##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139375_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10184256
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06928517900571	33.0	31.0	34.0	30.0	34.0
2	32.2511879120085	33.0	31.0	34.0	30.0	34.0
3	32.27198550389935	34.0	31.0	34.0	30.0	34.0
4	36.00963830838502	37.0	35.0	37.0	35.0	37.0
5	35.87881196230731	37.0	35.0	37.0	35.0	37.0
6	35.898603098743784	37.0	35.0	37.0	35.0	37.0
7	35.840339834348235	37.0	35.0	37.0	35.0	37.0
8	35.82754449613207	37.0	35.0	37.0	35.0	37.0
9	37.60628729285674	39.0	37.0	39.0	35.0	39.0
10-11	37.486208614551714	39.0	37.0	39.0	34.5	39.0
12-13	37.46836784149966	39.0	37.0	39.0	34.0	39.0
14-15	38.728966701151265	40.0	38.0	41.0	34.0	41.0
16-17	38.68726777881467	40.0	38.0	41.0	34.0	41.0
18-19	38.702022317585104	40.0	38.0	41.0	34.0	41.0
20-21	38.68327545095636	40.0	38.0	41.0	34.0	41.0
22-23	38.60421744990106	40.0	38.0	41.0	34.0	41.0
24-25	38.550177818589965	40.0	38.0	41.0	34.0	41.0
26-27	38.38657518569845	40.0	38.0	41.0	34.0	41.0
28-29	38.28151643022818	40.0	38.0	41.0	34.0	41.0
30-31	38.13615694252576	40.0	38.0	41.0	33.0	41.0
32-33	37.968658968365666	40.0	37.0	41.0	33.0	41.0
34-35	38.0534118788879	40.0	37.0	41.0	33.0	41.0
36-37	38.0294275762171	40.0	37.0	41.0	33.0	41.0
38-39	37.997409370673964	40.0	37.0	41.0	33.0	41.0
40-41	37.92756412478728	40.0	37.0	41.0	33.0	41.0
42-43	37.82372931611041	40.0	37.0	41.0	33.0	41.0
44-45	37.88702845322179	40.0	37.0	41.0	33.0	41.0
46-47	37.88706796930023	40.0	37.0	41.0	33.0	41.0
48-49	37.836356002108644	40.0	37.0	41.0	33.0	41.0
50-51	37.74732376561132	40.0	37.0	41.0	32.5	41.0
52-53	37.56401213729271	40.0	36.5	41.0	32.0	41.0
54-55	37.44468752971498	39.0	36.0	41.0	32.0	41.0
56-57	37.28612680344531	39.0	36.0	41.0	31.0	41.0
58-59	37.159043610924115	39.0	35.5	41.0	31.0	41.0
60-61	36.94326758740219	39.0	35.0	40.5	31.0	41.0
62-63	36.740695288664654	38.5	35.0	40.0	31.0	41.0
64-65	36.45120840228499	38.0	35.0	40.0	30.5	41.0
66-67	36.175779634645394	37.5	35.0	40.0	30.0	41.0
68-69	35.871324715628084	37.0	34.0	40.0	30.0	41.0
70-71	35.57777772106519	36.5	34.0	39.0	30.0	41.0
72-73	35.28551612595156	36.0	34.0	39.0	29.0	40.5
74-75	34.901496396956716	35.5	34.0	38.5	29.0	40.0
76-77	33.59888976410343	34.5	32.0	36.5	27.5	39.0
78-79	34.241746176579994	35.0	33.0	37.0	29.0	39.0
80-81	34.14189290532305	35.0	33.0	36.5	29.0	39.0
82-83	33.85385621630387	35.0	33.0	36.0	29.0	38.0
84-85	33.688732652288955	35.0	33.0	36.0	29.0	37.0
86-87	33.451212172595106	35.0	33.0	35.0	29.0	37.0
88-89	33.352004676440544	35.0	33.0	35.0	29.0	36.5
90-91	33.23352885508221	35.0	33.0	35.0	29.0	36.0
92-93	33.11821277082261	35.0	33.0	35.0	29.0	36.0
94-95	33.06525185227481	35.0	33.0	35.0	29.0	36.0
96-97	33.11500178084486	35.0	33.0	35.0	29.0	35.0
98-99	33.24830075243013	35.0	33.0	35.0	29.0	35.0
100	32.108643975165904	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	8.0
20	48.0
21	296.0
22	1533.0
23	5315.0
24	14721.0
25	31537.0
26	58778.0
27	93941.0
28	135363.0
29	181734.0
30	230605.0
31	284581.0
32	351135.0
33	439222.0
34	575088.0
35	786462.0
36	1193433.0
37	2027409.0
38	2574835.0
39	1153620.0
40	44592.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.044922672800055	20.46286935442314	14.727732688573422	23.76447528420338
2	14.540708717455649	21.48322862268977	41.365780671656324	22.610281988198256
3	17.844396291687875	26.489720996801335	30.952167738124416	24.713714973386374
4	12.546630799539995	18.616391811046384	39.26319212714213	29.57378526227149
5	13.08339067674654	38.07859896687593	35.83246532687316	13.005545029504365
6	29.435787945629016	38.4675326307587	19.105214951391638	12.99146447222065
7	26.16755706062377	32.91166286471982	23.493360732487478	17.427419342168932
8	24.256342338605783	36.8104552752798	21.368188309484758	17.565014076629655
9	25.55024147075643	17.0308758931433	21.285452761595938	36.13342987450433
10-11	23.92956834549328	26.836962857178765	29.314379960598004	19.919088836729948
12-13	24.92155292655182	24.415831103993373	29.202494438719377	21.460121530735435
14-15	22.007680286120067	25.856385581823556	26.785879105945494	25.35005502611089
16-17	21.643329726417743	28.945610527742154	27.43340625718028	21.97765348865982
18-19	21.68180129051187	28.245131368279676	28.979727562532904	21.09333977867555
20-21	22.49938493660023	27.42159089296699	28.85158199805291	21.22744217237987
22-23	22.20682194934163	27.33405219823387	28.669373212658307	21.789752639766192
24-25	22.02308680232804	27.46795345433015	28.68636382544112	21.822595917900692
26-27	21.96298952232196	27.63491513859007	28.601407046275824	21.80068829281215
28-29	21.90057936027366	27.703331003254522	28.62409641030048	21.77199322617134
30-31	22.185885666546287	27.492566169105793	28.557699231860855	21.76384893248707
32-33	21.985916176797986	27.6563681744043	28.488335717545116	21.869379931252595
34-35	22.130619176680426	27.69081519345856	28.475666404772387	21.702899225088625
36-37	22.090567463858367	27.627211153415693	28.431765432343653	21.85045595038229
38-39	21.892438629311872	27.710798056214415	28.50171223125941	21.895051083214298
40-41	22.281927684945334	27.5221932190938	28.436268881399883	21.759610214560983
42-43	22.032742569528192	27.626644194133714	28.240142522956557	22.100470713381533
44-45	22.104307737166522	27.68572844945397	28.122035906260646	22.087927907118864
46-47	22.14155047308948	27.69173420490744	28.040096116171437	22.126619205831638
48-49	22.04140597989415	27.941442298742054	27.93734915627244	22.079802565091352
50-51	22.077527351038256	28.003031856116916	27.715058939636915	22.204381853207913
52-53	22.145266595095727	27.799826048789335	27.612695601875075	22.442211754239864
54-55	22.24400802806741	27.55481419167292	27.943081547086933	22.258096233172736
56-57	22.11195347830231	27.32536435833632	28.04422022924375	22.51846193411762
58-59	21.95014869705083	27.188252603220658	28.040631109697085	22.820967590031426
60-61	22.099048460826694	27.421139743572038	28.332182463505916	22.14762933209535
62-63	21.709309436249665	27.618779247870467	28.31627981415482	22.355631501725046
64-65	21.568122808763935	27.87564735495584	27.990757886073304	22.565471950206916
66-67	21.633338538085845	28.429404617187725	27.546613191240866	22.39064365348556
68-69	21.721351477301425	28.468438331654323	27.357913484017104	22.45229670702715
70-71	21.718798890974902	27.985566273049873	27.356689440971703	22.938945395003522
72-73	21.881492400359388	27.54046888070871	27.380958858519445	23.197079860412465
74-75	21.96820290262521	27.582279503322987	27.547137048099458	22.90238054595234
76-77	22.257857942227922	27.48816391142361	26.99276810898387	23.2612100373646
78-79	22.43930067294185	27.51530461142597	26.60501836645813	23.44037634917405
80-81	22.275354324063315	27.89448001175118	26.749510927224744	23.08065473696076
82-83	22.247178801501487	27.693208098971684	26.81731366624696	23.242299433279868
84-85	22.31465540740702	28.024054509829472	26.806006533493214	22.855283549270297
86-87	22.725446356966803	27.91806125687196	26.875503950926937	22.4809884352343
88-89	22.451907799918043	28.07988115687774	27.16483267466402	22.303378368540198
90-91	22.44441886791165	27.73121642369133	27.01198501849421	22.81237968990281
92-93	22.388713662940496	27.35643391042228	27.276033155997826	22.978819270639402
94-95	21.73511400298419	27.77697872861204	27.83422774759013	22.65367952081364
96-97	21.782919659035397	27.699261373417826	28.065714223195094	22.452104744351683
98-99	22.944005344234796	29.626854779858913	28.361353049880734	19.06778682602555
100	22.024415410626126	30.829030918965955	21.935162167409644	25.211391502998275
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2502.0
1	2135.5
2	2508.0
3	3428.0
4	4330.5
5	6514.0
6	8523.0
7	10109.5
8	11354.5
9	11660.5
10	11156.5
11	10316.5
12	10826.0
13	13841.0
14	21036.5
15	31766.0
16	41331.5
17	47407.5
18	49948.0
19	48942.0
20	45894.0
21	42493.0
22	40434.0
23	42351.0
24	49210.0
25	60367.0
26	76134.0
27	94758.0
28	114927.5
29	137738.5
30	160945.0
31	184878.5
32	210422.5
33	234515.5
34	258592.5
35	281765.0
36	305510.5
37	329040.5
38	348258.5
39	364767.0
40	382730.0
41	398792.0
42	409182.0
43	417832.0
44	423710.5
45	428868.5
46	431814.0
47	430836.0
48	429694.5
49	424089.0
50	414507.5
51	400807.0
52	381756.5
53	359217.0
54	334969.0
55	309264.0
56	281338.5
57	252206.0
58	221583.0
59	190449.5
60	158437.0
61	128359.5
62	102024.0
63	78586.0
64	58538.0
65	43436.5
66	32872.0
67	25333.5
68	19285.0
69	14077.0
70	10242.0
71	7436.0
72	5293.0
73	3694.0
74	2466.5
75	1626.5
76	1072.0
77	675.5
78	458.0
79	324.5
80	222.5
81	141.0
82	98.5
83	69.5
84	50.5
85	39.5
86	32.0
87	28.0
88	19.0
89	9.5
90	3.5
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	9.81907760370517E-6
14-15	0.0
16-17	0.0011390130020297999
18-19	0.0015465047225835644
20-21	0.0
22-23	0.003358518292435026
24-25	2.308213261225406E-4
26-27	0.001999554399056525
28-29	0.005451684068960018
30-31	0.001914687622898089
32-33	0.0058911033977456905
34-35	0.011369129808190357
36-37	6.562146585528707E-4
38-39	1.4985392988184766E-5
40-41	0.002551254860818501
42-43	0.00583628679452619
44-45	0.0017394658635945556
46-47	0.00196676962516872
48-49	0.0031684489798165646
50-51	0.010786496332879236
52-53	0.006665339201423031
54-55	0.009783979722123404
56-57	0.018148168283513056
58-59	0.011970308570181724
60-61	0.01672907763301077
62-63	0.013993794745466637
64-65	0.01093084871829588
66-67	0.012468051299709883
68-69	0.008268748975690153
70-71	0.008982909639428674
72-73	0.006607483137316008
74-75	0.010686015584593858
76-77	0.011872714888418784
78-79	0.0064762861913041795
80-81	0.004088215514369056
82-83	0.0021969076516876146
84-85	4.1799434608904784E-5
86-87	0.0038843528480884577
88-89	5.811934250260788E-4
90-91	6.762913473051404E-4
92-93	0.001569539289161329
94-95	0.001348377285724229
96-97	5.338583159801919E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	212.0
20-21	656.0
22-23	1570.0
24-25	3482.0
26-27	5119.0
28-29	9538.0
30-31	23111.0
32-33	29731.0
34-35	40096.0
36-37	57239.0
38-39	40534.0
40-41	68388.0
42-43	80472.0
44-45	90425.0
46-47	87219.0
48-49	79162.0
50-51	44382.0
52-53	14046.0
54-55	14117.0
56-57	13653.0
58-59	46304.0
60-61	79479.0
62-63	77211.0
64-65	81244.0
66-67	86597.0
68-69	87775.0
70-71	87161.0
72-73	84720.0
74-75	87036.0
76-77	85886.0
78-79	92924.0
80-81	95118.0
82-83	92769.0
84-85	94987.0
86-87	101874.0
88-89	111657.0
90-91	122380.0
92-93	139172.0
94-95	172830.0
96-97	427404.0
98-99	773029.0
100-101	6453547.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.80601524330964
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.51630673054477	75.9152127311536
2	7.387461605875449	12.53002363114476
3	1.7308364760255763	4.403560337085041
4	0.6136572992813648	2.0816732110809455
5	0.27960617978444985	1.1856142972461814
6	0.15685347370239391	0.7981270849062772
7	0.09075702178077086	0.5387718960804208
8	0.05620235332938167	0.38130381065291324
9	0.03745413208853821	0.2858702127142954
>10	0.12816954289722132	1.6580636675039198
>50	0.002264319351380894	0.1291479433769762
>100	3.9318818592808124E-4	0.062244394131480495
>500	1.883866737510769E-5	0.0113751420655484
>1k	1.8838485385905146E-5	0.01901164085769546
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.81907760370517E-6
2	0.0	2.945723281111551E-5	0.0	0.0	9.81907760370517E-6
3	0.0	2.945723281111551E-5	0.0	0.0	9.81907760370517E-6
4	0.0	3.927631041482068E-5	0.0	9.81907760370517E-6	9.81907760370517E-6
5	0.0	3.927631041482068E-5	0.0	9.81907760370517E-6	9.81907760370517E-6
6	0.0	3.927631041482068E-5	0.0	9.81907760370517E-6	1.963815520741034E-5
7	0.0	3.927631041482068E-5	0.0	9.81907760370517E-6	1.963815520741034E-5
8	0.0	3.927631041482068E-5	0.0	9.81907760370517E-6	3.927631041482068E-5
9	0.0	8.837169843334653E-5	0.0	2.945723281111551E-5	5.891446562223102E-5
10-11	0.0	8.837169843334653E-5	0.0	3.4366771612968096E-5	5.891446562223102E-5
12-13	0.0	8.837169843334653E-5	0.0	3.927631041482068E-5	5.891446562223102E-5
14-15	1.4728616405557754E-5	1.5710524165928272E-4	0.0	3.927631041482068E-5	6.873354322593619E-5
16-17	4.4185849216673264E-5	1.5710524165928272E-4	0.0	3.927631041482068E-5	1.1291939244260945E-4
18-19	4.909538801852585E-5	1.7674339686669306E-4	0.0	3.927631041482068E-5	1.3746708645187238E-4
20-21	4.909538801852585E-5	2.0620062967780856E-4	0.0	4.4185849216673264E-5	1.3746708645187238E-4
22-23	4.909538801852585E-5	2.7493417290374477E-4	0.0	6.382400442408361E-5	1.4237662525372498E-4
24-25	5.891446562223102E-5	3.682154101389439E-4	0.0	1.0800985364075687E-4	1.5710524165928272E-4
26-27	5.891446562223102E-5	3.8294402654450165E-4	0.0	2.1601970728151373E-4	1.5710524165928272E-4
28-29	5.891446562223102E-5	3.8294402654450165E-4	0.0	8.002548247019713E-4	1.6201478046113532E-4
30-31	7.364308202778878E-5	4.3203941456302746E-4	0.0	0.002862261121480057	1.669243192629879E-4
32-33	7.855262082964136E-5	4.3203941456302746E-4	0.0	0.007609785142871507	1.8656247447039823E-4
34-35	8.837169843334653E-5	5.007729577889636E-4	0.0	0.013324488308227916	1.963815520741034E-4
36-37	9.81907760370517E-5	5.940541950241627E-4	0.0	0.0253577679115686	1.963815520741034E-4
38-39	9.81907760370517E-5	6.676972770519516E-4	0.0	0.17498087243682797	1.963815520741034E-4
40-41	9.81907760370517E-5	7.462498978815929E-4	0.0	0.3298129976308529	1.963815520741034E-4
42-43	9.81907760370517E-5	7.560689754852982E-4	0.0	0.35496456491274375	1.963815520741034E-4
44-45	9.81907760370517E-5	8.149834411075291E-4	0.0	0.38903185465879886	1.963815520741034E-4
46-47	9.81907760370517E-5	8.640788291260549E-4	0.0	0.4263541686304822	1.963815520741034E-4
48-49	9.81907760370517E-5	8.738979067297601E-4	0.0	0.4602889008288873	2.1601970728151373E-4
50-51	9.81907760370517E-5	9.573600663612541E-4	0.0	0.49344792589659964	2.1601970728151373E-4
52-53	1.0800985364075687E-4	9.622696051631067E-4	0.0	0.9370296661827825	2.258387848852189E-4
54-55	1.0800985364075687E-4	9.720886827668118E-4	0.0	1.5282706954734837	2.3565786248892407E-4
56-57	1.0800985364075687E-4	9.81907760370517E-4	0.0	2.173521561123365	2.4547694009262924E-4
58-59	1.0800985364075687E-4	9.868172991723697E-4	0.0	2.843325030321312	2.5529601769633443E-4
60-61	1.0800985364075687E-4	0.0010113649931816327	0.0	3.023495285271698	2.5529601769633443E-4
62-63	1.1291939244260945E-4	0.0010211840707853377	0.0	3.030501197141941	2.5529601769633443E-4
64-65	1.1782893124446203E-4	0.0010702794588038635	0.0	3.036731401881492	2.5529601769633443E-4
66-67	1.1782893124446203E-4	0.0010800985364075688	0.0	3.0418176840802116	2.60205556498187E-4
68-69	1.1782893124446203E-4	0.0010997366916149789	0.0	3.0469677902833547	2.700246341018922E-4
70-71	1.1782893124446203E-4	0.001109555769218684	0.0	3.0514992945974653	2.847532505074499E-4
72-73	1.1782893124446203E-4	0.0011193748468223894	0.0	3.0547739569783006	3.0439140571486025E-4
74-75	1.1782893124446203E-4	0.0011537416184353574	0.0	3.0577049516430064	3.0439140571486025E-4
76-77	1.1782893124446203E-4	0.0012224751616612935	0.0	3.0605426650704777	3.0930094451671287E-4
78-79	1.1782893124446203E-4	0.0012470228556705565	0.0	3.062349375349559	3.1421048331856544E-4
80-81	1.1782893124446203E-4	0.0012470228556705565	0.0	3.063026891704215	3.289390997241232E-4
82-83	1.1782893124446203E-4	0.0012470228556705565	0.0	3.0634049261919576	3.927631041482068E-4
84-85	1.1782893124446203E-4	0.0012470228556705565	0.0	3.0636013077440314	3.927631041482068E-4
86-87	1.1782893124446203E-4	0.0012813896272835246	0.0	3.0636700412872577	3.927631041482068E-4
88	1.2764800884816722E-4	0.0013648517869150186	0.0	3.0636700412872577	3.927631041482068E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11150	0.0	49.988823	1
ATCTCGT	8435	0.0	42.98606	94
GTATCAA	20010	0.0	36.76753	1
TATCTCG	10260	0.0	34.084843	94
TATGCCG	4625	0.0	31.924362	86-87
GCCGTCT	5030	0.0	31.76183	90-91
CCGTCTT	4930	0.0	31.23619	90-91
ATGCCGT	4990	0.0	30.868212	88-89
TGCCGTC	4900	0.0	30.574726	88-89
TCAACGC	23835	0.0	29.965645	4
ATCAACG	23950	0.0	29.82176	3
TCGTATG	5155	0.0	29.25717	84-85
CTCGTAT	5010	0.0	29.149845	82-83
TCTCGTA	5140	0.0	29.090324	82-83
CAACGCA	24650	0.0	28.992327	5
TATCAAC	25405	0.0	28.418282	2
CGTATGC	5225	0.0	28.241095	84-85
AACGCAG	25375	0.0	28.197845	6
CGCTATC	16115	0.0	25.191582	94
ACGCAGA	29110	0.0	24.56512	7
>>END_MODULE