FastQCFastQC Report
Fri 27 May 2016
SRR2139370_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139370_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7740669
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97200.051.5019231
GTATCAA174900.037.941921
TCAACGC215450.030.2632754
ATCAACG217000.030.0075723
TATCAAC222300.029.407922
CAACGCA221850.029.3322245
AACGCAG228200.028.6287966
ACGCAGA262700.024.8200347
CGCAGAG266250.024.5535458
GTGGTAT46000.021.3548491
GTACATG243150.021.2231661
TACATGG241100.020.834282
GCAGAGT310700.020.833729
ACATGGG246850.019.8971633
GAGTACT171900.019.42750412-13
TGGTATC46500.019.3724782
CAGAGTA303400.018.66293710-11
AGAGTAC280400.018.6333510-11
CATGGGG192000.017.895764
GTACTTT189250.017.49994714-15
Produced by FastQC (version 0.11.5)