##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139370_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7740669
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06340175506794	33.0	31.0	34.0	30.0	34.0
2	32.2404150080568	33.0	31.0	34.0	30.0	34.0
3	32.256464525223855	34.0	31.0	34.0	30.0	34.0
4	36.00061079991923	37.0	35.0	37.0	35.0	37.0
5	35.87104641730579	37.0	35.0	37.0	35.0	37.0
6	35.89438173883937	37.0	35.0	37.0	35.0	37.0
7	35.836736721335065	37.0	35.0	37.0	35.0	37.0
8	35.82532375431633	37.0	35.0	37.0	35.0	37.0
9	37.59901631241434	39.0	37.0	39.0	35.0	39.0
10-11	37.48083931763521	39.0	37.0	39.0	34.5	39.0
12-13	37.458314197390436	39.0	37.0	39.0	34.0	39.0
14-15	38.70631252931756	40.0	38.0	41.0	34.0	41.0
16-17	38.666199781956834	40.0	38.0	41.0	34.0	41.0
18-19	38.68604018593225	40.0	38.0	41.0	34.0	41.0
20-21	38.671220244971195	40.0	38.0	41.0	34.0	41.0
22-23	38.59130715855504	40.0	38.0	41.0	34.0	41.0
24-25	38.53266617833417	40.0	38.0	41.0	34.0	41.0
26-27	38.3651368023936	40.0	38.0	41.0	34.0	41.0
28-29	38.2592677600253	40.0	38.0	41.0	34.0	41.0
30-31	38.10627321492257	40.0	37.5	41.0	33.0	41.0
32-33	37.932083745162984	40.0	37.0	41.0	33.0	41.0
34-35	38.018298373686775	40.0	37.0	41.0	33.0	41.0
36-37	37.99166758456802	40.0	37.0	41.0	33.0	41.0
38-39	37.959523520878406	40.0	37.0	41.0	33.0	41.0
40-41	37.88783567359265	40.0	37.0	41.0	33.0	41.0
42-43	37.782979926553004	40.0	37.0	41.0	33.0	41.0
44-45	37.83792447815678	40.0	37.0	41.0	33.0	41.0
46-47	37.835298130167544	40.0	37.0	41.0	33.0	41.0
48-49	37.77882760637381	40.0	37.0	41.0	33.0	41.0
50-51	37.68819043445116	40.0	37.0	41.0	32.5	41.0
52-53	37.501416272094566	39.5	36.0	41.0	32.0	41.0
54-55	37.37612377352552	39.0	36.0	41.0	31.5	41.0
56-57	37.206375210100255	39.0	36.0	41.0	31.0	41.0
58-59	37.067788354779125	39.0	35.0	41.0	31.0	41.0
60-61	36.848758159061234	39.0	35.0	40.0	31.0	41.0
62-63	36.63346036063123	38.0	35.0	40.0	31.0	41.0
64-65	36.33322395234252	38.0	35.0	40.0	30.5	41.0
66-67	36.050840687015366	37.0	34.5	40.0	30.0	41.0
68-69	35.74487267028387	37.0	34.0	39.5	30.0	41.0
70-71	35.45380633391305	36.0	34.0	39.0	29.0	41.0
72-73	35.16457518262253	36.0	34.0	39.0	29.0	40.5
74-75	34.80389474787922	35.5	34.0	38.5	29.0	40.0
76-77	33.521879889262024	34.5	31.5	36.5	27.5	39.0
78-79	34.19883679491689	35.0	33.0	37.0	29.0	39.0
80-81	34.124199394713216	35.0	33.0	36.5	29.0	39.0
82-83	33.854157651640364	35.0	33.0	36.0	29.0	38.0
84-85	33.68219099541281	35.0	33.0	36.0	29.0	37.0
86-87	33.44794700305352	35.0	33.0	35.0	29.0	37.0
88-89	33.35020818457691	35.0	33.0	35.0	29.0	36.5
90-91	33.22135931387299	35.0	33.0	35.0	29.0	36.0
92-93	33.10540992089834	35.0	33.0	35.0	29.0	36.0
94-95	33.07049148733805	35.0	33.0	35.0	29.0	36.0
96-97	33.1270269095383	35.0	33.0	35.0	29.0	35.0
98-99	33.258376496428426	35.0	33.0	35.0	29.0	35.0
100	32.09744706670512	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	38.0
21	252.0
22	1180.0
23	4215.0
24	11579.0
25	24888.0
26	45041.0
27	72228.0
28	104098.0
29	140014.0
30	175895.0
31	218336.0
32	267724.0
33	336863.0
34	444635.0
35	608366.0
36	926174.0
37	1564114.0
38	1896839.0
39	866338.0
40	31847.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.313896770421266	20.337570305615703	14.731078153580782	23.617454770382253
2	14.65546453413781	21.35250842013785	41.1160973295719	22.87592971615244
3	17.94246724669405	26.449199675118518	30.87975987605206	24.72857320213537
4	12.609736962011938	18.438134998409055	39.07215254908846	29.87997549049055
5	13.122560853590304	38.06059915493093	35.66704634961138	13.149793641867388
6	29.635681360357868	38.25860529626057	18.934603714485142	13.171109628896415
7	26.349014536082088	32.97397162958396	23.214440508953423	17.462573325380532
8	24.62359519571241	36.44527365787118	21.19041648725711	17.740714659159305
9	25.752360680969566	16.87820523006474	21.14447730551455	36.22495678345115
10-11	24.149225612749078	26.750732188053266	29.028674978136575	20.071367221061077
12-13	25.09606889895879	24.331419179105783	29.048912248341342	21.52359967359408
14-15	22.173072002760485	25.691353401841198	26.760531083329557	25.375043512068757
16-17	21.704237617786426	28.887376803784825	27.32103161525355	22.0873539631752
18-19	21.727587034603	28.036777011222213	29.076936731303825	21.158699222870954
20-21	22.603728711278386	27.187603010724708	28.949037552409308	21.2596307255876
22-23	22.320466838258984	27.076316674717393	28.802675532564027	21.8005409544596
24-25	22.060674466439348	27.26765498880447	28.824904306679205	21.846766238076984
26-27	22.003182963382734	27.450916386676592	28.70600276435994	21.839897885580733
28-29	21.95252462599014	27.53546341123019	28.740252222342296	21.77175974043738
30-31	22.237057090182187	27.316220261214514	28.671872572476154	21.774850076127148
32-33	22.068114540479336	27.47362510382879	28.582937131938323	21.87532322375355
34-35	22.158695776945216	27.52569457295441	28.56229146041426	21.75331818968611
36-37	22.1564351788792	27.468624098901124	28.521393565274284	21.853547156945393
38-39	21.992860744171384	27.606571981711593	28.58086056264157	21.819706711475455
40-41	22.264023670695508	27.452447451550952	28.43753505029075	21.845993827462788
42-43	22.171718017597502	27.543343515011	28.22674568130464	22.05819278608686
44-45	22.24349424281669	27.641802232450697	28.048706317630067	22.06599720710255
46-47	22.30642942883453	27.641780024518248	27.78781800568651	22.263972540960715
48-49	22.223485928201146	27.75546760944702	27.80934709497855	22.211699367373285
50-51	22.230527985016337	27.87127180359374	27.711521924234983	22.186678287154944
52-53	22.380796988986148	27.73500859601293	27.62044542763912	22.263748987361808
54-55	22.334495548541216	27.642909020474598	27.674933605629803	22.347661825354383
56-57	22.3390154835184	27.741969277823504	27.652045724321805	22.266969514336292
58-59	22.316277151400225	27.76529118048061	27.64086978098092	22.27756188713824
60-61	22.3719927213105	27.744488906494823	27.697680182518013	22.18583818967666
62-63	22.363683400240404	27.820858299267908	27.585280720106088	22.2301775803856
64-65	22.30642003221866	27.8072559748475	27.572833495932507	22.313490497001325
66-67	22.381376659766996	27.803449131554924	27.455206081354017	22.359968127324066
68-69	22.308630211524687	27.787360778759435	27.53119260128905	22.37281640842683
70-71	22.321984777711705	27.593510816166074	27.59681199980252	22.487692406319702
72-73	22.343793567570447	27.60688254904639	27.54540996256146	22.503913920821702
74-75	22.23816613383972	27.772057232825343	27.509306944377677	22.48046968895726
76-77	22.388656924279523	27.675995839583457	27.425009736039343	22.510337500097684
78-79	22.335877488101342	27.675015081724936	27.427935417255945	22.561172012917773
80-81	22.36535081477651	27.698517965264685	27.405884236906047	22.53024698305276
82-83	22.377638003670736	27.671972369668197	27.532992760374487	22.41739686628658
84-85	22.451722714386047	27.641776294786986	27.48635307862253	22.420147912204442
86-87	22.342920125518397	27.651290250932405	27.533450132229863	22.47233949131934
88-89	22.363514087348445	27.643655440157723	27.515735119080077	22.477095353413763
90-91	22.445339609477276	27.57643266525363	27.593453874341172	22.38477385092792
92-93	22.451258992140733	27.535799653037234	27.607069542807146	22.405871812014887
94-95	22.289912682705115	27.612774841760114	27.65722357464131	22.440088900893464
96-97	22.30911919225946	27.735140358523918	27.749950742054775	22.205789707161845
98-99	23.37654021001665	29.726756151171635	27.950687046915856	18.94601659189586
100	22.419713408838142	30.776612080413564	21.655585180031252	25.148089330717045
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1496.0
1	1300.5
2	1736.5
3	2534.0
4	3379.5
5	5327.5
6	7116.0
7	8591.5
8	9843.0
9	10051.5
10	9325.5
11	8480.0
12	9077.0
13	11898.5
14	18476.0
15	28066.5
16	36399.0
17	41692.0
18	43574.0
19	41886.0
20	38307.5
21	34891.5
22	32967.0
23	34361.0
24	39626.5
25	48788.5
26	61442.5
27	75533.5
28	90518.0
29	108104.5
30	125982.5
31	143303.0
32	161478.0
33	178634.0
34	195958.5
35	212365.5
36	228948.5
37	246164.5
38	259046.5
39	269309.5
40	281902.5
41	292214.0
42	298826.0
43	305367.0
44	310133.5
45	314811.5
46	317372.0
47	317807.5
48	316956.0
49	312333.0
50	306564.0
51	298533.0
52	286944.5
53	272449.5
54	255629.5
55	237983.5
56	219318.0
57	198972.0
58	177074.5
59	153585.5
60	128703.0
61	105096.5
62	83917.0
63	65024.5
64	49384.5
65	36843.5
66	27586.0
67	21342.0
68	16329.0
69	11960.0
70	8555.5
71	6126.5
72	4383.0
73	3032.0
74	1999.0
75	1307.5
76	908.5
77	597.5
78	381.0
79	247.0
80	169.0
81	116.5
82	80.0
83	57.5
84	46.5
85	37.5
86	26.0
87	16.5
88	11.0
89	7.5
90	5.0
91	3.5
92	2.5
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	6.459390008796397E-6
12-13	6.459390008796397E-6
14-15	6.459390008796397E-6
16-17	9.043146012314957E-4
18-19	0.0016406850622342848
20-21	0.0
22-23	0.00339805069465265
24-25	2.519981840623167E-4
26-27	0.0018874289539745925
28-29	0.005160564363974993
30-31	0.0017477866224411918
32-33	0.005835925294709364
34-35	0.01128268754137175
36-37	6.129055079014278E-4
38-39	1.964607980159031E-5
40-41	0.0023892866755011034
42-43	0.005931108192482201
44-45	0.0014871224201176854
46-47	0.0017141103276693303
48-49	0.0032107186523110064
50-51	0.010170076279664682
52-53	0.006184243932937538
54-55	0.009393732639725903
56-57	0.01792306519050716
58-59	0.01182690263018964
60-61	0.01637225206151646
62-63	0.01412127636703737
64-65	0.010475524270950833
66-67	0.012915777200384523
68-69	0.00881624857967535
70-71	0.008782735029157648
72-73	0.006278543568331384
74-75	0.010980838211995072
76-77	0.011386815509009619
78-79	0.00663781796374433
80-81	0.003945136712163368
82-83	0.0022344749854053504
84-85	6.34509563367796E-5
86-87	0.003886364625474092
88-89	5.126877607027435E-4
90-91	4.957609136807537E-4
92-93	0.001369748014264539
94-95	0.0011568362294371289
96-97	3.9599799149818714E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	161.0
20-21	535.0
22-23	1207.0
24-25	2595.0
26-27	3154.0
28-29	6040.0
30-31	12449.0
32-33	16327.0
34-35	22566.0
36-37	31482.0
38-39	37604.0
40-41	41578.0
42-43	52616.0
44-45	59256.0
46-47	58085.0
48-49	52413.0
50-51	47084.0
52-53	44681.0
54-55	48680.0
56-57	52574.0
58-59	53526.0
60-61	54146.0
62-63	54472.0
64-65	57936.0
66-67	62382.0
68-69	64572.0
70-71	65286.0
72-73	63913.0
74-75	66478.0
76-77	65821.0
78-79	71727.0
80-81	73930.0
82-83	72516.0
84-85	76216.0
86-87	80740.0
88-89	90336.0
90-91	98623.0
92-93	111745.0
94-95	131617.0
96-97	309171.0
98-99	549331.0
100-101	4875098.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.43349021283981
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.72924965313443	76.65882972046522
2	7.296433684228494	12.467195915003396
3	1.7056988744228865	4.371714242821786
4	0.6104285229347264	2.086041569591288
5	0.26445287175082677	1.129656591524082
6	0.1289305007978954	0.6608989606832117
7	0.08183004090112402	0.4893718198909722
8	0.049256560562597106	0.3366527907794234
9	0.02995000905619122	0.23028604250189189
>10	0.10096660997065564	1.318716582740619
>50	0.0020494835326813486	0.11354021509989365
>100	7.056571582252576E-4	0.10661934884798922
>500	4.753154928623217E-5	0.030476200050257186
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2918780017592794E-5
2	0.0	1.2918780017592794E-5	0.0	0.0	1.2918780017592794E-5
3	0.0	1.2918780017592794E-5	0.0	0.0	1.2918780017592794E-5
4	0.0	5.1675120070371176E-5	0.0	0.0	1.2918780017592794E-5
5	0.0	5.1675120070371176E-5	0.0	0.0	1.2918780017592794E-5
6	5.1675120070371176E-5	5.1675120070371176E-5	0.0	0.0	2.5837560035185588E-5
7	5.1675120070371176E-5	5.1675120070371176E-5	0.0	2.5837560035185588E-5	2.5837560035185588E-5
8	5.1675120070371176E-5	5.1675120070371176E-5	0.0	2.5837560035185588E-5	3.875634005277838E-5
9	5.1675120070371176E-5	1.1626902015833515E-4	0.0	3.875634005277838E-5	3.875634005277838E-5
10-11	5.813451007916757E-5	1.1626902015833515E-4	0.0	3.875634005277838E-5	3.875634005277838E-5
12-13	6.459390008796397E-5	1.1626902015833515E-4	0.0	3.875634005277838E-5	3.875634005277838E-5
14-15	6.459390008796397E-5	1.679441402287063E-4	0.0	3.875634005277838E-5	4.521573006157478E-5
16-17	7.751268010555676E-5	1.8086292024629912E-4	0.0	4.521573006157478E-5	5.1675120070371176E-5
18-19	9.043146012314956E-5	1.873223102550955E-4	0.0	7.105329009676037E-5	1.0335024014074235E-4
20-21	9.043146012314956E-5	1.9378170026389192E-4	0.0	8.397207011435315E-5	1.1626902015833515E-4
22-23	9.043146012314956E-5	2.196192602990775E-4	0.0	2.131598702902811E-4	1.4210658019352075E-4
24-25	9.043146012314956E-5	2.648349903606523E-4	0.0	7.622080210379749E-4	1.6794414022870634E-4
26-27	9.043146012314956E-5	2.842131603870415E-4	0.0	0.0021768144329643857	1.6794414022870634E-4
28-29	9.043146012314956E-5	2.971319404046343E-4	0.0	0.007841699470678826	1.7440353023750273E-4
30-31	9.043146012314956E-5	3.6172584049259823E-4	0.0	0.008920417602147825	1.8086292024629912E-4
32-33	9.043146012314956E-5	3.7464462051019107E-4	0.0	0.011129528985156192	2.067004802814847E-4
34-35	9.043146012314956E-5	4.909136406685262E-4	0.0	0.014113767169220127	2.067004802814847E-4
36-37	9.043146012314956E-5	6.136420508356577E-4	0.0	0.019081038085984558	2.067004802814847E-4
38-39	9.043146012314956E-5	6.459390008796398E-4	0.0	0.03327877732531904	2.196192602990775E-4
40-41	9.043146012314956E-5	6.976141209500109E-4	0.0	0.049168876746958176	2.325380403166703E-4
42-43	9.043146012314956E-5	7.169922909764002E-4	0.0	0.059833329651481026	2.454568203342631E-4
44-45	9.043146012314956E-5	8.074237510995497E-4	0.0	0.07320426696968957	2.583756003518559E-4
46-47	9.043146012314956E-5	8.655582611787172E-4	0.0	0.08697568646844349	2.777537703782451E-4
48-49	9.689085013194596E-5	8.655582611787172E-4	0.0	0.10047581158682796	2.842131603870415E-4
50-51	1.1626902015833515E-4	0.0010335024014074235	0.0	0.11445393156586336	2.842131603870415E-4
52-53	1.1626902015833515E-4	0.0010335024014074235	0.0	0.1514985332663107	2.906725503958379E-4
54-55	1.2272841016713155E-4	0.0010657993514514054	0.0	0.19605540554698825	2.971319404046343E-4
56-57	1.2918780017592794E-4	0.0010851775214777946	0.0	0.240257011377182	3.1005072042222706E-4
58-59	1.2918780017592794E-4	0.0010851775214777946	0.0	0.268555599005719	3.229695004398199E-4
60-61	1.4210658019352075E-4	0.001136852641548166	0.0	0.2895809134843513	3.3588828045741267E-4
62-63	1.5502536021111353E-4	0.0012079059316449264	0.0	0.3064270026272923	3.3588828045741267E-4
64-65	1.5502536021111353E-4	0.0013435531218296507	0.0	0.314081379787716	3.3588828045741267E-4
66-67	1.6794414022870634E-4	0.0013435531218296507	0.0	0.3208895768569874	3.4880706047500545E-4
68-69	1.6794414022870634E-4	0.001382309461882429	0.0	0.3274264795458894	3.8756340052778385E-4
70-71	1.6794414022870634E-4	0.001414606411926411	0.0	0.3325358570428473	4.198603505717658E-4
72-73	1.8086292024629912E-4	0.001446903361970393	0.0	0.3362047905678437	4.39238520598155E-4
74-75	1.8086292024629912E-4	0.0014533627519791893	0.0	0.33927946021203076	4.521573006157478E-4
76-77	1.8086292024629912E-4	0.001479200312014375	0.0	0.3422249420560419	4.521573006157478E-4
78-79	1.8086292024629912E-4	0.0014985784820407643	0.0	0.34418859661871604	4.521573006157478E-4
80-81	1.8086292024629912E-4	0.0014985784820407643	0.0	0.3447376447694637	4.6507608063334064E-4
82-83	1.8086292024629912E-4	0.0014985784820407643	0.0	0.3451575051200355	4.909136406685262E-4
84-85	1.8086292024629912E-4	0.0014985784820407643	0.0	0.3453189898702554	4.973730306773226E-4
86-87	1.873223102550955E-4	0.0015373348220935426	0.0	0.3453965025503609	5.03832420686119E-4
88	1.9378170026389192E-4	0.0015890099421639137	0.0	0.34540942133037855	5.03832420686119E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9720	0.0	51.501923	1
GTATCAA	17490	0.0	37.94192	1
TCAACGC	21545	0.0	30.263275	4
ATCAACG	21700	0.0	30.007572	3
TATCAAC	22230	0.0	29.40792	2
CAACGCA	22185	0.0	29.332224	5
AACGCAG	22820	0.0	28.628796	6
ACGCAGA	26270	0.0	24.820034	7
CGCAGAG	26625	0.0	24.553545	8
GTGGTAT	4600	0.0	21.354849	1
GTACATG	24315	0.0	21.223166	1
TACATGG	24110	0.0	20.83428	2
GCAGAGT	31070	0.0	20.83372	9
ACATGGG	24685	0.0	19.897163	3
GAGTACT	17190	0.0	19.427504	12-13
TGGTATC	4650	0.0	19.372478	2
CAGAGTA	30340	0.0	18.662937	10-11
AGAGTAC	28040	0.0	18.63335	10-11
CATGGGG	19200	0.0	17.89576	4
GTACTTT	18925	0.0	17.499947	14-15
>>END_MODULE