##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139373_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8236858
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09225884433118	33.0	31.0	34.0	30.0	34.0
2	32.27200213965082	33.0	31.0	34.0	30.0	34.0
3	32.291324070416174	34.0	31.0	34.0	30.0	34.0
4	36.020799071684856	37.0	35.0	37.0	35.0	37.0
5	35.89342343888895	37.0	35.0	37.0	35.0	37.0
6	35.91319979050264	37.0	35.0	37.0	35.0	37.0
7	35.85947408587109	37.0	35.0	37.0	35.0	37.0
8	35.84945837356914	37.0	35.0	37.0	35.0	37.0
9	37.631586947352986	39.0	37.0	39.0	35.0	39.0
10-11	37.51475605139727	39.0	37.0	39.0	35.0	39.0
12-13	37.49314004199174	39.0	37.0	39.0	35.0	39.0
14-15	38.75705238575195	40.0	38.0	41.0	34.5	41.0
16-17	38.7175392000202	40.0	38.0	41.0	34.0	41.0
18-19	38.73758586101643	40.0	38.0	41.0	34.0	41.0
20-21	38.72179506409658	40.0	38.0	41.0	34.0	41.0
22-23	38.644248203454254	40.0	38.0	41.0	34.0	41.0
24-25	38.58516491349148	40.0	38.0	41.0	34.0	41.0
26-27	38.417888881682565	40.0	38.0	41.0	34.0	41.0
28-29	38.317318790330816	40.0	38.0	41.0	34.0	41.0
30-31	38.17452572425472	40.0	38.0	41.0	33.5	41.0
32-33	38.00683487438444	40.0	37.0	41.0	33.0	41.0
34-35	38.094829056620306	40.0	37.5	41.0	33.0	41.0
36-37	38.07128898163204	40.0	37.5	41.0	33.0	41.0
38-39	38.04035641911806	40.0	37.0	41.0	33.0	41.0
40-41	37.97427869675357	40.0	37.0	41.0	33.0	41.0
42-43	37.8774469143022	40.0	37.0	41.0	33.0	41.0
44-45	37.93647004311232	40.0	37.0	41.0	33.0	41.0
46-47	37.93765443572934	40.0	37.0	41.0	33.0	41.0
48-49	37.89059078006618	40.0	37.0	41.0	33.0	41.0
50-51	37.8072647208695	40.0	37.0	41.0	33.0	41.0
52-53	37.63314571022383	40.0	37.0	41.0	32.0	41.0
54-55	37.52299320854095	39.5	36.0	41.0	32.0	41.0
56-57	37.3682858057325	39.0	36.0	41.0	31.5	41.0
58-59	37.246546490777504	39.0	36.0	41.0	31.0	41.0
60-61	37.040094905042615	39.0	35.0	41.0	31.0	41.0
62-63	36.84528684374131	39.0	35.0	40.0	31.0	41.0
64-65	36.56318579555935	38.0	35.0	40.0	31.0	41.0
66-67	36.291521600381685	38.0	35.0	40.0	30.5	41.0
68-69	35.992138048243746	37.0	34.0	40.0	30.0	41.0
70-71	35.70001914665571	37.0	34.0	39.0	30.0	41.0
72-73	35.40980743910647	36.0	34.0	39.0	30.0	41.0
74-75	35.02409517561819	36.0	34.0	39.0	29.0	40.0
76-77	33.7054347670462	34.5	32.0	36.5	28.0	39.0
78-79	34.34607494187611	35.0	33.5	37.0	29.0	39.0
80-81	34.233730184717444	35.0	34.0	37.0	29.0	39.0
82-83	33.937869596460274	35.0	33.0	36.0	29.0	38.5
84-85	33.7617249103651	35.0	33.0	36.0	29.0	37.0
86-87	33.52220311595167	35.0	33.0	35.5	29.0	37.0
88-89	33.417035090651936	35.0	33.0	35.0	29.0	36.5
90-91	33.290030644077	35.0	33.0	35.0	29.0	36.0
92-93	33.17217908201947	35.0	33.0	35.0	29.0	36.0
94-95	33.11690803100771	35.0	33.0	35.0	29.0	36.0
96-97	33.16287923480132	35.0	33.0	35.0	29.0	35.0
98-99	33.29012653102404	35.0	33.0	35.0	29.0	35.0
100	32.126923194778534	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	6.0
20	24.0
21	225.0
22	1231.0
23	4373.0
24	11525.0
25	25775.0
26	47015.0
27	74087.0
28	107858.0
29	143218.0
30	181655.0
31	224004.0
32	275364.0
33	345051.0
34	453402.0
35	620272.0
36	939480.0
37	1623667.0
38	2144965.0
39	976430.0
40	37231.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.86467194165542	20.601265676790835	14.694741611425133	23.839320770128612
2	14.376003568350942	21.57130789434515	41.585286525517375	22.467402011786533
3	17.679022753579098	26.693904398983204	31.00604623753378	24.62102660990392
4	12.388789511728865	18.70374601577446	39.55799650789172	29.34946796460495
5	12.896774473955967	38.198995296507476	36.03463845072963	12.86959177880692
6	29.197516820127284	38.67275604362732	19.226384623845647	12.903342512399751
7	25.93070075992569	32.99602833993253	23.73357656523883	17.339694334902944
8	24.077894750643996	36.82776854960957	21.61830396979042	17.47603272995601
9	25.498922040418808	17.023044466712914	21.454770739036658	36.02326275383162
10-11	23.728101176443737	26.957609321418435	29.518154859535034	19.796134642602798
12-13	24.708324746421802	24.57402595589398	29.365671313361926	21.3519779843223
14-15	21.812971645255992	26.005292309276186	27.03561236578317	25.146123679684656
16-17	21.4155648651587	29.083601950787656	27.66960808874784	21.831225095305804
18-19	21.44043640640711	28.387219630712497	29.216220134067832	20.956123828812565
20-21	22.285646018727338	27.599157343255264	29.058624353090735	21.056572284926666
22-23	21.960207089149677	27.524067489876437	28.865572855618616	21.650152565355274
24-25	21.778987038347903	27.624886512902115	28.944175733742554	21.65195071500743
26-27	21.741470898453898	27.82106762470386	28.79670817073063	21.64075330611162
28-29	21.647055118899075	27.96531574889778	28.811850600382904	21.575778531820237
30-31	22.00080576680203	27.719650125074025	28.747663212363012	21.531880895760935
32-33	21.78318652004295	27.8661177023657	28.68244906487228	21.668246712719068
34-35	21.87314481586135	27.923892644280023	28.70002653925376	21.502936000604862
36-37	21.86472062228558	27.865528338186024	28.64576492712602	21.623986112402374
38-39	21.6748631806643	27.95661499213179	28.66717589023707	21.70134593696684
40-41	22.050669175966515	27.76610699297506	28.630088174468828	21.5531356565896
42-43	21.81316344581431	27.85085671760672	28.456795655122196	21.879184181456772
44-45	21.898409880554357	27.946665973163316	28.274743977646132	21.880180168636198
46-47	21.895545953201616	27.887572818045374	28.248830218833387	21.968051009919623
48-49	21.832500727316997	28.12280607704045	28.152907937416416	21.891785258226136
50-51	21.849605159512574	28.230153558619175	27.89106154754919	22.029179734319058
52-53	21.893265556352056	28.028278951675745	27.812580793359672	22.26587469861253
54-55	22.020737398600335	27.772927777212043	28.16071223473702	22.045622589450605
56-57	21.873903365255533	27.529550159082756	28.242828624239856	22.35371785142185
58-59	21.721319665610036	27.40982310685701	28.269961785981906	22.598895441551047
60-61	21.89562160547269	27.591418834833597	28.536107303618408	21.9768522560753
62-63	21.476592698507055	27.788955741562	28.537035451954356	22.19741610797659
64-65	21.32871478966118	28.055753897397693	28.22035738172543	22.395173931215695
66-67	21.37609119210473	28.63729464646071	27.74654265514855	22.240071506286014
68-69	21.500265796864447	28.645110274198153	27.56546758963961	22.289156339297787
70-71	21.497999733417004	28.16879903259809	27.58032188314889	22.752879350836018
72-73	21.67449316961321	27.661196760179852	27.609253863200884	23.055056207006057
74-75	21.76490798991409	27.72348311675927	27.749693580189433	22.76191531313721
76-77	22.02476642281233	27.692441152924324	27.177964094245965	23.104828330017384
78-79	22.260543669699295	27.74189205593628	26.742686581769888	23.25487769259454
80-81	22.055757947394834	28.076767329088163	26.912805353364583	22.954669370152416
82-83	22.03221059904549	27.867626172802495	27.015739726953846	23.084423501198163
84-85	22.137325630196035	28.210343878511246	26.970433398687625	22.681897092605094
86-87	22.514995536045536	28.11098282394786	27.020687377181414	22.353334262825193
88-89	22.24949161200522	28.288922084138168	27.322600483274417	22.138985820582192
90-91	22.239975639580088	27.910617723158023	27.219212591215225	22.630194046046665
92-93	22.21798600873884	27.536434990700272	27.422033817364504	22.823545183196376
94-95	21.522585364330098	27.995914442669278	27.979240969124593	22.502259223876035
96-97	21.571889637722013	27.8835298225278	28.258746368878708	22.285834170871478
98-99	22.692828427928298	29.838496284549276	28.590094429211483	18.878580858310944
100	21.81313104230186	31.09776463558504	22.112028681303528	24.977075640809574
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1912.0
1	1697.5
2	2131.0
3	2977.0
4	3847.5
5	5817.0
6	7616.5
7	9006.0
8	9977.5
9	9984.5
10	9409.5
11	8828.0
12	9480.0
13	11952.5
14	17657.0
15	26378.5
16	34200.0
17	39081.5
18	40922.5
19	39900.0
20	37290.5
21	34780.5
22	33662.0
23	35434.5
24	41277.5
25	50727.0
26	64115.0
27	80065.5
28	97056.5
29	116033.0
30	134676.5
31	153757.0
32	175235.0
33	196314.0
34	216809.0
35	235768.0
36	256257.0
37	276725.5
38	291197.5
39	303952.0
40	319426.5
41	332124.0
42	339588.5
43	346591.0
44	352434.0
45	356384.5
46	357046.0
47	356054.5
48	353357.0
49	345006.0
50	333458.0
51	318399.0
52	299733.0
53	279160.0
54	258051.5
55	236293.5
56	212984.5
57	188915.0
58	165024.5
59	141360.5
60	116908.0
61	93503.5
62	73387.5
63	56170.5
64	41806.5
65	31483.0
66	23964.5
67	18453.5
68	14115.5
69	10184.5
70	7309.5
71	5326.0
72	3740.5
73	2544.0
74	1721.5
75	1189.5
76	795.0
77	499.0
78	311.5
79	204.0
80	142.0
81	100.5
82	77.5
83	52.0
84	33.5
85	22.0
86	16.5
87	15.0
88	13.5
89	9.5
90	7.0
91	7.5
92	6.5
93	3.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	6.0702758260492045E-6
14-15	0.0
16-17	0.0010015955112981187
18-19	0.00160255281807699
20-21	0.0
22-23	0.0033815921543662217
24-25	2.1253641811524405E-4
26-27	0.0016220013856873261
28-29	0.005069651171527088
30-31	0.0015947086835478958
32-33	0.005455010744662663
34-35	0.010957337697496065
36-37	5.472466636861194E-4
38-39	4.9431158584795816E-5
40-41	0.0026405355130280916
42-43	0.005744412682611938
44-45	0.0016222160531723288
46-47	0.0020005388712666617
48-49	0.0028281964501249484
50-51	0.010244616295380864
52-53	0.006383425870256935
54-55	0.009225291047515847
56-57	0.017820144470317233
58-59	0.011192449001873854
60-61	0.01550078378220965
62-63	0.013861596959384792
64-65	0.010924230699160792
66-67	0.012405999592673052
68-69	0.008301844439643888
70-71	0.008307675590769953
72-73	0.005791158733786057
74-75	0.009892805042119441
76-77	0.011378810148907344
78-79	0.006294577500447595
80-81	0.003817901155117564
82-83	0.002091031369053072
84-85	5.174915852171875E-5
86-87	0.003754726879521286
88-89	4.3175014846145456E-4
90-91	5.377208073678198E-4
92-93	0.0015059746226889824
94-95	0.0012874489699335368
96-97	3.343074726933987E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	170.0
20-21	632.0
22-23	1433.0
24-25	3107.0
26-27	4471.0
28-29	7873.0
30-31	19309.0
32-33	24573.0
34-35	32898.0
36-37	47253.0
38-39	33372.0
40-41	56590.0
42-43	65860.0
44-45	73132.0
46-47	70796.0
48-49	64192.0
50-51	36433.0
52-53	11788.0
54-55	11822.0
56-57	11421.0
58-59	37349.0
60-61	64159.0
62-63	63357.0
64-65	65883.0
66-67	69543.0
68-69	71095.0
70-71	70554.0
72-73	68915.0
74-75	70655.0
76-77	69461.0
78-79	74831.0
80-81	76592.0
82-83	74717.0
84-85	77523.0
86-87	81999.0
88-89	90325.0
90-91	98051.0
92-93	111206.0
94-95	137713.0
96-97	339902.0
98-99	624383.0
100-101	5221520.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.07078733782407
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.0780771406666	77.53091021374425
2	7.089529777956249	12.204028196872867
3	1.6497873514602117	4.259954888404917
4	0.5649837388582671	1.9451438094639457
5	0.24372519840866771	1.048880986055071
6	0.13629177468677908	0.7038444212976238
7	0.07643568292774683	0.46052155872068135
8	0.03950555095004998	0.27202190995882597
9	0.02812391904271682	0.21785830695286737
>10	0.09182672421436737	1.1836189231513967
>50	0.0010804120051931562	0.058720148290035454
>100	5.698857965681821E-4	0.07822087136914817
>500	6.284302656541279E-5	0.03627576571840837
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.2140551652098409E-5	0.0	0.0	0.0
3	0.0	1.2140551652098409E-5	0.0	0.0	0.0
4	0.0	4.8562206608393636E-5	0.0	0.0	0.0
5	0.0	4.8562206608393636E-5	0.0	2.4281103304196818E-5	0.0
6	0.0	4.8562206608393636E-5	0.0	2.4281103304196818E-5	0.0
7	0.0	4.8562206608393636E-5	0.0	3.642165495629523E-5	1.2140551652098409E-5
8	0.0	6.070275826049205E-5	0.0	3.642165495629523E-5	1.2140551652098409E-5
9	0.0	8.498386156468886E-5	0.0	3.642165495629523E-5	1.2140551652098409E-5
10-11	0.0	8.498386156468886E-5	0.0	4.856220660839364E-5	1.2140551652098409E-5
12-13	0.0	8.498386156468886E-5	0.0	7.284330991259046E-5	1.2140551652098409E-5
14-15	0.0	1.4568661982518091E-4	0.0	7.891358573863966E-5	1.2140551652098409E-5
16-17	0.0	1.5782717147727932E-4	0.0	9.105413739073807E-5	1.2140551652098409E-5
18-19	6.0702758260492045E-6	1.6996772312937773E-4	0.0	1.0319468904283648E-4	1.2140551652098409E-5
20-21	1.2140551652098409E-5	1.9424882643357454E-4	0.0	1.214055165209841E-4	2.4281103304196818E-5
22-23	1.2140551652098409E-5	2.0638937808567295E-4	0.0	1.335460681730825E-4	2.4281103304196818E-5
24-25	1.2140551652098409E-5	2.306704813898698E-4	0.0	2.0638937808567295E-4	2.4281103304196818E-5
26-27	1.2140551652098409E-5	2.549515846940666E-4	0.0	3.2779489460665705E-4	2.4281103304196818E-5
28-29	1.2140551652098409E-5	2.549515846940666E-4	0.0	8.376980639947903E-4	2.4281103304196818E-5
30-31	1.2140551652098409E-5	3.1565434295455864E-4	0.0	0.0029015918448515197	2.4281103304196818E-5
32-33	1.2140551652098409E-5	3.1565434295455864E-4	0.0	0.0077335314023866866	2.4281103304196818E-5
34-35	1.2140551652098409E-5	3.763571012150507E-4	0.0	0.014058758813129957	2.4281103304196818E-5
36-37	1.2140551652098409E-5	4.492004111276411E-4	0.0	0.026618159497225763	2.4281103304196818E-5
38-39	1.2140551652098409E-5	4.6134096277973953E-4	0.0	0.1785510931474113	2.4281103304196818E-5
40-41	1.2140551652098409E-5	4.73481514431838E-4	0.0	0.3366453567610368	2.4281103304196818E-5
42-43	1.2140551652098409E-5	4.856220660839364E-4	0.0	0.36343955425721797	2.4281103304196818E-5
44-45	1.2140551652098409E-5	5.584653759965268E-4	0.0	0.39911456528690914	2.4281103304196818E-5
46-47	1.2140551652098409E-5	6.191681342570188E-4	0.0	0.43590043679276735	2.4281103304196818E-5
48-49	1.2140551652098409E-5	6.191681342570188E-4	0.0	0.4708227336200284	2.4281103304196818E-5
50-51	1.2140551652098409E-5	6.555897892133141E-4	0.0	0.5046402402469485	3.642165495629523E-5
52-53	2.4281103304196818E-5	6.555897892133141E-4	0.0	0.9510968867012155	3.642165495629523E-5
54-55	2.4281103304196818E-5	6.555897892133141E-4	0.0	1.5538509951245971	3.642165495629523E-5
56-57	2.4281103304196818E-5	6.677303408654126E-4	0.0	2.2069519712492314	3.642165495629523E-5
58-59	2.4281103304196818E-5	6.677303408654126E-4	0.0	2.8736442949483894	3.642165495629523E-5
60-61	2.4281103304196818E-5	6.738006166914618E-4	0.0	3.0526870804377104	3.642165495629523E-5
62-63	2.4281103304196818E-5	6.798708925175109E-4	0.0	3.0605602281840962	4.856220660839364E-5
64-65	2.4281103304196818E-5	7.527142024301014E-4	0.0	3.0671889693861423	6.070275826049205E-5
66-67	2.4281103304196818E-5	7.587844782561506E-4	0.0	3.0729010989384546	6.070275826049205E-5
68-69	2.4281103304196818E-5	7.830655815603474E-4	0.0	3.078291503871986	6.677303408654125E-5
70-71	2.4281103304196818E-5	7.952061332124458E-4	0.0	3.0827835079832626	7.284330991259046E-5
72-73	2.4281103304196818E-5	8.073466848645442E-4	0.0	3.086031105550199	7.891358573863966E-5
74-75	2.4281103304196818E-5	8.194872365166427E-4	0.0	3.0892787031171354	8.498386156468886E-5
76-77	2.4281103304196818E-5	8.31627788168741E-4	0.0	3.0921681544103343	8.498386156468886E-5
78-79	2.4281103304196818E-5	8.376980639947903E-4	0.0	3.0939892371581497	9.105413739073807E-5
80-81	2.4281103304196818E-5	8.376980639947903E-4	0.0	3.0946084052924063	9.712441321678727E-5
82-83	2.4281103304196818E-5	8.376980639947903E-4	0.0	3.095179011220055	9.712441321678727E-5
84-85	2.4281103304196818E-5	8.559088914729378E-4	0.0	3.0954157519772707	9.712441321678727E-5
86-87	2.4281103304196818E-5	8.741197189510854E-4	0.0	3.0955128763904876	1.0319468904283648E-4
88	2.4281103304196818E-5	8.862602706031839E-4	0.0	3.0955128763904876	1.0926496486888568E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8955	0.0	48.62397	1
ATCTCGT	7050	0.0	38.426155	94
GTATCAA	16070	0.0	35.726753	1
TATCTCG	8555	0.0	34.840767	94
TATGCCG	3795	0.0	34.41286	86-87
GCCGTCT	4085	0.0	34.264164	90-91
CCGTCTT	3995	0.0	33.663525	90-91
ATGCCGT	4135	0.0	33.247112	88-89
TGCCGTC	4050	0.0	32.903637	88-89
CTCGTAT	4200	0.0	30.326729	82-83
TCTCGTA	4310	0.0	30.299324	82-83
TCGTATG	4400	0.0	30.267403	84-85
ATCAACG	18920	0.0	29.48262	3
TCAACGC	18900	0.0	29.445656	4
CGTATGC	4410	0.0	29.274319	84-85
CAACGCA	19260	0.0	28.939861	5
AACGCAG	19845	0.0	28.108398	6
TATCAAC	20380	0.0	27.644434	2
CGCTATC	13255	0.0	25.713854	94
ACGCAGA	22850	0.0	24.336695	7
>>END_MODULE