##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139372_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6234376
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.594012295697276	18.0	18.0	30.0	18.0	33.0
2	30.21699252659769	31.0	27.0	33.0	27.0	33.0
3	31.921976313266956	33.0	31.0	33.0	29.0	33.0
4	32.3012750594446	33.0	33.0	33.0	31.0	34.0
5	32.825676058036926	33.0	33.0	34.0	32.0	34.0
6	36.852491412131705	38.0	37.0	38.0	35.0	38.0
7	37.093221839683714	38.0	38.0	38.0	36.0	38.0
8	37.128319658615396	38.0	38.0	38.0	36.0	38.0
9	37.25589698151026	38.0	38.0	38.0	36.0	38.0
10-11	37.287104916354096	38.0	38.0	38.0	37.0	38.0
12-13	37.306973304144634	38.0	38.0	38.0	37.0	38.0
14-15	37.31109320323317	38.0	38.0	38.0	37.0	38.0
16-17	37.30763223135723	38.0	38.0	38.0	37.0	38.0
18-19	37.30783714360507	38.0	38.0	38.0	37.0	38.0
20-21	37.27675115096504	38.0	38.0	38.0	37.0	38.0
22-23	37.28998481567663	38.0	38.0	38.0	37.0	38.0
24-25	37.31246669313629	38.0	38.0	38.0	37.0	38.0
26-27	37.288075600961406	38.0	38.0	38.0	37.0	38.0
28-29	37.26743509480431	38.0	38.0	38.0	37.0	38.0
30-31	37.26826232925073	38.0	38.0	38.0	37.0	38.0
32-33	37.21086083065276	38.0	38.0	38.0	36.5	38.0
34-35	37.22470204665247	38.0	38.0	38.0	36.5	38.0
36-37	37.221221786137704	38.0	38.0	38.0	36.5	38.0
38-39	37.21388258436655	38.0	38.0	38.0	36.5	38.0
40-41	37.20527297014335	38.0	38.0	38.0	36.5	38.0
42-43	37.179882275659295	38.0	38.0	38.0	36.0	38.0
44-45	37.15444873355811	38.0	38.0	38.0	36.0	38.0
46-47	37.121563803686854	38.0	38.0	38.0	36.0	38.0
48-49	37.074898813638356	38.0	38.0	38.0	36.0	38.0
50-51	37.04275180065296	38.0	38.0	38.0	36.0	38.0
52-53	37.008516733382116	38.0	38.0	38.0	36.0	38.0
54-55	36.986227373842105	38.0	38.0	38.0	36.0	38.0
56-57	36.95715152167232	38.0	38.0	38.0	36.0	38.0
58-59	36.966818045402995	38.0	38.0	38.0	36.0	38.0
60-61	36.99168278153691	38.0	38.0	38.0	36.0	38.0
62-63	36.99980573614587	38.0	38.0	38.0	36.0	38.0
64-65	36.99863281626379	38.0	38.0	38.0	36.0	38.0
66-67	37.00490751094526	38.0	38.0	38.0	36.0	38.0
68-69	37.00343169008866	38.0	38.0	38.0	36.0	38.0
70-71	36.99398779459483	38.0	38.0	38.0	36.0	38.0
72-73	36.96956265772636	38.0	38.0	38.0	36.0	38.0
74-75	36.95994149451888	38.0	38.0	38.0	36.0	38.0
76-77	36.95578497883469	38.0	38.0	38.0	36.0	38.0
78-79	36.92433905696427	38.0	38.0	38.0	35.0	38.0
80-81	36.897652900288534	38.0	38.0	38.0	35.0	38.0
82-83	36.88229628520685	38.0	38.0	38.0	35.0	38.0
84-85	36.849487433332754	38.0	38.0	38.0	35.0	38.0
86-87	36.83482876639403	38.0	38.0	38.0	35.0	38.0
88-89	36.83797166749943	38.0	38.0	38.0	35.0	38.0
90-91	36.815459313798726	38.0	38.0	38.0	35.0	38.0
92-93	36.820036316391004	38.0	38.0	38.0	35.0	38.0
94-95	36.74867925681558	38.0	38.0	38.0	34.5	38.0
96-97	36.68759332386682	38.0	38.0	38.0	34.0	38.0
98-99	36.783446450586084	38.0	38.0	38.0	34.5	38.0
100	35.19241065625853	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	7.0
19	31.0
20	91.0
21	219.0
22	626.0
23	1567.0
24	3563.0
25	6714.0
26	11548.0
27	18522.0
28	28231.0
29	40968.0
30	56379.0
31	75157.0
32	98986.0
33	133449.0
34	195834.0
35	342990.0
36	1005780.0
37	4213714.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	36.40678714277099	18.391335395876027	21.82393233901837	23.377945122334616
2	14.597604298448779	21.112294359753676	40.840107605583285	23.44999373621426
3	18.32059856511702	25.891075546293646	30.471582079746234	25.3167438088431
4	12.827089671845265	18.027385579567227	37.94678088071685	31.19874386787066
5	13.623372152821378	37.372833587364056	35.397168565293704	13.60662569452086
6	30.414994685107665	37.34809408939381	18.50389349748146	13.73301772801706
7	26.85789674185464	32.59345162907268	22.794041102756893	17.75461052631579
8	25.197453602413454	35.89125840340717	20.745684892922725	18.165603101256643
9	26.17211409770601	16.535528174752372	20.654176135670994	36.63818159187062
10-11	24.77483712884818	26.26466546130679	28.373609483932317	20.586887925912713
12-13	25.65231869235991	23.920212704527284	28.51401647895475	21.913452124158056
14-15	22.77790110830659	25.066462144727875	26.108106087922835	26.0475306590427
16-17	22.307380883026624	28.237044092303705	26.75197966885539	22.70359535581428
18-19	22.435942265914022	27.402461770031195	28.47066811498055	21.690927849074228
20-21	23.291401814351975	26.465443068755846	28.292354608991687	21.950800507900496
22-23	23.143756211834713	26.352230999084387	28.15484207102008	22.349170718060822
24-25	22.79669029007325	26.55265873811915	28.171299361689172	22.47935161011842
26-27	22.657909042844746	26.67581564068661	28.18529207235893	22.480983244109712
28-29	22.629139180187043	26.68160125249603	28.227498584988247	22.461760982328684
30-31	22.803404669760653	26.58338729820991	28.100731866516593	22.512476165512847
32-33	22.698621566637257	26.689366634377965	28.084187029048806	22.527824769935968
34-35	22.815439222957668	26.66902047059577	28.064935317290647	22.45060498915592
36-37	22.809929548891596	26.621816935833	28.04363574829146	22.524617766983944
38-39	22.638957327888882	26.693924105095245	28.10823689861061	22.558881668405263
40-41	22.842203118842125	26.490326519450274	28.18082486335758	22.486645498350022
42-43	22.64218462796377	26.633099902723362	27.933202882718756	22.791512586594116
44-45	22.67337013717506	26.66097151456526	27.91641697813833	22.749241370121347
46-47	22.804814046532794	26.69568669003991	27.805112993740376	22.69438626968692
48-49	22.723806882876566	26.905060403456826	27.603816141751775	22.767316571914833
50-51	22.714183913738744	27.00978100521078	27.39968455538521	22.87635052566527
52-53	22.76849568730439	26.779983275874024	27.34544733157073	23.10607370525086
54-55	22.877416234870246	26.613251772021616	27.571512787630432	22.937819205477705
56-57	22.73810215437661	26.389922846146934	27.72721238893216	23.14476261054429
58-59	22.609812724842556	26.244873716422525	27.728762780857817	23.4165507778771
60-61	22.764612232370297	26.403251769590508	28.063379306818874	22.76875669122032
62-63	22.446497777983698	26.601963608119878	27.98615577032249	22.965382843573934
64-65	22.27305517906471	26.81863722626783	27.695444066844583	23.212863527822883
66-67	22.328563376278687	27.264141011304837	27.35007215807449	23.057223454341987
68-69	22.414130103335363	27.28545609602347	27.173505305484497	23.12690849515667
70-71	22.430700222675462	26.888941624830725	27.18719973204356	23.493158420450257
72-73	22.632273299067332	26.5162280271127	27.075153628111686	23.776345045708283
74-75	22.626197849842743	26.60984923697519	27.294783616654495	23.469169296527568
76-77	23.057738983477464	26.442719476456734	26.73022779166142	23.76931374840438
78-79	23.10911676516661	26.54136793825696	26.33027916022121	24.019236136355214
80-81	22.97817738459856	26.89657571429902	26.471988902164075	23.65325799893835
82-83	22.986583850931677	26.68115942028986	26.502213020473892	23.830043708304576
84-85	22.998680345680082	27.052913558284608	26.443725374688558	23.504680721346748
86-87	23.428681610530873	26.878571379859682	26.540027433653034	23.1527195759564
88-89	23.13814953825982	27.050645511551767	26.74176722411718	23.069437726071232
90-91	23.2146039894237	26.694536513059656	26.644256841287632	23.446602656229008
92-93	23.199262522746864	26.389857293362706	26.79435877789484	23.616521405995595
94-95	22.63289948297009	26.688192419698236	27.279749424998663	23.399158672333005
96-97	22.79160947987819	26.619923024082475	27.4153120775948	23.17315541844453
98-99	23.98201689434968	28.541046770831347	27.60678853081932	19.87014780399965
100	22.938251834111785	29.443251569168172	21.4358177495916	26.182678847128443
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1161.0
1	1381.5
2	1937.5
3	2352.0
4	2763.0
5	3382.5
6	4003.0
7	6074.0
8	7898.5
9	8017.5
10	8069.5
11	8286.0
12	9084.5
13	10821.5
14	14550.5
15	18800.5
16	20982.5
17	20951.5
18	19163.0
19	16595.0
20	14271.5
21	12822.5
22	12939.0
23	14961.5
24	18697.0
25	24375.0
26	32298.0
27	41675.5
28	52456.5
29	65364.5
30	78083.5
31	90901.5
32	104836.5
33	118313.0
34	131807.5
35	143973.5
36	156396.0
37	170335.5
38	182529.0
39	192577.0
40	202872.0
41	212925.5
42	221618.0
43	231169.0
44	240493.5
45	250061.5
46	257539.5
47	262411.0
48	265821.5
49	265074.0
50	263190.5
51	260847.0
52	255132.5
53	246059.5
54	235737.0
55	222971.5
56	206135.5
57	186485.0
58	165490.0
59	142887.5
60	119032.5
61	96011.5
62	75313.0
63	57560.5
64	43005.0
65	31784.5
66	23582.0
67	17873.0
68	13487.0
69	9710.0
70	6840.0
71	4873.0
72	3315.0
73	2143.5
74	1339.0
75	795.0
76	496.5
77	288.5
78	179.0
79	117.5
80	71.5
81	42.0
82	23.0
83	18.5
84	16.5
85	12.5
86	8.0
87	5.0
88	3.5
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.00203709240507791
3	0.0
4	0.0
5	0.0035609016844668972
6	1.1228068374445174E-4
7	1.6040097677778818E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	7.375024071259122E-5
44-45	2.4735236094118234E-5
46-47	0.0
48-49	0.0
50-51	2.5251723914148515E-5
52-53	0.0010796172149688252
54-55	0.0021436391853529692
56-57	0.002727433548361492
58-59	0.001816449854367189
60-61	0.004339603621794118
62-63	0.0017267376173465668
64-65	0.0017724775536368058
66-67	1.992953954618532E-4
68-69	1.571359219614125E-4
70-71	2.2869129296603636E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	7.246745056519629E-5
80-81	2.6477259914021946E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	3.798274045282453E-5
92-93	2.490176731673688E-4
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	51.0
20-21	190.0
22-23	344.0
24-25	479.0
26-27	1269.0
28-29	3266.0
30-31	10571.0
32-33	12951.0
34-35	17706.0
36-37	27672.0
38-39	17660.0
40-41	32175.0
42-43	35603.0
44-45	42336.0
46-47	42774.0
48-49	39949.0
50-51	20456.0
52-53	3600.0
54-55	3226.0
56-57	3141.0
58-59	21396.0
60-61	38943.0
62-63	37752.0
64-65	39993.0
66-67	42539.0
68-69	43095.0
70-71	42707.0
72-73	39779.0
74-75	40178.0
76-77	42049.0
78-79	43301.0
80-81	43046.0
82-83	41026.0
84-85	40655.0
86-87	41807.0
88-89	43818.0
90-91	45190.0
92-93	45826.0
94-95	58947.0
96-97	216719.0
98-99	494157.0
100-101	4416034.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.477624218949
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.33869992042825	73.74304795948011
2	7.998840247195112	13.354483606855174
3	2.062607464341541	5.165447124585073
4	0.7319228354351679	2.443967176548984
5	0.3601749599116887	1.5033274978291487
6	0.18161537511533982	0.909649202175708
7	0.10086219397325781	0.5893815428478851
8	0.06453519267247158	0.4309795650248044
9	0.041423457086422054	0.31121386060590905
>10	0.11777864001381627	1.4465179662872323
>50	0.0012482609068011489	0.06502689740206276
>100	2.914529201754196E-4	0.03695760035786004
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.6040097677778818E-5	0.0	0.0	0.0
3	0.0	1.6040097677778818E-5	0.0	0.0	0.0
4	0.0	1.6040097677778818E-5	0.0	1.6040097677778818E-5	1.6040097677778818E-5
5	0.0	1.6040097677778818E-5	0.0	1.6040097677778818E-5	1.6040097677778818E-5
6	0.0	1.6040097677778818E-5	0.0	1.6040097677778818E-5	1.6040097677778818E-5
7	0.0	1.6040097677778818E-5	0.0	1.6040097677778818E-5	1.6040097677778818E-5
8	0.0	1.6040097677778818E-5	0.0	3.2080195355557636E-5	3.2080195355557636E-5
9	0.0	4.8120293033336454E-5	0.0	3.2080195355557636E-5	3.2080195355557636E-5
10-11	0.0	4.8120293033336454E-5	0.0	3.2080195355557636E-5	3.2080195355557636E-5
12-13	0.0	4.8120293033336454E-5	0.0	1.1228068374445173E-4	3.2080195355557636E-5
14-15	0.0	1.0426063490556232E-4	0.0	1.1228068374445173E-4	4.8120293033336454E-5
16-17	0.0	1.2030073258334113E-4	0.0	1.2030073258334113E-4	4.8120293033336454E-5
18-19	0.0	1.3634083026111996E-4	0.0	1.2832078142223054E-4	6.416039071111527E-5
20-21	0.0	1.684210256166776E-4	0.0	1.2832078142223054E-4	6.416039071111527E-5
22-23	0.0	1.9248117213334581E-4	0.0	1.4436087910000937E-4	7.218043955000469E-5
24-25	0.0	2.566415628444611E-4	0.0	1.4436087910000937E-4	8.020048838889409E-5
26-27	0.0	2.8872175820001875E-4	0.0	2.0852126981112465E-4	8.020048838889409E-5
28-29	0.0	2.8872175820001875E-4	0.0	3.92982393105581E-4	8.020048838889409E-5
30-31	0.0	3.2080195355557636E-4	0.0	0.0012511276188667479	8.020048838889409E-5
32-33	0.0	3.2080195355557636E-4	0.0	0.0031839593890390953	8.020048838889409E-5
34-35	0.0	4.090224907833599E-4	0.0	0.00557393394302814	9.624058606667291E-5
36-37	0.0	4.8120293033336456E-4	0.0	0.011693231207100759	1.0426063490556232E-4
38-39	0.0	5.052630768500328E-4	0.0	0.141922784252987	1.1228068374445173E-4
40-41	0.0	5.934836140778163E-4	0.0	0.27674782528355685	1.1228068374445173E-4
42-43	0.0	6.015036629167057E-4	0.0	0.29181749705183	1.1228068374445173E-4
44-45	0.0	6.416039071111527E-4	0.0	0.3097102260113923	1.1228068374445173E-4
46-47	0.0	6.736841024667104E-4	0.0	0.3303859119180492	1.2832078142223054E-4
48-49	0.0	6.736841024667104E-4	0.0	0.34989067069422825	1.4436087910000937E-4
50-51	0.0	8.020048838889409E-4	0.0	0.36978841186351286	1.5238092793889876E-4
52-53	0.0	8.180449815667197E-4	0.0	0.7661472455302665	1.684210256166776E-4
54-55	0.0	8.180449815667197E-4	0.0	1.2960479124133675	1.76441074455567E-4
56-57	0.0	8.340850792444986E-4	0.0	1.8705560909383714	1.76441074455567E-4
58-59	0.0	8.340850792444986E-4	0.0	2.465507373953704	1.76441074455567E-4
60-61	0.0	8.501251769222774E-4	0.0	2.6217700055306254	1.76441074455567E-4
62-63	0.0	8.661652746000562E-4	0.0	2.626325393271115	1.76441074455567E-4
64-65	0.0	0.0010426063490556231	0.0	2.6309930616953485	1.76441074455567E-4
66-67	0.0	0.001074686544411181	0.0	2.6356206298753877	1.76441074455567E-4
68-69	0.0	0.0010827065932500703	0.0	2.6397669951250933	1.76441074455567E-4
70-71	0.0	0.0010907266420889597	0.0	2.6431594757839436	1.76441074455567E-4
72-73	0.0	0.0010907266420889597	0.0	2.645838172096133	1.9248117213334581E-4
74-75	0.0	0.001098746690927849	0.0	2.647771003866305	1.9248117213334581E-4
76-77	0.0	0.0011388469351222962	0.0	2.6498321564179	1.9248117213334581E-4
78-79	0.0	0.0011869672281556326	0.0	2.651147444427478	1.9248117213334581E-4
80-81	0.0	0.0012270674723500797	0.0	2.651628647357811	2.0050122097223523E-4
82-83	0.0	0.0012671677165445267	0.0	2.6518612287741385	2.2456136748890345E-4
84-85	0.0	0.0013152880095778632	0.0	2.6520938101904665	2.2456136748890345E-4
86-87	0.0	0.0013233080584167526	0.0	2.652133910434661	2.2456136748890345E-4
88	0.0	0.0014275686933223149	0.0	2.652133910434661	2.2456136748890345E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7115	0.0	51.051018	1
ATCTCGT	5165	0.0	41.20574	94
GTATCAA	13475	0.0	33.94784	1
TATGCCG	2965	0.0	31.68089	86-87
TCGTATG	3165	0.0	31.16219	84-85
TCTCGTA	3210	0.0	29.912663	82-83
TCAACGC	17995	0.0	29.799597	4
GCCGTCT	3470	0.0	29.501236	90-91
ATCAACG	18215	0.0	29.48802	3
CTCGTAT	3195	0.0	29.245218	82-83
TATCTCG	6075	0.0	28.905075	94
CCGTCTT	3395	0.0	28.886692	90-91
CAACGCA	18605	0.0	28.82256	5
ATGCCGT	3460	0.0	28.787899	88-89
TATCAAC	18900	0.0	28.489843	2
CGTATGC	3340	0.0	28.04907	84-85
AACGCAG	19250	0.0	27.901218	6
TGCCGTC	3470	0.0	27.483448	88-89
ACGCAGA	21975	0.0	24.44134	7
CGCAGAG	22575	0.0	23.791735	8
>>END_MODULE