##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139376_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8091797
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05084692559638	33.0	31.0	34.0	30.0	34.0
2	32.23159107921269	33.0	31.0	34.0	30.0	34.0
3	32.250202272746094	34.0	31.0	34.0	30.0	34.0
4	35.991754241981106	37.0	35.0	37.0	35.0	37.0
5	35.85896865677673	37.0	35.0	37.0	35.0	37.0
6	35.878459012256485	37.0	35.0	37.0	35.0	37.0
7	35.82378104641033	37.0	35.0	37.0	35.0	37.0
8	35.81083830946328	37.0	35.0	37.0	33.0	37.0
9	37.58713707227208	39.0	37.0	39.0	35.0	39.0
10-11	37.46550834134865	39.0	37.0	39.0	34.5	39.0
12-13	37.44585714396938	39.0	37.0	39.0	34.0	39.0
14-15	38.69515275778669	40.0	38.0	41.0	34.0	41.0
16-17	38.65151659143204	40.0	38.0	41.0	34.0	41.0
18-19	38.66730121875277	40.0	38.0	41.0	34.0	41.0
20-21	38.649980263747835	40.0	38.0	41.0	34.0	41.0
22-23	38.5715169100644	40.0	38.0	41.0	34.0	41.0
24-25	38.51279256900479	40.0	38.0	41.0	34.0	41.0
26-27	38.343828484004455	40.0	38.0	41.0	34.0	41.0
28-29	38.236279055459605	40.0	38.0	41.0	33.5	41.0
30-31	38.08701454547506	40.0	37.5	41.0	33.0	41.0
32-33	37.916457600586554	40.0	37.0	41.0	33.0	41.0
34-35	38.00564893909116	40.0	37.0	41.0	33.0	41.0
36-37	37.98228099135385	40.0	37.0	41.0	33.0	41.0
38-39	37.946959201132856	40.0	37.0	41.0	33.0	41.0
40-41	37.87342320800306	40.0	37.0	41.0	33.0	41.0
42-43	37.77115699967207	40.0	37.0	41.0	33.0	41.0
44-45	37.83390537496453	40.0	37.0	41.0	33.0	41.0
46-47	37.832674952870214	40.0	37.0	41.0	33.0	41.0
48-49	37.77871088788708	40.0	37.0	41.0	33.0	41.0
50-51	37.69129462663491	40.0	37.0	41.0	32.0	41.0
52-53	37.510151102087534	40.0	36.0	41.0	32.0	41.0
54-55	37.39136652018965	39.0	36.0	41.0	31.5	41.0
56-57	37.23058485955906	39.0	36.0	41.0	31.0	41.0
58-59	37.10319482891869	39.0	35.0	41.0	31.0	41.0
60-61	36.889081970810544	39.0	35.0	40.5	31.0	41.0
62-63	36.687929298253614	38.5	35.0	40.0	31.0	41.0
64-65	36.397422471741564	38.0	35.0	40.0	30.5	41.0
66-67	36.12147516683258	37.5	34.5	40.0	30.0	41.0
68-69	35.81700718664457	37.0	34.0	40.0	30.0	41.0
70-71	35.521639526665496	36.5	34.0	39.0	29.0	41.0
72-73	35.23054802251794	36.0	34.0	39.0	29.0	40.5
74-75	34.84892495838126	35.5	34.0	38.5	29.0	40.0
76-77	33.54742656244438	34.5	31.5	36.5	27.5	39.0
78-79	34.20073293373366	35.0	33.0	37.0	29.0	39.0
80-81	34.10306373775556	35.0	33.0	36.5	29.0	39.0
82-83	33.817307851243335	35.0	33.0	36.0	29.0	38.0
84-85	33.64893651859086	35.0	33.0	36.0	29.0	37.0
86-87	33.412571088701085	35.0	33.0	35.0	29.0	37.0
88-89	33.313476417885425	35.0	33.0	35.0	29.0	36.5
90-91	33.193948339284404	35.0	33.0	35.0	29.0	36.0
92-93	33.076386186517674	35.0	33.0	35.0	29.0	36.0
94-95	33.027024613466175	35.0	33.0	35.0	29.0	36.0
96-97	33.082302376898426	35.0	33.0	35.0	29.0	35.0
98-99	33.218319981640306	35.0	33.0	35.0	29.0	35.0
100	32.06672135237331	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	35.0
21	244.0
22	1188.0
23	4475.0
24	12398.0
25	26669.0
26	49449.0
27	77947.0
28	111894.0
29	149653.0
30	188977.0
31	231868.0
32	284637.0
33	355709.0
34	464558.0
35	632027.0
36	956209.0
37	1618810.0
38	2031186.0
39	863326.0
40	30533.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.16927303045294	20.336310957874993	14.69766233631417	23.796753675357895
2	14.56804959392827	21.435226316231116	41.29929359325252	22.69743049658809
3	17.85421705462952	26.441370192554263	30.830741305052513	24.873671447763705
4	12.475177021865477	18.612713591307344	39.2412216964909	29.670887690336272
5	13.065836921020137	38.074842955155695	35.793198964333875	13.066121159490285
6	29.56190077432738	38.39052067173707	19.061971030662285	12.98560752327326
7	26.356753635811675	32.867038063362195	23.31981388064975	17.456394420176384
8	24.423487143832205	36.64874440127453	21.29161173964201	17.63615671525126
9	25.629572763627163	16.917601862725917	21.153175246487276	36.29965012715964
10-11	23.905944501573632	26.8040770177502	29.285361459265474	20.004617021410695
12-13	24.917943119051884	24.409489834487292	29.185469405399576	21.487097641061244
14-15	22.039807721325683	25.85316339497889	26.805683583016233	25.301345300679195
16-17	21.629365271654493	28.953307401892168	27.449424699058167	21.967902627395173
18-19	21.635732066350972	28.219121707608473	29.053924917206498	21.091221308834058
20-21	22.478384635676505	27.395109537145935	28.92162657358326	21.2048792535943
22-23	22.16637185467786	27.301878865583408	28.76380312780461	21.767946151934122
24-25	21.972143551304075	27.44945263873365	28.768612390882865	21.809791419079406
26-27	21.92248110719473	27.653234290821104	28.655015059888	21.76926954209617
28-29	21.85072045745735	27.726050310556623	28.711070899033242	21.71215833295279
30-31	22.162124958942933	27.533188544833447	28.596205738436836	21.70848075778678
32-33	21.917370455711584	27.656904020277466	28.571181993066997	21.85454353094395
34-35	22.07703915546628	27.73016431853259	28.531133569099453	21.661662956901676
36-37	22.02844559028091	27.6881905437362	28.475575974414706	21.807787891568182
38-39	21.864350670969134	27.729638779530585	28.54774330357101	21.85826724592927
40-41	22.200879151412515	27.561112240795094	28.498459027623685	21.739549580168703
42-43	21.988842769253296	27.642692266588636	28.32217426110318	22.04629070305489
44-45	22.08745139311728	27.739294228670946	28.162130024279357	22.011124353932416
46-47	22.10788207630148	27.74335279146446	28.034311296744303	22.11445383548976
48-49	21.989965287186003	27.96954087742523	27.97362182812663	22.06687200726214
50-51	22.04881310931712	28.056275230660887	27.749092415901295	22.145819244120695
52-53	22.09961648527931	27.848364685601457	27.66713737272356	22.38488145639567
54-55	22.21112329263101	27.611995065496274	27.95638395161098	22.220497690261734
56-57	22.079379861167205	27.447886228692433	28.037772519679592	22.434961390460774
58-59	21.952118172869618	27.288179078096913	28.059454158696784	22.700248590336685
60-61	22.08823256166878	27.49041423881804	28.297222870801225	22.124130328711956
62-63	21.72848097719805	27.688837395207244	28.294680456655545	22.288001170939157
64-65	21.601643071046208	27.928992963862974	28.003089200495463	22.466274764595358
66-67	21.659068181242404	28.39624512416141	27.594426935904398	22.35025975869179
68-69	21.745289318608265	28.434274150554938	27.427717064527158	22.39271946630964
70-71	21.725930432434065	27.983176418424723	27.464682014038182	22.82621113510303
72-73	21.900157831804385	27.583382790562826	27.4439256438387	23.07253373379409
74-75	21.956716538537147	27.643507357256723	27.573522275408063	22.82625382879807
76-77	22.221687937605815	27.564997148344904	27.097897041502232	23.115417872547045
78-79	22.378063772948607	27.602573194241675	26.73185877995418	23.287504252855534
80-81	22.214657108178976	27.928529142047054	26.899850409798404	22.956963339975573
82-83	22.227029041278676	27.740831422922525	26.930001882269757	23.102137653529052
84-85	22.260634294740473	28.053401831153497	26.938309306500376	22.747654567605654
86-87	22.619064228262474	27.944421066528047	26.985943996513566	22.45057070869591
88-89	22.37960793697088	28.08983407532601	27.22946620101015	22.301091786692957
90-91	22.39593392575489	27.799119508682953	27.109965076747898	22.694981488814264
92-93	22.339672045277876	27.456404214910986	27.35642497490166	22.847498764909474
94-95	21.72853674460764	27.856853000169686	27.844044970028015	22.570565285194657
96-97	21.78879510351719	27.82059484972452	28.033683071611176	22.356926975147108
98-99	22.933113645164095	29.729429019097353	28.370404813886474	18.967052521852075
100	22.03772662281846	30.947932845798952	21.959012928743412	25.05532760263917
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2307.0
1	1892.0
2	2095.5
3	2881.0
4	3662.5
5	5466.5
6	7133.0
7	8555.0
8	9799.0
9	10182.5
10	9653.5
11	8874.0
12	9369.5
13	11782.5
14	17613.0
15	26382.5
16	34290.0
17	39325.5
18	41004.0
19	39736.5
20	36743.5
21	33869.5
22	32562.0
23	33903.5
24	38872.5
25	47262.0
26	59166.5
27	73526.5
28	89597.5
29	107519.0
30	125482.5
31	144225.5
32	164284.5
33	183878.0
34	202700.5
35	221049.5
36	240078.5
37	258062.5
38	273454.5
39	286964.5
40	301336.5
41	314243.0
42	322652.0
43	329823.5
44	335777.0
45	340777.0
46	342390.5
47	341638.5
48	341569.0
49	337379.5
50	329703.5
51	318060.0
52	301446.0
53	282186.0
54	261496.0
55	240455.5
56	219074.0
57	196159.0
58	172065.5
59	147792.5
60	122492.5
61	99097.5
62	78997.5
63	61065.5
64	45682.5
65	34065.0
66	25869.5
67	19865.0
68	15070.0
69	10848.5
70	7832.0
71	5849.5
72	4140.0
73	2812.0
74	1961.5
75	1376.5
76	955.0
77	637.0
78	397.5
79	256.0
80	174.0
81	122.0
82	93.0
83	70.0
84	47.5
85	37.0
86	28.5
87	21.0
88	13.5
89	7.0
90	4.5
91	5.0
92	5.0
93	3.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	1.8537291531164215E-5
14-15	0.0
16-17	9.330436737352654E-4
18-19	0.0016003861688571772
20-21	0.0
22-23	0.0035163606248443053
24-25	2.287079822485518E-4
26-27	0.0017067190126457369
28-29	0.005457291312437724
30-31	0.0016168572420987254
32-33	0.0058918113197402805
34-35	0.01080868784397435
36-37	7.126005212110233E-4
38-39	3.13902122304805E-5
40-41	0.0029652010939447
42-43	0.005689225185310954
44-45	0.0019777432835454065
46-47	0.0021109232726514975
48-49	0.0029548594453416414
50-51	0.010484455270586153
52-53	0.006557007458086286
54-55	0.009795055427037081
56-57	0.018269344771830134
58-59	0.01206654919953711
60-61	0.01583826195620887
62-63	0.014601098198248605
64-65	0.010440326510744285
66-67	0.012665564807924667
68-69	0.008552527045048323
70-71	0.009094349719336722
72-73	0.006350639361808305
74-75	0.01049489610688053
76-77	0.012180205499298677
78-79	0.006831429725477918
80-81	0.004220690536935764
82-83	0.0021582502492632216
84-85	9.643809601525205E-5
86-87	0.00418534696526342
88-89	6.148863063832714E-4
90-91	6.001844874778432E-4
92-93	0.0015392003065274437
94-95	0.0010023575289977657
96-97	4.325899913694216E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	171.0
20-21	585.0
22-23	1401.0
24-25	3034.0
26-27	4168.0
28-29	7292.0
30-31	16783.0
32-33	21374.0
34-35	28802.0
36-37	40898.0
38-39	29554.0
40-41	49882.0
42-43	59399.0
44-45	66459.0
46-47	64703.0
48-49	58157.0
50-51	33583.0
52-53	11853.0
54-55	12372.0
56-57	11991.0
58-59	34788.0
60-61	58324.0
62-63	57473.0
64-65	60202.0
66-67	63901.0
68-69	64965.0
70-71	64971.0
72-73	64240.0
74-75	65433.0
76-77	64582.0
78-79	69857.0
80-81	71918.0
82-83	70601.0
84-85	72875.0
86-87	77847.0
88-89	86210.0
90-91	95394.0
92-93	108925.0
94-95	137051.0
96-97	339020.0
98-99	621845.0
100-101	5218914.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.8884889925638
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.22856307849166	77.49594946781879
2	6.834333009710036	11.739810709519915
3	1.6836654448780137	4.338224430886959
4	0.5863881299407266	2.0145596177513667
5	0.2542102805896529	1.0916868443110477
6	0.13825721618391304	0.7124822034212717
7	0.07520478710131644	0.45214578703976643
8	0.05638410209610063	0.38741962657892254
9	0.03451246350065967	0.26678010073344005
>10	0.10693523632232349	1.3545678831048953
>50	0.0011417734419434692	0.06530080735392092
>100	3.603893943104001E-4	0.04975667409054643
>500	4.4088349383150675E-5	0.03131584738909248
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.471638870821895E-5	0.0	0.0	0.0
3	0.0	2.471638870821895E-5	0.0	1.2358194354109476E-5	0.0
4	0.0	6.179097177054739E-5	0.0	1.2358194354109476E-5	0.0
5	0.0	6.179097177054739E-5	0.0	2.471638870821895E-5	0.0
6	1.2358194354109476E-5	6.179097177054739E-5	0.0	2.471638870821895E-5	0.0
7	1.2358194354109476E-5	6.179097177054739E-5	0.0	4.94327774164379E-5	0.0
8	1.2358194354109476E-5	7.414916612465686E-5	0.0	4.94327774164379E-5	0.0
9	1.2358194354109476E-5	8.650736047876633E-5	0.0	1.2358194354109478E-4	0.0
10-11	1.2358194354109476E-5	8.650736047876633E-5	0.0	1.2358194354109478E-4	0.0
12-13	1.2358194354109476E-5	8.650736047876633E-5	0.0	1.2358194354109478E-4	0.0
14-15	1.8537291531164215E-5	1.297610407181495E-4	0.0	1.297610407181495E-4	1.2358194354109476E-5
16-17	3.707458306232843E-5	1.4829833224931372E-4	0.0	1.3594013789520424E-4	1.2358194354109476E-5
18-19	3.707458306232843E-5	1.6683562378047792E-4	0.0	1.5447742942636847E-4	1.8537291531164215E-5
20-21	3.707458306232843E-5	1.8537291531164215E-4	0.0	1.8537291531164215E-4	3.707458306232843E-5
22-23	3.707458306232843E-5	1.9155201248869687E-4	0.0	2.100893040198611E-4	6.179097177054738E-5
24-25	4.325368023938317E-5	2.100893040198611E-4	0.0	2.3480569272808007E-4	8.650736047876633E-5
26-27	4.94327774164379E-5	2.2244749837397058E-4	0.0	3.336712475609559E-4	8.650736047876633E-5
28-29	4.94327774164379E-5	2.2244749837397058E-4	0.0	8.032826330171159E-4	8.650736047876633E-5
30-31	4.94327774164379E-5	2.4716388708218956E-4	0.0	0.0026261163002482637	8.650736047876633E-5
32-33	4.94327774164379E-5	2.4716388708218956E-4	0.0	0.006902051546770142	8.650736047876633E-5
34-35	6.179097177054739E-5	2.8423847014451796E-4	0.0	0.012166642341620779	8.650736047876633E-5
36-37	6.179097177054739E-5	3.2131305320684636E-4	0.0	0.02343731559256862	9.88655548328758E-5
38-39	6.179097177054739E-5	3.2131305320684636E-4	0.0	0.15247540194100273	1.3594013789520424E-4
40-41	6.179097177054739E-5	3.4602944191506534E-4	0.0	0.2868213327645268	1.3594013789520424E-4
42-43	6.179097177054739E-5	3.583876362691748E-4	0.0	0.31076533432561393	1.3594013789520424E-4
44-45	6.179097177054739E-5	4.0164131650855797E-4	0.0	0.3426309384676852	1.3594013789520424E-4
46-47	6.179097177054739E-5	4.325368023938317E-4	0.0	0.37609074968143663	1.3594013789520424E-4
48-49	6.179097177054739E-5	4.325368023938317E-4	0.0	0.4084383234033182	1.3594013789520424E-4
50-51	6.179097177054739E-5	4.5725319110205066E-4	0.0	0.43970455511921513	1.3594013789520424E-4
52-53	6.179097177054739E-5	4.5725319110205066E-4	0.0	0.8326148073165949	1.3594013789520424E-4
54-55	6.179097177054739E-5	4.634322882791054E-4	0.0	1.3609894069265454	1.3594013789520424E-4
56-57	7.414916612465686E-5	4.8196957981026957E-4	0.0	1.9345641023866516	1.4211923507225898E-4
58-59	7.414916612465686E-5	4.8196957981026957E-4	0.0	2.5288696688757764	1.4829833224931372E-4
60-61	7.414916612465686E-5	4.8196957981026957E-4	0.0	2.6887847038179533	1.5447742942636847E-4
62-63	7.414916612465686E-5	4.943277741643791E-4	0.0	2.6958597700856806	1.6065652660342318E-4
64-65	8.03282633017116E-5	6.426261064136927E-4	0.0	2.7017113751123514	1.6065652660342318E-4
66-67	8.650736047876633E-5	6.735215922989664E-4	0.0	2.7068832794495465	1.6065652660342318E-4
68-69	8.650736047876633E-5	6.858797866530759E-4	0.0	2.711919243648846	1.6683562378047792E-4
70-71	8.650736047876633E-5	6.982379810071854E-4	0.0	2.715898582230869	1.8537291531164215E-4
72-73	9.88655548328758E-5	7.105961753612949E-4	0.0	2.71883365338997	1.977311096657516E-4
74-75	9.88655548328758E-5	7.353125640695139E-4	0.0	2.721404157815625	1.977311096657516E-4
76-77	1.1122374918698529E-4	7.723871471318423E-4	0.0	2.7237460356457284	1.977311096657516E-4
78-79	1.1122374918698529E-4	8.032826330171159E-4	0.0	2.725327884523055	2.0391020684280636E-4
80-81	1.1122374918698529E-4	8.156408273712254E-4	0.0	2.725865465977458	2.1626840119691584E-4
82-83	1.1740284636404004E-4	8.218199245482802E-4	0.0	2.7263165400713834	2.5334298425924425E-4
84-85	1.2358194354109478E-4	8.712527019647181E-4	0.0	2.7264277638205705	2.59522081436299E-4
86-87	1.2358194354109478E-4	8.897899934958823E-4	0.0	2.726538987569757	2.59522081436299E-4
88	1.2358194354109478E-4	9.268645765582107E-4	0.0	2.72657606215282	2.59522081436299E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9590	0.0	50.532127	1
ATCTCGT	6330	0.0	41.485924	94
GTATCAA	16810	0.0	36.818985	1
TATCTCG	7150	0.0	32.125343	94
TATGCCG	3375	0.0	30.721592	86-87
TCAACGC	19670	0.0	30.675056	4
ATCAACG	19880	0.0	30.394478	3
CAACGCA	20325	0.0	29.686512	5
AACGCAG	20845	0.0	29.008112	6
TATCAAC	21045	0.0	28.855574	2
GCCGTCT	3875	0.0	28.462759	90-91
ATGCCGT	3760	0.0	28.447184	88-89
CCGTCTT	3785	0.0	28.384638	90-91
TGCCGTC	3770	0.0	28.223957	88-89
CTCGTAT	3620	0.0	27.894526	82-83
CGTATGC	3710	0.0	27.737976	84-85
TCGTATG	3840	0.0	27.149702	84-85
TCTCGTA	3945	0.0	26.000654	82-83
ACGCAGA	24325	0.0	24.9114	7
CGCAGAG	24565	0.0	24.650434	8
>>END_MODULE