FastQCFastQC Report
Fri 27 May 2016
SRR2139368_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139368_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8107621
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98700.051.4416851
GTATCAA178900.037.7851
TCAACGC214950.030.9500394
ATCAACG216750.030.7127653
CAACGCA221800.029.9362835
TATCAAC230150.029.0361822
AACGCAG228800.029.02046
ACGCAGA264700.025.0521567
CGCAGAG268300.024.7319668
GCAGAGT317200.020.7303039
GTGGTAT44250.020.4134671
GTACATG238200.020.290831
TACATGG235300.020.0132852
GAGTACT178600.019.80035812-13
ACATGGG238150.019.0906873
CAGAGTA307900.018.6107710-11
AGAGTAC283700.018.56854810-11
TGGTATC44050.018.2708852
GTACTTT197700.017.93178614-15
CATGGGG177550.016.926384
Produced by FastQC (version 0.11.5)