FastQCFastQC Report
Fri 27 May 2016
SRR2139372_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139372_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6234376
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71150.051.0510181
ATCTCGT51650.041.2057494
GTATCAA134750.033.947841
TATGCCG29650.031.6808986-87
TCGTATG31650.031.1621984-85
TCTCGTA32100.029.91266382-83
TCAACGC179950.029.7995974
GCCGTCT34700.029.50123690-91
ATCAACG182150.029.488023
CTCGTAT31950.029.24521882-83
TATCTCG60750.028.90507594
CCGTCTT33950.028.88669290-91
CAACGCA186050.028.822565
ATGCCGT34600.028.78789988-89
TATCAAC189000.028.4898432
CGTATGC33400.028.0490784-85
AACGCAG192500.027.9012186
TGCCGTC34700.027.48344888-89
ACGCAGA219750.024.441347
CGCAGAG225750.023.7917358
Produced by FastQC (version 0.11.5)