21.313719175688522	36.115882584545425
10-11	23.91145595769916	26.846582344802165	29.39482388644283	19.84713781105585
12-13	24.878239385489046	24.47075549273817	29.247599331493433	21.40340579027935
14-15	21.981346246199767	25.86502446607037	26.904568331033552	25.24906095669631
16-17	21.622897001109063	28.966282874266735	27.467141636266586	21.94367848835762
18-19	21.600882451680974	28.225249763287234	29.111717415880467	21.06215036915132
20-21	22.467387869942677	27.42496709801384	28.931167805882264	21.176477226161218
22-23	22.172375508565658	27.313843380781112	28.778911891475605	21.73486921917763
24-25	21.9386996254423	27.430458492884995	28.861803193312983	21.769038688359718
26-27	21.875782130870352	27.633394677582302	28.720789444736837	21.770033746810512
28-29	21.82079357745676	27.735073414593593	28.74712509862699	21.69700790932265
30-31	22.10778180655066	27.540697192745196	28.651362702573753	21.7001582981304
32-33	21.908280891895103	27.706258414387953	28.59252946898235	21.7929312247346
34-35	22.038805070742235	27.698685708108933	28.594083940704873	21.66842528044396
36-37	22.027690056654592	27.658900606660524	28.533891964652984	21.779517372031897
38-39	21.835200375097372	27.77789037905418	28.5622765005415	21.82463274530695
40-41	22.182850628090783	27.58513846179453	28.53889598665822	21.69311492345647
42-43	21.955196001520605	27.65563954175572	28.331109699424932	22.058054757298745
44-45	22.018616432961654	27.74095894276564	28.188227388691317	22.05219723558138
46-47	22.069959510746433	27.725329137080696	28.125999323262498	22.078712028910374
48-49	21.971473256925666	27.97610451248498	28.01210962904473	22.040312601544628
50-51	21.981451382444373	28.058469964285383	27.78237244056756	22.177706212702684
52-53	22.0286808394624	27.832372682399605	27.686906834440084	22.452039643697912
54-55	22.191578059328283	27.532618536857868	28.03032822083558	22.245475182978268
56-57	22.025407112345214	27.350857902494774	28.113233042496887	22.510501942663126
58-59	21.899934917620097	27.177451165281962	28.14457914287828	22.77803477421966
60-61	22.028615376938816	27.41329793154802	28.456471924861727	22.10161476665144
62-63	21.664877752417937	27.608202795424774	28.391971669841304	22.334947782315986
64-65	21.479983993589624	27.90154934876633	28.073707347511057	22.54475931013299
66-67	21.55211441534851	28.444734581754727	27.612450915352483	22.390700087544285
68-69	21.64989367566162	28.466416067231325	27.45169988291258	22.43199037419448
70-71	21.656678625508146	27.9539825455368	27.45742882813825	22.931910000816806
72-73	21.854722722124187	27.468893210703875	27.44838672590875	23.227997341263183
74-75	21.903549166686265	27.507110387211476	27.634454293361475	22.954886152740787
76-77	22.20378835585718	27.414140028205242	27.079003794569033	23.303067821368543
78-79	22.421731761694883	27.48069697793294	26.634599815268565	23.46297144510361
80-81	22.244846851604496	27.790078707655773	26.8153438102737	23.149730630466024
82-83	22.192043122870466	27.592175945100678	26.91095190669877	23.304829025330083
84-85	22.298632152260744	27.89367227395848	26.871316837891506	22.936378735889267
86-87	22.7219224126104	27.760408843368694	26.964015517724498	22.553653226296404
88-89	22.435438962584257	27.92137553461792	27.256406375069954	22.38677912772787
90-91	22.47671477117049	27.480306812989337	27.18455417087159	22.85842424496859
92-93	22.43429802556657	27.07629838571961	27.4215226167875	23.06788097192632
94-95	21.73426379450045	27.506259443913194	27.996744359200836	22.762732402385524
96-97	21.776727403013357	27.431646312532465	28.263456177710218	22.52817010674396
98-99	22.95043476076922	29.356381558423706	28.591411128500955	19.101772552306123
100	22.083456393501013	30.542243506610866	22.165253568597926	25.2090465312902
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1419.0
1	1286.0
2	1862.0
3	2734.0
4	3606.0
5	5760.5
6	7733.5
7	9260.0
8	10438.0
9	10583.5
10	9994.5
11	9281.0
12	9931.0
13	12925.5
14	19900.5
15	29415.5
16	37792.0
17	43546.5
18	45785.0
19	44796.0
20	42033.5
21	38861.5
22	37630.5
23	39787.5
24	46129.5
25	56523.5
26	71156.0
27	88576.0
28	107192.5
29	128312.5
30	148965.0
31	169502.0
32	191315.5
33	212290.5
34	233314.0
35	253414.5
36	274390.5
37	294595.0
38	310362.5
39	323505.5
40	338052.0
41	351594.5
42	360748.5
43	369265.5
44	375165.5
45	380755.5
46	383368.5
47	382301.5
48	380613.0
49	373493.0
50	364094.0
51	351676.5
52	334704.0
53	315608.0
54	295006.0
55	271904.0
56	247248.5
57	221304.0
58	194494.0
59	168303.5
60	140710.0
61	114447.5
62	90858.5
63	70047.0
64	52685.0
65	39059.5
66	29469.0
67	22736.0
68	17541.5
69	12902.0
70	9323.0
71	6772.0
72	4745.5
73	3287.5
74	2211.5
75	1459.0
76	967.0
77	619.5
78	410.0
79	275.0
80	185.5
81	126.0
82	87.0
83	63.0
84	51.5
85	38.0
86	25.5
87	18.5
88	13.0
89	9.5
90	7.0
91	5.5
92	4.5
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	3.31885831716404E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	8.629031624626505E-4
18-19	0.0038111556342100396
20-21	0.0
22-23	0.0013332356733592515
24-25	5.311905108127148E-4
26-27	0.013821443773514306
28-29	0.007659170777079087
30-31	6.765305561125534E-4
32-33	0.0069823147307272555
34-35	0.004774188150978123
36-37	0.0020224970016902138
38-39	0.0015426136897844407
40-41	0.0036797467700173552
42-43	0.008345061386397047
44-45	0.009687269736791014
46-47	0.008490441342665153
48-49	0.007577907837520045
50-51	0.03446483824159828
52-53	0.01304889547413793
54-55	5.3439886925949474E-5
56-57	0.008307846323754213
58-59	0.006021812541749894
60-61	1.0195435095890166E-4
62-63	0.004954229333030071
64-65	0.002208826078498994
66-67	0.021969028535347594
68-69	0.03042600385910198
70-71	0.013846044052030972
72-73	0.010188755902150893
74-75	0.015287646421982737
76-77	0.0020580728619923644
78-79	0.002367289551555577
80-81	0.0014614097882979296
82-83	0.02297469225617096
84-85	7.848960557416939E-4
86-87	0.008787006790719386
88-89	8.328528813274176E-4
90-91	0.002796354005174875
92-93	0.0010024039795437987
94-95	7.737561759977039E-4
96-97	0.0026233695758086347
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	157.0
20-21	635.0
22-23	1403.0
24-25	3056.0
26-27	3809.0
28-29	8553.0
30-31	21432.0
32-33	27196.0
34-35	36610.0
36-37	51907.0
38-39	36674.0
40-41	65368.0
42-43	70639.0
44-45	81560.0
46-47	80421.0
48-49	72264.0
50-51	40650.0
52-53	12968.0
54-55	12826.0
56-57	13141.0
58-59	42701.0
60-61	72239.0
62-63	70620.0
64-65	74572.0
66-67	78713.0
68-69	80292.0
70-71	78438.0
72-73	77265.0
74-75	78627.0
76-77	78034.0
78-79	83597.0
80-81	85970.0
82-83	85818.0
84-85	87584.0
86-87	94302.0
88-89	102620.0
90-91	112619.0
92-93	129125.0
94-95	154430.0
96-97	377402.0
98-99	681671.0
100-101	5671345.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.81949527613288
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.4035827130999	75.83166761603131
2	7.390120456746395	12.536525743421079
3	1.834530843975214	4.668119407634272
4	0.6495485956219688	2.203775361519053
5	0.2941081211183978	1.2473051194937133
6	0.141453380172789	0.7198802586815343
7	0.0814191484893069	0.48341517564728703
8	0.05428554405907043	0.3683577958304502
9	0.032216101561028845	0.24592981267539932
>10	0.11652152396454923	1.4745597554010252
>50	0.0017471290630007039	0.09464867942333131
>100	3.635537514422216E-4	0.050974198198637966
>500	8.23115128955597E-5	0.050790577046849424
>1k	2.0576864137174547E-5	0.02405049899602411
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.2125722114426932E-5
2	0.0	1.1062861057213466E-5	0.0	4.4251444228853864E-5	2.2125722114426932E-5
3	0.0	1.1062861057213466E-5	0.0	4.4251444228853864E-5	2.2125722114426932E-5
4	0.0	4.4251444228853864E-5	0.0	4.4251444228853864E-5	2.2125722114426932E-5
5	0.0	5.531430528606733E-5	0.0	6.63771663432808E-5	3.31885831716404E-5
6	1.1062861057213466E-5	5.531430528606733E-5	0.0	6.63771663432808E-5	3.31885831716404E-5
7	1.1062861057213466E-5	5.531430528606733E-5	0.0	9.95657495149212E-5	3.31885831716404E-5
8	1.1062861057213466E-5	6.63771663432808E-5	0.0	9.95657495149212E-5	3.31885831716404E-5
9	1.1062861057213466E-5	1.5488005480098854E-4	0.0	1.7700577691541545E-4	4.4251444228853864E-5
10-11	1.6594291585820197E-5	1.5488005480098854E-4	0.0	2.0466292955844913E-4	4.4251444228853864E-5
12-13	2.2125722114426932E-5	1.5488005480098854E-4	0.0	2.1572579061566262E-4	4.4251444228853864E-5
14-15	2.2125722114426932E-5	1.9913149902984242E-4	0.0	2.3785151273008953E-4	4.4251444228853864E-5
16-17	3.31885831716404E-5	2.1019436008705588E-4	0.0	2.765715264303367E-4	5.531430528606733E-5
18-19	3.31885831716404E-5	2.2125722114426933E-4	0.0	4.0932585911689827E-4	7.190859687188753E-5
20-21	3.31885831716404E-5	2.323200822014828E-4	0.0	4.97828747574606E-4	7.744002740049427E-5
22-23	3.31885831716404E-5	2.5997723484451644E-4	0.0	7.688688434763359E-4	8.2971457929101E-5
24-25	3.31885831716404E-5	2.986972485447636E-4	0.0	0.0019415321155409635	8.850288845770773E-5
26-27	3.31885831716404E-5	3.208229706591905E-4	0.0	0.005227201849533363	9.403431898631447E-5
28-29	3.31885831716404E-5	3.208229706591905E-4	0.0	0.015980302797144852	1.0509718004352792E-4
30-31	3.31885831716404E-5	3.429486927736175E-4	0.0	0.018176280717001725	1.1062861057213467E-4
32-33	3.8720013700247136E-5	3.429486927736175E-4	0.0	0.02307712816534729	1.4381719374377506E-4
34-35	4.4251444228853864E-5	3.9826299805968484E-4	0.0	0.028663872999240093	1.5488005480098854E-4
36-37	4.4251444228853864E-5	4.867658865173925E-4	0.0	0.04059516864944482	1.65942915858202E-4
38-39	4.97828747574606E-5	5.254859002176396E-4	0.0	0.19484464037017218	1.7147434638680874E-4
40-41	5.531430528606733E-5	5.642059139178868E-4	0.0	0.35393411380343043	1.7700577691541545E-4
42-43	5.531430528606733E-5	6.084573581467407E-4	0.0	0.3799097115657677	1.9360006850123565E-4
44-45	5.531430528606733E-5	6.361145107897743E-4	0.0	0.41497344968660577	2.2125722114426933E-4
46-47	5.531430528606733E-5	6.527088023755946E-4	0.0	0.45237698292104445	2.4338294325869624E-4
48-49	6.63771663432808E-5	6.527088023755946E-4	0.0	0.4880934298442582	2.986972485447636E-4
50-51	8.850288845770773E-5	7.080231076616618E-4	0.0	0.5215309273896858	2.986972485447636E-4
52-53	8.850288845770773E-5	7.080231076616618E-4	0.0	0.9756613737882986	3.152915401305838E-4
54-55	8.850288845770773E-5	7.135545381902685E-4	0.0	1.5835545260211212	3.31885831716404E-4
56-57	8.850288845770773E-5	7.190859687188754E-4	0.0	2.2482665326437923	3.3741726224501077E-4
58-59	8.850288845770773E-5	7.246173992474821E-4	0.0	2.9263756640067493	3.484801233022242E-4
60-61	8.850288845770773E-5	7.46743121361909E-4	0.0	3.1077733967618784	3.540115538308309E-4
62-63	9.95657495149212E-5	7.633374129477292E-4	0.0	3.1157441881536005	3.540115538308309E-4
64-65	9.95657495149212E-5	8.186517182337965E-4	0.0	3.1232116193672197	3.540115538308309E-4
66-67	9.95657495149212E-5	8.463088708768302E-4	0.0	3.128958775686442	3.7060584541665116E-4
68-69	9.95657495149212E-5	8.629031624626505E-4	0.0	3.134125131800161	3.761372759452579E-4
70-71	1.1062861057213467E-4	8.794974540484706E-4	0.0	3.1383234875713732	3.761372759452579E-4
72-73	1.5488005480098854E-4	8.850288845770773E-4	0.0	3.142035077456068	3.982629980596848E-4
74-75	1.5488005480098854E-4	9.016231761628975E-4	0.0	3.1451603357047313	3.982629980596848E-4
76-77	1.5488005480098854E-4	9.514060509203581E-4	0.0	3.1479703024132633	3.982629980596848E-4
78-79	1.5488005480098854E-4	9.735317730347851E-4	0.0	3.149895240237219	3.982629980596848E-4
80-81	1.5488005480098854E-4	9.735317730347851E-4	0.0	3.1504594461511366	3.982629980596848E-4
82-83	1.5488005480098854E-4	9.735317730347851E-4	0.0	3.150990463481883	3.982629980596848E-4
84-85	1.5488005480098854E-4	9.845946340919986E-4	0.0	3.1511342806756266	3.982629980596848E-4
86-87	1.6041148532959526E-4	9.95657495149212E-4	0.0	3.151211720703027	3.982629980596848E-4
88	1.8806863797262894E-4	0.0010841603836069197	0.0	3.1512338464251415	4.0932585911689827E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10125	0.0	50.03057	1
ATCTCGT	7790	0.0	42.84364	94
GTATCAA	18310	0.0	35.981846	1
TATCTCG	9455	0.0	35.371174	94
TATGCCG	4115	0.0	34.98305	86-87
GCCGTCT	4645	0.0	32.7478	90-91
ATGCCGT	4630	0.0	32.300503	88-89
CCGTCTT	4695	0.0	32.151726	90-91
TGCCGTC	4630	0.0	31.812025	88-89
TCGTATG	4695	0.0	30.990099	84-85
TCTCGTA	4705	0.0	30.42789	82-83
CTCGTAT	4600	0.0	30.420427	82-83
CGTATGC	4675	0.0	30.305195	84-85
TCAACGC	21560	0.0	29.702406	4
ATCAACG	21750	0.0	29.403494	3
CAACGCA	22165	0.0	28.852966	5
TATCAAC	22985	0.0	28.196846	2
AACGCAG	22965	0.0	27.978596	6
CGCTATC	14745	0.0	25.504787	94
ACGCAGA	26450	0.0	24.275976	7
>>END_MODULE