FastQCFastQC Report
Fri 27 May 2016
SRR2139369_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139369_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7855297
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96750.051.430031
GTATCAA174250.037.327091
TCAACGC212000.030.0526224
ATCAACG213200.029.9438783
CAACGCA216950.029.4460875
TATCAAC225300.028.7168222
AACGCAG222950.028.692156
ACGCAGA257550.024.8042267
CGCAGAG260400.024.5327518
GCAGAGT304450.020.7575539
GTACATG231200.020.5376911
TACATGG227050.020.3647232
GTGGTAT46900.020.3219051
GAGTACT176550.019.79484412-13
TGGTATC46100.019.7432792
ACATGGG227300.019.549033
AGAGTAC275750.018.78446810-11
CAGAGTA300200.018.64178310-11
GTACTTT197350.017.70949414-15
CATGGGG171100.017.1879834
Produced by FastQC (version 0.11.5)