FastQCFastQC Report
Fri 27 May 2016
SRR2139375_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139375_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10184256
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111500.049.9888231
ATCTCGT84350.042.9860694
GTATCAA200100.036.767531
TATCTCG102600.034.08484394
TATGCCG46250.031.92436286-87
GCCGTCT50300.031.7618390-91
CCGTCTT49300.031.2361990-91
ATGCCGT49900.030.86821288-89
TGCCGTC49000.030.57472688-89
TCAACGC238350.029.9656454
ATCAACG239500.029.821763
TCGTATG51550.029.2571784-85
CTCGTAT50100.029.14984582-83
TCTCGTA51400.029.09032482-83
CAACGCA246500.028.9923275
TATCAAC254050.028.4182822
CGTATGC52250.028.24109584-85
AACGCAG253750.028.1978456
CGCTATC161150.025.19158294
ACGCAGA291100.024.565127
Produced by FastQC (version 0.11.5)