FastQCFastQC Report
Fri 27 May 2016
SRR2139374_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139374_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7832771
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94400.049.4102821
ATCTCGT64500.042.3375494
GTATCAA165900.036.7781521
TATGCCG34700.034.71450486-87
CCGTCTT36350.034.61516690-91
GCCGTCT36950.034.36406790-91
TATCTCG76850.031.6733294
ATGCCGT39800.031.37817888-89
TGCCGTC39400.031.12818588-89
TCAACGC196050.030.3100664
CTCGTAT38700.030.15334982-83
ATCAACG198400.030.0594923
CGTATGC40100.029.84645584-85
CAACGCA201300.029.5409415
TCTCGTA40250.029.4565782-83
TCGTATG41650.029.25582784-85
AACGCAG207600.028.7273736
TATCAAC212400.028.2807522
ACGCAGA237200.025.0880897
CGCAGAG242200.024.5879338
Produced by FastQC (version 0.11.5)