##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139374_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7832771
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09296875907645	33.0	31.0	34.0	30.0	34.0
2	32.273262680601796	33.0	31.0	34.0	30.0	34.0
3	32.29181396979434	34.0	31.0	34.0	30.0	34.0
4	36.0209372902642	37.0	35.0	37.0	35.0	37.0
5	35.892744598303715	37.0	35.0	37.0	35.0	37.0
6	35.91408161939115	37.0	35.0	37.0	35.0	37.0
7	35.862430677470336	37.0	35.0	37.0	35.0	37.0
8	35.8522484571552	37.0	35.0	37.0	35.0	37.0
9	37.632079885905	39.0	37.0	39.0	35.0	39.0
10-11	37.51714616704612	39.0	37.0	39.0	35.0	39.0
12-13	37.493449827653585	39.0	37.0	39.0	35.0	39.0
14-15	38.757649878950886	40.0	38.0	41.0	34.5	41.0
16-17	38.71843291473733	40.0	38.0	41.0	34.0	41.0
18-19	38.736821617790184	40.0	38.0	41.0	34.0	41.0
20-21	38.72153490945072	40.0	38.0	41.0	34.0	41.0
22-23	38.64164080651524	40.0	38.0	41.0	34.0	41.0
24-25	38.58183882065035	40.0	38.0	41.0	34.0	41.0
26-27	38.41504128075721	40.0	38.0	41.0	34.0	41.0
28-29	38.3119295647994	40.0	38.0	41.0	34.0	41.0
30-31	38.16583636654074	40.0	38.0	41.0	33.5	41.0
32-33	37.99668502614743	40.0	37.0	41.0	33.0	41.0
34-35	38.08388799802617	40.0	37.5	41.0	33.0	41.0
36-37	38.05843366594445	40.0	37.0	41.0	33.0	41.0
38-39	38.02552098453617	40.0	37.0	41.0	33.0	41.0
40-41	37.95636237027844	40.0	37.0	41.0	33.0	41.0
42-43	37.85560460696795	40.0	37.0	41.0	33.0	41.0
44-45	37.913104810893444	40.0	37.0	41.0	33.0	41.0
46-47	37.91301768368379	40.0	37.0	41.0	33.0	41.0
48-49	37.86271417554744	40.0	37.0	41.0	33.0	41.0
50-51	37.774412221335695	40.0	37.0	41.0	32.5	41.0
52-53	37.597198666537714	40.0	36.5	41.0	32.0	41.0
54-55	37.47944987247658	39.5	36.0	41.0	32.0	41.0
56-57	37.32069102893216	39.0	36.0	41.0	31.0	41.0
58-59	37.19244733206651	39.0	35.5	41.0	31.0	41.0
60-61	36.98114821313937	39.0	35.0	41.0	31.0	41.0
62-63	36.78070444619887	38.5	35.0	40.0	31.0	41.0
64-65	36.4949465440307	38.0	35.0	40.0	31.0	41.0
66-67	36.22275977439524	37.5	35.0	40.0	30.0	41.0
68-69	35.91931606832527	37.0	34.0	40.0	30.0	41.0
70-71	35.627446658967216	36.5	34.0	39.0	30.0	41.0
72-73	35.340483480958426	36.0	34.0	39.0	29.5	40.5
74-75	34.959115833090635	36.0	34.0	39.0	29.0	40.0
76-77	33.6518678134575	34.5	32.0	36.5	28.0	39.0
78-79	34.297850877811086	35.0	33.5	37.0	29.0	39.0
80-81	34.19477346790265	35.0	34.0	37.0	29.0	39.0
82-83	33.90578046127301	35.0	33.0	36.0	29.0	38.5
84-85	33.73483024509045	35.0	33.0	36.0	29.0	37.0
86-87	33.497330208372155	35.0	33.0	35.5	29.0	37.0
88-89	33.39182459578096	35.0	33.0	35.0	29.0	36.5
90-91	33.26782077032341	35.0	33.0	35.0	29.0	36.0
92-93	33.154106858856494	35.0	33.0	35.0	29.0	36.0
94-95	33.09971278646326	35.0	33.0	35.0	29.0	36.0
96-97	33.14744772585567	35.0	33.0	35.0	29.0	35.0
98-99	33.277140309864436	35.0	33.0	35.0	29.0	35.0
100	32.10895355879132	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	36.0
21	233.0
22	1169.0
23	4197.0
24	11136.0
25	24484.0
26	45271.0
27	71509.0
28	103672.0
29	138329.0
30	174933.0
31	215512.0
32	264107.0
33	332028.0
34	435329.0
35	596425.0
36	910371.0
37	1559053.0
38	2007576.0
39	903507.0
40	33890.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.01114407659818	20.520017756168283	14.746747989951448	23.722090177282087
2	14.455650497122921	21.49521542248586	41.42895279333457	22.62018128705665
3	17.80422279675992	26.555672315710492	30.951396383221212	24.688708504308373
4	12.440693082945998	18.578801805899854	39.379154580160716	29.601350530993436
5	13.006597537448753	38.086304323208225	35.91089283728581	12.996205302057215
6	29.28644281825678	38.58422517395185	19.143621076117252	12.985710931674117
7	26.065207319350968	32.96700490796935	23.53578318579721	17.43200458688247
8	24.2034906931404	36.78961123719818	21.414988386613114	17.59190968304831
9	25.46435993085972	17.031201346241325	21.377926151549687	36.126512571349274
10-11	23.875247980567796	26.86763726400274	29.34128930872612	19.915825446703344
12-13	24.83554670499112	24.485913095123042	29.24556967132066	21.43297052856518
14-15	21.940384950637835	25.91643659162489	26.887312733087228	25.255865724650047
16-17	21.512873728279512	29.035596428100874	27.533335903755233	21.918193939864384
18-19	21.553378411805728	28.250547788021596	29.15253003857258	21.0435437616001
20-21	22.390696914911633	27.459622239314825	28.99434324819852	21.155337597575027
22-23	22.08773537919784	27.35905595050066	28.83444293863843	21.71876573166307
24-25	21.877841628931538	27.506265632151965	28.897942226427126	21.717950512489374
26-27	21.81206287228524	27.678409436464595	28.792760896729895	21.716766794520268
28-29	21.777860171353776	27.792738959571565	28.78802281520737	21.641378053867285
30-31	22.075466299997675	27.60886669724802	28.694178233720248	21.62148876903406
32-33	21.829064642810557	27.7539022486841	28.66242777911768	21.754605329387662
34-35	21.93840946561949	27.8007386116331	28.66461066217188	21.59624126057553
36-37	21.962903838431792	27.685600392807526	28.633573046246674	21.71792272251401
38-39	21.75947553913404	27.832289687471278	28.632580910292138	21.775653863102544
40-41	22.12570751418228	27.654599219277582	28.578865018027972	21.640828248512165
42-43	21.916498737614102	27.7178713007523	28.421263056627684	21.944366905005914
44-45	21.991185933303957	27.812979444344414	28.228966785936088	21.96686783641554
46-47	22.003411249816292	27.77497663683374	28.16179062908822	22.05982148426175
48-49	21.8816114684337	28.03193414573203	28.09669982636208	21.989754559472185
50-51	21.930790491743746	28.12578520644515	27.81945430891352	22.12396999289758
52-53	21.965438910220115	27.914324620171683	27.737895419394665	22.38234105021354
54-55	22.114459369785262	27.631773369121333	28.086302817467196	22.167464443626212
56-57	21.93841075608254	27.4933153207855	28.130125910914455	22.438148012217503
58-59	21.82999271509435	27.305646576869336	28.17742112693965	22.686939581096667
60-61	21.99991246536065	27.531128298029767	28.445630564886986	22.0233286717226
62-63	21.586527168551328	27.741414619462805	28.385991957920893	22.286066254064973
64-65	21.42838699646175	28.009034928113774	28.089773091170766	22.47280498425371
66-67	21.52656862861676	28.543219921984043	27.644108402428248	22.28610304697095
68-69	21.598057713592038	28.53996733570262	27.522745543837697	22.339229406867645
70-71	21.580291002057507	28.06625190541182	27.53394443520237	22.8195126573283
72-73	21.781377856073263	27.635650674070884	27.538611892695137	23.044359577160716
74-75	21.84273302169534	27.706657074188296	27.653639822421596	22.796970081694763
76-77	22.120718911552476	27.61512284524689	27.14004818763791	23.12411005556272
78-79	22.300666803492966	27.677258720054894	26.741209534495958	23.280864941956185
80-81	22.14629754404483	28.008038477991043	26.906441796391512	22.939222181572617
82-83	22.099045150460466	27.78665879797224	26.99463674807837	23.11965930348892
84-85	22.19670724816157	28.102945004384072	26.94128827068838	22.759059476765977
86-87	22.550758341427095	28.043634499570707	27.002875887438716	22.402731271563482
88-89	22.29145195176489	28.212082790449315	27.278693203890725	22.217772053895068
90-91	22.294148336428556	27.84032065379609	27.202356818891936	22.66317419088341
92-93	22.267172930990373	27.480025207193954	27.404553687579714	22.84824817423596
94-95	21.599299853837266	27.90970844931227	27.920960679724406	22.57003101712606
96-97	21.627487747092143	27.852655002585614	28.191442686720002	22.328414563602237
98-99	22.788712697104867	29.779665534244025	28.502383168585354	18.929238600065755
100	21.858012350508684	31.08835357751148	22.081434316621987	24.972199755357845
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1973.0
1	1682.5
2	2031.0
3	2805.0
4	3587.0
5	5528.0
6	7331.0
7	8763.0
8	9866.0
9	10117.0
10	9531.5
11	8698.0
12	9133.5
13	11448.5
14	17295.0
15	26265.0
16	34160.5
17	38862.0
18	40394.0
19	39144.5
20	36561.0
21	34078.5
22	32982.0
23	35021.0
24	40497.5
25	49645.5
26	62665.0
27	77380.0
28	93163.0
29	110501.5
30	127837.5
31	145292.0
32	164191.0
33	183319.0
34	202373.0
35	219552.0
36	238326.5
37	256799.5
38	270890.5
39	282678.0
40	294934.5
41	306095.5
42	313881.0
43	320439.0
44	324902.5
45	328406.0
46	328937.5
47	327725.5
48	325472.5
49	318826.5
50	310260.0
51	299127.5
52	284587.0
53	267625.5
54	249257.5
55	230352.5
56	210605.5
57	189757.5
58	167981.0
59	145383.0
60	120612.0
61	96975.0
62	76693.5
63	58993.5
64	44493.5
65	33513.5
66	25506.5
67	19848.0
68	15304.5
69	11141.0
70	7896.0
71	5858.0
72	4221.0
73	2878.5
74	1957.5
75	1279.5
76	817.5
77	528.0
78	359.0
79	253.0
80	167.0
81	111.0
82	83.5
83	57.0
84	37.5
85	27.0
86	18.0
87	13.0
88	10.5
89	7.5
90	6.0
91	5.0
92	3.5
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	6.38343697268821E-6
16-17	9.575155459032314E-4
18-19	0.001704377671707752
20-21	0.0
22-23	0.003351719483168686
24-25	2.937405548043886E-4
26-27	0.0014757035001195576
28-29	0.0051457822483936206
30-31	0.0014976829117259933
32-33	0.005568900028684968
34-35	0.01097356503581469
36-37	5.494414216906674E-4
38-39	6.494710123551468E-6
40-41	0.002513888581454463
42-43	0.0060559291689055855
44-45	0.0018236582185976002
46-47	0.0018269916534589
48-49	0.002977255791100071
50-51	0.01044564240802884
52-53	0.006416933276591259
54-55	0.009886191881959688
56-57	0.018706684729709974
58-59	0.012110323261969745
60-61	0.016413502086405976
62-63	0.014536963273604438
64-65	0.011091759580323176
66-67	0.013184475439402899
68-69	0.008663538174706067
70-71	0.009113850377543415
72-73	0.006317457325321031
74-75	0.010505363833073735
76-77	0.011480759085837882
78-79	0.006875797776046429
80-81	0.00394368866750405
82-83	0.0021502744966961146
84-85	6.18836150211027E-5
86-87	0.0037161863228539747
88-89	6.019175032461964E-4
90-91	4.7377700488777263E-4
92-93	0.0014108913636485226
94-95	0.001112754315223866
96-97	4.8559871808753844E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	155.0
20-21	562.0
22-23	1332.0
24-25	3015.0
26-27	4231.0
28-29	7257.0
30-31	17825.0
32-33	22814.0
34-35	30511.0
36-37	43531.0
38-39	31092.0
40-41	52455.0
42-43	61299.0
44-45	68431.0
46-47	66635.0
48-49	59971.0
50-51	34442.0
52-53	11117.0
54-55	11203.0
56-57	10827.0
58-59	34877.0
60-61	59655.0
62-63	57755.0
64-65	60829.0
66-67	64006.0
68-69	65904.0
70-71	65276.0
72-73	63976.0
74-75	64611.0
76-77	64396.0
78-79	69544.0
80-81	71582.0
82-83	70144.0
84-85	71937.0
86-87	76088.0
88-89	84427.0
90-91	92665.0
92-93	105182.0
94-95	130966.0
96-97	324396.0
98-99	595894.0
100-101	4999956.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.94245532597228
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.14290894728559	77.47102925155278
2	7.002701881919582	12.036587872959512
3	1.6373880634935918	4.221634514942347
4	0.5708260284055514	1.962327617805851
5	0.2615388456108906	1.1238645277460162
6	0.13034324884940376	0.6721211304769181
7	0.08027539109583356	0.48293449491200396
8	0.04789753448076616	0.329314537386997
9	0.02870310763320541	0.22201339909349968
>10	0.0951629628984121	1.231939483770373
>50	0.0016834540961339233	0.0926185700404728
>100	4.578591317808566E-4	0.06973590349937667
>500	9.014036505134184E-5	0.055196311161485066
>1k	2.2534734213357112E-5	0.028682384652412858
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.276687394537642E-5
2	0.0	5.106749578150568E-5	0.0	0.0	1.276687394537642E-5
3	0.0	5.106749578150568E-5	0.0	1.276687394537642E-5	1.276687394537642E-5
4	0.0	7.660124367225851E-5	0.0	1.276687394537642E-5	1.276687394537642E-5
5	0.0	7.660124367225851E-5	0.0	1.276687394537642E-5	1.276687394537642E-5
6	0.0	7.660124367225851E-5	0.0	1.276687394537642E-5	1.276687394537642E-5
7	0.0	7.660124367225851E-5	0.0	1.276687394537642E-5	1.276687394537642E-5
8	0.0	1.0213499156301135E-4	0.0	1.276687394537642E-5	1.276687394537642E-5
9	0.0	2.2980373101677554E-4	0.0	2.553374789075284E-5	1.276687394537642E-5
10-11	0.0	2.2980373101677554E-4	0.0	3.8300621836129256E-5	1.276687394537642E-5
12-13	0.0	2.2980373101677554E-4	0.0	6.38343697268821E-5	1.276687394537642E-5
14-15	0.0	2.2980373101677554E-4	0.0	7.021780669957031E-5	1.276687394537642E-5
16-17	0.0	2.3618716798946376E-4	0.0	1.1490186550838777E-4	1.276687394537642E-5
18-19	0.0	2.617209158802166E-4	0.0	1.5320248734451702E-4	1.9150310918064628E-5
20-21	6.38343697268821E-6	2.8087122679828125E-4	0.0	1.7873623523526988E-4	2.553374789075284E-5
22-23	1.276687394537642E-5	3.447055965251633E-4	0.0	1.9150310918064628E-4	3.8300621836129256E-5
24-25	1.276687394537642E-5	3.766227813886044E-4	0.0	2.042699831260227E-4	6.38343697268821E-5
26-27	1.276687394537642E-5	3.95773092306669E-4	0.0	3.5108903349785153E-4	6.38343697268821E-5
28-29	1.276687394537642E-5	3.95773092306669E-4	0.0	8.489971173675319E-4	6.38343697268821E-5
30-31	1.276687394537642E-5	4.3407371414279827E-4	0.0	0.002770411646146683	6.38343697268821E-5
32-33	1.276687394537642E-5	4.3407371414279827E-4	0.0	0.007806943417597681	6.38343697268821E-5
34-35	1.276687394537642E-5	4.915246468969922E-4	0.0	0.013686088869443521	7.660124367225851E-5
36-37	1.276687394537642E-5	5.48975579651186E-4	0.0	0.025342244781572192	7.660124367225851E-5
38-39	1.9150310918064628E-5	5.617424535965625E-4	0.0	0.17093567525464487	1.1490186550838777E-4
40-41	2.553374789075284E-5	5.745093275419388E-4	0.0	0.32203801183514746	1.1490186550838777E-4
42-43	2.553374789075284E-5	5.936596384600035E-4	0.0	0.3476228272216818	1.2128530248107598E-4
44-45	2.553374789075284E-5	6.511105712141974E-4	0.0	0.38297430117642917	1.276687394537642E-4
46-47	2.553374789075284E-5	7.021780669957031E-4	0.0	0.4209429842899786	1.276687394537642E-4
48-49	2.553374789075284E-5	7.085615039683912E-4	0.0	0.45648596135390657	1.276687394537642E-4
50-51	2.553374789075284E-5	8.298468064494673E-4	0.0	0.48933512801536005	1.276687394537642E-4
52-53	2.553374789075284E-5	8.298468064494673E-4	0.0	0.9142358432284055	1.4043561339914062E-4
54-55	2.553374789075284E-5	8.362302434221555E-4	0.0	1.4888856063837435	1.5320248734451702E-4
56-57	2.553374789075284E-5	8.426136803948436E-4	0.0	2.114130490984608	1.7235279826258165E-4
58-59	2.553374789075284E-5	8.489971173675319E-4	0.0	2.7558446429750085	1.7873623523526988E-4
60-61	2.553374789075284E-5	8.617639913129083E-4	0.0	2.9300805551445332	1.7873623523526988E-4
62-63	2.553374789075284E-5	8.681474282855965E-4	0.0	2.9379130323100213	1.7873623523526988E-4
64-65	2.553374789075284E-5	9.575155459032314E-4	0.0	2.9444688220809723	1.7873623523526988E-4
66-67	2.553374789075284E-5	9.702824198486079E-4	0.0	2.949926660692621	1.7873623523526988E-4
68-69	2.553374789075284E-5	0.001002199604712049	0.0	2.9549568090270992	2.2980373101677554E-4
70-71	2.553374789075284E-5	0.0010277333526028018	0.0	2.959872055496069	2.4257060496215196E-4
72-73	2.553374789075284E-5	0.0010405002265481782	0.0	2.963338261772239	2.681043528529048E-4
74-75	2.553374789075284E-5	0.0010788008483843075	0.0	2.966421461830047	2.681043528529048E-4
76-77	2.553374789075284E-5	0.0011617855290292542	0.0	2.968923769123341	2.681043528529048E-4
78-79	2.553374789075284E-5	0.0012128530248107598	0.0	2.9706345302320214	2.74487789825593E-4
80-81	2.553374789075284E-5	0.0012192364617834481	0.0	2.971228189870481	2.872546637709694E-4
82-83	2.553374789075284E-5	0.0012256198987561362	0.0	2.971687797332515	3.0640497468903405E-4
84-85	2.553374789075284E-5	0.0012256198987561362	0.0	2.9718601501307775	3.255552856070987E-4
86-87	2.553374789075284E-5	0.001251153646646889	0.0	2.9719431348114225	3.447055965251633E-4
88	2.553374789075284E-5	0.0013149880163737713	0.0	2.9719495182483953	3.447055965251633E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9440	0.0	49.410282	1
ATCTCGT	6450	0.0	42.33754	94
GTATCAA	16590	0.0	36.778152	1
TATGCCG	3470	0.0	34.714504	86-87
CCGTCTT	3635	0.0	34.615166	90-91
GCCGTCT	3695	0.0	34.364067	90-91
TATCTCG	7685	0.0	31.67332	94
ATGCCGT	3980	0.0	31.378178	88-89
TGCCGTC	3940	0.0	31.128185	88-89
TCAACGC	19605	0.0	30.310066	4
CTCGTAT	3870	0.0	30.153349	82-83
ATCAACG	19840	0.0	30.059492	3
CGTATGC	4010	0.0	29.846455	84-85
CAACGCA	20130	0.0	29.540941	5
TCTCGTA	4025	0.0	29.45657	82-83
TCGTATG	4165	0.0	29.255827	84-85
AACGCAG	20760	0.0	28.727373	6
TATCAAC	21240	0.0	28.280752	2
ACGCAGA	23720	0.0	25.088089	7
CGCAGAG	24220	0.0	24.587933	8
>>END_MODULE