FastQCFastQC Report
Fri 27 May 2016
SRR2139371_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139371_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7093792
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88250.046.8587651
GTATCAA159900.036.9414521
TCAACGC188850.030.597024
ATCAACG190200.030.4249533
CAACGCA194350.029.7532125
TATCAAC200600.029.146972
AACGCAG200850.028.7903256
ACGCAGA229550.025.190757
CGCAGAG234200.024.6722768
GCAGAGT273150.021.1855269
GTACATG207200.020.827481
TACATGG206500.020.711122
ACATGGG208400.020.0282783
GAGTACT152000.019.47408112-13
AGAGTAC242250.019.0184510-11
CAGAGTA265950.018.39627610-11
GTGGTAT45250.017.9172651
CATGGGG153500.017.5221614
GTACTTT169700.017.49444614-15
AGTACTT168500.017.0833912-13
Produced by FastQC (version 0.11.5)