##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139369_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7855297
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05058752075192	33.0	31.0	34.0	30.0	34.0
2	32.229605831580905	33.0	31.0	34.0	30.0	34.0
3	32.243734896338104	34.0	31.0	34.0	30.0	34.0
4	35.99101230672755	37.0	35.0	37.0	35.0	37.0
5	35.859659411986584	37.0	35.0	37.0	35.0	37.0
6	35.881517783477825	37.0	35.0	37.0	35.0	37.0
7	35.82647823500499	37.0	35.0	37.0	35.0	37.0
8	35.81560111603673	37.0	35.0	37.0	35.0	37.0
9	37.588978112476205	39.0	37.0	39.0	35.0	39.0
10-11	37.468304190153475	39.0	37.0	39.0	34.5	39.0
12-13	37.44611610483983	39.0	37.0	39.0	34.0	39.0
14-15	38.68881144786759	40.0	38.0	41.0	34.0	41.0
16-17	38.65072822835343	40.0	38.0	41.0	34.0	41.0
18-19	38.673682739175874	40.0	38.0	41.0	34.0	41.0
20-21	38.659162905798446	40.0	38.0	41.0	34.0	41.0
22-23	38.579044887800976	40.0	38.0	41.0	34.0	41.0
24-25	38.51985261078272	40.0	38.0	41.0	34.0	41.0
26-27	38.349955772052596	40.0	38.0	41.0	34.0	41.0
28-29	38.2425827122924	40.0	38.0	41.0	33.5	41.0
30-31	38.091583724778594	40.0	37.5	41.0	33.0	41.0
32-33	37.916311086309676	40.0	37.0	41.0	33.0	41.0
34-35	38.00116071890366	40.0	37.0	41.0	33.0	41.0
36-37	37.978042887358676	40.0	37.0	41.0	33.0	41.0
38-39	37.94586532391881	40.0	37.0	41.0	33.0	41.0
40-41	37.87600686748682	40.0	37.0	41.0	33.0	41.0
42-43	37.77555187209896	40.0	37.0	41.0	33.0	41.0
44-45	37.825977190795086	40.0	37.0	41.0	33.0	41.0
46-47	37.825778225312376	40.0	37.0	41.0	33.0	41.0
48-49	37.77502088821985	40.0	37.0	41.0	33.0	41.0
50-51	37.687392945280806	40.0	37.0	41.0	32.0	41.0
52-53	37.503162345089066	40.0	36.0	41.0	32.0	41.0
54-55	37.38121966796812	39.0	36.0	41.0	31.5	41.0
56-57	37.220117362911935	39.0	36.0	41.0	31.0	41.0
58-59	37.08819962033816	39.0	35.0	41.0	31.0	41.0
60-61	36.87895647885452	39.0	35.0	40.5	31.0	41.0
62-63	36.67296663001925	38.5	35.0	40.0	31.0	41.0
64-65	36.376771251694734	38.0	35.0	40.0	30.0	41.0
66-67	36.09967900381911	37.5	34.5	40.0	30.0	41.0
68-69	35.80566847564825	37.0	34.0	40.0	30.0	41.0
70-71	35.51629374638925	36.5	34.0	39.0	29.0	41.0
72-73	35.235646327501556	36.0	34.0	39.0	29.0	40.5
74-75	34.87293862792738	35.5	34.0	38.5	29.0	40.0
76-77	33.58166779454901	34.5	31.5	36.5	27.5	39.0
78-79	34.260986377697805	35.0	33.0	37.0	29.0	39.0
80-81	34.1806172781325	35.0	33.5	37.0	29.0	39.0
82-83	33.89539403816769	35.0	33.0	36.0	29.0	38.5
84-85	33.71402518313786	35.0	33.0	36.0	29.0	37.0
86-87	33.474954598012104	35.0	33.0	35.5	29.0	37.0
88-89	33.367385827979085	35.0	33.0	35.0	29.0	36.5
90-91	33.23546269114496	35.0	33.0	35.0	29.0	36.0
92-93	33.10929214290339	35.0	33.0	35.0	29.0	36.0
94-95	33.07543733778111	35.0	33.0	35.0	29.0	36.0
96-97	33.131550524690645	35.0	33.0	35.0	29.0	35.0
98-99	33.26148657709835	35.0	33.0	35.0	29.0	35.0
100	32.08566996560313	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	6.0
20	46.0
21	254.0
22	1219.0
23	4498.0
24	11957.0
25	26139.0
26	47808.0
27	75882.0
28	107977.0
29	143476.0
30	180661.0
31	222140.0
32	272087.0
33	343025.0
34	447662.0
35	610141.0
36	921590.0
37	1555232.0
38	1951712.0
39	899745.0
40	32039.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.082342271718055	20.506366595686963	14.834194047659816	23.577097084935172
2	14.443527724031313	21.45629885159021	41.55289099826525	22.54728242611323
3	17.758870224766802	26.686693068384304	31.055210261305206	24.49922644554369
4	12.424202420354062	18.617628334103724	39.393660099675415	29.564509145866797
5	12.881944501907439	38.207339582449904	35.95671812281572	12.953997792826929
6	29.32871920692496	38.62537597241709	19.097622916103617	12.94828190455434
7	26.05556479914127	33.12452730940663	23.49058221477813	17.329325676673967
8	24.317374632684164	36.8275572521319	21.32223644758435	17.532831667599584
9	25.625689264199686	16.9570163928875	21.294535394396927	36.12275894851589
10-11	23.89059891688373	26.959177991615086	29.329890391158987	19.820332700342203
12-13	24.84048025173332	24.546201117538903	29.279866821076272	21.3334518096515
14-15	21.957323828748933	25.96992195202804	26.94523835317748	25.127515866045545
16-17	21.45215945742105	29.071590624610334	27.599023096154273	21.877226821814343
18-19	21.455743277112344	28.285341009813607	29.32082563537037	20.938090077703674
20-21	22.33636093515239	27.431285711048492	29.187688143008927	21.04466521079019
22-23	22.039210768144123	27.342528751730388	29.05412687716627	21.56413360295922
24-25	21.808597646736114	27.487634549743905	29.07585494813168	21.627912855388303
26-27	21.765822513487638	27.639652622614918	28.979635463567853	21.614889400329588
28-29	21.66554978259796	27.76543203975534	28.981749062757412	21.587269114889292
30-31	22.00286084886734	27.571590433711275	28.886899636691076	21.538649080730313
32-33	21.826090751501265	27.706959382086875	28.81758792207038	21.64936194434148
34-35	21.93674848914881	27.745576002530033	28.80150346681443	21.51617204150673
36-37	21.899913979331785	27.73024725273137	28.744540841638138	21.625297926298703
38-39	21.762260412501984	27.850515024344013	28.791422526640115	21.59580203651389
40-41	22.023299064385178	27.68988532626463	28.65327124208153	21.63354436726867
42-43	21.890486167141002	27.81219637123168	28.456637330578978	21.840680131048344
44-45	22.01906597242919	27.89816093940004	28.270794104355666	21.8119789838151
46-47	22.031775990637303	27.836144327737806	28.072407964775575	22.059671716849316
48-49	21.959256539030996	27.964894853230625	28.084497274974744	21.991351332763635
50-51	22.00499448293225	28.05180675581117	27.975593114978594	21.967605646277985
52-53	22.129820063648154	27.986677401827386	27.854677318390696	22.028825216133765
54-55	22.140236890383797	27.860666410753982	27.9021639291888	22.096932769673426
56-57	22.078992975726454	27.995955263391625	27.87591090502862	22.049140855853302
58-59	22.070180664425944	28.014516006090478	27.9034095083503	22.011893821133274
60-61	22.120792002010013	28.01091744889747	27.925393362931366	21.94289718616115
62-63	22.068493926101308	28.0566808692632	27.881092330388224	21.99373287424727
64-65	22.06316511775776	28.020654059295314	27.80412910765903	22.11205171528789
66-67	22.142668663265404	28.05592370053973	27.67532680007749	22.12608083611738
68-69	22.090306616082792	28.021293370591838	27.75661996935881	22.13178004396656
70-71	22.060754060165436	27.828246270302635	27.841291443190947	22.26970822634098
72-73	22.082554836277847	27.879237159625227	27.77596715447413	22.2622408496228
74-75	22.01503997641944	27.968262038981617	27.76016359014038	22.25653439445857
76-77	22.103452784125633	27.84451551660799	27.74914329072352	22.302888408542856
78-79	22.102196452543485	27.931086320239203	27.667101617687074	22.29961560953024
80-81	22.126623356885002	27.938525218038368	27.632297278730423	22.30255414634621
82-83	22.14070222523587	27.90945364572961	27.774531239947976	22.175312889086545
84-85	22.224552852076755	27.88132063058862	27.713577477263012	22.18054904007161
86-87	22.05824450212141	27.914780904522058	27.777794829550867	22.24917976380566
88-89	22.14256571847639	27.86916530907868	27.75829872507416	22.229970247370765
90-91	22.206753394298957	27.811334204085757	27.830474348482113	22.15143805313317
92-93	22.205322323503587	27.76078867218756	27.8507174332268	22.183171571082053
94-95	22.046368874699123	27.877879394849252	27.898264565426867	22.177487165024758
96-97	22.064694789785342	28.003346404128738	27.984576122287397	21.947382683798523
98-99	23.08947049761195	29.974870506470403	28.210356781388708	18.725302214528945
100	22.14999430502068	31.017448733635472	21.97010527576054	24.862451685583306
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1388.0
1	1228.0
2	1688.0
3	2478.0
4	3339.5
5	5345.0
6	7168.5
7	8671.0
8	9950.0
9	10275.0
10	9674.0
11	8732.0
12	9226.5
13	11844.5
14	18114.0
15	27757.0
16	36281.5
17	41779.5
18	44006.5
19	42801.0
20	39598.0
21	36340.5
22	34706.5
23	36117.0
24	41447.5
25	50870.5
26	63939.0
27	79243.5
28	95702.5
29	114554.5
30	132766.5
31	151659.5
32	173164.5
33	192454.0
34	211038.0
35	228307.5
36	245385.0
37	262433.5
38	275191.0
39	285316.0
40	297166.0
41	307869.5
42	314057.0
43	318615.0
44	321978.5
45	324992.5
46	325444.0
47	322870.5
48	319763.5
49	312734.0
50	303016.0
51	291028.0
52	275546.5
53	258533.0
54	241375.0
55	223645.0
56	204868.5
57	185124.5
58	163992.0
59	141359.5
60	118129.5
61	96433.5
62	76532.0
63	59572.5
64	45275.5
65	33784.0
66	25640.5
67	19809.5
68	15310.5
69	11330.0
70	8190.5
71	5931.5
72	4213.0
73	2930.5
74	2011.5
75	1344.5
76	865.0
77	557.5
78	346.0
79	225.0
80	157.5
81	113.0
82	85.5
83	57.5
84	36.0
85	25.0
86	23.0
87	18.0
88	10.0
89	5.5
90	4.0
91	3.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	9.102138340536328E-4
18-19	0.0017122204290938969
20-21	0.0
22-23	0.0032211468161159958
24-25	2.928948112219718E-4
26-27	0.0018026292908787595
28-29	0.005110957159281578
30-31	0.0016393732287034732
32-33	0.0053990808911384275
34-35	0.011347468317061585
36-37	6.231015476943122E-4
38-39	3.225311418331341E-5
40-41	0.0025799741756253242
42-43	0.005983406020636102
44-45	0.0017658472150915475
46-47	0.0019580206977340606
48-49	0.0028322053603451534
50-51	0.01048012245506463
52-53	0.006196612855811637
54-55	0.009914954141299778
56-57	0.018170526080481485
58-59	0.011738618084439288
60-61	0.0160076237348394
62-63	0.014442772070347967
64-65	0.01043537555698465
66-67	0.012815578290367776
68-69	0.008553297177863236
70-71	0.008561065632860148
72-73	0.005999636080468404
74-75	0.010227022209437626
76-77	0.01177783038823092
78-79	0.006922157148600375
80-81	0.004027452943011845
82-83	0.0022596724935216956
84-85	3.109809068607519E-5
86-87	0.003840436475049086
88-89	5.024140196159978E-4
90-91	6.396200236044078E-4
92-93	0.0016386347586875639
94-95	0.001309738451108345
96-97	4.4834647663903615E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	126.0
20-21	576.0
22-23	1231.0
24-25	2894.0
26-27	3297.0
28-29	5994.0
30-31	12212.0
32-33	16136.0
34-35	22122.0
36-37	30632.0
38-39	37015.0
40-41	40514.0
42-43	51345.0
44-45	57988.0
46-47	56838.0
48-49	52096.0
50-51	46245.0
52-53	43826.0
54-55	47347.0
56-57	51545.0
58-59	52474.0
60-61	53639.0
62-63	54340.0
64-65	57330.0
66-67	61981.0
68-69	64032.0
70-71	65274.0
72-73	64032.0
74-75	66197.0
76-77	65560.0
78-79	72129.0
80-81	74831.0
82-83	73184.0
84-85	76848.0
86-87	81642.0
88-89	91460.0
90-91	100903.0
92-93	113170.0
94-95	134627.0
96-97	313055.0
98-99	560541.0
100-101	4978069.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.46867878497278
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.81758642744168	76.76590443608544
2	7.1431935216203835	12.21038625196542
3	1.7522477867450696	4.492869097109815
4	0.6087427377277524	2.081137500541528
5	0.265610075821848	1.1350671126234895
6	0.14176575557049745	0.7269919095338288
7	0.08098564028479158	0.4845214972987341
8	0.050713050194263226	0.34675019178077476
9	0.03365084850759193	0.258848420574344
>10	0.10366487346422705	1.311871761871774
>50	0.001351726301111623	0.0736904500169824
>100	4.414571427753929E-4	0.06775416864424598
>500	2.3049589282990784E-5	0.01719825663849197
>1k	2.304958870493533E-5	0.027008945315057084
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.2730263413337522E-5	0.0
4	0.0	3.8190790240012565E-5	0.0	1.2730263413337522E-5	0.0
5	0.0	3.8190790240012565E-5	0.0	1.2730263413337522E-5	0.0
6	0.0	3.8190790240012565E-5	0.0	1.2730263413337522E-5	1.2730263413337522E-5
7	0.0	3.8190790240012565E-5	0.0	1.2730263413337522E-5	1.2730263413337522E-5
8	0.0	3.8190790240012565E-5	0.0	1.2730263413337522E-5	2.5460526826675045E-5
9	0.0	1.145723707200377E-4	0.0	2.5460526826675045E-5	2.5460526826675045E-5
10-11	0.0	1.145723707200377E-4	0.0	7.001644877335637E-5	2.5460526826675045E-5
12-13	1.2730263413337522E-5	1.145723707200377E-4	0.0	7.638158048002513E-5	2.5460526826675045E-5
14-15	1.2730263413337522E-5	1.59128292666719E-4	0.0	7.638158048002513E-5	3.8190790240012565E-5
16-17	1.2730263413337522E-5	1.6549342437338779E-4	0.0	1.145723707200377E-4	3.8190790240012565E-5
18-19	1.2730263413337522E-5	1.782236877867253E-4	0.0	1.7185855608005656E-4	5.7286185360018845E-5
20-21	1.2730263413337522E-5	2.0368421461340036E-4	0.0	2.546052682667504E-4	6.36513170666876E-5
22-23	1.2730263413337522E-5	2.2277960973340666E-4	0.0	4.8375000970682586E-4	6.36513170666876E-5
24-25	1.9095395120006283E-5	2.3550987314674415E-4	0.0	0.001094802653547027	6.36513170666876E-5
26-27	2.5460526826675045E-5	2.4187500485341293E-4	0.0	0.0024251151802407982	6.36513170666876E-5
28-29	2.5460526826675045E-5	2.4187500485341293E-4	0.0	0.007370822516322425	8.274671218669389E-5
30-31	2.5460526826675045E-5	2.800657950934255E-4	0.0	0.008160098847949352	8.911184389336265E-5
32-33	3.8190790240012565E-5	2.800657950934255E-4	0.0	0.009929605462403268	8.911184389336265E-5
34-35	3.8190790240012565E-5	3.18256585333438E-4	0.0	0.012596595647497478	1.0184210730670018E-4
36-37	3.8190790240012565E-5	3.628125072801194E-4	0.0	0.01734498390067237	1.0184210730670018E-4
38-39	3.8190790240012565E-5	4.073684292268007E-4	0.0	0.030495346006650034	1.0184210730670018E-4
40-41	3.8190790240012565E-5	4.4555921946681326E-4	0.0	0.045345198278308255	1.0184210730670018E-4
42-43	3.8190790240012565E-5	4.710197462934883E-4	0.0	0.05583493533089837	1.0184210730670018E-4
44-45	4.455592194668133E-5	5.474013267735135E-4	0.0	0.0680941789979424	1.0184210730670018E-4
46-47	5.092105365335009E-5	5.983223804268636E-4	0.0	0.08055074174789317	1.0820723901336894E-4
48-49	5.092105365335009E-5	5.983223804268636E-4	0.0	0.09359289661485747	1.145723707200377E-4
50-51	5.092105365335009E-5	7.383552779735762E-4	0.0	0.1066032258232884	1.145723707200377E-4
52-53	5.7286185360018845E-5	7.510855413869138E-4	0.0	0.14170056205385995	1.145723707200377E-4
54-55	7.001644877335637E-5	7.638158048002513E-4	0.0	0.18291478985454018	1.145723707200377E-4
56-57	7.638158048002513E-5	7.765460682135889E-4	0.0	0.2253320275477808	1.145723707200377E-4
58-59	7.638158048002513E-5	7.765460682135889E-4	0.0	0.25389237351560356	1.145723707200377E-4
60-61	7.638158048002513E-5	7.956414633335952E-4	0.0	0.2742926206354769	1.273026341333752E-4
62-63	7.638158048002513E-5	8.020065950402639E-4	0.0	0.2907719466240424	1.273026341333752E-4
64-65	7.638158048002513E-5	8.911184389336265E-4	0.0	0.2974871605745779	1.273026341333752E-4
66-67	7.638158048002513E-5	8.911184389336265E-4	0.0	0.30378864096417996	1.3366776584004396E-4
68-69	7.638158048002513E-5	9.038487023469641E-4	0.0	0.30992462792940867	1.5276316096005026E-4
70-71	7.638158048002513E-5	9.165789657603016E-4	0.0	0.31467301618258353	1.6549342437338779E-4
72-73	7.638158048002513E-5	9.293092291736392E-4	0.0	0.3186830491577849	1.6549342437338779E-4
74-75	7.638158048002513E-5	9.484046242936453E-4	0.0	0.3218974406696526	1.6549342437338779E-4
76-77	7.638158048002513E-5	0.001012055941360333	0.0	0.32481903612301355	1.6549342437338779E-4
78-79	8.911184389336265E-5	0.0010311513364803394	0.0	0.32669674997648085	1.6549342437338779E-4
80-81	8.911184389336265E-5	0.0010311513364803394	0.0	0.3273141677520277	1.6549342437338779E-4
82-83	8.911184389336265E-5	0.001037516468187008	0.0	0.32775336183978787	1.6549342437338779E-4
84-85	8.911184389336265E-5	0.0010438815998936768	0.0	0.3279315855275746	1.6549342437338779E-4
86-87	8.911184389336265E-5	0.0010438815998936768	0.0	0.3280143322397613	1.6549342437338779E-4
88	8.911184389336265E-5	0.0010820723901336894	0.0	0.32802069737146794	1.6549342437338779E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9675	0.0	51.43003	1
GTATCAA	17425	0.0	37.32709	1
TCAACGC	21200	0.0	30.052622	4
ATCAACG	21320	0.0	29.943878	3
CAACGCA	21695	0.0	29.446087	5
TATCAAC	22530	0.0	28.716822	2
AACGCAG	22295	0.0	28.69215	6
ACGCAGA	25755	0.0	24.804226	7
CGCAGAG	26040	0.0	24.532751	8
GCAGAGT	30445	0.0	20.757553	9
GTACATG	23120	0.0	20.537691	1
TACATGG	22705	0.0	20.364723	2
GTGGTAT	4690	0.0	20.321905	1
GAGTACT	17655	0.0	19.794844	12-13
TGGTATC	4610	0.0	19.743279	2
ACATGGG	22730	0.0	19.54903	3
AGAGTAC	27575	0.0	18.784468	10-11
CAGAGTA	30020	0.0	18.641783	10-11
GTACTTT	19735	0.0	17.709494	14-15
CATGGGG	17110	0.0	17.187983	4
>>END_MODULE