FastQCFastQC Report
Fri 27 May 2016
SRR2139373_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139373_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8236858
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89550.048.623971
ATCTCGT70500.038.42615594
GTATCAA160700.035.7267531
TATCTCG85550.034.84076794
TATGCCG37950.034.4128686-87
GCCGTCT40850.034.26416490-91
CCGTCTT39950.033.66352590-91
ATGCCGT41350.033.24711288-89
TGCCGTC40500.032.90363788-89
CTCGTAT42000.030.32672982-83
TCTCGTA43100.030.29932482-83
TCGTATG44000.030.26740384-85
ATCAACG189200.029.482623
TCAACGC189000.029.4456564
CGTATGC44100.029.27431984-85
CAACGCA192600.028.9398615
AACGCAG198450.028.1083986
TATCAAC203800.027.6444342
CGCTATC132550.025.71385494
ACGCAGA228500.024.3366957
Produced by FastQC (version 0.11.5)