FastQCFastQC Report
Fri 27 May 2016
SRR2139376_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139376_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8091797
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95900.050.5321271
ATCTCGT63300.041.48592494
GTATCAA168100.036.8189851
TATCTCG71500.032.12534394
TATGCCG33750.030.72159286-87
TCAACGC196700.030.6750564
ATCAACG198800.030.3944783
CAACGCA203250.029.6865125
AACGCAG208450.029.0081126
TATCAAC210450.028.8555742
GCCGTCT38750.028.46275990-91
ATGCCGT37600.028.44718488-89
CCGTCTT37850.028.38463890-91
TGCCGTC37700.028.22395788-89
CTCGTAT36200.027.89452682-83
CGTATGC37100.027.73797684-85
TCGTATG38400.027.14970284-85
TCTCGTA39450.026.00065482-83
ACGCAGA243250.024.91147
CGCAGAG245650.024.6504348
Produced by FastQC (version 0.11.5)