FastQCFastQC Report
Fri 27 May 2016
SRR2139367_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139367_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10343077
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119650.048.299251
GTATCAA217300.036.6414451
TCAACGC257900.030.2258114
ATCAACG259300.030.013093
CAACGCA263800.029.533575
TATCAAC273800.028.7050692
AACGCAG273950.028.4549626
ACGCAGA312300.025.0018547
CGCAGAG316050.024.637488
GCAGAGT380600.020.4252039
TACATGG284150.020.337872
GTACATG291500.020.1628231
ACATGGG286150.019.657173
GTGGTAT65600.019.5113221
GAGTACT213350.019.11182812-13
TGGTATC63150.018.7091942
AGAGTAC338500.018.60904510-11
CAGAGTA370450.018.02097510-11
CATGGGG206250.017.5795924
GTACTTT242250.016.90311614-15
Produced by FastQC (version 0.11.5)